Home

Grb2 (human)

Overview Grb2 an adaptor protein of the GRB2 / sem-5 / DRK family. Associates with many different tyrosine-phosphorylated proteins via its SH2 domain, including EGFR, PDGFR, IRS-1, SHC and LNK. It also seems to interact with RAS in the signaling pathway leading to DNA synthesis. Binds to and translocates the guanine nucleotide exchange factors SOS. Four alternatively spliced isoforms have been described. Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. Note: This description may include information from UniProtKB. Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold; Cell development/differentiation Cellular Component: signalosome; Golgi apparatus; cytoplasm; plasma membrane; vesicle membrane; nucleus; cytosol; endosome Molecular Function: identical protein binding; protein domain specific binding; protein binding; SH3/SH2 adaptor activity; ephrin receptor binding; phosphotyrosine binding; neurotrophin TRKA receptor binding; insulin receptor substrate binding; protein phosphatase binding; protein kinase binding; epidermal growth factor receptor binding Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; platelet activation; axon guidance; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; protein heterooligomerization; DNA damage response, signal transduction; cell-cell signaling; positive regulation of actin filament polymerization; virus-host interaction; receptor internalization; regulation of MAPKKK cascade; Ras protein signal transduction; T cell costimulation; insulin receptor signaling pathway; blood coagulation; leukocyte migration; aging Reference #:  P62993 (UniProtKB) Alt. Names/Synonyms: abundant SRC homology; Adapter protein GRB2; ASH; EGFRBP-GRB2; epidermal growth factor receptor-binding protein GRB2; GRB2; Grb3-3; Growth factor receptor-bound protein 2; growth factor receptor-bound protein 3; HT027; MST084; MSTP084; NCKAP2; Protein Ash; SH2/SH3 adapter GRB2 Gene Symbols: GRB2 Molecular weight: 25,206 Da Basal Isoelectric point: 5.89  Predict pI for various phosphorylation states CST Pathways:  B Cell Receptor Signaling  |  ErbB/HER Signaling  |  GPCRs Signaling to MAPK/Erk  |  Insulin Receptor Signaling  |  Jak/Stat/IL-6 Signaling  |  MAPK/Erk in Growth and Differentiation  |  Regulation of Actin Dynamics  |  SAPK/JNK Signaling Cascades  |  T Cell Receptor Signaling  |  TGF-ß Signaling Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below Grb2 1AZE - A=1-56 (human) 1BM2 - A=49-163 (human) 1BMB - A=49-168 (human) 1CJ1 - A/B/C/D/E/F/G/H/I/J/K/L=57-152 (human) 1FHS - A=53-163 (human) 1FYR - A/B/C/D=50-161 (human) 1GCQ - A/B=159-217 (human) 1GFC - A=159-215 (human) 1GFD - A=159-215 (human) 1GHU - A=60-158 (human) 1GRI - A/B=1-217 (human) 1IO6 - A=159-215 (human) 1JYQ - A/B=60-151 (human) 1JYR - A=60-151 (human) 1JYU - A=60-151 (human) 1QG1 - E=58-159 (human) 1TZE - E=55-152 (human) 1X0N - A=58-159 (human) 1ZFP - E=56-153 (human) 2AOA - A/B=55-153 (human) 2AOB - A/B/C/D=55-153 (human) 2H46 - E=53-162 (human) 2H5K - A/B=53-162 (human) 2HUW - A/B=53-162 (human) 2HUY - A=53-162 (human) 2VVK - A=161-214 (human) 2VWF - A=159-214 (human) 2W0Z - A=159-214 (human) 3C7I - A=53-162 (human) 3IMD - A/B=53-163 (human) 3IMJ - A/B=53-163 (human) 3IN7 - A/C=53-163 (human) 3IN8 - A=53-163 (human) 3KFJ - A=53-163 (human) 3MXC - A=55-152 (human) 3MXY - A=55-152 (human) 3N7Y - A/C/B=55-152 (human) 3N84 - F/A/D/C/E/B=52-162 (human) 3N8M - A=53-163 (human) 3OV1 - A=53-163 (human) 3OVE - A=53-163 (human) 3S8L - A=53-163 (human) 3S8N - A=53-163 (human) 3S8O - A=53-163 (human) 1GBQ - A=1-61 (mouse) 1GBR - A=1-61 (mouse) 2GBQ - A=1-61 (mouse) 3GBQ - A=1-61 (mouse) 4GBQ - A=1-61 (mouse)

STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 1AZE 1BM2 1BMB 1CJ1 1FHS 1FYR 1GCQ 1GFC 1GFD 1GHU 1GRI 1IO6 1JYQ 1JYR 1JYU 1QG1 1TZE 1X0N 1ZFP 2AOA 2AOB 2H46 2H5K 2HUW 2HUY 2VVK 2VWF 2W0Z 3C7I 3IMD 3IMJ 3IN7 3IN8 3KFJ 3MXC 3MXY 3N7Y 3N84 3N8M 3OV1 3OVE 3S8L 3S8N 3S8O  |  Phospho3D  |  DISEASE  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene

	Sites Implicated In
cell growth, altered: Y7‑p, Y37‑p, Y52‑p, Y209‑p molecular association, regulation: Y209‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		K6-a	__MEAIAkyDFKATA
1	2		Y7-p	_MEAIAkyDFKATAD
0	1		K10	AIAkyDFKATADDEL
0	1		S18-p	ATADDELsFkRGDIL
0	1		K20-u	ADDELsFkRGDILKV
1	0		Y37-p	EECDQNWykAELNGk
0	1		K38	ECDQNWyKAELNGkD
0	1		K38-a	ECDQNWykAELNGkD
0	3		K44-u	ykAELNGkDGFIPkN
0	1		K50-a	GkDGFIPkNyIEMkP
1	0		Y52-p	DGFIPkNyIEMkPHP
0	1		K56-u	PkNyIEMkPHPWFFG
0	1		K69-u	FGKIPRAkAEEMLSk
0	1		K76-u	kAEEMLSkQRHDGAF
0	56		K109-u	GNDVQHFkVLRDGAG
0	1		K109-a	GNDVQHFkVLRDGAG
0	1		K117-a	VLRDGAGkYFLWVVK
0	2		T159-p	EQVPQQPtyVQALFD
2	40		Y160-p	QVPQQPtyVQALFDF
3	180		Y209-p	TGMFPRNyVtPVNRN
0	4		T211-p	MFPRNyVtPVNRNV_

	mouse
K6	__MEAIAKYDFkATA
Y7	_MEAIAKYDFkATAD
K10-u	AIAKYDFkATADDEL
S18-p	ATADDELsFKRGDIL
K20	ADDELsFKRGDILKV
Y37	EECDQNWYkAELNGk
K38-u	ECDQNWYkAELNGkD
K38	ECDQNWYKAELNGkD
K44-u	YkAELNGkDGFIPKN
K50	GkDGFIPKNYIEMKP
Y52	DGFIPKNYIEMKPHP
K56	PKNYIEMKPHPWFFG
K69	FGKIPRAKAEEMLSK
K76	KAEEMLSKQRHDGAF
K109-u	GNDVQHFkVLRDGAG
K109	GNDVQHFKVLRDGAG
K117	VLRDGAGKYFLWVVK
T159	EQMPQQPTyVQALFD
Y160-p	QMPQQPTyVQALFDF
Y209-p	TGMFPRNyVTPVNRN
T211	MFPRNyVTPVNRNV_

	rat
K6	__MEAIAKYDFKATA
Y7	_MEAIAKYDFKATAD
K10	AIAKYDFKATADDEL
S18	ATADDELSFKRGDIL
K20	ADDELSFKRGDILKV
Y37	EECDQNWYKAELNGK
K38	ECDQNWYKAELNGKD
K38	ECDQNWYKAELNGKD
K44	YKAELNGKDGFIPKN
K50	GKDGFIPKNYIEMKP
Y52	DGFIPKNYIEMKPHP
K56	PKNYIEMKPHPWFFG
K69	FGKIPRAKAEEMLSK
K76	KAEEMLSKQRHDGAF
K109	GNDVQHFKVLRDGAG
K109	GNDVQHFKVLRDGAG
K117	VLRDGAGKYFLWVVK
T159	EQVPQQPTyVQALFD
Y160-p	QVPQQPTyVQALFDF
Y209	TGMFPRNYVTPVNRN
T211	MFPRNYVTPVNRNV_

Home  |  Curator Login With enhanced literature mining using Linguamatics I2E Produced by 3rd Millennium  |  Design by Digizyme ©2003-2013 Cell Signaling Technology, Inc.