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Protein Page:
BLNK (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
BLNK an adaptor protein that bridges the B-cell receptor-associated kinases (BCR) with a multitude of signaling pathways, regulating biologic outcomes of B-cell function and development. Plays an important role in BCR-mediated PLCG1 activation and Ca(2 ) mobilization. Does not seem to be not required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Its phosphorylation is required for both Ca(2 ) and MAPK signaling pathways. Defects in BLNK are the cause of hypoglobulinemia and absent B-cells.It has tumor supressor activity that is lost in many cases of childhood pre-B acute lymphoblastic leukemia (ALL). Two alternatively spliced isoforms have been described; these are differentially involved in activation and apoptosis of B lymphocytes. Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 10q23.2-q23.33
Cellular Component: plasma membrane; intracellular; cytosol
Molecular Function: protein binding; SH3/SH2 adaptor activity; transmembrane receptor protein tyrosine kinase adaptor protein activity
Biological Process: positive regulation of signal transduction; B cell differentiation; inflammatory response; humoral immune response; transmembrane receptor protein tyrosine kinase signaling pathway
Reference #:  Q8WV28 (UniProtKB)
Alt. Names/Synonyms: B cell linker protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; B-cell linker; B-cell linker protein; BASH; BLNK; BLNK-S; Cytoplasmic adapter protein; LY57; MGC111051; SLP-65; SLP65; Src homology 2 domain-containing leukocyte protein of 65 kDa
Gene Symbols: BLNK
Molecular weight: 50,466 Da
Basal Isoelectric point: 8.18  Predict pI for various phosphorylation states
CST Pathways:  B Cell Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

BLNK

Protein Structure Not Found.


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Sites Implicated In
transcription, altered: Y72‑p, Y84‑p, Y96‑p, Y178‑p, Y189‑p
molecular association, regulation: Y72‑p, Y84‑p, Y96‑p, Y178‑p, Y189‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 G30 HDIKNNEGGIMNKIK
1 0 K37 GGIMNKIKKLKVKAP
1 0 K38 GIMNKIKKLKVKAPP
0 1 - gap
1 0 K42 KIKKLKVKAPPSVPR
0 1 S46 LKVKAPPSVPRRDYA
0 2 - gap
0 1 - gap
0 1 S65 ADEEEQWSDDFDSDy
3 1 Y72-p SDDFDSDyENPDEHS
4 1 Y84-p EHSDSEMyVMPAEEN
0 1 S95 AEENADDSyEPPPVE
4 1 Y96-p EENADDSyEPPPVEQ
0 1 A111 ETRPVHPALPFARGE
0 1 A115 VHPALPFARGEYIDN
0 1 Y119 LPFARGEYIDNRSSQ
0 2 S129-p NRSSQRHsPPFsKTL
0 1 S133-p QRHsPPFsKTLPSKP
1 1 S151 SEKARLTSTLPALTA
1 0 T152 EKARLTSTLPALTAL
1 1 T157 TSTLPALTALQKPQV
0 1 L172 PPKPKGLLEDEADyV
2 8 Y178-p LLEDEADyVVPVEDN
1 5 Y189-p VEDNDENyIHPTESS
0 1 T193 DENyIHPTESSSPPP
0 2 S195 NyIHPTESSSPPPEK
0 2 S196 yIHPTESSSPPPEKA
0 1 S197 IHPTESSSPPPEKAP
0 4 S219 PNSSTPASPPGTASG
0 1 - gap
0 1 - gap
0 1 - gap
0 1 - gap
0 1 R227 PPGTASGRNSGAWET
0 1 S229 GTASGRNSGAWETKS
0 1 T234 RNSGAWETKSPPPAA
0 1 S243 SPPPAAPSPLPRAGK
0 1 T253-p PRAGKKPttPLKTTP
0 2 T254-p RAGKKPttPLKTTPV
0 1 S284 PAERHRGSSHRQEAV
0 1 S285 AERHRGSSHRQEAVQ
0 1 A299 QSPVFPPAQKQIHQK
0 1 Q302 VFPPAQKQIHQKPIP
0 1 I303 FPPAQKQIHQKPIPL
0 1 - gap
0 1 P326 PTVDGPLPSFSSNST
0 1 S327 TVDGPLPSFSSNSTI
0 1 S329 DGPLPSFSSNSTISE
0 1 S330 GPLPSFSSNSTISEQ
0 1 S332 LPSFSSNSTISEQEA
0 1 T333 PSFSSNSTISEQEAG
0 1 Y347 GVLCKPWYAGACDRK
0 1 A350 CKPWYAGACDRKSAE
0 1 S355 AGACDRKSAEEALHR
0 1 H361 KSAEEALHRSNKDGS
0 1 S375 SFLIRKSSGHDSKQP
0 1 A403 IPVRFIEATkQYALG
0 2 K405-ac VRFIEATkQYALGRK
0 1 Y451 KDSTRLKYAVKVS__
0 1 S456 LKYAVKVS_______
3601 : Phospho-BLNK (Tyr96) Antibody
  BLNK iso3  
G30 HDIKNNEGGIMNKIK
K37 GGIMNKIKKLKVKAP
K38 GIMNKIKKLKVKAPP
- gap
K42 KIKKLKVKAPPSVPR
S46 LKVKAPPSVPRRDYA
S79-p ATMKIQMstRTQRCT
T80-p TMKIQMstRTQRCT_
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- under review  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  mouse

 
G30 HDIKNNEGGIMDKIk
K37-ub GGIMDKIkkLKVkGP
K38-ub GIMDKIkkLKVkGPP
- gap
K42-ub KIkkLKVkGPPSVPR
S46 LKVkGPPSVPRRDYA
- gap
- gap
S65 ADEEEQWSDDFDSDy
Y72-p SDDFDSDyENPDEHS
Y84-p EHSDSEMyVMPAEET
S95 AEETGDDSyEPPPAE
Y96-p EETGDDSyEPPPAEQ
A111 QTRVVHPALPFTRGE
T115 VHPALPFTRGEYVDN
Y119 LPFTRGEYVDNRSSQ
S129-p NRSSQRHsPPFSKTL
S133 QRHsPPFSKTLPSKP
S151 SAKARLAStLPAPNS
T152-p AKARLAStLPAPNSL
S158 StLPAPNSLQKPQVP
L172 PPKPKDLLEDEADyV
Y178-p LLEDEADyVVPVEDN
Y189-p VEDNDENyIHPREss
R193 DENyIHPREssPPPA
S195-p NyIHPREssPPPAEK
S196-p yIHPREssPPPAEKA
P197 IHPREssPPPAEKAP
S220-p NSSSKHMsPPGTVAG
- gap
- gap
- gap
- gap
R228 PPGTVAGRNsGVWDs
S230-p GTVAGRNsGVWDsKS
S235-p RNsGVWDsKSSLPAA
S244 SSLPAAPSPLPRAGK
A254 PRAGKKPATPLKTTP
T255 RAGKKPATPLKTTPV
S285 PAERHRGSSHRQDTV
S286 AERHRGSSHRQDTVQ
T300 QSPVFPPTQKPVHQK
P303 VFPPTQKPVHQKPVP
V304 FPPTQKPVHQKPVPL
- gap
H327 PAADGPFHSFPFNST
S328 AADGPFHSFPFNSTF
P330 DGPFHSFPFNSTFAD
F331 GPFHSFPFNSTFADQ
S333 FHSFPFNSTFADQEA
T334 HSFPFNSTFADQEAE
Y348 ELLGKPWYAGACDRK
A351 GKPWYAGACDRKSAE
S356 AGACDRKSAEEALHR
H362 KSAEEALHRSNKDGS
S376 SFLIRKSSGHDSKQP
A404 IPVRFIEATKQYALG
K406 VRFIEATKQYALGKK
Y452 KDSTRLKYAVKVS__
S457 LKYAVKVS_______
3601 : Phospho-BLNK (Tyr96) Antibody
  rat

 
G30 HDIKNNEGGIMDKIK
K37 GGIMDKIKKLKVKGP
K38 GIMDKIKKLKVKGPP
- gap
K42 KIKKLKVKGPPSVPR
S46 LKVKGPPSVPRRDYA
- gap
- gap
S65 ADEEEQWSDDFDSDY
Y72 SDDFDSDYENPDEHS
Y84 EHSDSEMYVMPAEET
S95 AEETGDDSYEPPPAE
Y96 EETGDDSYEPPPAEQ
A111 QTRVVHPALPFTRGE
T115 VHPALPFTRGEYVDN
Y119 LPFTRGEYVDNRSSQ
S129 NRSSQRHSPPFSKTL
S133 QRHSPPFSKTLPSKP
S151 SAKARLASTLPAPNS
T152 AKARLASTLPAPNSL
S158 STLPAPNSLQKPQVP
L172 PPKPKDLLEDEADYV
Y178 LLEDEADYVVPVEDN
Y189 VEDNDENYIHPRESs
R193 DENYIHPRESsPLPA
S195 NYIHPRESsPLPAEK
S196-p YIHPRESsPLPAEKA
P197 IHPRESsPLPAEKAP
S220 NSSSKHVSPPGTVAG
- gap
- gap
- gap
- gap
R228 PPGTVAGRNSGVWDS
S230 GTVAGRNSGVWDSKS
S235 RNSGVWDSKSSLPAA
S244 SSLPAAPSPLPRAGK
T253 LPRAGKKTATPLKTT
T255 RAGKKTATPLKTTPV
S285 PAERHRGSSHRQDTV
S286 AERHRGSSHRQDTVQ
T300 QSPVFPPTQKPVLQK
P303 VFPPTQKPVLQKPVP
V304 FPPTQKPVLQKPVPL
- gap
P327 PAADGPVPSFPFNST
S328 AADGPVPSFPFNSTF
P330 DGPVPSFPFNSTFAD
F331 GPVPSFPFNSTFADQ
S333 VPSFPFNSTFADQEA
T334 PSFPFNSTFADQEAE
Y348 ELHGKPWYAGACDRK
A351 GKPWYAGACDRKSAE
S356 AGACDRKSAEEALHR
H362 KSAEEALHRSNKDGS
S376 SFLIRKSSGHDSKQP
A404 IPVRFIEATKQYALG
K406 VRFIEATKQYALGKK
Y452 KDSTRLKYAVKVS__
S457 LKYAVKVS_______
  chicken

 
S30-p HDIKKNEsGIINKFK
K37 sGIINKFKKFQNEQV
K38 GIINKFKKFQNEQVA
T54-p ICKTGKDtWDRLKKK
K61 tWDRLKKKPPPsLPR
S65-p LKKKPPPsLPRRDYA
- gap
- gap
S84-p DNEEEQWsDDFDSDy
Y91-p sDDFDSDyENPDGHS
Y103 GHSDSEMYVVPSEEN
S114-p SEENPDDsyEPPPSE
Y115-p EENPDDsyEPPPSEQ
S130-p EKKKIPSsFPIsRGE
S134-p IPSsFPIsRGEyADN
Y138-p FPIsRGEyADNRTSH
L148 NRTSHHQLPPINKPL
- under review  
S170-p LPRPKKPsLPsPAAK
- gap
S173-p PKKPsLPsPAAKPKL
S188-p PLKPRECsDDEDNyI
Y194-p CsDDEDNyIVPVDND
Y205-p VDNDDDNyIEPtEss
T209-p DDNyIEPtEsstPPP
S211-p NyIEPtEsstPPPAK
S212-p yIEPtEsstPPPAKP
T213-p IEPtEsstPPPAKPP
T235-p PAKSALPtPPKPSLA
Y249-p ASDMQEVyEVPEEEE
S259-p PEEEEELsPPPVTRF
S285-p AEHSHMHsMTREsPK
S290-p MHsMTREsPKLDASR
S323-p NNNDENHsFSNTQES
S325 NDENHsFSNTQESKF
S330 sFSNTQESKFPPGAA
S339-p FPPGAAPsPLPRALK
T348 LPRALKKTSNAVNPA
S349 PRALKKTSNAVNPAK
S381-p AADRRRGssHEFPLP
S382-p ADRRRGssHEFPLPP
T394-p LPPLPSGtPKssLQK
S397-p LPSGtPKssLQKPLV
S398-p PSGtPKssLQKPLVL
S419-p PSRALGTsPHssIss
S422-p ALGTsPHssIssIss
S423-p LGTsPHssIssIssT
S425-p TsPHssIssIssTAD
S426-p sPHssIssIssTADQ
S428-p HssIssIssTADQDA
S429-p ssIssIssTADQDAG
Y443-p GVHSKAWyAAtCDRK
T446-p SKAWyAAtCDRKtAE
T451-p AAtCDRKtAEDALyR
Y457-p KtAEDALyRSNKDGS
S471-p SFLIRKSsGQDSRQP
S499-p IPIRFIEsTRQYALG
R501 IRFIEsTRQYALGRE
Y547-p KDSTKLKyIVRVs__
S552-p LKyIVRVs_______
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