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Protein Page:
HK1 (mouse)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
HK1 a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). HK-2 and its mitochondrial receptor (VDAC) play the most pivotal and direct roles in the ""Warburg effect"". Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Four human isoforms are produced by alternative splicing and alternative initiation. Isoform HK1 is markedly elevated in rapidly growing tumor cells exhibiting high glucose catabolic rates. HK1 is present in most tissues but is especially prominent in brain and kidney. Isoform HK1-SC is is an integral membrane protein detected in round spermatids, condensing spermatids and mature sperm where it is found in the head membranes, mitochondria of the midpiece and the fibrous sheath of the flagellum. Isoform HK1-SA is first expressed during meiosis and continues to be present in postmeiotic germ cells while isoform HK1-SB is present only in postmeiotic germ cells. Note: This description may include information from UniProtKB.
Protein type: Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose; Kinase, other; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Mitochondrial; Carbohydrate Metabolism - galactose; EC 2.7.1.1
Cellular Component: mitochondrial outer membrane; membrane; mitochondrion; cytosol; cilium; lipid raft
Molecular Function: transferase activity; protein binding; hexokinase activity; mannokinase activity; fructokinase activity; nucleotide binding; kinase activity; glucokinase activity; phosphotransferase activity, alcohol group as acceptor; catalytic activity; ATP binding
Biological Process: glycolysis; metabolic process; carbohydrate phosphorylation; carbohydrate metabolic process; glucose 6-phosphate metabolic process; cell glucose homeostasis; phosphorylation
Reference #:  P17710 (UniProtKB)
Alt. Names/Synonyms: BB404130; dea; downeast anemia; hexokinase 1; Hexokinase type I; Hexokinase, tumor isozyme; Hexokinase-1; HK I; Hk-1; Hk1; Hk1-s; HXK1; mHk1-s
Gene Symbols: Hk1
Molecular weight: 108,303 Da
Basal Isoelectric point: 6.37  Predict pI for various phosphorylation states
CST Pathways:  Warburg Effect
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

HK1

Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  ENZYME  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

► Hide Isoforms
 
0 1 - gap
0 2 Y81 TEEKIDKYLyAMRLS
0 69 Y83-p EKIDKYLyAMRLSDE
0 1 K118-u YNPTASVkMLPTFVR
0 1 K133-u SIPDGSEkGDFIALD
0 1 S144-p IALDLGGssFRILRV
0 1 S145-p ALDLGGssFRILRVQ
0 1 K203-u KRKIKDKkLPVGFTF
0 2 K243-u VEGADVVkLLNKAIK
0 1 K247 DVVkLLNKAIKKRGD
0 1 S354 QLFEKMVSGMYMGEL
0 1 K371-u LILVKMAkESLLFEG
0 4 K389-u PELLTRGkFTTsDVA
0 2 S393-p TRGkFTTsDVAAIET
0 1 A396 kFTTsDVAAIETDkE
0 3 K402-u VAAIETDkEGVQNAK
0 2 K474-u GVDGSLYkMHPQYSR
0 2 S503-p VRFLLSEsGSGkGAA
0 1 K507-u LSEsGSGkGAAMVTA
0 111 Y517-p AMVTAVAyRLAEQHR
0 1 S531-p RQIEETLsHFRLSKQ
0 1 K544-u KQALMEVkKKLRsEM
0 1 S549-p EVkKKLRsEMEMGLR
0 2 K566-u TNSRATVkMLPSYVR
0 1 K680-u ITWTKGFkATDCVGH
0 31 Y788 FDKVVDEYSLNSGKQ
0 6 Y805 EKMISGMYLGEIVRN
0 10 K819-a NILIDFTkKGFLFRG
0 2 K819-u NILIDFTkKGFLFRG
0 1 K833-u GQISEPLkTRGIFET
0 1 T877 DDSILVKTVCGVVSk
0 1 S883 KTVCGVVSkRAAQLC
0 2 K884-a TVCGVVSkRAAQLCG
0 8 K884-u TVCGVVSkRAAQLCG
0 3 K900-a GMAAVVEkIRENRGL
0 1 K900-u GMAAVVEkIRENRGL
0 1 K936-a RIMHQTVkELSPkCT
0 1 K941-u TVkELSPkCTVSFLL
  HK1 iso3  
S8-p MDSEHCPsVPCGGAG
Y24 WEARIDKYLYAMRLS
Y26 ARIDKYLYAMRLSDE
K61 YNPTASVKMLPTFVR
K76 SIPDGSEKGDFIALD
S87 IALDLGGSSFRILRV
S88 ALDLGGSSFRILRVQ
K146 KRKIKDKKLPVGFTF
K186 VEGADVVKLLNKAIK
K190 DVVKLLNKAIKKRGD
S297 QLFEKMVSGMYMGEL
K314 LILVKMAKESLLFEG
K332 PELLTRGKFTTSDVA
S336 TRGKFTTSDVAAIET
A339 KFTTSDVAAIETDKE
K345 VAAIETDKEGVQNAK
K417 GVDGSLYKMHPQYSR
S446 VRFLLSESGSGKGAA
K450 LSESGSGKGAAMVTA
Y460 AMVTAVAYRLAEQHR
S474 RQIEETLSHFRLSKQ
K487 KQALMEVKKKLRSEM
S492 EVKKKLRSEMEMGLR
K509 TNSRATVKMLPSYVR
K623 ITWTKGFKATDCVGH
Y731 FDKVVDEYSLNSGKQ
Y748 EKMISGMYLGEIVRN
K762 NILIDFTKKGFLFRG
K762 NILIDFTKKGFLFRG
K776 GQISEPLKTRGIFET
T820 DDSILVKTVCGVVSK
S826 KTVCGVVSKRAAQLC
K827 TVCGVVSKRAAQLCG
K827 TVCGVVSKRAAQLCG
K843 GMAAVVEKIRENRGL
K843 GMAAVVEKIRENRGL
K879 RIMHQTVKELSPKCT
K884 TVKELSPKCTVSFLL
  human

 
- gap
Y25-p QVKKIDKyLyAMRLS
Y27-p KKIDKyLyAMRLSDE
K62 FNPTATVKMLPTFVR
K77 SIPDGSEKGDFIALD
S88 IALDLGGSSFRILRV
S89 ALDLGGSSFRILRVQ
K147 KRKIKDKKLPVGFTF
K187-u VEGADVVkLLNkAIK
K191-u DVVkLLNkAIKKRGD
S298-p QLFEKMVsGMYLGEL
K315 LILVKMAKEGLLFEG
K333-u PELLTRGkFNTsDVs
S337-p TRGkFNTsDVsAIEK
S340-p kFNTsDVsAIEKNkE
K346-u VsAIEKNkEGLHNAK
K418-u GVDGSLYkTHPQYSR
S447 VRFLLSESGSGKGAA
K451 LSESGSGKGAAMVTA
Y461-p AMVTAVAyRLAEQHR
A475 RQIEETLAHFHLTKD
K488 KDMLLEVKKRMRAEM
A493 EVKKRMRAEMELGLR
K510-u THNNAVVkMLPSFVR
K624 ITWTKGFKATDCVGH
Y732-p YDRLVDEySLNAGKQ
Y749-p EKMISGMyLGEIVRN
K763-a NILIDFTkKGFLFRG
K763-u NILIDFTkKGFLFRG
K777 GQISETLKTRGIFET
T821-p DDSILVKtVCGVVsR
S827-p KtVCGVVsRRAAQLC
R828 tVCGVVsRRAAQLCG
R828 tVCGVVsRRAAQLCG
K844 GMAAVVDKIRENRGL
K844 GMAAVVDKIRENRGL
K880 RIMHQTVKELSPKCN
K885 TVKELSPKCNVSFLL
  rat

 
- gap
Y25-p QVKKIDKyLyAMRLS
Y27-p KKIDKyLyAMRLSDE
K62 YNPTASVKMLPTFVR
K77 SIPDGSEKGDFIALD
S88 IALDLGGSSFRILRV
S89 ALDLGGSSFRILRVQ
K147 KKKIKDKKLPVGFTF
K187 VEGADVVKLLNKAIK
K191 DVVKLLNKAIKKRGD
S298 QLFEKMVSGMYMGEL
K315 LILVKMAKEGLLFEG
K333 PELLTRGKFNTSDVS
S337 TRGKFNTSDVSAIEK
S340 KFNTSDVSAIEKDKE
K346 VSAIEKDKEGIQNAK
K418 GVDGSLYKMHPQYSR
S447 VRFLLSESGTGKGAA
K451 LSESGTGKGAAMVTA
Y461-p AMVTAVAyRLAEQHR
A475 RQIEETLAHFRLSKQ
K488 KQTLMEVKKRLRTEM
T493 EVKKRLRTEMEMGLR
K510 TNSKATVKMLPSFVR
K624 ISWTKGFKATDCEGH
Y732 FDKVVDEYSLNSGKQ
Y749-p EKMISGMyLGEIVRN
K763 NILIDFTKKGFLFRG
K763 NILIDFTKKGFLFRG
K777 GQISEPLKTRGIFET
T821 DDSILVKTVCGVVSK
S827 KTVCGVVSKRAAQLC
K828 TVCGVVSKRAAQLCG
K828 TVCGVVSKRAAQLCG
K844 GMAAVVEKIRENRGL
K844 GMAAVVEKIRENRGL
K880 RIMHQTVKELSPKCT
K885 TVKELSPKCTVSFLL
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