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Protein Page:
HK1 (mouse)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
HK1 a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). HK-2 and its mitochondrial receptor (VDAC) play the most pivotal and direct roles in the ""Warburg effect"". Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Four human isoforms are produced by alternative splicing and alternative initiation. Isoform HK1 is markedly elevated in rapidly growing tumor cells exhibiting high glucose catabolic rates. HK1 is present in most tissues but is especially prominent in brain and kidney. Isoform HK1-SC is is an integral membrane protein detected in round spermatids, condensing spermatids and mature sperm where it is found in the head membranes, mitochondria of the midpiece and the fibrous sheath of the flagellum. Isoform HK1-SA is first expressed during meiosis and continues to be present in postmeiotic germ cells while isoform HK1-SB is present only in postmeiotic germ cells. Note: This description may include information from UniProtKB.
Protein type: Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Mitochondrial; Kinase, other; EC 2.7.1.1; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose
Cellular Component: mitochondrial outer membrane; protein complex; membrane; mitochondrion; cell; caveola; cytosol; cilium; lipid raft
Molecular Function: hexokinase activity; protein homodimerization activity; nucleotide binding; glucokinase activity; protein kinase activity; transferase activity; protein binding; mannokinase activity; fructokinase activity; protein complex binding; kinase activity; phosphotransferase activity, alcohol group as acceptor; catalytic activity; ATP binding; glucose binding
Biological Process: glycolysis; peptidyl-tyrosine phosphorylation; metabolic process; protein amino acid autophosphorylation; regulation of anion channel activity; glucose 6-phosphate metabolic process; peptidyl-threonine phosphorylation; protein amino acid phosphorylation; peptidyl-serine phosphorylation; carbohydrate metabolic process; carbohydrate phosphorylation; cell glucose homeostasis; phosphorylation; negative regulation of apoptosis
Reference #:  P17710 (UniProtKB)
Alt. Names/Synonyms: BB404130; dea; downeast anemia; hexokinase 1; Hexokinase type I; Hexokinase, tumor isozyme; Hexokinase-1; HK I; Hk-1; Hk1; Hk1-s; HXK1; mHk1-s
Gene Symbols: Hk1
Molecular weight: 108,303 Da
Basal Isoelectric point: 6.37  Predict pI for various phosphorylation states
CST Pathways:  Warburg Effect
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

HK1

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

► Hide Isoforms
 
0 1 - gap
0 2 Y81 TEEKIDKYLyAMRLS
0 78 Y83-p EKIDKYLyAMRLSDE
0 1 K118-ub YNPTASVkMLPTFVR
0 1 K133 SIPDGSEKGDFIALD
0 1 K133-ub SIPDGSEkGDFIALD
0 1 S144-p IALDLGGssFRILRV
0 1 S145-p ALDLGGssFRILRVQ
0 1 K203-ub KRKIKDKkLPVGFTF
0 2 S211 LPVGFTFSFPCRQSK
0 1 K229 AVLITWTKRFKASGV
0 1 K232 ITWTKRFKASGVEGA
0 2 K243-ub VEGADVVkLLNKAIK
0 1 K247 DVVkLLNKAIKKRGD
0 1 K346 RGSLNPGKQLFEKMV
0 1 S354 QLFEKMVSGMYMGEL
0 2 Y357 EKMVSGMYMGELVRL
0 1 K371-ub LILVKMAkESLLFEG
0 4 K389-ub PELLTRGkFTTsDVA
0 2 S393-p TRGkFTTsDVAAIET
0 1 A396 kFTTsDVAAIETDkE
0 1 T400 sDVAAIETDkEGVQN
0 3 K402-ub VAAIETDkEGVQNAK
0 1 Y473 VGVDGSLYkMHPQYS
0 2 K474-ub GVDGSLYkMHPQYSR
0 1 M475 VDGSLYkMHPQYSRR
0 1 S501-p SDVRFLLsEsGsGkG
0 2 S503-p VRFLLsEsGsGkGAA
0 1 S505-p FLLsEsGsGkGAAMV
0 1 K507-ub LsEsGsGkGAAMVTA
0 114 Y517-p AMVTAVAyRLAEQHR
0 1 S531-p RQIEETLsHFRLSKQ
0 1 K544-ub KQALMEVkKKLRsEM
0 1 S549-p EVkKKLRsEMEMGLR
0 1 K557 EMEMGLRKETNSRAT
0 1 T559 EMGLRKETNSRATVk
0 2 K566-ub TNSRATVkMLPSYVR
0 1 K600 NFRVLLVKIRSGKKR
0 1 K680-ub ITWTKGFkATDCVGH
0 35 Y788 FDKVVDEYSLNSGKQ
0 10 Y805 EKMISGMYLGEIVRN
0 10 K819-ac NILIDFTkKGFLFRG
0 2 K819-ub NILIDFTkKGFLFRG
0 1 K819 NILIDFTKKGFLFRG
0 1 K833-ub GQISEPLkTRGIFET
0 1 T877 DDSILVKTVCGVVSk
0 1 S883 KTVCGVVSkRAAQLC
0 2 K884-ac TVCGVVSkRAAQLCG
0 8 K884-ub TVCGVVSkRAAQLCG
0 3 K900-ac GMAAVVEkIRENRGL
0 1 K900-ub GMAAVVEkIRENRGL
0 1 T913-p GLDHLNVtVGVDGTL
0 1 K936-ac RIMHQTVkELSPkCT
0 1 K941-ub TVkELSPkCTVSFLL
0 1 S949 CTVSFLLSEDGSGKG
0 1 G969 AVGVRLRGDPTNA__
  HK1 iso3  
S8-p MDSEHCPsVPCGGAG
Y24 WEARIDKYLYAMRLS
Y26 ARIDKYLYAMRLSDE
K61 YNPTASVKMLPTFVR
K76 SIPDGSEKGDFIALD
K76 SIPDGSEKGDFIALD
S87 IALDLGGSSFRILRV
S88 ALDLGGSSFRILRVQ
K146 KRKIKDKKLPVGFTF
S154 LPVGFTFSFPCRQSK
K172 AVLITWTKRFKASGV
K175 ITWTKRFKASGVEGA
K186 VEGADVVKLLNKAIK
K190 DVVKLLNKAIKKRGD
K289 RGSLNPGKQLFEKMV
S297 QLFEKMVSGMYMGEL
Y300 EKMVSGMYMGELVRL
K314 LILVKMAKESLLFEG
K332 PELLTRGKFTTSDVA
S336 TRGKFTTSDVAAIET
A339 KFTTSDVAAIETDKE
T343 SDVAAIETDKEGVQN
K345 VAAIETDKEGVQNAK
Y416 VGVDGSLYKMHPQYS
K417 GVDGSLYKMHPQYSR
M418 VDGSLYKMHPQYSRR
S444 SDVRFLLSESGSGKG
S446 VRFLLSESGSGKGAA
S448 FLLSESGSGKGAAMV
K450 LSESGSGKGAAMVTA
Y460 AMVTAVAYRLAEQHR
S474 RQIEETLSHFRLSKQ
K487 KQALMEVKKKLRSEM
S492 EVKKKLRSEMEMGLR
K500 EMEMGLRKETNSRAT
T502 EMGLRKETNSRATVK
K509 TNSRATVKMLPSYVR
K543 NFRVLLVKIRSGKKR
K623 ITWTKGFKATDCVGH
Y731 FDKVVDEYSLNSGKQ
Y748 EKMISGMYLGEIVRN
K762 NILIDFTKKGFLFRG
K762 NILIDFTKKGFLFRG
K762 NILIDFTKKGFLFRG
K776 GQISEPLKTRGIFET
T820 DDSILVKTVCGVVSK
S826 KTVCGVVSKRAAQLC
K827 TVCGVVSKRAAQLCG
K827 TVCGVVSKRAAQLCG
K843 GMAAVVEKIRENRGL
K843 GMAAVVEKIRENRGL
T856 GLDHLNVTVGVDGTL
K879 RIMHQTVKELSPKCT
K884 TVKELSPKCTVSFLL
S892 CTVSFLLSEDGSGKG
G912 AVGVRLRGDPTNA__
  human

 
- gap
Y25-p QVKKIDKyLyAMRLS
Y27-p KKIDKyLyAMRLSDE
K62 FNPTATVKMLPTFVR
K77-ac SIPDGSEkGDFIALD
K77 SIPDGSEKGDFIALD
S88 IALDLGGSSFRILRV
S89 ALDLGGSSFRILRVQ
K147 KRKIKDKKLPVGFTF
S155-p LPVGFTFsFPCQQSK
K173-ub AILITWTkRFkASGV
K176-ub ITWTkRFkASGVEGA
K187-ub VEGADVVkLLNkAIK
K191-ub DVVkLLNkAIKKRGD
K290-ub RGSLNPGkQLFEKMV
S298-p QLFEKMVsGMyLGEL
Y301-p EKMVsGMyLGELVRL
K315 LILVKMAKEGLLFEG
K333-ub PELLTRGkFNTsDVs
S337-p TRGkFNTsDVsAIEk
S340-p kFNTsDVsAIEkNkE
K344-sc sDVsAIEkNkEGLHN
K346-ub VsAIEkNkEGLHNAK
Y417-p VGVDGSLyktHPQYS
K418-ub GVDGSLyktHPQYSR
T419-p VDGSLyktHPQYSRR
S445 SDVRFLLSESGSGKG
S447 VRFLLSESGSGKGAA
S449 FLLSESGSGKGAAMV
K451 LSESGSGKGAAMVTA
Y461-p AMVTAVAyRLAEQHR
A475 RQIEETLAHFHLTKD
K488 KDMLLEVKKRMRAEM
A493 EVKKRMRAEMELGLR
K501-ub EMELGLRkQtHNNAV
T503-p ELGLRkQtHNNAVVk
K510-ub tHNNAVVkMLPSFVR
K544-ac NFRVLLVkIRSGKKR
K624 ITWTKGFKATDCVGH
Y732-p YDRLVDEySLNAGKQ
Y749-p EKMISGMyLGEIVRN
K763-ac NILIDFTkKGFLFRG
K763-ub NILIDFTkKGFLFRG
K763-sc NILIDFTkKGFLFRG
K777 GQISETLKTRGIFET
T821-p DDSILVKtVCGVVsR
S827-p KtVCGVVsRRAAQLC
R828 tVCGVVsRRAAQLCG
R828 tVCGVVsRRAAQLCG
K844 GMAAVVDKIRENRGL
K844 GMAAVVDKIRENRGL
T857 GLDRLNVTVGVDGTL
K880 RIMHQTVKELSPKCN
K885 TVKELSPKCNVSFLL
S893-p CNVSFLLsEDGSGKG
T913-p AVGVRLRtEASS___
  rat

 
- gap
Y25-p QVKKIDKyLyAMRLS
Y27-p KKIDKyLyAMRLSDE
K62 YNPTASVKMLPTFVR
K77-ac SIPDGSEkGDFIALD
K77 SIPDGSEKGDFIALD
S88 IALDLGGSSFRILRV
S89 ALDLGGSSFRILRVQ
K147 KKKIKDKKLPVGFTF
S155 LPVGFTFSFPCRQSK
K173 AVLITWTKRFKASGV
K176 ITWTKRFKASGVEGA
K187 VEGADVVKLLNKAIK
K191 DVVKLLNKAIKKRGD
K290 RGSLNPGKQLFEKMV
S298 QLFEKMVSGMYMGEL
Y301 EKMVSGMYMGELVRL
K315 LILVKMAKEGLLFEG
K333 PELLTRGKFNTsDVS
S337-p TRGKFNTsDVSAIEK
S340 KFNTsDVSAIEKDKE
K344 sDVSAIEKDKEGIQN
K346 VSAIEKDKEGIQNAK
Y417 VGVDGSLYKMHPQYS
K418 GVDGSLYKMHPQYSR
M419 VDGSLYKMHPQYSRR
S445 SDVRFLLSESGTGKG
S447 VRFLLSESGTGKGAA
T449 FLLSESGTGKGAAMV
K451 LSESGTGKGAAMVTA
Y461-p AMVTAVAyRLAEQHR
A475 RQIEETLAHFRLSKQ
K488 KQTLMEVKKRLRTEM
T493 EVKKRLRTEMEMGLR
K501 EMEMGLRKETNSKAT
T503 EMGLRKETNSKATVK
K510 TNSKATVKMLPSFVR
K544-ac NFRVLLVkIRSGKKR
K624 ISWTKGFKATDCEGH
Y732 FDKVVDEYSLNSGKQ
Y749-p EKMISGMyLGEIVRN
K763 NILIDFTKKGFLFRG
K763 NILIDFTKKGFLFRG
K763 NILIDFTKKGFLFRG
K777 GQISEPLKTRGIFET
T821 DDSILVKTVCGVVSK
S827 KTVCGVVSKRAAQLC
K828 TVCGVVSKRAAQLCG
K828 TVCGVVSKRAAQLCG
K844 GMAAVVEKIRENRGL
K844 GMAAVVEKIRENRGL
T857 GLDHLNVTVGVDGTL
K880-ac RIMHQTVkELSPKCT
K885 TVkELSPKCTVSFLL
S893 CTVSFLLSEDGSGKG
G913 AVGVRLRGDPSIA__
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