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Protein Page:
HK1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
HK1 a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). HK-2 and its mitochondrial receptor (VDAC) play the most pivotal and direct roles in the ""Warburg effect"". Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Four human isoforms are produced by alternative splicing and alternative initiation. Isoform HK1 is markedly elevated in rapidly growing tumor cells exhibiting high glucose catabolic rates. HK1 is present in most tissues but is especially prominent in brain and kidney. Isoform HK1-SC is is an integral membrane protein detected in round spermatids, condensing spermatids and mature sperm where it is found in the head membranes, mitochondria of the midpiece and the fibrous sheath of the flagellum. Isoform HK1-SA is first expressed during meiosis and continues to be present in postmeiotic germ cells while isoform HK1-SB is present only in postmeiotic germ cells. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.1.1; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose; Kinase, other; Mitochondrial
Chromosomal Location of Human Ortholog: 10q22
Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol; lipid raft
Molecular Function: hexokinase activity; protein binding; mannokinase activity; fructokinase activity; glucokinase activity; ATP binding
Biological Process: cell death; hexose metabolic process; glycolysis; hexose transport; carbohydrate metabolic process; carbohydrate phosphorylation; glucose 6-phosphate metabolic process; cell glucose homeostasis; glucose transport; transmembrane transport
Reference #:  P19367 (UniProtKB)
Alt. Names/Synonyms: brain form hexokinase; glycolytic enzyme; hexokinase 1; Hexokinase 1 isoform HKI variant; hexokinase type I; hexokinase-1; HK I; HK1; HK1-ta; HK1-tb; HK1-tc; HKI; HXK1
Gene Symbols: HK1
Molecular weight: 102,486 Da
Basal Isoelectric point: 6.36  Predict pI for various phosphorylation states
CST Pathways:  Warburg Effect
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

HK1

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 - gap
0 2 Y25-p QVKKIDKyLyAMRLS
0 78 Y27-p KKIDKyLyAMRLSDE
0 1 K62 FNPTATVKMLPTFVR
0 1 K77-ac SIPDGSEkGDFIALD
0 1 K77 SIPDGSEKGDFIALD
0 1 S88 IALDLGGSSFRILRV
0 1 S89 ALDLGGSSFRILRVQ
0 1 K147 KRKIKDKKLPVGFTF
0 2 S155-p LPVGFTFsFPCQQSK
0 1 K173-ub AILITWTkRFkASGV
0 1 K176-ub ITWTkRFkASGVEGA
0 2 K187-ub VEGADVVkLLNkAIK
0 1 K191-ub DVVkLLNkAIKKRGD
0 1 K290-ub RGSLNPGkQLFEKMV
0 1 S298-p QLFEKMVsGMyLGEL
0 2 Y301-p EKMVsGMyLGELVRL
0 1 K315 LILVKMAKEGLLFEG
0 4 K333-ub PELLTRGkFNTsDVs
0 2 S337-p TRGkFNTsDVsAIEk
0 1 S340-p kFNTsDVsAIEkNkE
0 1 K344-sc sDVsAIEkNkEGLHN
0 3 K346-ub VsAIEkNkEGLHNAK
0 1 Y417-p VGVDGSLyktHPQYS
0 2 K418-ub GVDGSLyktHPQYSR
0 1 T419-p VDGSLyktHPQYSRR
0 1 S445 SDVRFLLSESGSGKG
0 2 S447 VRFLLSESGSGKGAA
0 1 S449 FLLSESGSGKGAAMV
0 1 K451 LSESGSGKGAAMVTA
0 114 Y461-p AMVTAVAyRLAEQHR
0 1 A475 RQIEETLAHFHLTKD
0 1 K488 KDMLLEVKKRMRAEM
0 1 A493 EVKKRMRAEMELGLR
0 1 K501-ub EMELGLRkQtHNNAV
0 1 T503-p ELGLRkQtHNNAVVk
0 2 K510-ub tHNNAVVkMLPSFVR
0 1 K544-ac NFRVLLVkIRSGKKR
0 1 K624 ITWTKGFKATDCVGH
0 35 Y732-p YDRLVDEySLNAGKQ
0 10 Y749-p EKMISGMyLGEIVRN
0 10 K763-ac NILIDFTkKGFLFRG
0 2 K763-ub NILIDFTkKGFLFRG
0 1 K763-sc NILIDFTkKGFLFRG
0 1 K777 GQISETLKTRGIFET
0 1 T821-p DDSILVKtVCGVVsR
0 1 S827-p KtVCGVVsRRAAQLC
0 2 R828 tVCGVVsRRAAQLCG
0 8 R828 tVCGVVsRRAAQLCG
0 3 K844 GMAAVVDKIRENRGL
0 1 K844 GMAAVVDKIRENRGL
0 1 T857 GLDRLNVTVGVDGTL
0 1 K880 RIMHQTVKELSPKCN
0 1 K885 TVKELSPKCNVSFLL
0 1 S893-p CNVSFLLsEDGSGKG
0 1 T913-p AVGVRLRtEASS___
  mouse

► Hide Isoforms
 
- gap
Y81 TEEKIDKYLyAMRLS
Y83-p EKIDKYLyAMRLSDE
K118-ub YNPTASVkMLPTFVR
K133 SIPDGSEKGDFIALD
K133-ub SIPDGSEkGDFIALD
S144-p IALDLGGssFRILRV
S145-p ALDLGGssFRILRVQ
K203-ub KRKIKDKkLPVGFTF
S211 LPVGFTFSFPCRQSK
K229 AVLITWTKRFKASGV
K232 ITWTKRFKASGVEGA
K243-ub VEGADVVkLLNKAIK
K247 DVVkLLNKAIKKRGD
K346 RGSLNPGKQLFEKMV
S354 QLFEKMVSGMYMGEL
Y357 EKMVSGMYMGELVRL
K371-ub LILVKMAkESLLFEG
K389-ub PELLTRGkFTTsDVA
S393-p TRGkFTTsDVAAIET
A396 kFTTsDVAAIETDkE
T400 sDVAAIETDkEGVQN
K402-ub VAAIETDkEGVQNAK
Y473 VGVDGSLYkMHPQYS
K474-ub GVDGSLYkMHPQYSR
M475 VDGSLYkMHPQYSRR
S501-p SDVRFLLsEsGsGkG
S503-p VRFLLsEsGsGkGAA
S505-p FLLsEsGsGkGAAMV
K507-ub LsEsGsGkGAAMVTA
Y517-p AMVTAVAyRLAEQHR
S531-p RQIEETLsHFRLSKQ
K544-ub KQALMEVkKKLRsEM
S549-p EVkKKLRsEMEMGLR
K557 EMEMGLRKETNSRAT
T559 EMGLRKETNSRATVk
K566-ub TNSRATVkMLPSYVR
K600 NFRVLLVKIRSGKKR
K680-ub ITWTKGFkATDCVGH
Y788 FDKVVDEYSLNSGKQ
Y805 EKMISGMYLGEIVRN
K819-ac NILIDFTkKGFLFRG
K819-ub NILIDFTkKGFLFRG
K819 NILIDFTKKGFLFRG
K833-ub GQISEPLkTRGIFET
T877 DDSILVKTVCGVVSk
S883 KTVCGVVSkRAAQLC
K884-ac TVCGVVSkRAAQLCG
K884-ub TVCGVVSkRAAQLCG
K900-ac GMAAVVEkIRENRGL
K900-ub GMAAVVEkIRENRGL
T913-p GLDHLNVtVGVDGTL
K936-ac RIMHQTVkELSPkCT
K941-ub TVkELSPkCTVSFLL
S949 CTVSFLLSEDGSGKG
G969 AVGVRLRGDPTNA__
  HK1 iso3  
S8-p MDSEHCPsVPCGGAG
Y24 WEARIDKYLYAMRLS
Y26 ARIDKYLYAMRLSDE
K61 YNPTASVKMLPTFVR
K76 SIPDGSEKGDFIALD
K76 SIPDGSEKGDFIALD
S87 IALDLGGSSFRILRV
S88 ALDLGGSSFRILRVQ
K146 KRKIKDKKLPVGFTF
S154 LPVGFTFSFPCRQSK
K172 AVLITWTKRFKASGV
K175 ITWTKRFKASGVEGA
K186 VEGADVVKLLNKAIK
K190 DVVKLLNKAIKKRGD
K289 RGSLNPGKQLFEKMV
S297 QLFEKMVSGMYMGEL
Y300 EKMVSGMYMGELVRL
K314 LILVKMAKESLLFEG
K332 PELLTRGKFTTSDVA
S336 TRGKFTTSDVAAIET
A339 KFTTSDVAAIETDKE
T343 SDVAAIETDKEGVQN
K345 VAAIETDKEGVQNAK
Y416 VGVDGSLYKMHPQYS
K417 GVDGSLYKMHPQYSR
M418 VDGSLYKMHPQYSRR
S444 SDVRFLLSESGSGKG
S446 VRFLLSESGSGKGAA
S448 FLLSESGSGKGAAMV
K450 LSESGSGKGAAMVTA
Y460 AMVTAVAYRLAEQHR
S474 RQIEETLSHFRLSKQ
K487 KQALMEVKKKLRSEM
S492 EVKKKLRSEMEMGLR
K500 EMEMGLRKETNSRAT
T502 EMGLRKETNSRATVK
K509 TNSRATVKMLPSYVR
K543 NFRVLLVKIRSGKKR
K623 ITWTKGFKATDCVGH
Y731 FDKVVDEYSLNSGKQ
Y748 EKMISGMYLGEIVRN
K762 NILIDFTKKGFLFRG
K762 NILIDFTKKGFLFRG
K762 NILIDFTKKGFLFRG
K776 GQISEPLKTRGIFET
T820 DDSILVKTVCGVVSK
S826 KTVCGVVSKRAAQLC
K827 TVCGVVSKRAAQLCG
K827 TVCGVVSKRAAQLCG
K843 GMAAVVEKIRENRGL
K843 GMAAVVEKIRENRGL
T856 GLDHLNVTVGVDGTL
K879 RIMHQTVKELSPKCT
K884 TVKELSPKCTVSFLL
S892 CTVSFLLSEDGSGKG
G912 AVGVRLRGDPTNA__
  rat

 
- gap
Y25-p QVKKIDKyLyAMRLS
Y27-p KKIDKyLyAMRLSDE
K62 YNPTASVKMLPTFVR
K77-ac SIPDGSEkGDFIALD
K77 SIPDGSEKGDFIALD
S88 IALDLGGSSFRILRV
S89 ALDLGGSSFRILRVQ
K147 KKKIKDKKLPVGFTF
S155 LPVGFTFSFPCRQSK
K173 AVLITWTKRFKASGV
K176 ITWTKRFKASGVEGA
K187 VEGADVVKLLNKAIK
K191 DVVKLLNKAIKKRGD
K290 RGSLNPGKQLFEKMV
S298 QLFEKMVSGMYMGEL
Y301 EKMVSGMYMGELVRL
K315 LILVKMAKEGLLFEG
K333 PELLTRGKFNTsDVS
S337-p TRGKFNTsDVSAIEK
S340 KFNTsDVSAIEKDKE
K344 sDVSAIEKDKEGIQN
K346 VSAIEKDKEGIQNAK
Y417 VGVDGSLYKMHPQYS
K418 GVDGSLYKMHPQYSR
M419 VDGSLYKMHPQYSRR
S445 SDVRFLLSESGTGKG
S447 VRFLLSESGTGKGAA
T449 FLLSESGTGKGAAMV
K451 LSESGTGKGAAMVTA
Y461-p AMVTAVAyRLAEQHR
A475 RQIEETLAHFRLSKQ
K488 KQTLMEVKKRLRTEM
T493 EVKKRLRTEMEMGLR
K501 EMEMGLRKETNSKAT
T503 EMGLRKETNSKATVK
K510 TNSKATVKMLPSFVR
K544-ac NFRVLLVkIRSGKKR
K624 ISWTKGFKATDCEGH
Y732 FDKVVDEYSLNSGKQ
Y749-p EKMISGMyLGEIVRN
K763 NILIDFTKKGFLFRG
K763 NILIDFTKKGFLFRG
K763 NILIDFTKKGFLFRG
K777 GQISEPLKTRGIFET
T821 DDSILVKTVCGVVSK
S827 KTVCGVVSKRAAQLC
K828 TVCGVVSKRAAQLCG
K828 TVCGVVSKRAAQLCG
K844 GMAAVVEKIRENRGL
K844 GMAAVVEKIRENRGL
T857 GLDHLNVTVGVDGTL
K880-ac RIMHQTVkELSPKCT
K885 TVkELSPKCTVSFLL
S893 CTVSFLLSEDGSGKG
G913 AVGVRLRGDPSIA__
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