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Protein Page:
E2F1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
E2F1 a member of the E2F/DP family of transcription factors.The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. Binds DNA cooperatively with Dp transcription factors in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.The Dp-1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. It can mediate both cell proliferation and p53-dependent apoptosis. Note: This description may include information from UniProtKB.
Protein type: Transcription factor; DNA binding protein
Cellular Component: nucleoplasm; Rb-E2F complex; cytoplasm; nucleus
Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor binding; transcription factor activity; transcription corepressor activity
Biological Process: Notch signaling pathway; transcription, DNA-dependent; apoptosis; positive regulation of transcription, DNA-dependent; mRNA stabilization; negative regulation of transcription from RNA polymerase II promoter; G2 phase of mitotic cell cycle; anoikis; cell proliferation; positive regulation of fibroblast proliferation; DNA damage response, signal transduction resulting in induction of apoptosis; regulation of transcription, DNA-dependent; DNA damage checkpoint; forebrain development; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; G1 phase of mitotic cell cycle; G1/S transition of mitotic cell cycle
Reference #:  Q01094 (UniProtKB)
Alt. Names/Synonyms: E2F transcription factor 1; E2F-1; E2F1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBAP1; RBBP-3; RBBP3; RBP3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1
Gene Symbols: E2F1
Molecular weight: 46,920 Da
Basal Isoelectric point: 4.79  Predict pI for various phosphorylation states
CST Pathways:  ErbB/HER Signaling  |  G1/S Checkpoint
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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E2F1

Protein Structure Not Found.


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Sites Implicated In
apoptosis, induced: S364‑p
cell cycle regulation: S332‑p, S337‑p
cell differentiation, altered: S403‑p, T433‑p
transcription, altered: S403‑p
transcription, induced: S364‑p
transcription, inhibited: S375‑p
intracellular localization: S364‑p, S403‑p, T433‑p
molecular association, regulation: S332‑p, S337‑p
protein degradation: S403‑p, T433‑p
protein stabilization: S31‑p, S364‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
2 0 S31-p ALRLLDSsQIVIISA
0 1 T96-p KRRLDLEtDHQYLAE
3 0 K117-ac GRGRHPGkGVkSPGE
3 0 K120-ac RHPGkGVkSPGEkSR
3 0 K125-ac GVkSPGEkSRYETSL
0 1 T135-p YETSLNLttKRFLEL
0 1 T136-p ETSLNLttKRFLELL
1 0 K185-m1 QLIAKKSkNHIQWLG
2 0 S332-p TDSATIVsPPPSsPP
2 0 S337-p IVsPPPSsPPSSLTT
3 1 S364-p PLLSRMGsLRAPVDE
2 3 S375-p PVDEDRLsPLVAADS
5 0 S403-p PEEFISLsPPHEALD
3 0 T433-p DCDFGDLtPLDF___
  mouse

 
S29 ALRLLDSSQIVIIST
T91 KRRLDLETDHQYLAG
K112 GRGRHPGKGVKSPGE
K115 RHPGKGVKSPGEKSR
K120 GVKSPGEKSRYETSL
T130 YETSLNLTTKRFLEL
T131 ETSLNLTTKRFLELL
K180 QLIAKKSKNHIQWLG
G325 TADSGPAGPPPSPPS
P330 PAGPPPSPPSTSPAL
H357 AVLPRMGHLRVPMEE
S368 PMEEDQLSPLVAADS
S396 PGEFISLSPPHEALD
T426 DCDFGDLTPLDF___
  rat

 
S29 ALRLLDSSQIVIIST
T94 KRRLDLETDHQYLAG
K115 GRGRHPGKGVKSPGE
K118 RHPGKGVKSPGEKSR
K123 GVKSPGEKSRYETSL
T133 YETSLNLTTKRFLEL
T134 ETSLNLTTKRFLELL
K183 QLIAKKSKNHIQWLG
G327 NADSGTAGPPPSPPS
P332 TAGPPPSPPSTSPTL
N359 AVLPRIGNLRAPMEE
S370 PMEEDRLSPLVAADS
S398 PGEFISLSPPHEAVD
T428 DCDFGDLTPLDF___
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