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Protein Page:
PFKM (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
PFKM phosphofructokinase, muscle type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Defects in PFKM are the cause of glycogen storage disease VII (GSD-VII) also known as Tarui disease. Two alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Kinase, other
Cellular Component: 6-phosphofructokinase complex; apical plasma membrane; cytosol
Molecular Function: protein C-terminus binding; identical protein binding; protein binding; metal ion binding; kinase binding; ATP binding; 6-phosphofructokinase activity; AMP binding
Biological Process: muscle maintenance; fructose 1,6-bisphosphate metabolic process; glycolysis; carbohydrate metabolic process; positive regulation of insulin secretion; glucose metabolic process; glucose homeostasis; fructose 6-phosphate metabolic process; protein oligomerization; protein homotetramerization
Reference #:  NP_001160158 (RefSeq)
Alt. Names/Synonyms: 6-phosphofructo-1-kinase; 6-phosphofructokinase, muscle type; GSD7; K6PF; MGC8699; PFK-1; PFK-A; PFK1; PFKA; PFKM; PFKX; Phosphofructo-1-kinase isozyme A; Phosphofructokinase 1; phosphofructokinase, muscle; phosphofructokinase, polypeptide X; Phosphofructokinase-M; Phosphohexokinase
Gene Symbols: PFKM
Molecular weight: 93,254 Da
Basal Isoelectric point: 8.2  Predict pI for various phosphorylation states
CST Pathways:  Warburg Effect
Select Structure to View Below

PFKM
Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

► Hide Isoforms 		 
0 1 K49-u PPKTDILkSLDTMDD
0 1 S62-p DDPDTVGsIPVFKTE
0 1 K81-a HEEHHAAkTLGIGkA
0 1 K87-u AkTLGIGkAIAVLTS
0 1 T153-p MMLQLGGtVIGSARC
0 136 Y174-p EGRLRAAyNLVKRGI
0 1 K178 RAAyNLVKRGITNLC
0 4 K212-u DLLSDLQkAGkITDE
0 1 K215-u SDLQkAGkITDEEAT
0 1 K343-u GAIDKNGkPITSEDI
0 1 K351 PITSEDIKNLVVKRL
0 2 T375 GHVQRGGTPSAFDRI
0 2 K431-a QVTKDVTkAMDEKkF
0 1 K431-u QVTKDVTkAMDEKkF
0 2 K437-a TkAMDEKkFDEALkL
0 1 K437-u TkAMDEKkFDEALkL
0 1 K443 KkFDEALKLRGRsFM
0 4 K443-u KkFDEALkLRGRsFM
1 7 S448-p ALkLRGRsFMNNWEV
0 1 S469-p VRPPVSKsGsHtVAV
0 1 S471-p PPVSKsGsHtVAVMN
0 1 T473-p VSKsGsHtVAVMNVG
0 1 K516-u DGFEGLAkGQIEEAG
0 1 K537-u WTGQGGSkLGTKRTL
0 1 K547-u TKRTLPKkSFEQISA
0 1 Y573-p IIGGFEAyTGGLELM
0 12 K635-a KQSAAGTkRRVFIIE
0 3 Y647-p IIETMGGyCGYLATM
0 1 K686-u NVEHLVQkMKTTVKR
0 1 K727-u KGIFDSRkNVLGHMQ
0 3 S738-p GHMQQGGsPTPFDRN
0 3 K749-u FDRNFATkMGAkAMN
0 1 K753-u FATkMGAkAMNWMSG
0 1 K798-u FQPVAELkDQTDFEH
0 1 Y826-p ILKILAKyEIDLDTS
2 15 S846-p EHITRKRsGEAAV__
  PFKM iso2  
- gap
- gap
K10 HEEHHAAKTLGIGKA
K16 AKTLGIGKAIAVLTS
T82 MMLQLGGTVIGSARC
Y103 EGRLRAAYNLVKRGI
K107 RAAYNLVKRGITNLC
K141 DLLSDLQKAGKITDE
K144 SDLQKAGKITDEEAT
K272-u GAIDKNGkPITSEDI
K280-u PITSEDIkNGSRMGV
- gap
K329 QVTKDVTKAMDEKKF
K329 QVTKDVTKAMDEKKF
K335 TKAMDEKKFDEALKL
K335 TKAMDEKKFDEALKL
K341 KKFDEALKLRGRSFM
K341 KKFDEALKLRGRSFM
S346 ALKLRGRSFMNNWEV
S367 VRPPVSKSGSHTVAV
S369 PPVSKSGSHTVAVMN
T371 VSKSGSHTVAVMNVG
K414 DGFEGLAKGQIEEAG
K435 WTGQGGSKLGTKRTL
K445 TKRTLPKKSFEQISA
Y471 IIGGFEAYTGGLELM
K533 KQSAAGTKRRVFIIE
Y545 IIETMGGYCGYLATM
K584 NVEHLVQKMKTTVKR
K625 KGIFDSRKNVLGHMQ
S636 GHMQQGGSPTPFDRN
K647 FDRNFATKMGAKAMN
K651 FATKMGAKAMNWMSG
K696 FQPVAELKDQTDFEH
Y724 ILKILAKYEIDLDTS
S744 EHITRKRSGEAAV__
  mouse

 
- gap
- gap
K10 HEEHHAAKTLGIGKA
K16 AKTLGIGKAIAVLTS
T82 MMLQLGGTVIGSARC
H103 EGRLRAAHNLVkRGI
K107-u RAAHNLVkRGITNLC
K141-u DLLNDLQkDGKITAE
K144 NDLQkDGKITAEEAT
K272 GAIDKNGKPITSEDI
K280 PITSEDIKNLVVKRL
T304-p GHVQRGGtPSAFDRI
K360 QVTKDVTKAMDEKRF
K360 QVTKDVTKAMDEKRF
R366 TKAMDEKRFDEAIkL
R366 TKAMDEKRFDEAIkL
K372 KRFDEAIKLRGRsFM
K372-u KRFDEAIkLRGRsFM
S377-p AIkLRGRsFMNNWEV
G398 VRPPVSKGGLHTVAV
L400 PPVSKGGLHTVAVMN
T402 VSKGGLHTVAVMNVG
K445 DGFEGLAKGQIEEAG
K466 WTGQGGSKLGTKRTL
K476 TKRTLPKKNLEQISA
Y502 IIGGFEAYTGGLELM
K564 KQSAAGTKRRVFIIE
Y576 IIETMGGYCGYLATM
K615 NVEHLVQKMKTTVKR
K656 KGIFDSRKNVLGHMQ
S667-p GHMQQGGsPTPFDRN
K678-u FDRNFATkMGAKAMN
K682 FATkMGAKAMNWMSG
K727 FQPVTELKDQTDFEH
Y755 ILKILAKYEIDLDTS
S775-p EHISRKRsGEAAV__
  rat

 
- gap
- gap
K10 HEEHHEAKTLGIGKA
K16 AKTLGIGKAIAVLTS
T82 MMLQLGGTVIGSARC
H103 EGRLRAAHNLVKRGI
K107 RAAHNLVKRGITNLC
K141 DLLNDLQKDGKITAE
K144 NDLQKDGKITAEERT
K272 GAIDKNGKPITSEDI
K280 PITSEDIKNLVVKRL
T304 GHVQRGGTPSAFDRI
K360 QVTKDVTKAMDEKRF
K360 QVTKDVTKAMDEKRF
R366 TKAMDEKRFDEAIkL
R366 TKAMDEKRFDEAIkL
K372-a KRFDEAIkLRGRSFM
K372 KRFDEAIKLRGRSFM
S377 AIkLRGRSFMNNWEV
G398 VRPPVSKGGLHTVAV
L400 PPVSKGGLHTVAVMN
T402 VSKGGLHTVAVMNVG
K445 DGFEGLAKGQIEEAG
K466 WTGQGGSKLGTKRTL
K476 TKRTLPKKNLEQISA
Y502 IIGGFEAYTGGLELM
K564 KQSAAGTKRRVFIIE
Y576 IIETMGGYCGYLATM
K615 NVEHLVQKMKTTVKR
K656 KGIFDSRKNVLGHMQ
N667 GHMQQGGNPTPFDRN
K678 FDRNFATKMGAKATN
K682 FATKMGAKATNWMSG
K727 FQPVTELKDQTDFEH
Y755 ILKILAKYEIDLDTS
S775 EHISRKRSGEAAV__
  rabbit

 
- gap
- gap
R10 HEEHHAARTLGVGKA
K16 ARTLGVGKAIAVLTS
T82 MMLQLGGTVIGSARC
H103 EGRLRAAHNLVKRGI
K107 RAAHNLVKRGITNLC
K141 DLLSDLQKAGKITAE
K144 SDLQKAGKITAEEAT
K272 GAIDRNGKPITSEGV
K280 PITSEGVKDLVVRRL
T304 GHVQRGGTPSAFDRI
K360 QVTKDVTKAMDEKRF
K360 QVTKDVTKAMDEKRF
R366 TKAMDEKRFDEAMKL
R366 TKAMDEKRFDEAMKL
K372 KRFDEAMKLRGRsFM
K372 KRFDEAMKLRGRsFM
S377-p AMKLRGRsFMNNWEV
S398 IRPPAPKSGSYTVAV
S400 PPAPKSGSYTVAVMN
T402 APKSGSYTVAVMNVG
K445 DGFEGPAKGQIEEAG
K466 WTGQGGSKLGSKRTL
K476 SKRTLPKKSFEQISA
Y502 IIGGFEAYTGGLELM
K564 KQSAAGTKRRVFIIE
Y576 IIETMGGYCGYLATM
K615 NVEHLVQKMKTTVKR
K656 KGIFDSRKNVLGHMQ
S667 GHMQQGGSPTPFDRN
K678 FDRNFATKMGAKAMN
K682 FATKMGAKAMNWMAG
Q727 FQPVTELQNQTDFEH
Y755 ILKILAKYEIDLDTS
S775-p EHISRKRsGEATV__
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