Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
Smad3 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton. Note: This description may include information from UniProtKB.
Protein type: Nuclear receptor co-regulator; Transcription factor; DNA binding protein
Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; plasma membrane; nuclear inner membrane; nucleus; cytosol; receptor complex
Molecular Function: ubiquitin binding; protein homodimerization activity; zinc ion binding; chromatin DNA binding; beta-catenin binding; transcription factor binding; protein kinase binding; phosphatase binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; collagen binding; protein binding; sequence-specific DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; bHLH transcription factor binding; transcription factor activity; transforming growth factor beta receptor binding
Biological Process: developmental growth; positive regulation of positive chemotaxis; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; embryonic pattern specification; regulation of transforming growth factor beta receptor signaling pathway; transforming growth factor beta receptor signaling pathway; embryonic foregut morphogenesis; positive regulation of stress fiber formation; negative regulation of mitotic cell cycle; negative regulation of osteoblast differentiation; cell cycle arrest; regulation of striated muscle development; pericardium development; somitogenesis; transcription, DNA-dependent; embryonic cranial skeleton morphogenesis; osteoblast development; positive regulation of chondrocyte differentiation; mesoderm formation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of osteoblast proliferation; negative regulation of protein catabolic process; endoderm development; negative regulation of apoptosis; evasion of host defenses by virus; T cell activation; wound healing; primary microRNA processing; negative regulation of transcription from RNA polymerase II promoter; regulation of epithelial cell proliferation; positive regulation of focal adhesion formation; negative regulation of protein amino acid phosphorylation; ureteric bud development; transport; positive regulation of transforming growth factor-beta3 production; thyroid gland development; positive regulation of transcription factor import into nucleus; heart looping; caspase activation; transcription initiation from RNA polymerase II promoter; intercellular junction assembly and maintenance; regulation of transforming growth factor-beta2 production; regulation of immune response; protein stabilization; regulation of binding; in utero embryonic development; positive regulation of bone mineralization; liver development; SMAD protein complex assembly; positive regulation of interleukin-1 beta production; immune system development; negative regulation of inflammatory response; response to hypoxia; gene expression; immune response; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration
Reference #:  P84022 (UniProtKB)
Alt. Names/Synonyms: DKFZp586N0721; DKFZp686J10186; hMAD-3; hSMAD3; HSPC193; HsT17436; JV15-2; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; Mad3; MADH3; MGC60396; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD 3; SMAD family member 3; SMAD, mothers against DPP homolog 3; SMAD3
Gene Symbols: SMAD3
Molecular weight: 48,081 Da
Basal Isoelectric point: 6.73  Predict pI for various phosphorylation states
CST Pathways:  Angiogenesis  |  ESC Pluripotency and Differentiation  |  G1/S Checkpoint  |  TGF-├č Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Smad3

Protein Structure Not Found.


STRING  |  Wikipedia  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  DISEASE  |  Scansite  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene  |  NURSA  |  InnateDB


Sites Implicated In
cell cycle regulation: T8‑p, T179‑p, S213‑p
cell growth, altered: T179‑p, S204‑p, S208‑p, S213‑p
cell motility, altered: S423‑p, S425‑p
signaling pathway regulation: S204‑p
transcription, altered: T8‑p, T179‑p, S204‑p, S208‑p, S213‑p, S422‑p, S423‑p, S425‑p
transcription, induced: T388‑p
transcription, inhibited: T8‑p, T179‑p, S204‑p, S208‑p, S213‑p, T388‑p
activity, induced: S423‑p, S425‑p
activity, inhibited: T179‑p, S204‑p, S208‑p
intracellular localization: T179‑p, S204‑p, S208‑p, S213‑p, S416‑p, S418‑p
molecular association, regulation: T179‑p, S204‑p, T388‑p
protein conformation: S423‑p, S425‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
5 4 T8-p MSSILPFtPPIVKRL
0 5 K19-ac VKRLLGWkKGEQNGQ
0 1 K29-ac EQNGQEEkWCEKAVK
0 3 T132-p YHYQRVEtPVLPPVL
10 0 T179-p PQSNIPEtPPPGYLS
11 0 S204-p NHSMDAGsPNLsPNP
11 0 S208-p DAGsPNLsPNPMsPA
8 0 S213-p NLsPNPMsPAHNNLD
2 0 S309-p EVFAECLsDSAIFVQ
1 2 K378-ac TIRMSFVkGWGAEYR
3 0 T388-p GAEYRRQtVTSTPCW
1 3 S416-p KVLTQMGsPsIRCss
2 0 S418-p LTQMGsPsIRCssVs
2 0 S422-p GsPsIRCssVs____
18 5 S423-p sPsIRCssVs_____
19 5 S425-p sIRCssVs_______
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
  mouse

 
T8 MSSILPFTPPIVKRL
K19 VKRLLGWKKGEQNGQ
K29 EQNGQEEKWCEKAVK
T132-p YHYQRVEtPVLPPVL
T179 PQSNIPETPPPGYLS
S204 NHSMDAGSPNLSPNP
S208 DAGSPNLSPNPMSPA
S213 NLSPNPMSPAHNNLD
S309-p EVFAECLsDSAIFVQ
K378 TIRMSFVKGWGAEYR
T388-p GAEYRRQtVTSTPCW
S416 KVLTQMGSPSIRCSs
S418 LTQMGSPSIRCSsVs
S422 GSPSIRCSsVs____
S423-p SPSIRCSsVs_____
S425-p SIRCSsVs_______
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
  rat

 
T8-p MSSILPFtPPIVKRL
K19 VKRLLGWKKGEQNGQ
K29 EQNGQEEKWCEKAVK
T132 YHYQRVETPVLPPVL
T179 PQSNIPETPPPGYLS
S204 NHSMDAGSPNLSPNP
S208 DAGSPNLSPNPMSPA
S213 NLSPNPMSPAHNNLD
S309 EVFAECLSDSAIFVQ
K378 TIRMSFVKGWGAEYR
T388 GAEYRRQTVTSTPCW
S416 KVLTQMGSPSIRCSs
S418 LTQMGSPSIRCSsVs
S422 GSPSIRCSsVs____
S423-p SPSIRCSsVs_____
S425-p SIRCSsVs_______
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.