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Smad3 (human)

Overview Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton. Note: This description may include information from UniProtKB. Protein type: DNA binding protein; Nuclear receptor co-regulator; Transcription factor Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; plasma membrane; nuclear inner membrane; cytosol; nucleus; receptor complex Molecular Function: identical protein binding; ubiquitin binding; protein homodimerization activity; zinc ion binding; chromatin DNA binding; beta-catenin binding; protein kinase binding; transcription factor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; phosphatase binding; collagen binding; protein binding; sequence-specific DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; transforming growth factor beta receptor binding; transcription factor activity Biological Process: developmental growth; positive regulation of positive chemotaxis; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; embryonic pattern specification; regulation of transforming growth factor beta receptor signaling pathway; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; embryonic foregut morphogenesis; negative regulation of osteoblast differentiation; negative regulation of mitotic cell cycle; cell cycle arrest; regulation of striated muscle development; pericardium development; somitogenesis; release of cytochrome c from mitochondria; transcription, DNA-dependent; embryonic cranial skeleton morphogenesis; osteoblast development; positive regulation of chondrocyte differentiation; mesoderm formation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of osteoblast proliferation; negative regulation of protein catabolic process; negative regulation of apoptosis; endoderm development; evasion of host defenses by virus; wound healing; T cell activation; negative regulation of transcription from RNA polymerase II promoter; primary microRNA processing; regulation of epithelial cell proliferation; negative regulation of protein amino acid phosphorylation; positive regulation of focal adhesion formation; transport; ureteric bud development; positive regulation of transforming growth factor-beta3 production; thyroid gland development; positive regulation of transcription factor import into nucleus; heart looping; caspase activation; intercellular junction assembly and maintenance; transcription initiation from RNA polymerase II promoter; regulation of immune response; regulation of transforming growth factor-beta2 production; regulation of binding; protein stabilization; in utero embryonic development; liver development; positive regulation of bone mineralization; SMAD protein complex assembly; positive regulation of interleukin-1 beta production; immune system development; negative regulation of inflammatory response; induction of apoptosis; response to hypoxia; gene expression; immune response; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration Reference #:  P84022 (UniProtKB) Alt. Names/Synonyms: DKFZp586N0721; DKFZp686J10186; hMAD-3; hSMAD3; HSPC193; HsT17436; JV15-2; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; Mad3; MADH3; MGC60396; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD 3; SMAD family member 3; SMAD, mothers against DPP homolog 3; SMAD3 Gene Symbols: SMAD3 Molecular weight: 48,081 Da Basal Isoelectric point: 6.73  Predict pI for various phosphorylation states CST Pathways:  Angiogenesis  |  Cell Cycle: G1/S Checkpoint  |  ESC Pluripotency and Differentiation  |  TGF-ß Signaling Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below Smad3 1MHD - A/B=1-132 (human) 1MJS - A=229-425 (human) 1MK2 - A=220-425 (human) 1OZJ - A/B=1-144 (human) 1U7F - A/C=228-424 (human) 2LAJ - B=202-211 (human) 2LB2 - B=178-189 (human)

STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 1MHD 1MJS 1MK2 1OZJ 1U7F 2LAJ 2LB2  |  Phospho3D  |  DISEASE  |  Source  |  NURSA  |  InnateDB  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene

	Sites Implicated In
cell cycle regulation: T8‑p, T179‑p, S213‑p cell growth, altered: T179‑p, S204‑p, S208‑p, S213‑p cell motility, altered: S423‑p, S425‑p signaling pathway regulation: S204‑p transcription, altered: T8‑p, T179‑p, S204‑p, S208‑p, S213‑p, S422‑p, S423‑p, S425‑p transcription, inhibited: T388‑p activation: S423‑p, S425‑p inhibition: T179‑p, S204‑p, S208‑p intracellular localization: T179‑p, S204‑p, S208‑p, S213‑p, S416‑p, S418‑p molecular association, regulation: T179‑p, S204‑p, T388‑p protein conformation: S423‑p, S425‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
5	1		T8-p	MSSILPFtPPIVKRL
0	5		K19-a	VKRLLGWkKGEQNGQ
0	1		K29-a	EQNGQEEkWCEKAVK
0	1		Y125-p	DEVCVNPyHYQRVEt
0	2		T132-p	yHYQRVEtPVLPPVL
10	0		T179-p	PQSNIPEtPPPGYLS
11	0		S204-p	NHSMDAGsPNLsPNP
11	0		S208-p	DAGsPNLsPNPMsPA
8	0		S213-p	NLsPNPMsPAHNNLD
2	0		S309-p	EVFAECLsDSAIFVQ
1	1		K378-a	TIRMSFVkGWGAEYR
2	0		T388-p	GAEYRRQtVTSTPCW
1	1		S416-p	KVLTQMGsPsIRCss
2	0		S418-p	LTQMGsPsIRCssVs
1	0		S422-p	GsPsIRCssVs____
16	5		S423-p	sPsIRCssVs_____
16	5		S425-p	sIRCssVs_______

	mouse
T8	MSSILPFTPPIVKRL
K19	VKRLLGWKKGEQNGQ
K29	EQNGQEEKWCEKAVK
Y125	DEVCVNPYHYQRVEt
T132-p	YHYQRVEtPVLPPVL
T179	PQSNIPETPPPGYLS
S204	NHSMDAGSPNLSPNP
S208	DAGSPNLSPNPMSPA
S213	NLSPNPMSPAHNNLD
S309-p	EVFAECLsDSAIFVQ
K378	TIRMSFVKGWGAEYR
T388-p	GAEYRRQtVTSTPCW
S416	KVLTQMGSPSIRCSs
S418	LTQMGSPSIRCSsVs
S422	GSPSIRCSsVs____
S423-p	SPSIRCSsVs_____
S425-p	SIRCSsVs_______

	rat
T8	MSSILPFTPPIVKRL
K19	VKRLLGWKKGEQNGQ
K29	EQNGQEEKWCEKAVK
Y125	DEVCVNPYHYQRVET
T132	YHYQRVETPVLPPVL
T179	PQSNIPETPPPGYLS
S204	NHSMDAGSPNLSPNP
S208	DAGSPNLSPNPMSPA
S213	NLSPNPMSPAHNNLD
S309	EVFAECLSDSAIFVQ
K378	TIRMSFVKGWGAEYR
T388	GAEYRRQTVTSTPCW
S416	KVLTQMGSPSIRCSs
S418	LTQMGSPSIRCSsVs
S422	GSPSIRCSsVs____
S423-p	SPSIRCSsVs_____
S425-p	SIRCSsVs_______

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