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Protein Page:
p21Cip1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
p21Cip1 a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. Its expression is tightly controlled by p53, through which this protein mediates the p53-dependent cell cycle arrest at G1 phase. Note: This description may include information from UniProtKB.
Protein type: Inhibitor protein; Cell cycle regulation
Chromosomal Location of Human Ortholog: 6p21.2
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cyclin-dependent protein kinase holoenzyme complex; nucleolus; cytosol; nucleus
Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; cyclin binding; cyclin-dependent protein kinase activating kinase activity; metal ion binding; protein complex binding
Biological Process: nerve growth factor receptor signaling pathway; response to arsenic; response to toxin; protein amino acid phosphorylation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; negative regulation of cell proliferation; positive regulation of fibroblast proliferation; positive regulation of B cell proliferation; cell cycle arrest; G2/M transition of mitotic cell cycle; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to corticosterone stimulus; response to UV; epidermal growth factor receptor signaling pathway; response to drug; organ regeneration; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; negative regulation of cyclin-dependent protein kinase activity; response to organic nitrogen; positive regulation of programmed cell death; response to hyperoxia; positive regulation of protein kinase activity; Ras protein signal transduction; cellular response to extracellular stimulus; innate immune response; regulation of protein import into nucleus, translocation; negative regulation of phosphorylation; negative regulation of cell growth; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; response to DNA damage stimulus; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
Reference #:  P38936 (UniProtKB)
Alt. Names/Synonyms: CAP20; CDK-interacting protein 1; CDK-interaction protein 1; CDKN1; CDKN1A; CDN1A; CIP1; Cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; MDA-6; MDA6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein 6; p21; p21CIP1; p21Cip1/Waf1; PIC1; SDI1; WAF1; wild-type p53-activated fragment 1
Gene Symbols: CDKN1A
Molecular weight: 18,119 Da
Basal Isoelectric point: 8.69  Predict pI for various phosphorylation states
CST Pathways:  AMPK Signaling  |  G1/S Checkpoint  |  G2/M DNA Damage Checkpoint  |  PI3K/Akt Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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p21Cip1

Protein Structure Not Found.


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Sites Implicated In
cell cycle regulation: T57‑p, S130‑p, T145‑p
cell growth, altered: S146‑p
cell growth, inhibited: T145‑p
intracellular localization: T57‑p, S130‑p, T145‑p, S146‑p, S153‑p
molecular association, regulation: S130‑p, T145‑p, S146‑p, S153‑p
protein degradation: K16‑ub, T57‑p, K75‑ub, S114‑p, S130‑p, K141‑ub, K154‑ub, K161‑ub, K163‑ub
protein stabilization: T57‑p, S130‑p, T145‑p, S146‑p
ubiquitination: K16‑ub, T57‑p, K75‑ub, S130‑p, K141‑ub, K154‑ub, K161‑ub, K163‑ub

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S2 ______MSEPAGDVR
2 0 K16-ub RQNPCGSkACRRLFG
6 0 T57-p NFDFVTEtPLEGDFA
2 7 K75-ub VRGLGLPkLYLPtGP
1 0 Y77 GLGLPkLYLPtGPRR
2 3 P79 GLPkLYLPtGPRRGR
1 0 T80-p LPkLYLPtGPRRGRD
3 0 S98-p GGRRPGTsPALLQGT
1 0 S114-p EEDHVDLsLSCTLVP
1 0 S123-p SCTLVPRsGEQAEGs
8 4 S130-p sGEQAEGsPGGPGDS
1 0 K141-ac PGDSQGRkRRQtsMT
2 0 K141-ub PGDSQGRkRRQtsMT
16 0 T145-p QGRkRRQtsMTDFYH
9 0 S146-p GRkRRQtsMTDFYHs
2 0 S153-p sMTDFYHskRRLIFS
1 0 K154-ac MTDFYHskRRLIFSk
2 0 K154-ub MTDFYHskRRLIFSk
2 0 K161-ac kRRLIFSkRkP____
2 0 K161-ub kRRLIFSkRkP____
2 0 K163-ac RLIFSkRkP______
2 0 K163-ub RLIFSkRkP______
  mouse

 
S2-p ______MsNPGDVRP
K15 RPVPHRSKVCRCLFG
T56 NFDFVTETPLEGNFV
K74 VRSLGLPKVyLsPGS
Y76-p SLGLPKVyLsPGSRS
S78-p GLPKVyLsPGSRSRD
P79 LPKVyLsPGSRSRDD
S96 GDKRPSTSSALLQGP
S112 PEDHVALSLSCTLVS
- gap
S125 VSERPEDSPGGPGTS
K136 PGTSQGRKRRQtsLT
K136 PGTSQGRKRRQtsLT
T140-p QGRKRRQtsLTDFYH
S141-p GRKRRQtsLTDFYHS
S148 sLTDFYHSKRRLVFC
K149 LTDFYHSKRRLVFCK
K149 LTDFYHSKRRLVFCK
K156 KRRLVFCKRKP____
K156 KRRLVFCKRKP____
K158 RLVFCKRKP______
K158 RLVFCKRKP______
  dog

 
- gap
- gap
T25 NFDFVTETPLEGDFA
K43 VRGLGLSKVSLPAGP
S45 GLGLSKVSLPAGPRG
P47 GLSKVSLPAGPRGGR
A48 LSKVSLPAGPRGGRD
S66 GGKRPGTSPALLQGT
S82 QEDHLDLSLTCTLLP
S91-p TCTLLPHsPERPEAS
S98 sPERPEASPGVPGTS
K109 PGTSQGRKRRQTSMT
K109 PGTSQGRKRRQTSMT
T113 QGRKRRQTSMTDFYH
S114 GRKRRQTSMTDFYHS
S121 SMTDFYHSKRRLIFS
K122 MTDFYHSKRRLIFSK
K122 MTDFYHSKRRLIFSK
K129 KRRLIFSKRKP____
K129 KRRLIFSKRKP____
K131 RLIFSKRKP______
K131 RLIFSKRKP______
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