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NFkB-p65 (human)

Overview NFkB-p65 a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified. Note: This description may include information from UniProtKB. Protein type: DNA binding protein; Transcription factor; Nuclear receptor co-regulator Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; identical protein binding; transcription activator binding; protein N-terminus binding; protein kinase binding; transcription factor binding; NF-kappaB binding; protein binding; DNA binding; sequence-specific DNA binding; ubiquitin protein ligase binding; chromatin binding; phosphate binding; transcription factor activity Biological Process: response to cAMP; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; response to morphine; negative regulation of insulin receptor signaling pathway; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; response to insulin stimulus; activation of NF-kappaB transcription factor; response to organic substance; hair follicle development; positive regulation of cell proliferation; positive regulation of interferon type I production; inflammatory response; defense response to virus; toll-like receptor 4 signaling pathway; aging; response to drug; positive regulation of Schwann cell differentiation; membrane protein intracellular domain proteolysis; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; acetaldehyde metabolic process; liver development; response to amino acid stimulus; Toll signaling pathway; toll-like receptor 1 signaling pathway; response to UV-B; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; organ morphogenesis; positive regulation of chondrocyte differentiation; positive regulation of interleukin-12 biosynthetic process; response to hydrogen peroxide; response to mechanical stimulus; response to muramyl dipeptide; virus-host interaction; regulation of inflammatory response; toll-like receptor signaling pathway; cellular defense response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to progesterone stimulus; negative regulation of transcription, DNA-dependent; negative regulation of protein catabolic process; response to cobalamin; negative regulation of apoptosis Reference #:  Q04206 (UniProtKB) Alt. Names/Synonyms: MGC131774; NFKB3; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65; RELA; TF65; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)); v-rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65 Gene Symbols: RELA Molecular weight: 60,219 Da Basal Isoelectric point: 5.46  Predict pI for various phosphorylation states CST Pathways:  Apoptosis  |  B Cell Receptor Signaling  |  Death Receptor Signaling  |  Inhibition of Apoptosis  |  NF-kB Signaling  |  Signaling Pathways Activating p38 MAPK  |  T Cell Receptor Signaling  |  Toll-like Receptors Pathway Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below NFkB-p65 1NFI - A/C=20-320 (human) 2LSP - A=304-316 (human) 2O61 - A=20-291 (human) 3GUT - A/C/G/E=20-291 (human) 3QXY - Q/P=302-316 (human) 3RC0 - Q/P=302-316 (human) 1BFT - A/B=191-291 (mouse) 1IKN - A=19-304 (mouse) 1K3Z - A/B=191-326 (mouse) 1LE5 - A/E=20-291 (mouse) 1LE9 - A/E=20-291 (mouse) 1LEI - A=20-291 (mouse) 1MY5 - A/B=191-304 (mouse) 1MY7 - A/B=191-304 (mouse) 1OY3 - B/C=191-326 (mouse) 1RAM - A/B=19-291 (mouse) 1VKX - A=20-291 (mouse) 2I9T - A=19-291 (mouse) 2RAM - A/B=19-291 (mouse)

STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 1NFI 2LSP 2O61 3GUT 3QXY 3RC0  |  Phospho3D  |  DISEASE  |  Source  |  NURSA  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene

	Sites Implicated In
apoptosis, altered: S536‑p apoptosis, inhibited: S276‑p, T505‑p cell growth, altered: S536‑p cell growth, inhibited: T505‑p cell motility, altered: S536‑p cell motility, inhibited: T505‑p cytoskeletal reorganization: T505‑p transcription, altered: K195‑u, S205‑p, T254‑p, S276‑p, S281‑p, S311‑p, T435‑p, S468‑p, T505‑p, S529‑p, S536‑p transcription, induced: S276‑p transcription, inhibited: S468‑p activation: T254‑p, S276‑p, S468‑p, S529‑p, S536‑p inhibition: K195‑u, S468‑p intracellular localization: S205‑p, T254‑p, S276‑p, S281‑p, S468‑p, S529‑p, S536‑p molecular association, regulation: T254‑p, S276‑p, S536‑p protein degradation: K195‑u, S468‑p protein stabilization: T254‑p, S276‑p, S536‑p ubiquitination: S468‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
1	0		K37-m1	RGMRFRYkCEGRsAG
0	1		S42-p	RYkCEGRsAGsIPGE
0	1		S45-p	CEGRsAGsIPGERST
1	0		K56-u	ERSTDTTkTHPTIkI
1	0		K62-u	TkTHPTIkINGYTGP
0	1		T71-p	NGYTGPGtVRISLVT
1	0		K79-u	VRISLVTkDPPHRPH
0	2		F99	GKDCRDGFYEAELCP
2	24		K122-a	NLGIQCVkkRDLEQA
1	0		K122-u	NLGIQCVkkRDLEQA
2	0		K123-a	LGIQCVkkRDLEQAI
1	1		K123-u	LGIQCVkkRDLEQAI
0	1		S131-p	RDLEQAIsQRIQTNN
0	2		P176	SGRPLRLPPVLSHPI
2	0		K195-u	APNTAELkICRVNRN
3	0		S205-p	RVNRNSGsCLGGDEI
3	0		K218-a	EIFLLCDkVQkEDIE
6	0		K221-a	LLCDkVQkEDIEVYF
2	0		T254-p	QVAIVFRtPPYADPS
62	0		S276-p	SMQLRRPsDRELsEP
4	0		S281-p	RPsDRELsEPMEFQY
0	1		T305-p	IEEKRKRtyEtFksI
1	0		T305-g	IEEKRKRtyEtFksI
0	1		Y306-p	EEKRKRtyEtFksIM
0	1		T308-p	KRKRtyEtFksIMkk
24	1		K310-a	KRtyEtFksIMkkSP
1	0		K310-m1	KRtyEtFksIMkkSP
1	0		K310-u	KRtyEtFksIMkkSP
9	1		S311-p	RtyEtFksIMkkSPF
3	0		K314-a	EtFksIMkkSPFSGP
2	0		K314-m1	EtFksIMkkSPFSGP
1	0		K314-u	EtFksIMkkSPFSGP
3	0		K315-a	tFksIMkkSPFSGPt
2	0		K315-m1	tFksIMkkSPFSGPt
1	0		K315-u	tFksIMkkSPFSGPt
1	0		T322-g	kSPFSGPtDPRPPPR
1	0		T352-g	APQPYPFtSSLSTIN
2	0		T435-p	PTQAGEGtLSEALLQ
12	0		S468-p	AVFTDLAsVDNSEFQ
3	0		T505-p	EAITRLVtGAQRPPD
12	2		S529-p	GLPNGLLsGDEDFSs
101	0		S536-p	sGDEDFSsIADMDFS
0	1		S547-p	MDFSALLsQISS___

	mouse
K37	RGMRFRYKCEGRSAG
S42	RYKCEGRSAGSIPGE
S45	CEGRSAGSIPGERST
K56	ERSTDTTKTHPTIKI
K62	TKTHPTIKINGYTGP
T71	NGYTGPGTVRISLVT
K79	VRISLVTKDPPHRPH
Y99-p	GKDCRDGyYEADLCP
K122-a	NLGIQCVkkRDLEQA
K122	NLGIQCVKkRDLEQA
K123	LGIQCVkKRDLEQAI
K123-u	LGIQCVkkRDLEQAI
S131	RDLEQAISQRIQTNN
T176-p	AGRPLLLtPVLSHPI
K195	APNTAELKICRVNRN
S205	RVNRNSGSCLGGDEI
K218	EIFLLCDKVQkEDIE
K221-a	LLCDKVQkEDIEVYF
T254	QVAIVFRTPPYADPS
S276-p	SMQLRRPsDRELSEP
S281	RPsDRELSEPMEFQY
T305	IEEKRKRTYETFksI
T305	IEEKRKRTYETFksI
Y306	EEKRKRTYETFksIM
T308	KRKRTYETFksIMKK
K310-a	KRTYETFksIMKKSP
K310	KRTYETFKsIMKKSP
K310	KRTYETFKsIMKKSP
S311-p	RTYETFksIMKKSPF
K314	ETFksIMKKSPFNGP
K314	ETFksIMKKSPFNGP
K314	ETFksIMKKSPFNGP
K315	TFksIMKKSPFNGPT
K315	TFksIMKKSPFNGPT
K315	TFksIMKKSPFNGPT
T322	KSPFNGPTEPRPPTR
P352	APQPYTFPASLSTIN
T433	STQAGEGTLSEALLH
S467-p	GVFTDLAsVDNSEFQ
T504-p	EAITRLVtGSQRPPD
S527	SGLPNGLSGDEDFSs
S534-p	SGDEDFSsIADMDFS
S545	MDFSALLSQISS___

	rat
K37	RGMRFRYKCEGRSAG
S42	RYKCEGRSAGSIPGE
S45	CEGRSAGSIPGERST
K56	ERSTDTTKTHPTIKI
K62	TKTHPTIKINGYTGP
T71	NGYTGPGTVRISLVT
K79	VRISLVTKDPPHRPH
F99	GKDCRDGFYEAELCP
K122	NLGIQCVKKRDLEQA
K122	NLGIQCVKKRDLEQA
K123	LGIQCVKKRDLEQAI
K123	LGIQCVKKRDLEQAI
S131	RDLEQAISQRIQTNN
T176	SGRPLRLTPVLSHPI
K195	APNTAELKICRVNRN
S205	RVNRNSGSCLGGDEI
K218	EIFLLCDKVQKEDIE
K221	LLCDKVQKEDIEVYF
T254-p	QVAIVFRtPPYADPS
S276-p	SMQLRRPsDRELSEP
S281	RPsDRELSEPMEFQY
T305	IEEKRKRTYETFksI
T305	IEEKRKRTYETFksI
Y306	EEKRKRTYETFksIM
T308	KRKRTYETFksIMKK
K310-a	KRTYETFksIMKKSP
K310	KRTYETFKsIMKKSP
K310	KRTYETFKsIMKKSP
S311-p	RTYETFksIMKKSPF
K314	ETFksIMKKSPFNGP
K314	ETFksIMKKSPFNGP
K314	ETFksIMKKSPFNGP
K315	TFksIMKKSPFNGPT
K315	TFksIMKKSPFNGPT
K315	TFksIMKKSPFNGPT
T322	KSPFNGPTEPRPPPR
S352	APQPYAFSTSLSTIN
T434	STQAGEGTLSEALLH
S468-p	GVFTDLAsVDNSEFQ
T505	EAITRLVTGSQRPPD
S528-p	SGLPNGLsGDEDFSs
S535-p	sGDEDFSsIADMDFS
S546	MDFSALLSQISS___

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