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Protein Page:
NFkB-p65 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NFkB-p65 a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Transcription factor; Nuclear receptor co-regulator
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; NF-kappaB binding; identical protein binding; protein binding; DNA binding; transcription activator binding; ubiquitin protein ligase binding; protein complex binding; protein N-terminus binding; chromatin binding; phosphate binding; protein kinase binding; transcription factor activity; transcription factor binding
Biological Process: nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of transcription, DNA-dependent; negative regulation of insulin receptor signaling pathway; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; hair follicle development; toll-like receptor 4 signaling pathway; defense response to virus; response to drug; membrane protein intracellular domain proteolysis; positive regulation of I-kappaB kinase/NF-kappaB cascade; acetaldehyde metabolic process; response to amino acid stimulus; toll-like receptor 2 signaling pathway; organ morphogenesis; positive regulation of chondrocyte differentiation; response to muramyl dipeptide; response to mechanical stimulus; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; response to progesterone stimulus; negative regulation of apoptosis; transcription from RNA polymerase II promoter; response to cAMP; response to morphine; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 10 signaling pathway; response to insulin stimulus; response to organic substance; positive regulation of cell proliferation; positive regulation of interferon type I production; inflammatory response; aging; positive regulation of Schwann cell differentiation; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; liver development; response to UV-B; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-12 biosynthetic process; regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; cellular defense response; response to cobalamin
Reference #:  Q04206 (UniProtKB)
Alt. Names/Synonyms: MGC131774; NFKB3; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65; RELA; TF65; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)); v-rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65
Gene Symbols: RELA
Molecular weight: 60,219 Da
Basal Isoelectric point: 5.46  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  B Cell Receptor Signaling  |  Death Receptor Signaling  |  Inhibition of Apoptosis  |  NF-kB Signaling  |  Regulation of P38 MAPKs  |  T Cell Receptor Signaling  |  Toll-Like Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NFkB-p65

Protein Structure Not Found.


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Sites Implicated In
apoptosis, altered: S536‑p
apoptosis, inhibited: S276‑p, T505‑p
cell growth, altered: S536‑p
cell growth, inhibited: T505‑p
cell motility, altered: S536‑p
cell motility, inhibited: T505‑p
cytoskeletal reorganization: T505‑p
transcription, altered: K195‑ub, S205‑p, T254‑p, S276‑p, S281‑p, S468‑p, T505‑p, S529‑p, S536‑p
transcription, induced: S205‑p, S276‑p, S281‑p, S468‑p, S529‑p, S536‑p
transcription, inhibited: S276‑p, T435‑p, S468‑p
activity, induced: T254‑p, S276‑p, S468‑p, S529‑p, S536‑p
activity, inhibited: K195‑ub, S468‑p
intracellular localization: S205‑p, T254‑p, S276‑p, S281‑p, S468‑p, S529‑p, S536‑p
molecular association, regulation: T254‑p, S276‑p, S536‑p
protein degradation: K195‑ub, S468‑p
protein stabilization: T254‑p, S276‑p, S536‑p
ubiquitination: S468‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 0 K37-me RGMRFRYkCEGRsAG
1 0 K37-m1 RGMRFRYkCEGRsAG
0 1 S42-p RYkCEGRsAGsIPGE
0 3 S45-p CEGRsAGsIPGERST
1 0 K56-ub ERSTDTTkTHPTIkI
1 0 K62-ub TkTHPTIkINGYTGP
0 1 T71-p NGYTGPGtVRISLVT
1 0 K79-ub VRISLVTkDPPHRPH
0 2 F99 GKDCRDGFYEAELCP
2 25 K122-ac NLGIQCVkkRDLEQA
1 0 K122-ub NLGIQCVkkRDLEQA
2 0 K123-ac LGIQCVkkRDLEQAI
1 1 K123-ub LGIQCVkkRDLEQAI
0 1 S131-p RDLEQAIsQRIQTNN
0 2 P176 SGRPLRLPPVLSHPI
2 0 K195-ub APNTAELkICRVNRN
4 0 S205-p RVNRNSGsCLGGDEI
3 0 K218-ac EIFLLCDkVQkEDIE
1 0 K218-me EIFLLCDkVQkEDIE
6 0 K221-ac LLCDkVQkEDIEVYF
1 0 K221-me LLCDkVQkEDIEVYF
2 1 T254-p QVAIVFRtPPYADPS
0 1 Y257 IVFRtPPYADPSLQA
64 0 S276-p SMQLRRPsDRELsEP
5 0 S281-p RPsDRELsEPMEFQY
0 1 T305-p IEEKRKRtyEtFksI
1 0 T305-gl IEEKRKRtyEtFksI
0 1 Y306-p EEKRKRtyEtFksIM
0 1 T308-p KRKRtyEtFksIMkk
36 1 K310-ac KRtyEtFksIMkksP
1 0 K310-m1 KRtyEtFksIMkksP
1 0 K310-ub KRtyEtFksIMkksP
9 1 S311-p RtyEtFksIMkksPF
3 0 K314-ac EtFksIMkksPFSGP
2 0 K314-m1 EtFksIMkksPFSGP
1 0 K314-ub EtFksIMkksPFSGP
3 0 K315-ac tFksIMkksPFSGPt
2 0 K315-m1 tFksIMkksPFSGPt
1 0 K315-ub tFksIMkksPFSGPt
0 1 S316-p FksIMkksPFSGPtD
1 0 T322-gl ksPFSGPtDPRPPPR
1 0 T352-gl APQPYPFtSSLSTIN
2 0 T435-p PTQAGEGtLSEALLQ
13 0 S468-p AVFTDLAsVDNSEFQ
3 0 T505-p EAITRLVtGAQRPPD
12 2 S529-p GLPNGLLsGDEDFSs
107 0 S536-p sGDEDFSsIADMDFS
0 1 S547-p MDFSALLsQISS___
3045 : Acetyl-NF-kappaB p65 (Lys310) Antibody
12629 : Acetyl-NF-kappa-B p65 (Lys310) (D2S3J) Rabbit mAb
3039 : Phospho-NF-KappaB p65 (Ser468) Antibody
3031 : Phospho-NF-KappaB p65 (Ser536) Antibody
3033 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb
3036 : Phospho-NF-KappaB p65 (Ser536) (7F1) Mouse mAb
4025 : Phospho-NF-kB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated)
4886 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
4887 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
5733 : Phospho-NF-¿¿B p65 (Ser536) (93H1) Rabbit mAb (PE Conjugate)
13346 : Phospho-NF-¿B p65 (Ser536) (E1Z1T) Mouse mAb
  mouse

 
K37 RGMRFRYKCEGRSAG
K37 RGMRFRYKCEGRSAG
S42 RYKCEGRSAGSIPGE
S45 CEGRSAGSIPGERST
K56 ERSTDTTKTHPTIKI
K62 TKTHPTIKINGYTGP
T71 NGYTGPGTVRISLVT
K79 VRISLVTKDPPHRPH
Y99-p GKDCRDGyYEADLCP
K122-ac NLGIQCVkkRDLEQA
K122 NLGIQCVKkRDLEQA
K123 LGIQCVkKRDLEQAI
K123-ub LGIQCVkkRDLEQAI
S131 RDLEQAISQRIQTNN
T176-p AGRPLLLtPVLSHPI
K195 APNTAELKICRVNRN
S205 RVNRNSGSCLGGDEI
K218 EIFLLCDKVQkEDIE
K218 EIFLLCDKVQkEDIE
K221-ac LLCDKVQkEDIEVYF
K221 LLCDKVQKEDIEVYF
T254 QVAIVFRTPPYADPS
Y257 IVFRTPPYADPSLQA
S276-p SMQLRRPsDRELSEP
S281 RPsDRELSEPMEFQY
T305 IEEKRKRTYETFksI
T305 IEEKRKRTYETFksI
Y306 EEKRKRTYETFksIM
T308 KRKRTYETFksIMKK
K310-ac KRTYETFksIMKKSP
K310 KRTYETFKsIMKKSP
K310 KRTYETFKsIMKKSP
S311-p RTYETFksIMKKSPF
K314 ETFksIMKKSPFNGP
K314 ETFksIMKKSPFNGP
K314 ETFksIMKKSPFNGP
K315 TFksIMKKSPFNGPT
K315 TFksIMKKSPFNGPT
K315 TFksIMKKSPFNGPT
S316 FksIMKKSPFNGPTE
T322 KSPFNGPTEPRPPTR
P352 APQPYTFPASLSTIN
T433 STQAGEGTLSEALLH
S467-p GVFTDLAsVDNSEFQ
T504-p EAITRLVtGSQRPPD
S527 SGLPNGLSGDEDFSs
S534-p SGDEDFSsIADMDFS
S545 MDFSALLSQISS___
3045 : Acetyl-NF-kappaB p65 (Lys310) Antibody
12629 : Acetyl-NF-kappa-B p65 (Lys310) (D2S3J) Rabbit mAb
3039 : Phospho-NF-KappaB p65 (Ser468) Antibody
3031 : Phospho-NF-KappaB p65 (Ser536) Antibody
3033 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb
3036 : Phospho-NF-KappaB p65 (Ser536) (7F1) Mouse mAb
4025 : Phospho-NF-kB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated)
4886 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
4887 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
5733 : Phospho-NF-¿¿B p65 (Ser536) (93H1) Rabbit mAb (PE Conjugate)
13346 : Phospho-NF-¿B p65 (Ser536) (E1Z1T) Mouse mAb
  rat

 
K37 RGMRFRYKCEGRSAG
K37 RGMRFRYKCEGRSAG
S42 RYKCEGRSAGSIPGE
S45 CEGRSAGSIPGERST
K56 ERSTDTTKTHPTIKI
K62 TKTHPTIKINGYTGP
T71 NGYTGPGTVRISLVT
K79 VRISLVTKDPPHRPH
F99 GKDCRDGFYEAELCP
K122 NLGIQCVKKRDLEQA
K122 NLGIQCVKKRDLEQA
K123 LGIQCVKKRDLEQAI
K123 LGIQCVKKRDLEQAI
S131 RDLEQAISQRIQTNN
T176 SGRPLRLTPVLSHPI
K195 APNTAELKICRVNRN
S205 RVNRNSGSCLGGDEI
K218 EIFLLCDKVQKEDIE
K218 EIFLLCDKVQKEDIE
K221 LLCDKVQKEDIEVYF
K221 LLCDKVQKEDIEVYF
T254-p QVAIVFRtPPyADPS
Y257-p IVFRtPPyADPSLQA
S276-p SMQLRRPsDRELSEP
S281 RPsDRELSEPMEFQY
T305 IEEKRKRTYETFksI
T305 IEEKRKRTYETFksI
Y306 EEKRKRTYETFksIM
T308 KRKRTYETFksIMKK
K310-ac KRTYETFksIMKKSP
K310 KRTYETFKsIMKKSP
K310 KRTYETFKsIMKKSP
S311-p RTYETFksIMKKSPF
K314 ETFksIMKKSPFNGP
K314 ETFksIMKKSPFNGP
K314 ETFksIMKKSPFNGP
K315 TFksIMKKSPFNGPT
K315 TFksIMKKSPFNGPT
K315 TFksIMKKSPFNGPT
S316 FksIMKKSPFNGPTE
T322 KSPFNGPTEPRPPPR
S352 APQPYAFSTSLSTIN
T434 STQAGEGTLSEALLH
S468-p GVFTDLAsVDNSEFQ
T505 EAITRLVTGSQRPPD
S528-p SGLPNGLsGDEDFSs
S535-p sGDEDFSsIADMDFS
S546 MDFSALLSQISS___
3045 : Acetyl-NF-kappaB p65 (Lys310) Antibody
3039 : Phospho-NF-KappaB p65 (Ser468) Antibody
3031 : Phospho-NF-KappaB p65 (Ser536) Antibody
3033 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb
3036 : Phospho-NF-KappaB p65 (Ser536) (7F1) Mouse mAb
4025 : Phospho-NF-kB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated)
4886 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
4887 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
5733 : Phospho-NF-¿¿B p65 (Ser536) (93H1) Rabbit mAb (PE Conjugate)
13346 : Phospho-NF-¿B p65 (Ser536) (E1Z1T) Mouse mAb
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