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Protein Page:
NFAT1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NFAT1 a transcription factor that plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha and GM-CSF. It is present in a phosphorylated the cytosol of unstimulated T cells. After T-cell activation, it is dephosphorylated by calcineurin and translocates to the nucleus as a part of a transcription complex. Two splice variant isoforms have been identified. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Transcription factor
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; plasma membrane; nucleolus; nucleus; cytosol; ribonucleoprotein complex; actin cytoskeleton
Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity
Biological Process: response to drug; cell migration; B cell receptor signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of B cell proliferation; innate immune response; cytokine production; positive regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus
Reference #:  Q13469 (UniProtKB)
Alt. Names/Synonyms: NF-ATc2; NF-ATp; NFAC2; NFAT pre-existing subunit; NFAT transcription complex, preexisting component; NFAT1; NFATC2; NFATP; Nuclear factor of activated T-cells, cytoplasmic 2; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2; nuclear factor of activated T-cells, preexisting component; preexisting nuclear factor of activated T-cells 2; T cell transcription factor NFAT1; T-cell transcription factor NFAT1
Gene Symbols: NFATC2
Molecular weight: 100,146 Da
Basal Isoelectric point: 6.87  Predict pI for various phosphorylation states
CST Pathways:  B Cell Receptor Signaling  |  T Cell Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NFAT1

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 - gap
0 1 S23-p PGHEPGGsPQDELDF
0 20 Y36-p DFSILFDyEyLNPNE
0 8 Y38-p SILFDyEyLNPNEEE
0 5 S53-p PNAHKVAsPPSGPAY
0 1 P54 NAHKVAsPPSGPAYP
0 2 S56 HKVAsPPSGPAYPDD
0 1 S73-p DYGLKPYsPLASLSG
0 3 S99-p VGPQKFLsAAKPAGA
0 3 S107-p AAKPAGAsGLsPRIE
1 2 S110-p PAGAsGLsPRIEItP
1 2 T116-p LsPRIEItPSHELIQ
0 7 L136 RMRDAGLLVEQPPLA
0 8 S148-p PLAGVAAsPRFTLPV
2 1 S168 YREPLCLSPASSGSS
0 1 S171 PLCLSPASSGSSASF
0 1 S172 LCLSPASSGSSASFI
0 1 S174 LSPASSGSSASFISD
0 1 S175 SPASSGSSASFISDT
0 1 S177 ASSGSSASFISDTFS
0 1 S180 GSSASFISDTFSPYT
0 1 Y212-p FQNIPAHysPRTsPI
1 5 S213-p QNIPAHysPRTsPIM
0 4 T216 PAHysPRTsPIMsPR
2 8 S217-p AHysPRTsPIMsPRT
2 13 S221-p PRTsPIMsPRTsLAE
0 1 S225-p PIMsPRTsLAEDsCL
0 1 S230-p RTsLAEDsCLGRHsP
0 10 S236-p DsCLGRHsPVPRPAs
0 10 S243-p sPVPRPAsRsSsPGA
0 2 S245-p VPRPAsRsSsPGAKR
0 4 S247-p RPAsRsSsPGAKRRH
0 2 S255-p PGAKRRHsCAEALVA
0 5 S268-p VALPPGAsPQRSRSP
0 1 S274 AsPQRSRSPSPQPSS
0 1 S276 PQRSRSPSPQPSSHV
0 1 S280 RSPSPQPSSHVAPQD
0 5 T325-p IPPKMWKtsPDPsPV
0 10 S326-p PPKMWKtsPDPsPVs
1 5 S330-p WKtsPDPsPVsAAPs
0 3 S333-p sPDPsPVsAAPsKAG
0 3 S337-p sPVsAAPsKAGLPRH
0 7 Y346-p AGLPRHIyPAVEFLG
0 12 S363-p EQGERRNsAPESILL
0 1 T374-p SILLVPPtWPKPLVP
0 1 T483-p VHRITGKtVtTTSYE
0 1 T485-p RITGKtVtTTSYEKI
0 1 T585-p PMVERQDtDSCLVYG
0 1 T599-p GGQQMILtGQNFTsE
0 1 S605-p LtGQNFTsESKVVFT
0 1 T615 KVVFTEKTTDGQQIW
0 1 T616 VVFTEKTTDGQQIWE
0 1 T652-p YRNKHIRtPVKVNFY
1 0 K682 YHPVPAIKTEPTDEY
0 2 S736-p QQFRTGLssPDARYQ
0 1 S737-p QFRTGLssPDARYQQ
0 18 Y752-p QNPAAVLyQRsKsLs
0 3 S755-p AAVLyQRsKsLsPsL
0 6 S757-p VLyQRsKsLsPsLLG
0 9 S759-p yQRsKsLsPsLLGYQ
0 1 S761-p RsKsLsPsLLGYQQP
0 1 S776-p ALMAAPLsLADAHRS
0 4 S790-p SVLVHAGsQGQSSAL
0 1 S808-p SPTNQQAsPVIHYSP
0 10 S856-p VSQGQRLsPGsyPTV
0 2 S859-p GQRLsPGsyPTVIQQ
0 3 Y860-p QRLsPGsyPTVIQQQ
0 1 S882-p AKNGPPVsDQKEVLP
0 1 T893-p EVLPAGVtIKQEQNL
1 0 K895 LPAGVtIKQEQNLDQ
0 1 Y904-p EQNLDQTyLDDVNEI
  mouse

► Hide Isoforms
 
- gap
S23 PGHEPGGSPQDELDF
Y36 DFSILFDYDYLNPIE
Y38 SILFDYDYLNPIEEE
S53-p PIAHKAIssPsGLAY
S54-p IAHKAIssPsGLAYP
S56-p HKAIssPsGLAYPDD
N73 DYGLKPCNPLASLSG
S99-p IGFQNFLsPVKPAGA
S107-p PVKPAGAsGPsPRIE
S110-p PAGAsGPsPRIEItP
T116-p PsPRIEItPSHELMQ
S136-p RGRDAGLsPEQPALA
S150-p ALAGVAAsPRFTLPV
S170-p YREPLCLsPAssGss
S173-p PLCLsPAssGssAsF
S174-p LCLsPAssGssAsFI
S176-p LsPAssGssAsFIsD
S177-p sPAssGssAsFIsDT
S179-p AssGssAsFIsDTFS
S182-p GssAsFIsDTFSPYT
Y214 FQNIPAHYsPRtsPI
S215-p QNIPAHYsPRtsPIM
T218-p PAHYsPRtsPIMsPR
S219-p AHYsPRtsPIMsPRT
S223-p PRtsPIMsPRTSLAE
S227 PIMsPRTSLAEDSCL
S232 RTSLAEDSCLGRHsP
S238-p DSCLGRHsPVPRPAs
S245-p sPVPRPAsRSSSPGA
S247 VPRPAsRSSSPGAKR
S249 RPAsRSSSPGAKRRH
S257-p PGAKRRHsCAEALVA
S270-p VAPLPAAsPQRSRsP
S276-p AsPQRSRsPsPQPsP
S278-p PQRSRsPsPQPsPHV
S282-p RsPsPQPsPHVALQD
T327-p IPSKIWKtsPDPtPV
S328-p PSKIWKtsPDPtPVS
T332-p WKtsPDPtPVSTAPS
S335 sPDPtPVSTAPSKAG
S339 tPVSTAPSKAGLARH
Y348-p AGLARHIyPTVEFLG
S365-p EQEERRNsAPESILL
T376 SILLVPPTWPKQLVP
T485 VHRITGKTVTTTSYE
T487 RITGKTVTTTSYEKI
M587 PMVERQDMDSCLVYG
T601 GGQQMILTGQNFTAE
A607 LTGQNFTAESKVVFM
T617-p KVVFMEKttDGQQIW
T618-p VVFMEKttDGQQIWE
V654 YRNKHIRVPVKVNFY
K684-sm YHPVPAIkTEPSDEY
S738 QQFRSGLSSPDARYQ
S739 QFRSGLSSPDARYQQ
Y754 QSPAAALYQRsKsLs
S757-p AAALYQRsKsLsPGL
S759-p ALYQRsKsLsPGLLG
S761-p YQRsKsLsPGLLGYQ
G763 RsKsLsPGLLGYQQP
G778 SLLAAPLGLADAHRS
S792 SVLVHAGSQGQGQGS
S812 SSASQQASPVIHYSP
S860-p INQGQRLsPGAYPTV
A863 GQRLsPGAYPTVIQQ
Y864 QRLsPGAYPTVIQQQ
S884 RAAKNGPSDQKEALP
T895 EALPTGVTVkQEQNL
K897-sm LPTGVTVkQEQNLDQ
Y906 EQNLDQTYLDDVNEI
  NFAT1 iso2  
S20-p QPLRTMGsADREEPI
- gap
- gap
- gap
S33 PIAHKAISSPSGLAY
S34 IAHKAISSPSGLAYP
S36 HKAISSPSGLAYPDD
N53 DYGLKPCNPLASLSG
S79 IGFQNFLSPVKPAGA
S87 PVKPAGASGPSPRIE
S90 PAGASGPSPRIEITP
T96 PSPRIEITPSHELMQ
S116 RGRDAGLSPEQPALA
S130 ALAGVAASPRFTLPV
S150 YREPLCLSPASSGSS
S153 PLCLSPASSGSSASF
S154 LCLSPASSGSSASFI
S156 LSPASSGSSASFISD
S157 SPASSGSSASFISDT
S159 ASSGSSASFISDTFS
S162 GSSASFISDTFSPYT
Y194 FQNIPAHYSPRTSPI
S195 QNIPAHYSPRTSPIM
T198 PAHYSPRTSPIMSPR
S199 AHYSPRTSPIMSPRT
S203 PRTSPIMSPRTSLAE
S207 PIMSPRTSLAEDSCL
S212 RTSLAEDSCLGRHSP
S218 DSCLGRHSPVPRPAS
S225 SPVPRPASRSSSPGA
S227 VPRPASRSSSPGAKR
S229 RPASRSSSPGAKRRH
S237 PGAKRRHSCAEALVA
S250 VAPLPAASPQRSRSP
S256 ASPQRSRSPSPQPSP
S258 PQRSRSPSPQPSPHV
S262 RSPSPQPSPHVALQD
T307 IPSKIWKTSPDPTPV
S308 PSKIWKTSPDPTPVS
T312 WKTSPDPTPVSTAPS
S315 SPDPTPVSTAPSKAG
S319 TPVSTAPSKAGLARH
Y328-p AGLARHIyPTVEFLG
S345 EQEERRNSAPESILL
T356 SILLVPPTWPKQLVP
T465 VHRITGKTVTTTSYE
T467 RITGKTVTTTSYEKI
M567 PMVERQDMDSCLVYG
T581 GGQQMILTGQNFTAE
A587 LTGQNFTAESKVVFM
T597 KVVFMEKTTDGQQIW
T598 VVFMEKTTDGQQIWE
V634 YRNKHIRVPVKVNFY
K664 YHPVPAIKTEPSDEY
S718 QQFRSGLSSPDARYQ
S719 QFRSGLSSPDARYQQ
Y734 QSPAAALYQRSKSLS
S737 AAALYQRSKSLSPGL
S739 ALYQRSKSLSPGLLG
S741 YQRSKSLSPGLLGYQ
G743 RSKSLSPGLLGYQQP
G758 SLLAAPLGLADAHRS
S772 SVLVHAGSQGQGQGS
S792 SSASQQASPVIHYSP
S840 INQGQRLSPGAYPTV
A843 GQRLSPGAYPTVIQQ
Y844 QRLSPGAYPTVIQQQ
S864 RAAKNGPSDQKEALP
T875 EALPTGVTVKQEQNL
K877 LPTGVTVKQEQNLDQ
Y886 EQNLDQTYLDDELID
  rat

 
- gap
S23 PGHEPGGSSQDELDF
Y36 DFSILFDYDYLNPIE
Y38 SILFDYDYLNPIEEE
S53 PIAHKAISSPSGLAY
S54 IAHKAISSPSGLAYP
S56 HKAISSPSGLAYPDD
N73 DYGLKPCNPLASLSG
S99 IGFQNFLSPAKPAGA
S107 PAKPAGASGPSPRIE
S110 PAGASGPSPRIEITP
T116 PSPRIEITPSHELMQ
S136-p RGRDAGLsPEQPALA
S150 ALAGMAASPRFTLPV
S170 YREPLCLSPASSGSS
S173 PLCLSPASSGSSASF
S174 LCLSPASSGSSASFI
S176 LSPASSGSSASFISD
S177 SPASSGSSASFISDT
S179 ASSGSSASFISDTFS
S182 GSSASFISDTFSPYT
Y214 FQNIPAHYSPRTSPI
S215 QNIPAHYSPRTSPIM
T218 PAHYSPRTSPIMSPR
S219 AHYSPRTSPIMSPRT
S223 PRTSPIMSPRTSLAE
S227 PIMSPRTSLAEDSCL
S232 RTSLAEDSCLGRHSP
S238 DSCLGRHSPVPRPAS
S245 SPVPRPASRSSSPGA
S247 VPRPASRSSSPGAKR
S249 RPASRSSSPGAKRRH
S257 PGAKRRHSCAEALVA
S270-p VAPLPAAsPQRSRSP
S276 AsPQRSRSPSPQPSP
S278 PQRSRSPSPQPSPHV
S282 RSPSPQPSPHVAPQD
T327 IPSKIWKTSPDPTPV
S328 PSKIWKTSPDPTPVS
T332 WKTSPDPTPVSTAPS
S335 SPDPTPVSTAPSKAG
S339 TPVSTAPSKAGLARH
Y348 AGLARHIYPAVEFLG
S365-p EQEERRNsAPESILL
T376 SILLVPPTWPKQLVP
T485 VHRITGKTVTTTSYE
T487 RITGKTVTTTSYEKI
M587 PMVERQDMDSCLVYG
T601 GGQQMILTGQNFTAE
A607 LTGQNFTAESKVVFM
T617 KVVFMEKTTDGQQIW
T618 VVFMEKTTDGQQIWE
V654 YRNKHIRVPVKVNFY
K684 YHPVPAIKTEPSDEY
S738 QQFRSGLSSPDARYQ
S739 QFRSGLSSPDARYQQ
Y754 QSPAAALYQRSKSLS
S757 AAALYQRSKSLSPGL
S759 ALYQRSKSLSPGLLG
S761 YQRSKSLSPGLLGYQ
G763 RSKSLSPGLLGYQQP
G778 SLLAAPLGLADAHRS
S792 SVLVHAGSQGQGQGQ
S814 SSASQQASPVIHYSP
S862 VNQGQRLSPGSYPTV
S865 GQRLSPGSYPTVIQQ
Y866 QRLSPGSYPTVIQQQ
S888 AKNGPPVSDQKEALP
T899 EALPSGVTVKQEQNL
K901 LPSGVTVKQEQNLDQ
Y910 EQNLDQTYLDDVNEI
  chicken

 
- gap
S30 PGPARDGSPQEEFDF
Y43 DFSILFDYDYLKPIE
Y45 SILFDYDYLKPIEEE
S60 PTPHKTNSPPSGVAY
P61 TPHKTNSPPSGVAYA
S63 HKTNSPPSGVAYAHD
P80 DCGLKPCPLPFPSLS
H106 SIGTAPTHPASAAGA
S114 PASAAGASAPSPRIE
S117 AAGASAPSPRIEITS
T123 PSPRIEITSYPELIN
L143 RSRDTDVLLVEHQSN
S155 QSNSATTSPRVTLPV
S175-p YREPLCLsPASSGSS
S178 PLCLsPASSGSSASF
S179 LCLsPASSGSSASFI
S181 LsPASSGSSASFISE
S182 sPASSGSSASFISET
S184 ASSGSSASFISETNL
S187 GSSASFISETNLSPC
Y220 FQNIHTHYSPRTSPI
S221 QNIHTHYSPRTSPIM
T224 HTHYSPRTSPIMSPR
S225 THYSPRTSPIMSPRT
S229 PRTSPIMSPRTSITE
S233 PIMSPRTSITEETCL
T238 RTSITEETCLGQHSP
S244 ETCLGQHSPSPRPNS
S251 SPSPRPNSRSSSPGA
S253 SPRPNSRSSSPGAKR
S255 RPNSRSSSPGAKRRY
S263 PGAKRRYSCTQQPST
S271 CTQQPSTSPRQSRTP
T277 TSPRQSRTPSPQSSP
S279 PRQSRTPSPQSSPRI
S283 RTPSPQSSPRIPLRE
T328 VPTKIWKTSPDPSSM
S329 PTKIWKTSPDPSSMP
S333 WKTSPDPSSMPSHKN
S334 KTSPDPSSMPSHKNS
H338 DPSSMPSHKNSIPCH
F347 NSIPCHVFQTVDFHG
S364 EQEDRRNSAPESILL
S375 SILLVPPSWTKQLMP
T483 VHRITGKTVTTTSYE
T485 RITGKTVTTTSYEKI
I585 PMVERQDIDSCLVYG
T599 GGQQMILTGQNFTAE
A605 LTGQNFTAESKVVFT
T615 KVVFTEKTSDGQQIW
S616 VVFTEKTSDGQQIWE
T652 YRNKHIRTPVKVNFY
K682 YHPVPSIKTEPIDDY
S738 QQLRSGLSSPDSRYH
S739 QLRSGLSSPDSRYHQ
Y754 QNPAAVVYQRSKSLS
S757 AAVVYQRSKSLSPSQ
S759 VVYQRSKSLSPSQLG
S761 YQRSKSLSPSQLGYQ
S763 RSKSLSPSQLGYQQS
A778 SLMATPVAISDAHRS
S792 SVLVHTGSPGQTSAV
S810 SPGNQQSSPVIHYSP
S858 VSQAQRLSPSSYPTV
S861 AQRLSPSSYPTVIQQ
Y862 QRLSPSSYPTVIQQQ
S884 AKNGPPVSDQKEVLP
T895 EVLPAGVTIKQEQNL
K897 LPAGVTIKQEQNLDQ
Y906 EQNLDQAYLDDAHWG
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