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Protein Page:
MITF (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
MITF a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. Plays a critical role in the differentiation of various cell types including neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Two isoforms are known: the M-isoform is expressed exclusively in melanocytes, while the A-isoform has a much broader range of expression. Mutations in MITF can lead to Waardenburg syndrome. Ten alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Transcription factor; DNA binding protein
Chromosomal Location of Human Ortholog: 3p14.2-p14.1
Cellular Component: protein complex; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein dimerization activity; protein binding; chromatin binding
Biological Process: transcription from RNA polymerase II promoter; cell fate commitment; regulation of osteoclast differentiation; camera-type eye development; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; melanocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; protein complex assembly; osteoclast differentiation; bone remodeling; regulation of cell proliferation
Reference #:  O75030 (UniProtKB)
Alt. Names/Synonyms: BHLHE32; Class E basic helix-loop-helix protein 32; homolog of mouse microphthalmia; MI; Microphthalmia-associated transcription factor; microphthalmia-associated transcription factor-M transcript; MITF; WS2A
Gene Symbols: MITF
Molecular weight: 58,795 Da
Basal Isoelectric point: 5.93  Predict pI for various phosphorylation states
CST Pathways:  Growth And Differentiation Control by MAPKs
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

MITF

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 - gap
0 1 T54-p PISSSSMtSRILLRQ
0 1 S157-p KHANQVLsLPCPNQP
16 0 S180-p PGSSAPNsPMAMLTL
1 0 S280 PGLTISNSCPANLPN
3 0 K289 PANLPNIKRELTACI
1 0 K308-ub SEARALAkERQKKDN
3 1 S405-p QARAHGLsLIPSTGL
2 18 S414-p IPSTGLCsPDLVNRI
3 0 K423 DLVNRIIKQEPVLEN
0 2 T489-p EDILMDDtLsPVGVT
0 13 S491-p ILMDDtLsPVGVTDP
0 1 S502-p VTDPLLSsVsPGAsK
0 9 S504-p DPLLSsVsPGAsKts
0 2 S508-p SsVsPGAsKtssRRs
0 1 T510-p VsPGAsKtssRRssM
0 1 S511-p sPGAsKtssRRssMS
0 1 S512-p PGAsKtssRRssMSM
0 1 S515-p sKtssRRssMSMEET
8 2 S516-p KtssRRssMSMEETE
  MITF iso5  
S32-p KLTLCLFsRSSSAEH
T53 PISSSSMTSRILLRQ
S156 KHANQVLSLPCPNQP
S179 PGSSAPNSPMAMLTL
S279 PGLTISNSCPANLPN
K288 PANLPNIKRELTACI
K307 SEARALAKERQKKDN
S404 QARAHGLSLIPSTGL
S413 IPSTGLCSPDLVNRI
K422 DLVNRIIKQEPVLEN
T488 EDILMDDTLSPVGVT
S490 ILMDDTLSPVGVTDP
S501 VTDPLLSSVSPGASK
S503 DPLLSSVSPGASKTS
S507 SSVSPGASKTSSRRS
T509 VSPGASKTSSRRSSM
S510 SPGASKTSSRRSSMS
S511 PGASKTSSRRSSMSM
S514 SKTSSRRSSMSMEET
S515 KTSSRRSSMSMEETE
  MITF iso9  
- gap
- gap
S50 KHANQVLSLPCPNQP
S73-p PGSSAPNsPMAMLTL
S173 PGLTISNSCPANLPN
K182-sm PANLPNIkRELTACI
K201 SEARALAKERQKKDN
S298-p QARAHGLsLIPSTGL
S307 IPSTGLCSPDLVNRI
K316-sm DLVNRIIkQEPVLEN
T382 EDILMDDTLSPVGVT
S384 ILMDDTLSPVGVTDP
S395 VTDPLLSSVSPGASK
S397 DPLLSSVSPGASKTS
S401 SSVSPGASKTSSRRS
T403 VSPGASKTSSRRSsM
S404 SPGASKTSSRRSsMS
S405 PGASKTSSRRSsMSM
S408 SKTSSRRSsMSMEET
S409-p KTSSRRSsMSMEETE
  mouse

► Hide Isoforms
 
- gap
T54 PISSSSMTSRILLRQ
S157 KHASQVLSSPCPNQP
S180-p PGSSAPNsPMAMLTL
S280 PGLTISNSCPANLPN
K289-sm PANLPNIkRELTACI
K308 SEARALAKERQKKDN
S405-p QARAHGLsLIPSTGL
S414-p IPSTGLCsPDLVNRI
K423-sm DLVNRIIkQEPVLEN
A489 EDILMDDALsPVGVT
S491-p ILMDDALsPVGVTDP
S502 VTDPLLSSVsPGAsK
S504-p DPLLSSVsPGAsKTS
S508-p SSVsPGAsKTSSRRS
T510 VsPGAsKTSSRRSsM
S511 sPGAsKTSSRRSsMS
S512 PGAsKTSSRRSsMSA
S515 sKTSSRRSsMSAEET
S516-p KTSSRRSsMSAEETE
  MITF iso8  
- gap
- gap
S50 KHASQVLSSPCPNQP
S73-p PGSSAPNsPMAMLTL
S173-p PGLTISNsCPANLPN
K182-sm PANLPNIkRELTACI
K201 SEARALAKERQKKDN
S298 QARAHGLSLIPSTGL
S307-p IPSTGLCsPDLVNRI
K316-sm DLVNRIIkQEPVLEN
A382 EDILMDDALSPVGVT
S384 ILMDDALSPVGVTDP
S395 VTDPLLSSVSPGASK
S397 DPLLSSVSPGASKTS
S401 SSVSPGASKTSSRRS
T403 VSPGASKTSSRRSsM
S404 SPGASKTSSRRSsMS
S405 PGASKTSSRRSsMSA
S408 SKTSSRRSsMSAEET
S409-p KTSSRRSsMSAEETE
  rat

 
- gap
T54 PLSSSTMTSRILLRQ
S157 KHAGQVLSPPCPNQP
S180 PGSSAPNSPMAMLTL
S280 PGLTISNSCPANLPN
K289 PANLPNIKRELTACI
K308 SEARALAKERQKKDN
S405 QARAHGLSLIPSTGL
S414 IPSTGLCSPDLVNRI
K423 DLVNRIIKQEPVLEN
A489 EDILMDDALSPVGVT
S491 ILMDDALSPVGVTDP
S502 VTDPLLSSVSPGASK
S504 DPLLSSVSPGASKTS
S508 SSVSPGASKTSSRRS
T510 VSPGASKTSSRRSSM
S511 SPGASKTSSRRSSMS
S512 PGASKTSSRRSSMSA
S515 SKTSSRRSSMSAEET
S516 KTSSRRSSMSAEETE
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