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Protein Page:
H4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
H4 histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The gene encoding H4 is intronless. Note: This description may include information from UniProtKB.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 6p22.2
Cellular Component: extracellular region; membrane; nuclear chromosome; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleus; protein complex
Molecular Function: DNA binding; histone binding; protein binding; protein domain specific binding; protein heterodimerization activity
Biological Process: cellular protein metabolic process; chromatin silencing at rDNA; DNA replication-dependent nucleosome assembly; DNA replication-independent nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; double-strand break repair via nonhomologous end joining; negative regulation of gene expression, epigenetic; negative regulation of megakaryocyte differentiation; nucleosome assembly; positive regulation of gene expression, epigenetic; protein heterotetramerization; RNA-mediated gene silencing; telomere capping; telomere organization and biogenesis; transcription initiation
Reference #:  P62805 (UniProtKB)
Alt. Names/Synonyms: H4; H4 histone family, member A; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4F2; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; histone 1, H4a; histone cluster 1, H4a; Histone H4
Gene Symbols: HIST1H4A
Molecular weight: 11,236 Da
Basal Isoelectric point: 11.36  Predict pI for various phosphorylation states
CST Pathways:  Histone Methylation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

H4

Protein Structure Not Found.
Download PyMol Script
Download ChimeraX Script


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Sites Implicated In
cell cycle regulation: K20‑m1
DNA repair, induced: Y72‑p
methylation: Y72‑p
molecular association, regulation: K20‑m1, Y72‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 1 S1‑p _______sGrGkGGk
5 0 R3‑me _____sGrGkGGkGL
3 0 R3‑m1 _____sGrGkGGkGL
7 0 R3‑m2 _____sGrGkGGkGL
54 508 K5‑ac ___sGrGkGGkGLGk
63 521 K8‑ac sGrGkGGkGLGkGGA
67 527 K12‑ac kGGkGLGkGGAkRHR
0 2 K12‑m3 kGGkGLGkGGAkRHR
0 2 K12‑ub kGGkGLGkGGAkRHR
1 0 K12‑sm kGGkGLGkGGAkRHR
0 1 K12‑sc kGGkGLGkGGAkRHR
78 528 K16‑ac GLGkGGAkRHRkVLR
0 3 K16‑m3 GLGkGGAkRHRkVLR
1 0 H18 GkGGAkRHRkVLRDN
1 1 K20‑ac GGAkRHRkVLRDNIQ
5 0 K20‑me GGAkRHRkVLRDNIQ
27 8 K20‑m1 GGAkRHRkVLRDNIQ
25 6 K20‑m2 GGAkRHRkVLRDNIQ
35 2 K20‑m3 GGAkRHRkVLRDNIQ
0 1 R23 kRHRkVLRDNIQGIT
0 1 R23 kRHRkVLRDNIQGIT
0 10 K31‑ac DNIQGITkPAIRRLA
0 1 K31‑m1 DNIQGITkPAIRRLA
1 1 K31‑m2 DNIQGITkPAIRRLA
0 130 K31‑ub DNIQGITkPAIRRLA
0 2 K31‑sc DNIQGITkPAIRRLA
0 1 R35 GITkPAIRRLARRGG
3 56 S47‑p RGGVKRIsGLIyEEt
0 700 Y51‑p KRIsGLIyEEtrGVL
0 8 T54‑p sGLIyEEtrGVLkVF
1 0 R55‑me GLIyEEtrGVLkVFL
1 0 R55‑m2 GLIyEEtrGVLkVFL
0 36 K59‑ac EEtrGVLkVFLENVI
0 1 K59‑m1 EEtrGVLkVFLENVI
0 1 K59‑m2 EEtrGVLkVFLENVI
0 1 K59‑m3 EEtrGVLkVFLENVI
0 63 K59‑ub EEtrGVLkVFLENVI
1 147 Y72‑p VIRDAVTyTEHAkRk
1 0 H75 DAVTyTEHAkRktVt
1 97 K77‑ac VTyTEHAkRktVtAM
1 0 K77‑me VTyTEHAkRktVtAM
0 38 K77‑ub VTyTEHAkRktVtAM
0 2 K77‑sc VTyTEHAkRktVtAM
0 2 K79‑ac yTEHAkRktVtAMDV
0 3 K79‑ub yTEHAkRktVtAMDV
0 1 K79 yTEHAkRKtVtAMDV
1 12 T80‑p TEHAkRktVtAMDVV
0 5 T82‑p HAkRktVtAMDVVyA
1 1043 Y88‑p VtAMDVVyALkRQGR
4 242 K91‑ac MDVVyALkRQGRTLy
2 17 K91‑ub MDVVyALkRQGRTLy
0 2 K91‑sc MDVVyALkRQGRTLy
0 4 Y98‑p kRQGRTLyGFGG___
8647 : Acetyl-Histone H4 (Lys5) (D12B3) Rabbit mAb
9672 : Acetyl-Histone H4 (Lys5) Antibody
2594 : Acetyl-Histone H4 (Lys8) Antibody
2591 : Acetyl-Histone H4 (Lys12) Antibody
13944 : Acetyl-Histone H4 (Lys12) (D2W6O) Rabbit mAb
8804 : Acetyl-Histone H4 (Lys16) Antibody
13534 : Acetyl-Histone H4 (Lys16) (E2B8W) Rabbit mAb
9724 : Mono-Methyl-Histone H4 (Lys20) Antibody
9759 :
5737 : Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb
  mouse

 
S1 _______SGrGkGGk
R3 _____SGRGkGGkGL
R3 _____SGRGkGGkGL
R3‑m2 _____SGrGkGGkGL
K5‑ac ___SGrGkGGkGLGk
K8‑ac SGrGkGGkGLGkGGA
K12‑ac kGGkGLGkGGAkRHR
K12 kGGkGLGKGGAkRHR
K12 kGGkGLGKGGAkRHR
K12 kGGkGLGKGGAkRHR
K12 kGGkGLGKGGAkRHR
K16‑ac GLGkGGAkRHRkVLr
K16‑m3 GLGkGGAkRHRkVLr
H18 GkGGAkRHRkVLrDN
K20‑ac GGAkRHRkVLrDNIQ
K20 GGAkRHRKVLrDNIQ
K20‑m1 GGAkRHRkVLrDNIQ
K20‑m2 GGAkRHRkVLrDNIQ
K20‑m3 GGAkRHRkVLrDNIQ
R23‑m1 kRHRkVLrDNIQGIT
R23‑m2 kRHRkVLrDNIQGIT
K31‑ac DNIQGITkPAIrRLA
K31 DNIQGITKPAIrRLA
K31 DNIQGITKPAIrRLA
K31‑ub DNIQGITkPAIrRLA
K31‑sc DNIQGITkPAIrRLA
R35‑m1 GITkPAIrRLARRGG
S47‑p RGGVKRIsGLIyEET
Y51‑p KRIsGLIyEETRGVL
T54 sGLIyEETRGVLkVF
R55 GLIyEETRGVLkVFL
R55 GLIyEETRGVLkVFL
K59 EETRGVLKVFLENVI
K59 EETRGVLKVFLENVI
K59 EETRGVLKVFLENVI
K59 EETRGVLKVFLENVI
K59‑ub EETRGVLkVFLENVI
Y72‑p VIRDAVTyTEHAkRk
H75 DAVTyTEHAkRktVT
K77‑ac VTyTEHAkRktVTAM
K77 VTyTEHAKRktVTAM
K77‑ub VTyTEHAkRktVTAM
K77‑sc VTyTEHAkRktVTAM
K79 yTEHAkRKtVTAMDV
K79 yTEHAkRKtVTAMDV
K79‑sc yTEHAkRktVTAMDV
T80‑p TEHAkRktVTAMDVV
T82 HAkRktVTAMDVVyA
Y88‑p VTAMDVVyALkRQGR
K91‑ac MDVVyALkRQGRTLY
K91‑ub MDVVyALkRQGRTLY
K91‑sc MDVVyALkRQGRTLY
Y98 kRQGRTLYGFGG___
8647 : Acetyl-Histone H4 (Lys5) (D12B3) Rabbit mAb
9672 : Acetyl-Histone H4 (Lys5) Antibody
2594 : Acetyl-Histone H4 (Lys8) Antibody
2591 : Acetyl-Histone H4 (Lys12) Antibody
13944 : Acetyl-Histone H4 (Lys12) (D2W6O) Rabbit mAb
8804 : Acetyl-Histone H4 (Lys16) Antibody
13534 : Acetyl-Histone H4 (Lys16) (E2B8W) Rabbit mAb
9724 : Mono-Methyl-Histone H4 (Lys20) Antibody
9759 :
5737 : Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb
  rat

 
S1 _______SGRGkGGk
R3 _____SGRGkGGkGL
R3 _____SGRGkGGkGL
R3 _____SGRGkGGkGL
K5‑ac ___SGRGkGGkGLGk
K8‑ac SGRGkGGkGLGkGGA
K12‑ac kGGkGLGkGGAkRHR
K12 kGGkGLGKGGAkRHR
K12 kGGkGLGKGGAkRHR
K12 kGGkGLGKGGAkRHR
K12 kGGkGLGKGGAkRHR
K16‑ac GLGkGGAkRHRKVLR
K16 GLGkGGAKRHRKVLR
H18 GkGGAkRHRKVLRDN
K20 GGAkRHRKVLRDNIQ
K20 GGAkRHRKVLRDNIQ
K20 GGAkRHRKVLRDNIQ
K20 GGAkRHRKVLRDNIQ
K20 GGAkRHRKVLRDNIQ
R23 kRHRKVLRDNIQGIT
R23 kRHRKVLRDNIQGIT
K31‑ac DNIQGITkPAIRRLA
K31 DNIQGITKPAIRRLA
K31 DNIQGITKPAIRRLA
K31 DNIQGITKPAIRRLA
K31 DNIQGITKPAIRRLA
R35 GITkPAIRRLARRGG
S47‑p RGGVKRIsGLIyEET
Y51‑p KRIsGLIyEETRGVL
T54 sGLIyEETRGVLkVF
R55 GLIyEETRGVLkVFL
R55 GLIyEETRGVLkVFL
K59‑ac EETRGVLkVFLENVI
K59 EETRGVLKVFLENVI
K59 EETRGVLKVFLENVI
K59 EETRGVLKVFLENVI
K59 EETRGVLKVFLENVI
Y72 VIRDAVTYTEHAkRk
H75 DAVTYTEHAkRktVT
K77‑ac VTYTEHAkRktVTAM
K77 VTYTEHAKRktVTAM
K77 VTYTEHAKRktVTAM
K77 VTYTEHAKRktVTAM
K79‑ac YTEHAkRktVTAMDV
K79 YTEHAkRKtVTAMDV
K79 YTEHAkRKtVTAMDV
T80‑p TEHAkRktVTAMDVV
T82 HAkRktVTAMDVVyA
Y88‑p VTAMDVVyALkRQGR
K91‑ac MDVVyALkRQGRTLY
K91 MDVVyALKRQGRTLY
K91 MDVVyALKRQGRTLY
Y98 kRQGRTLYGFGG___
8647 : Acetyl-Histone H4 (Lys5) (D12B3) Rabbit mAb
9672 : Acetyl-Histone H4 (Lys5) Antibody
2591 : Acetyl-Histone H4 (Lys12) Antibody
13944 : Acetyl-Histone H4 (Lys12) (D2W6O) Rabbit mAb
8804 : Acetyl-Histone H4 (Lys16) Antibody
13534 : Acetyl-Histone H4 (Lys16) (E2B8W) Rabbit mAb
5737 : Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb
  pig

 
S1 _______SGRGKGGk
R3 _____SGRGKGGkGL
R3 _____SGRGKGGkGL
R3 _____SGRGKGGkGL
K5 ___SGRGKGGkGLGK
K8‑ac SGRGKGGkGLGKGGA
K12 KGGkGLGKGGAKRHR
K12 KGGkGLGKGGAKRHR
K12 KGGkGLGKGGAKRHR
K12 KGGkGLGKGGAKRHR
K12 KGGkGLGKGGAKRHR
K16 GLGKGGAKRHRKVLR
K16 GLGKGGAKRHRKVLR
H18 GKGGAKRHRKVLRDN
K20 GGAKRHRKVLRDNIQ
K20 GGAKRHRKVLRDNIQ
K20 GGAKRHRKVLRDNIQ
K20 GGAKRHRKVLRDNIQ
K20 GGAKRHRKVLRDNIQ
R23 KRHRKVLRDNIQGIT
R23 KRHRKVLRDNIQGIT
K31 DNIQGITKPAIRRLA
K31 DNIQGITKPAIRRLA
K31 DNIQGITKPAIRRLA
K31 DNIQGITKPAIRRLA
K31 DNIQGITKPAIRRLA
R35 GITKPAIRRLARRGG
S47 RGGVKRISGLIYEET
Y51 KRISGLIYEETRGVL
T54 SGLIYEETRGVLKVF
R55 GLIYEETRGVLKVFL
R55 GLIYEETRGVLKVFL
K59 EETRGVLKVFLENVI
K59 EETRGVLKVFLENVI
K59 EETRGVLKVFLENVI
K59 EETRGVLKVFLENVI
K59 EETRGVLKVFLENVI
Y72 VIRDAVTYTEHAKRK
H75 DAVTYTEHAKRKTVT
K77 VTYTEHAKRKTVTAM
K77 VTYTEHAKRKTVTAM
K77 VTYTEHAKRKTVTAM
K77 VTYTEHAKRKTVTAM
K79 YTEHAKRKTVTAMDV
K79 YTEHAKRKTVTAMDV
K79 YTEHAKRKTVTAMDV
T80 TEHAKRKTVTAMDVV
T82 HAKRKTVTAMDVVYA
Y88 VTAMDVVYALKRQGR
K91 MDVVYALKRQGRTLY
K91 MDVVYALKRQGRTLY
K91 MDVVYALKRQGRTLY
Y98 KRQGRTLYGFGG___
8647 : Acetyl-Histone H4 (Lys5) (D12B3) Rabbit mAb
9672 : Acetyl-Histone H4 (Lys5) Antibody
2591 : Acetyl-Histone H4 (Lys12) Antibody
13944 : Acetyl-Histone H4 (Lys12) (D2W6O) Rabbit mAb
8804 : Acetyl-Histone H4 (Lys16) Antibody
13534 : Acetyl-Histone H4 (Lys16) (E2B8W) Rabbit mAb
5737 : Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb
  cow

 
S1‑p _______sGRGkGGk
R3 _____sGRGkGGkGL
R3 _____sGRGkGGkGL
R3 _____sGRGkGGkGL
K5‑ac ___sGRGkGGkGLGk
K8‑ac sGRGkGGkGLGkGGA
K12‑ac kGGkGLGkGGAkRhR
K12 kGGkGLGKGGAkRhR
K12 kGGkGLGKGGAkRhR
K12 kGGkGLGKGGAkRhR
K12 kGGkGLGKGGAkRhR
K16‑ac GLGkGGAkRhRKVLR
K16 GLGkGGAKRhRKVLR
H18‑p GkGGAkRhRKVLRDN
K20 GGAkRhRKVLRDNIQ
K20 GGAkRhRKVLRDNIQ
K20 GGAkRhRKVLRDNIQ
K20 GGAkRhRKVLRDNIQ
K20 GGAkRhRKVLRDNIQ
R23 kRhRKVLRDNIQGIT
R23 kRhRKVLRDNIQGIT
K31 DNIQGITKPAIRRLA
K31 DNIQGITKPAIRRLA
K31 DNIQGITKPAIRRLA
K31 DNIQGITKPAIRRLA
K31 DNIQGITKPAIRRLA
R35 GITKPAIRRLARRGG
S47‑p RGGVKRIsGLIYEET
Y51 KRIsGLIYEETRGVL
T54 sGLIYEETRGVLKVF
R55 GLIYEETRGVLKVFL
R55 GLIYEETRGVLKVFL
K59 EETRGVLKVFLENVI
K59 EETRGVLKVFLENVI
K59 EETRGVLKVFLENVI
K59 EETRGVLKVFLENVI
K59 EETRGVLKVFLENVI
Y72 VIRDAVTYTEhAKRK
H75‑p DAVTYTEhAKRKTVT
K77 VTYTEhAKRKTVTAM
K77 VTYTEhAKRKTVTAM
K77 VTYTEhAKRKTVTAM
K77 VTYTEhAKRKTVTAM
K79 YTEhAKRKTVTAMDV
K79 YTEhAKRKTVTAMDV
K79 YTEhAKRKTVTAMDV
T80 TEhAKRKTVTAMDVV
T82 hAKRKTVTAMDVVYA
Y88 VTAMDVVYALKRQGR
K91 MDVVYALKRQGRTLY
K91 MDVVYALKRQGRTLY
K91 MDVVYALKRQGRTLY
Y98 KRQGRTLYGFGG___
8647 : Acetyl-Histone H4 (Lys5) (D12B3) Rabbit mAb
9672 : Acetyl-Histone H4 (Lys5) Antibody
2591 : Acetyl-Histone H4 (Lys12) Antibody
13944 : Acetyl-Histone H4 (Lys12) (D2W6O) Rabbit mAb
8804 : Acetyl-Histone H4 (Lys16) Antibody
13534 : Acetyl-Histone H4 (Lys16) (E2B8W) Rabbit mAb
9724 : Mono-Methyl-Histone H4 (Lys20) Antibody
9759 :
5737 : Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb
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