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Protein Page:
H2B (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
H2B histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The gene encoding H2B is intronless. Note: This description may include information from UniProtKB.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: cytoplasm; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleus
Molecular Function: DNA binding; protein binding; protein heterodimerization activity
Biological Process: nucleosome assembly
Reference #:  P33778 (UniProtKB)
Gene Symbols: HIST1H2BB
Molecular weight: 13,819 Da
Basal Isoelectric point: 10.32  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

H2B

Protein Structure Not Found.
Download PyMol Script
Download ChimeraX Script


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Sites Implicated In
cell growth, induced: S32‑p
translation, induced: S32‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
5 120 K5‑ac ___PEPSksAPAPkk
0 10 K5‑ub ___PEPSksAPAPkk
0 1 S6‑p __PEPSksAPAPkkG
1 427 K11‑ac SksAPAPkkGskkAI
5 460 K12‑ac ksAPAPkkGskkAIt
9 1 S14‑p APAPkkGskkAItkA
6 458 K15‑ac PAPkkGskkAItkAQ
0 1 K15‑m3 PAPkkGskkAItkAQ
2 324 K16‑ac APkkGskkAItkAQk
0 1 T19‑p kGskkAItkAQkkDG
3 236 K20‑ac GskkAItkAQkkDGK
0 72 K23‑ac kAItkAQkkDGKkRK
0 1 K23‑m2 kAItkAQkkDGKkRK
0 1 K23‑m3 kAItkAQkkDGKkRK
1 8 K24‑ac AItkAQkkDGKkRKR
0 1 K28‑ac AQkkDGKkRKRsRkE
7 0 S32‑p DGKkRKRsRkEsysI
1 4 K34‑ub KkRKRsRkEsysIyV
6 58 S36‑p RKRsRkEsysIyVyk
0 10 Y37‑p KRsRkEsysIyVykV
1 10 S38‑p RsRkEsysIyVykVL
0 99 Y40‑p RkEsysIyVykVLkQ
0 137 Y42‑p EsysIyVykVLkQVH
0 3 K43‑ac sysIyVykVLkQVHP
0 1 K43‑m3 sysIyVykVLkQVHP
0 5 K43‑ub sysIyVykVLkQVHP
1 6 K46‑ac IyVykVLkQVHPDTG
1 0 K46‑me IyVykVLkQVHPDTG
0 1 K46‑m2 IyVykVLkQVHPDTG
0 130 K46‑ub IyVykVLkQVHPDTG
0 4 S55‑p VHPDTGIsskAMGIM
0 4 S56‑p HPDTGIsskAMGIMN
0 4 K57‑ac PDTGIsskAMGIMNs
1 0 K57‑m2 PDTGIsskAMGIMNs
0 23 K57‑ub PDTGIsskAMGIMNs
0 57 S64‑p kAMGIMNsFVNDIFE
0 2 G75 DIFERIAGEASRLAH
0 73 Y83‑p EASRLAHyNkRstIt
0 18 K85‑ac SRLAHyNkRstItsR
0 43 K85‑ub SRLAHyNkRstItsR
0 7 S87‑p LAHyNkRstItsREI
0 3 T88‑p AHyNkRstItsREIQ
0 4 T90‑p yNkRstItsREIQTA
0 3 S91‑p NkRstItsREIQTAV
0 22 K108‑ac LLPGELAkHAVsEGt
1 0 K108‑me LLPGELAkHAVsEGt
0 1 K108‑m2 LLPGELAkHAVsEGt
0 130 K108‑ub LLPGELAkHAVsEGt
0 10 S112‑p ELAkHAVsEGtkAVt
1 0 S112‑gl ELAkHAVsEGtkAVt
0 4 T115‑p kHAVsEGtkAVtkYT
1 9 K116‑ac HAVsEGtkAVtkYTS
0 1 K116‑me HAVsEGtkAVtkYTS
0 135 K116‑ub HAVsEGtkAVtkYTS
1 3 T119‑p sEGtkAVtkYTSSk_
2 19 K120‑ac EGtkAVtkYTSSk__
0 1 K120‑m3 EGtkAVtkYTSSk__
10 146 K120‑ub EGtkAVtkYTSSk__
0 13 K125‑ac VtkYTSSk_______
2 1 K125‑ub VtkYTSSk_______
2574 : Acetyl-Histone H2B (Lys5) Antibody
12799 : Acetyl-Histone H2B (Lys5) (D5H1S) XP(R) Rabbit mAb
5410 : Acetyl-Histone H2B (Lys12) Antibody
9072 : Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated)
9861 : Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb
6959 : Phospho-Histone H2B (Ser14) (D67H2) Rabbit mAb
5435 : Acetyl-Histone H2B (Lys15) Antibody
9083 : Acetyl-Histone H2B (Lys15) (D8H1) XP(R) Rabbit mAb
2571 : Acetyl-Histone H2B (Lys20) Antibody
5546 : Ubiquityl-Histone H2B (Lys120) (D11) XP(R) Rabbit mAb
  mouse

 
K5‑ac ___PEPAkSAPAPkk
K5‑ub ___PEPAkSAPAPkk
S6 __PEPAkSAPAPkkG
K11‑ac AkSAPAPkkGskkAV
K12‑ac kSAPAPkkGskkAVT
S14‑p APAPkkGskkAVTkA
K15‑ac PAPkkGskkAVTkAQ
K15 PAPkkGsKkAVTkAQ
K16‑ac APkkGskkAVTkAQk
T19 kGskkAVTkAQkkDG
K20‑ac GskkAVTkAQkkDGK
K23‑ac kAVTkAQkkDGKKRK
K23 kAVTkAQKkDGKKRK
K23 kAVTkAQKkDGKKRK
K24‑ac AVTkAQkkDGKKRKR
K28 AQkkDGKKRKRsRkE
S32‑p DGKKRKRsRkEsysV
K34‑ub KKRKRsRkEsysVyV
S36‑p RKRsRkEsysVyVyk
Y37‑p KRsRkEsysVyVykV
S38‑p RsRkEsysVyVykVL
Y40‑p RkEsysVyVykVLkQ
Y42‑p EsysVyVykVLkQVH
K43‑ac sysVyVykVLkQVHP
K43 sysVyVyKVLkQVHP
K43‑ub sysVyVykVLkQVHP
K46‑ac VyVykVLkQVHPDTG
K46 VyVykVLKQVHPDTG
K46 VyVykVLKQVHPDTG
K46‑ub VyVykVLkQVHPDTG
S55‑p VHPDTGIsskAMGIM
S56‑p HPDTGIsskAMGIMN
K57 PDTGIssKAMGIMNs
K57 PDTGIssKAMGIMNs
K57‑ub PDTGIsskAMGIMNs
S64‑p kAMGIMNsFVNDIFE
S75‑p DIFERIAsEASRLAH
Y83‑p EASRLAHyNkRstIT
K85‑ac SRLAHyNkRstITsR
K85‑ub SRLAHyNkRstITsR
S87‑p LAHyNkRstITsREI
T88‑p AHyNkRstITsREIQ
T90 yNkRstITsREIQTA
S91‑p NkRstITsREIQTAV
K108‑ac LLPGELAkHAVsEGt
K108 LLPGELAKHAVsEGt
K108 LLPGELAKHAVsEGt
K108‑ub LLPGELAkHAVsEGt
S112‑p ELAkHAVsEGtkAVt
S112 ELAkHAVSEGtkAVt
T115‑p kHAVsEGtkAVtkYT
K116 HAVsEGtKAVtkYTS
K116 HAVsEGtKAVtkYTS
K116‑ub HAVsEGtkAVtkYTS
T119‑p sEGtkAVtkYTSSk_
K120‑ac EGtkAVtkYTSSk__
K120 EGtkAVtKYTSSk__
K120‑ub EGtkAVtkYTSSk__
K125‑ac VtkYTSSk_______
K125 VtkYTSSK_______
2574 : Acetyl-Histone H2B (Lys5) Antibody
12799 : Acetyl-Histone H2B (Lys5) (D5H1S) XP(R) Rabbit mAb
5410 : Acetyl-Histone H2B (Lys12) Antibody
9072 : Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated)
9861 : Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb
6959 : Phospho-Histone H2B (Ser14) (D67H2) Rabbit mAb
5435 : Acetyl-Histone H2B (Lys15) Antibody
9083 : Acetyl-Histone H2B (Lys15) (D8H1) XP(R) Rabbit mAb
2571 : Acetyl-Histone H2B (Lys20) Antibody
5546 : Ubiquityl-Histone H2B (Lys120) (D11) XP(R) Rabbit mAb
  rat

 
R5 ___PEPSRSTPAPkk
R5 ___PEPSRSTPAPkk
S6 __PEPSRSTPAPkkG
K11‑ac SRSTPAPkkGskkAI
K12‑ac RSTPAPkkGskkAIT
S14‑p TPAPkkGskkAITkA
K15‑ac PAPkkGskkAITkAQ
K15 PAPkkGsKkAITkAQ
K16‑ac APkkGskkAITkAQK
T19 kGskkAITkAQKKDG
K20‑ac GskkAITkAQKKDGK
K23 kAITkAQKKDGKKRK
K23 kAITkAQKKDGKKRK
K23 kAITkAQKKDGKKRK
K24 AITkAQKKDGKKRKR
K28 AQKKDGKKRKRGRKE
G32 DGKKRKRGRKESYSI
K34 KKRKRGRKESYSIYV
S36 RKRGRKESYSIYVYK
Y37 KRGRKESYSIYVYKV
S38 RGRKESYSIYVYKVL
Y40 RKESYSIYVYKVLkQ
Y42 ESYSIYVYKVLkQVH
K43 SYSIYVYKVLkQVHP
K43 SYSIYVYKVLkQVHP
K43 SYSIYVYKVLkQVHP
K46‑ac IYVYKVLkQVHPDTG
K46 IYVYKVLKQVHPDTG
K46 IYVYKVLKQVHPDTG
K46‑ub IYVYKVLkQVHPDTG
S55 VHPDTGISSKAMGIM
S56 HPDTGISSKAMGIMN
K57 PDTGISSKAMGIMNs
K57 PDTGISSKAMGIMNs
K57 PDTGISSKAMGIMNs
S64‑p KAMGIMNsFVNDIFE
S75 DIFERIASEASRLAH
Y83 EASRLAHYNkRSTIT
K85‑ac SRLAHYNkRSTITSR
K85 SRLAHYNKRSTITSR
S87 LAHYNkRSTITSREV
T88 AHYNkRSTITSREVQ
T90 YNkRSTITSREVQTA
S91 NkRSTITSREVQTAV
K108‑ac LLPGELAkHAVsEGT
K108 LLPGELAKHAVsEGT
K108 LLPGELAKHAVsEGT
K108‑ub LLPGELAkHAVsEGT
S112‑p ELAkHAVsEGTkAVT
S112 ELAkHAVSEGTkAVT
T115 kHAVsEGTkAVTkYT
K116‑ac HAVsEGTkAVTkYTS
K116 HAVsEGTKAVTkYTS
K116‑ub HAVsEGTkAVTkYTS
T119 sEGTkAVTkYTSSK_
K120‑ac EGTkAVTkYTSSK__
K120 EGTkAVTKYTSSK__
K120‑ub EGTkAVTkYTSSK__
K125 VTkYTSSK_______
K125 VTkYTSSK_______
2574 : Acetyl-Histone H2B (Lys5) Antibody
12799 : Acetyl-Histone H2B (Lys5) (D5H1S) XP(R) Rabbit mAb
5410 : Acetyl-Histone H2B (Lys12) Antibody
9072 : Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated)
9861 : Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb
6959 : Phospho-Histone H2B (Ser14) (D67H2) Rabbit mAb
5435 : Acetyl-Histone H2B (Lys15) Antibody
9083 : Acetyl-Histone H2B (Lys15) (D8H1) XP(R) Rabbit mAb
5546 : Ubiquityl-Histone H2B (Lys120) (D11) XP(R) Rabbit mAb
  cow

 
K5 ___PEPAKSAPAPKK
K5 ___PEPAKSAPAPKK
S6 __PEPAKSAPAPKKG
K11 AKSAPAPKKGSKKAV
K12 KSAPAPKKGSKKAVT
S14 APAPKKGSKKAVTKA
K15 PAPKKGSKKAVTKAQ
K15 PAPKKGSKKAVTKAQ
K16 APKKGSKKAVTKAQK
T19 KGSKKAVTKAQKKDG
K20 GSKKAVTKAQKKDGK
K23 KAVTKAQKKDGKKRK
K23 KAVTKAQKKDGKKRK
K23 KAVTKAQKKDGKKRK
K24 AVTKAQKKDGKKRKR
K28 AQKKDGKKRKRsRKE
S32‑p DGKKRKRsRKEsYSV
K34 KKRKRsRKEsYSVYV
S36‑p RKRsRKEsYSVYVYK
Y37 KRsRKEsYSVYVYKV
S38 RsRKEsYSVYVYKVL
Y40 RKEsYSVYVYKVLKQ
Y42 EsYSVYVYKVLKQVH
K43 sYSVYVYKVLKQVHP
K43 sYSVYVYKVLKQVHP
K43 sYSVYVYKVLKQVHP
K46 VYVYKVLKQVHPDTG
K46 VYVYKVLKQVHPDTG
K46 VYVYKVLKQVHPDTG
K46 VYVYKVLKQVHPDTG
S55 VHPDTGISSKAMGIM
S56 HPDTGISSKAMGIMN
K57 PDTGISSKAMGIMNS
K57 PDTGISSKAMGIMNS
K57 PDTGISSKAMGIMNS
S64 KAMGIMNSFVNDIFE
G75 DIFERIAGEASRLAH
Y83 EASRLAHYNKRSTIT
K85 SRLAHYNKRSTITSR
K85 SRLAHYNKRSTITSR
S87 LAHYNKRSTITSREI
T88 AHYNKRSTITSREIQ
T90 YNKRSTITSREIQTA
S91 NKRSTITSREIQTAV
K108 LLPGELAKHAVSEGT
K108 LLPGELAKHAVSEGT
K108 LLPGELAKHAVSEGT
K108 LLPGELAKHAVSEGT
S112 ELAKHAVSEGTKAVT
S112 ELAKHAVSEGTKAVT
T115 KHAVSEGTKAVTKYT
K116 HAVSEGTKAVTKYTS
K116 HAVSEGTKAVTKYTS
K116 HAVSEGTKAVTKYTS
T119 SEGTKAVTKYTSSK_
K120 EGTKAVTKYTSSK__
K120 EGTKAVTKYTSSK__
K120 EGTKAVTKYTSSK__
K125 VTKYTSSK_______
K125 VTKYTSSK_______
5410 : Acetyl-Histone H2B (Lys12) Antibody
9072 : Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated)
9861 : Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb
6959 : Phospho-Histone H2B (Ser14) (D67H2) Rabbit mAb
5435 : Acetyl-Histone H2B (Lys15) Antibody
9083 : Acetyl-Histone H2B (Lys15) (D8H1) XP(R) Rabbit mAb
5546 : Ubiquityl-Histone H2B (Lys120) (D11) XP(R) Rabbit mAb
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