Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Heterotrimer composed of RPA1, RPA2 and RPA3 (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the RPA1 subunit. Interacts with RIPK1 and XPA. Interacts with RPA4. Interacts with the polymerase alpha subunit POLA1/p180; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interact with RAD51 and SENP6 to regulate DNA repair. Interacts with HELB; this interaction promotes HELB recruitement to chromatin following DNA damage. Belongs to the replication factor A protein 1 family. Note: This description may include information from UniProtKB.
Cellular Component: DNA replication factor A complex; lateral element; male germ cell nucleus; nuclear chromosome, telomeric region; nucleoplasm; nucleus; PML body
Molecular Function: chromatin binding; damaged DNA binding; metal ion binding; protein binding; single-stranded DNA binding
Biological Process: base-excision repair; bypass DNA synthesis; cellular protein metabolic process; DNA damage response, detection of DNA damage; DNA recombination; DNA repair; DNA replication; DNA strand elongation during DNA replication; DNA-dependent DNA replication; double-strand break repair; double-strand break repair via homologous recombination; error-prone postreplication DNA repair; G1/S transition of mitotic cell cycle; hemopoiesis; homeostasis of number of cells within a tissue; in utero embryonic development; meiosis; mismatch repair; mitotic cell cycle; nucleotide-excision repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; positive regulation of cell proliferation; post-translational protein modification; protein sumoylation; telomere maintenance; telomere maintenance via recombination; telomere maintenance via semi-conservative replication; transcription-coupled nucleotide-excision repair
Alt. Names/Synonyms: HSSB; MST075; MSTP075; REPA1; Replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit; replication protein A1; replication protein A1, 70kDa; RF-A; RF-A protein 1; RFA1; RP-A; RP-A p70; RPA1; RPA70; Single-stranded DNA-binding protein
LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.