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Protein Page:
PYCARD (human)
rdtyret
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PYCARD Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T- cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Tumor suppressor; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; IkappaB kinase complex; mitochondrion; nucleolus; nucleus
Molecular Function: caspase activator activity; enzyme binding; identical protein binding; interleukin-6 receptor binding; myosin I binding; protease binding; protein binding; protein homodimerization activity; Pyrin domain binding; tropomyosin binding
Biological Process: activation of innate immune response; activation of NF-kappaB transcription factor; apoptosis; caspase activation; defense response to Gram-negative bacterium; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; inflammatory response; inhibition of NF-kappaB transcription factor; innate immune response; myeloid dendritic cell activation; myeloid dendritic cell activation during immune response; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interferon-beta production; positive regulation of actin filament polymerization; positive regulation of activated T cell proliferation; positive regulation of adaptive immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of interferon-gamma production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-6 production; positive regulation of JNK cascade; positive regulation of phagocytosis; positive regulation of T cell activation; positive regulation of transcription factor activity; positive regulation of tumor necrosis factor production; regulation of protein stability; signal transduction; stimulatory C-type lectin receptor signaling pathway; tumor necrosis factor-mediated signaling pathway
Reference #:  Q9ULZ3 (UniProtKB)
Alt. Names/Synonyms: Apoptosis-associated speck-like protein containing a CARD; ASC; CARD5; caspase recruitment domain protein 5; Caspase recruitment domain-containing protein 5; hASC; MGC10332; PYCARD; PYD and CARD domain containing; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1; target of methylation-induced silencing-1; TMS; TMS-1; TMS1
Gene Symbols: PYCARD
Molecular weight: 21,627 Da
Basal Isoelectric point: 5.95  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PYCARD

Protein Structure Not Found.


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Modification Sites and Domains  
Click here to view other types of protein modifications

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 7 K21‑ac NLTAEELkkFKLKLL
0 2 K21‑ub NLTAEELkkFKLKLL
0 1 K22 LTAEELkKFKLKLLS
0 2 K22‑ub LTAEELkkFKLKLLS
0 1 K26 ELkkFKLKLLSVPLR
0 1 S46‑p IPRGALLsMDALDLT
0 1 K109‑ub APPQSAAkPGLHFID
0 1 K139‑ub LLDALYGkVLTDEQy
1 3 Y146‑p kVLTDEQyQAVRAEP
0 1 R182 DLLLQALRESQSYLV
0 9 S195 LVEDLERS_______
  mouse

 
K21‑ac NLSGDELkKFKMKLL
K21 NLSGDELKKFKMKLL
K22 LSGDELkKFKMKLLT
K22 LSGDELkKFKMKLLT
K26 ELkKFKMKLLTVQLR
Q46 IPRGALLQMDAIDLT
R108 VPAQSTARTGHFVDQ
S137 VLDALHGSVLTEGQy
Y144‑p SVLTEGQyQAVRAET
K180‑ub DSLLQALkEIHPYLV
S193‑p LVMDLEQs_______
  rat

 
K21‑ac NLTADEFkkFKMkLL
K21 NLTADEFKkFKMkLL
K22‑ac LTADEFkkFKMkLLT
K22 LTADEFkKFKMkLLT
K26‑ac EFkkFKMkLLTAPVR
Q46 IPRGALLQMDPIDLT
R108 VPAQGTARTEHFVDQ
N137 LLDALYGNVLTEGQY
Y144 NVLTEGQYQAVRAET
R180 NLFLEALRQTQPYLV
S193‑p LVTDLEQs_______
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