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Protein Page:
BAD (mouse)
rdtyret
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
BAD a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL. Note: This description may include information from UniProtKB.
Protein type: Apoptosis
Cellular Component: cytoplasm; cytosol; membrane; mitochondrial outer membrane; mitochondrion
Molecular Function: caspase activator activity; lipid binding; phospholipid binding; protein binding; protein heterodimerization activity; protein kinase B binding; protein kinase binding; protein phosphatase 2B binding; protein phosphatase binding
Biological Process: ADP metabolic process; apoptosis; ATP metabolic process; caspase activation; cell proliferation; cellular process regulating host cell cycle in response to virus; cytokine and chemokine mediated signaling pathway; DNA damage response, signal transduction resulting in induction of apoptosis; glucose catabolic process; glucose homeostasis; induction of apoptosis via death domain receptors; negative regulation of cytolysis; pore complex biogenesis; positive regulation of apoptosis; positive regulation of apoptosis by virus; positive regulation of B cell differentiation; positive regulation of caspase activity; positive regulation of epithelial cell proliferation; positive regulation of glucokinase activity; positive regulation of insulin secretion; positive regulation of proteolysis; positive regulation of T cell differentiation; regulation of apoptosis; regulation of caspase activity; regulation of mitochondrial membrane permeability; release of cytochrome c from mitochondria; suppression by virus of host apoptosis
Reference #:  Q61337 (UniProtKB)
Alt. Names/Synonyms: AI325008; BAD; Bbc2; Bbc6; Bcl-2-binding component 6; Bcl-associated death promoter; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; BCL2-associated agonist of cell death; OTTMUSP00000017561
Gene Symbols: Bad
Molecular weight: 22,080 Da
Basal Isoelectric point: 9.15  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  ErbB/HER Signaling  |  Growth And Differentiation Control by MAPKs  |  Inhibition of Apoptosis  |  Insulin Receptor Signaling  |  Mitochondrial Control of Apoptosis  |  PI3K/Akt Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

BAD

Protein Structure Not Found.


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Sites Implicated In
apoptosis, altered: S112‑p, S136‑p, S155‑p
apoptosis, induced: S128‑p
apoptosis, inhibited: S26‑p, S112‑p, S136‑p, S155‑p, S170‑p, T201‑p
cell cycle regulation: S170‑p
cell growth, altered: S136‑p
cell growth, inhibited: S170‑p
activity, induced: S112‑p, S136‑p, T201‑p
activity, inhibited: S26‑p, S136‑p
intracellular localization: S112‑p, S136‑p
molecular association, regulation: S112‑p, S128‑p, S136‑p, S155‑p, T201‑p
phosphorylation: S136‑p

Modification Sites and Domains  
Click here to view other types of protein modifications

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

 
1 0 S26‑p KSDPGIRsLGSDAGG
0 8 S67 TDRGLGPSLTEDQPG
0 2 G74 SLTEDQPGPyLAPGL
0 5 Y76‑p TEDQPGPyLAPGLLG
0 1 - gap
1 0 T94‑p HQQGRAAtNsHHGGA
1 2 S96‑p QGRAAtNsHHGGAGA
0 30 S108‑p AGAMETRsRHssyPA
2 78 S111‑p METRsRHssyPAGtE
116 185 S112‑p ETRsRHssyPAGtEE
0 51 Y113‑p TRsRHssyPAGtEED
1 31 T117‑p HssyPAGtEEDEGME
7 45 S128‑p EGMEEELsPFRGRsR
1 0 R131 EEELsPFRGRsRsAP
1 0 R133 ELsPFRGRsRsAPPN
1 23 S134‑p LsPFRGRsRsAPPNL
116 254 S136‑p PFRGRsRsAPPNLWA
0 1 Y147 NLWAAQRYGRELRRM
45 208 S155‑p GRELRRMsDEFEGsF
1 4 S161‑p MsDEFEGsFKGLPRP
6 16 S170‑p KGLPRPKsAGtATQM
2 0 T173‑p PRPKsAGtATQMRQS
0 1 T175 PKsAGtATQMRQSAG
0 1 S180 tATQMRQSAGWTRII
0 2 G182 TQMRQSAGWTRIIQS
0 5 K197 WWDRNLGKGGStPSQ
3 0 T201‑p NLGKGGStPSQ____
5193 : Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated)
5284 : Phospho-Bad (Ser112) (40A9) Rabbit mAb
9291 : Phospho-Bad (Ser112) Antibody
9296 : Phospho-Bad (Ser112) (7E11) Mouse mAb
11865 : Phospho-Bad (Ser112) (40A9) Rabbit mAb (PE Conjugate)
4366 : Phospho-Bad (Ser136) (D25H8) Rabbit mAb
5286 : Phospho-Bad (Ser136) (185D10) Rabbit mAb
9295 : Phospho-Bad (Ser136) Antibody
9297 : Phospho-Bad (Ser155) Antibody
  human

 
- gap
S25‑p AERGLGPsPAGDGPs
S32‑p sPAGDGPsGsGkHHR
S34‑p AGDGPsGsGkHHRQA
K36‑ac DGPsGsGkHHRQAPG
S57 HQQEQPTSSSHHGGA
S59 QEQPTSSSHHGGAGA
S71‑p AGAVEIRsRHssyPA
S74‑p VEIRsRHssyPAGtE
S75‑p EIRsRHssyPAGtED
Y76‑p IRsRHssyPAGtEDD
T80‑p HssyPAGtEDDEGMG
S91‑p EGMGEEPsPFrGrsR
R94‑m2 GEEPsPFrGrsRsAP
R96‑m2 EPsPFrGrsRsAPPN
S97‑p PsPFrGrsRsAPPNL
S99‑p PFrGrsRsAPPNLWA
Y110‑p NLWAAQRyGRELRRM
S118‑p GRELRRMsDEFVDsF
S124‑p MsDEFVDsFKKGLPR
S134‑p KGLPRPKsAGtAtQM
T137‑p PRPKsAGtAtQMRQS
T139‑p PKsAGtAtQMRQSSs
S144 tAtQMRQSSsWTRVF
S146‑p tQMRQSSsWTRVFQS
R161‑m1 WWDRNLGrGSSAPSQ
A165 NLGrGSSAPSQ____
5193 : Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated)
5284 : Phospho-Bad (Ser112) (40A9) Rabbit mAb
9291 : Phospho-Bad (Ser112) Antibody
9296 : Phospho-Bad (Ser112) (7E11) Mouse mAb
11865 : Phospho-Bad (Ser112) (40A9) Rabbit mAb (PE Conjugate)
4366 : Phospho-Bad (Ser136) (D25H8) Rabbit mAb
5286 : Phospho-Bad (Ser136) (185D10) Rabbit mAb
9295 : Phospho-Bad (Ser136) Antibody
9297 : Phospho-Bad (Ser155) Antibody
  rat

 
S26 KSDPGIRSLGSDAGG
S67 TDRGLGPSLTEDQPG
G74 SLTEDQPGPYLAPGL
Y76 TEDQPGPYLAPGLLG
- gap
N95 QQPGQAANNSHHGGA
S97 PGQAANNSHHGGAGT
S109 AGTMETRSRHSsYPA
S112 METRSRHSsYPAGtE
S113‑p ETRSRHSsYPAGtEE
Y114 TRSRHSsYPAGtEED
T118‑p HSsYPAGtEEDEGME
S129‑p EGMEEELsPFRGRsR
R132 EEELsPFRGRsRsAP
R134 ELsPFRGRsRsAPPN
S135‑p LsPFRGRsRsAPPNL
S137‑p PFRGRsRsAPPNLWA
Y148 NLWAAQRYGRELRRM
S156‑p GRELRRMsDEFEGSF
S162 MsDEFEGSFKGLPRP
S171 KGLPRPKSAGTATQM
T174 PRPKSAGTATQMRQs
T176 PKSAGTATQMRQsAs
S181‑p TATQMRQsAsWTRII
S183‑p TQMRQsAsWTRIIQS
K198 WWDRNLGKGGSTPSQ
T202 NLGKGGSTPSQ____
5193 : Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated)
5284 : Phospho-Bad (Ser112) (40A9) Rabbit mAb
9291 : Phospho-Bad (Ser112) Antibody
9296 : Phospho-Bad (Ser112) (7E11) Mouse mAb
11865 : Phospho-Bad (Ser112) (40A9) Rabbit mAb (PE Conjugate)
4366 : Phospho-Bad (Ser136) (D25H8) Rabbit mAb
5286 : Phospho-Bad (Ser136) (185D10) Rabbit mAb
9295 : Phospho-Bad (Ser136) Antibody
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