The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Heterodimer with UBE2V2. Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain); the complex has a specific 'Lys-63'-linked polyubiquitination activity. Interacts with RNF8 and RNF168. Interacts with RNF11. Interacts with the E3 ligases, HLTF and SHPRH; the interactions promote the 'Lys-63'-linked polyubiquitination of PCNA upon genotoxic stress and lead to DNA repair. Interacts with ARIH2 (via RING-type 2). Belongs to the ubiquitin-conjugating enzyme family. Note: This description may include information from UniProtKB.
Protein type: Ligase; Ubiquitin ligase; EC 126.96.36.199; DNA repair, damage; Ubiquitin conjugating system
Molecular Function: protein binding; ubiquitin binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity
Biological Process: activation of NF-kappaB transcription factor; DNA double-strand break processing; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; histone ubiquitination; positive regulation of DNA repair; positive regulation of histone modification; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of ubiquitin-protein ligase activity; postreplication repair; protein modification process; protein ubiquitination; proteolysis; regulation of DNA repair; regulation of histone ubiquitination; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway
LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.