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Protein Page:
AP-2 alpha (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
AP-2 alpha Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region. Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I. Interacts with RALBP1 in a complex also containing EPN1 and NUMB during interphase and mitosis. Interacts with KCTD1; this interaction represses transcription activation. Interacts (via C-terminus) with CITED2 (via C-terminus); the interaction stimulates TFAP2A- transcriptional activation. Interacts (via N-terminus) with EP300 (via N-terminus); the interaction requires CITED2. Belongs to the AP-2 family. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 6p24
Cellular Component: centrosome; cytoplasm; cytosol; Golgi apparatus; intracellular membrane-bound organelle; nucleoplasm; nucleus
Molecular Function: chromatin binding; protein binding; protein dimerization activity; protein homodimerization activity; sequence-specific DNA binding; transcription coactivator activity; transcription factor binding
Biological Process: embryonic cranial skeleton morphogenesis; embryonic forelimb morphogenesis; inner ear morphogenesis; kidney development; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; oculomotor nerve formation; palate development; positive regulation of bone mineralization; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of cell differentiation; response to lipopolysaccharide; response to water deprivation; Schwann cell development; sensory perception of sound; transcription from RNA polymerase II promoter; trigeminal nerve development
Disease: Branchiooculofacial Syndrome
Reference #:  P05549 (UniProtKB)
Alt. Names/Synonyms: Activating enhancer-binding protein 2-alpha; Activator protein 2; AP-2; AP-2 transcription factor; AP-2alpha; AP2-alpha; AP2A; AP2TF; BOFS; FLJ51761; TFAP2; TFAP2A; transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha); Transcription factor AP-2-alpha
Gene Symbols: TFAP2A
Molecular weight: 48,062 Da
Basal Isoelectric point: 8.1  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

AP-2 alpha

Protein Structure Not Found.


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Sites Implicated In
transcription, altered: S258‑p
transcription, induced: S219‑p, S429‑p
molecular association, regulation: S219‑p, S258‑p
protein stabilization: S429‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 Y73‑p YPQSQDPySHVNDPY
0 1 S119‑p RGLPHQLsGLDPRRD
0 1 S181‑p VIKKGPVsLSKsNsN
0 1 S185‑p GPVsLSKsNsNAVSA
0 4 S187‑p VsLSKsNsNAVSAIP
1 0 S219‑p CSVPGRLsLLSSTSK
1 5 S239‑p AEVQRRLsPPECLNA
2 0 S258‑p GVLRRAKsKNGGRSL
0 1 R263 AKsKNGGRSLREKLD
1 0 S326‑p EFLNRQHsDPNEQVT
0 1 S428‑p HTDNNAKssDKEEKH
1 0 S429‑p TDNNAKssDKEEKHR
  AP-2 alpha iso2  
Y67 YPQSQDPYSHVNDPY
S113 RGLPHQLSGLDPRRD
S175 VIKKGPVSLSKSNSN
S179 GPVSLSKSNSNAVSA
S181 VSLSKSNSNAVSAIP
S213 CSVPGRLSLLSSTSK
S233 AEVQRRLSPPECLNA
S252 GVLRRAKSKNGGRSL
R257 AKSKNGGRSLREKLD
S320 EFLNRQHSDPNEQVT
S422 HTDNNAKSSDKEEKH
S423 TDNNAKSSDKEEKHR
  mouse

 
Y73 YPQSQDPYSHVNDPY
S119‑p RGLPHQLsGLDPRRD
S181 VIKKGPVSLSKSNsN
S185 GPVSLSKSNsNAVSA
S187‑p VSLSKSNsNAVSAIP
S219 CSVPGRLSLLSSTSK
S239‑p AEVQRRLsPPECLNA
S258‑p GVLRRAKsKNGGrSL
R263‑m1 AKsKNGGrSLREKLD
S326 EFLNRQHSDPNEQVA
S428 HTDNSAKSSDKEEKH
S429 TDNSAKSSDKEEKHR
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