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Protein Page:
PSMB5 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PSMB5 The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage through the Nrf2-ARE pathway. Belongs to the peptidase T1B family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Proteasome complex; EC 3.4.25.1; Protease
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: centrosome; cytosol; nucleoplasm; nucleus; proteasome complex; proteasome core complex
Molecular Function: peptidase activity; protein binding
Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; proteolysis; regulation of amino acid metabolic process; regulation of mRNA stability; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway
Reference #:  P28074 (UniProtKB)
Alt. Names/Synonyms: LMPX; Macropain epsilon chain; MB1; MGC104214; Multicatalytic endopeptidase complex epsilon chain; proteasome (prosome, macropain) subunit, beta type, 5; proteasome beta 5 subunit; proteasome catalytic subunit 3; Proteasome chain 6; Proteasome epsilon chain; Proteasome subunit beta type-5; Proteasome subunit MB1; Proteasome subunit X; proteasome subunit, beta type, 5; PSB5; PSMB5; PSX large multifunctional protease X; X
Gene Symbols: PSMB5
Molecular weight: 28,480 Da
Basal Isoelectric point: 6.44  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PSMB5

Protein Structure Not Found.
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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S87‑p TAGAYIAsQTVkkVI
0 55 K91‑ub YIAsQTVkkVIEINP
0 7 K92‑ub IAsQTVkkVIEINPy
0 5 Y99‑p kVIEINPyLLGTMAG
0 2 S139‑p RISVAAAsKLLANMV
0 1 Y147‑p KLLANMVyQyKGMGL
0 40 Y149‑p LANMVyQyKGMGLSM
0 1 T158 GMGLSMGTMICGWDK
0 15 Y171‑p DKRGPGLyYVDsEGN
0 1 S175‑p PGLyYVDsEGNRISG
0 1 E207 RGYSYDLEVEQAyDL
0 3 E207 RGYSYDLEVEQAyDL
0 1 Y212‑p DLEVEQAyDLARRAI
0 125 Y220‑p DLARRAIyQATYRDA
0 34 Y228‑p QATYRDAySGGAVNL
0 64 Y236‑p SGGAVNLyHVREDGW
0 2 S248‑p DGWIRVSsDNVADLH
0 1 K257‑ac NVADLHEkYSGstP_
0 4 K257‑ub NVADLHEkYSGstP_
0 2 S261‑p LHEkYSGstP_____
0 19 T262‑p HEkYSGstP______
  PSMB5 iso2  
S87 TAGAYIASQTVKKVI
K91 YIASQTVKKVIEINP
K92 IASQTVKKVIEINPY
Y99 KVIEINPYLLGTMAG
S139 RISVAAASKLLANMV
Y147 KLLANMVYQYKGMGL
Y149 LANMVYQYKGMGLSM
T158‑p GMGLSMGtMICGWDK
Y183 KPKSFGMYLFCGCAE
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  PSMB5 iso3  
- gap
- gap
- gap
- gap
S36 RISVAAASKLLANMV
Y44 KLLANMVYQYKGMGL
Y46 LANMVYQYKGMGLSM
T55 GMGLSMGTMICGWDK
Y68 DKRGPGLYYVDSEGN
S72 PGLYYVDSEGNRISG
E104 RGYSYDLEVEQAYDL
E104 RGYSYDLEVEQAYDL
Y109 DLEVEQAYDLARRAI
Y117 DLARRAIYQATYRDA
Y125 QATYRDAYSGGAVNL
Y133 SGGAVNLYHVREDGW
S145 DGWIRVSSDNVADLH
K154 NVADLHEKYSGSTP_
K154 NVADLHEKYSGSTP_
S158 LHEKYSGSTP_____
T159 HEKYSGSTP______
  mouse

 
S87 TAGAYIASQTVkkVI
K91‑ub YIASQTVkkVIEINP
K92‑ub IASQTVkkVIEINPY
Y99 kVIEINPYLLGTMAG
S139 RISVAAASKLLANMV
Y147 KLLANMVYQYKGMGL
Y149 LANMVYQYKGMGLSM
T158 GMGLSMGTMICGWDK
Y171‑p DKRGPGLyYVDSEGN
S175 PGLyYVDSEGNRISG
K207‑ac RGYSYDLkVEEAYDL
K207‑ub RGYSYDLkVEEAYDL
Y212 DLkVEEAYDLARRAI
Y220‑p DLARRAIyQATYRDA
Y228 QATYRDAYSGGAVNL
Y236‑p SGGAVNLyHVREDGW
S248 DGWIRVSSDNVADLH
K257‑ac NVADLHDkYSSVSVP
K257 NVADLHDKYSSVSVP
S262 HDkYSSVSVP_____
V263 DkYSSVSVP______
  rat

 
S87 TAGAYIASQTVkKVI
K91‑ub YIASQTVkKVIEINP
K92 IASQTVkKVIEINPY
Y99 KVIEINPYLLGTMAG
S139 RISVAAASKLLANMV
Y147 KLLANMVYQyKGMGL
Y149‑p LANMVYQyKGMGLSM
T158 GMGLSMGTMICGWDK
Y171 DKRGPGLYYVDSEGN
S175 PGLYYVDSEGNRISG
Q207 RGYSYDLQVEEAYDL
Q207 RGYSYDLQVEEAYDL
Y212 DLQVEEAYDLARRAI
Y220 DLARRAIYQATYRDA
Y228 QATYRDAYSGGAVNL
Y236 SGGAVNLYHVREDGW
S248 DGWIRVSSDNVADLH
K257 NVADLHDKYTSSIP_
K257 NVADLHDKYTSSIP_
S261 LHDKYTSSIP_____
I262 HDKYTSSIP______
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