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Protein Page:
AMBRA1 (human)
rdtyret
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
AMBRA1 a WD40 protein that regulates autophagy and development of the nervous system. A highly conserved vertebrate protein whose expression is mostly confined to the neuroepithelium during early neurogenesis. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. Interacts with ATG6. Forms a complex with ATG6 and PIK3C3. Its functional inactivation in mouse is lethal in utero (starting from embryonic day 14.5), leading to severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, and excessive apoptosis. Hyperproliferation followed by a wave of caspase-dependent cell death was the earliest detectable abnormality in the developing neuroepithelium. Five isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Autophagy; Vesicle
Chromosomal Location of Human Ortholog: 11p11.2
Cellular Component: autophagic vacuole; axoneme; cytoplasm; cytosol; mitochondrial outer membrane; mitochondrion; perinuclear region of cytoplasm; phagocytic vesicle
Molecular Function: protein binding; ubiquitin protein ligase binding
Biological Process: cell differentiation; macroautophagy; negative regulation of cell proliferation; negative regulation of neuron apoptosis; neural tube development; positive regulation of phosphoinositide 3-kinase activity
Reference #:  Q9C0C7 (UniProtKB)
Alt. Names/Synonyms: activating molecule in beclin-1-regulated autophagy; Activating molecule in BECN1-regulated autophagy protein 1; AMBRA1; AMRA1; autophagy/beclin-1 regulator 1; DCAF3; DDB1 and CUL4 associated factor 3; FLJ20294; KIAA1736; MGC33725; WD repeat domain 94; WDR94
Gene Symbols: AMBRA1
Molecular weight: 142,507 Da
Basal Isoelectric point: 6.73  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

AMBRA1

Protein Structure Not Found.


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Modification Sites and Domains  
Click here to view other types of protein modifications

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
3 2 S52‑p KRVELPDsPRSTFLL
0 11 K175‑ub REPFAVVkTASEMER
0 1 R226‑m2 ELSHYRQrALLQSQP
0 1 S328‑p SLLPHQDsVPPASAR
0 1 S369 QGLLNRPSAFSTVQS
0 1 S372 LNRPSAFSTVQSSTA
0 1 T373 NRPSAFSTVQSSTAG
0 2 S387‑p GNTLRNLsLGPtRRs
0 2 T391‑p RNLsLGPtRRsLGGP
0 2 S394‑p sLGPtRRsLGGPLSS
0 2 S443‑p PPPRTSAsSVSLLSV
1 0 S465‑p SQASVYTsATEGRGF
0 1 S628‑p RTEGQTPssSRLELs
0 1 S629‑p TEGQTPssSRLELsS
1 2 S635‑p ssSRLELsSSAsPQE
0 13 S639‑p LELsSSAsPQEERTV
0 10 S696‑p LRRRLLEsSLISLSR
0 1 Y704‑p SLISLSRyDGAGSRE
0 1 Y715‑p GSREHPIyPDPARLs
0 2 S722‑p yPDPARLsPAAyYAQ
0 1 Y726‑p ARLsPAAyYAQRMIQ
0 1 R747‑m2 SIRQRSMrYQQNRLR
0 2 S797‑p APRNARMsAPsLGRF
0 1 S800‑p NARMsAPsLGRFVPR
0 4 Y814‑p RRFLLPEyLPYAGIF
0 1 Y1026‑p EPGLGLAyGtNKGDL
0 1 T1028‑p GLGLAyGtNKGDLVI
0 3 S1192‑p SHRIHRSsQTGTEPG
0 1 T1203‑p TEPGAAHtssPQPST
0 3 S1204‑p EPGAAHtssPQPSTS
0 3 S1205‑p PGAAHtssPQPSTSR
0 1 T1238‑p ASWDQPGtPGREPTQ
  AMBRA1 iso2  
S52 KRVELPDSPRSTFLL
K175 REPFAVVKTASEMER
R226 ELSHYRQRALLQSQP
S328 SLLPHQDSVPPASAR
S369 QGLLNRPSAFSTVQS
S372 LNRPSAFSTVQSSTA
T373 NRPSAFSTVQSSTAG
S387 GNTLRNLSLGPTRRS
T391 RNLSLGPTRRSLGGP
S394 SLGPTRRSLGGPLSS
S443 PPPRTSASSVSLLSV
S465 SQASVYTSATEGRGF
S628 RTEGQTPSSSRLELS
S629 TEGQTPSSSRLELSS
S635 SSSRLELSSSASPQE
S639 LELSSSASPQEERTV
S696 LRRRLLESSLISLSR
Y704 SLISLSRYDGAGSRE
Y715 GSREHPIYPDPARDN
- gap
- gap
- gap
S737 APRNARMSAPSLGRF
S740 NARMSAPSLGRFVPR
Y754 RRFLLPEYLPYAGIF
Y966 EPGLGLAYGTNKGDL
T968 GLGLAYGTNKGDLVI
S1132 SHRIHRSSQTGTEPG
T1143 TEPGAAHTSSPQPST
S1144 EPGAAHTSSPQPSTS
S1145 PGAAHTSSPQPSTSR
T1178 ASWDQPGTPGREPTQ
  mouse

 
S52‑p KRVELPDsPRSTFLL
K175‑ub REPFAVVkTASEMER
R226 ELSHYRQRALLQSQP
S329 SLLPHQDSVPPASAR
S370 QGLLNRPSAFSTVQS
S373 LNRPSAFSTVQSSTA
T374 NRPSAFSTVQSSTAG
S388 GNTLRNLSLGPTRRS
T392 RNLSLGPTRRSLGGP
S395 SLGPTRRSLGGPLSS
S444 PPPRTSASSVSLLSV
S466 SQASVYTSATEGRGF
S629 RTEGQMPSSSRLELS
S630 TEGQMPSSSRLELSS
S636 SSSRLELSSSASSQE
S640 LELSSSASSQEERTV
S697 LRRRLLESSLISLSR
Y705 SLISLSRYDGAGSRE
Y716 GSREHPIYPDPARLS
S723 YPDPARLSPAAYYAQ
Y727 ARLSPAAYYAQRMIQ
R748‑m2 SIRQRSMrYQQNRLR
S798 APRNARMSAPSLGRF
S801 NARMSAPSLGRFVPR
Y815 RRFLLPEYLPYAGIF
Y1027 EPGLGLAYGTNKGDL
T1029 GLGLAYGTNKGDLVI
S1193 SHRIHRSSQTGTESG
T1204 TESGAARTSsPQPST
S1205 ESGAARTSsPQPSTS
S1206‑p SGAARTSsPQPSTSR
T1239 ASWDQPSTSGRELPQ
  rat

 
S52 KRVELPDSPRSTFLL
K175 REPFAVVKTASEMER
R226 ELSHYRQRALLQSQP
- gap
S279‑p QGLLNRPsAFstVQS
S282‑p LNRPsAFstVQSSTA
T283‑p NRPsAFstVQSSTAG
S297‑p GNTLRNLsLGPtRRS
T301‑p RNLsLGPtRRSLGGP
S304 sLGPtRRSLGGPLSS
S353 PPPRTSASSVSLLSV
S375 SQASVYTSATEGRGF
N538 RTEGQMPNSSRLELS
S539 TEGQMPNSSRLELSS
S545 NSSRLELSSSASSQE
S549 LELSSSASSQEERTV
S606 LRRRLLESSLISLSR
Y614 SLISLSRYDGAGSRE
Y625 GSREHPIYPDPARLS
S632 YPDPARLSPAAYYAQ
Y636 ARLSPAAYYAQRMIQ
R657 SIRQRSMRYQQNRLR
S707 APRNARMSAPSLGRF
S710 NARMSAPSLGRFVPR
Y724 RXELLPAYMPVRGFL
Y939 EPGLGLAYGTNKGDL
T941 GLGLAYGTNKGDLVI
S1105 SHRIHRSSQTGTESG
T1116 TESGAARTSSPQPST
S1117 ESGAARTSSPQPSTS
S1118 SGAARTSSPQPSTSR
T1151 ASWDQPSTSGRELPQ
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