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Protein Page:
TBK1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
TBK1 a protein kinase of the IKK family. Can mediate NFkB activation in response to certain growth factors. Forms a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.1; Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; IKK family
Chromosomal Location of Human Ortholog: 12q14.1
Cellular Component: cytoplasm; cytosol; endosome membrane
Molecular Function: phosphoprotein binding; protein binding; protein kinase activity; protein serine/threonine kinase activity
Biological Process: I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; interferon type I production; negative regulation of interferon type I production; peptidyl-serine phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of interferon-alpha production; positive regulation of interferon-beta production; positive regulation of transcription from RNA polymerase II promoter; response to virus
Disease: Frontotemporal Dementia And/or Amyotrophic Lateral Sclerosis 4
Reference #:  Q9UHD2 (UniProtKB)
Alt. Names/Synonyms: FLJ11330; NAK; NF-kappa-B-activating kinase; NF-kB-activating kinase; Serine/threonine-protein kinase TBK1; T2K; TANK-binding kinase 1; TBK1
Gene Symbols: TBK1
Molecular weight: 83,642 Da
Basal Isoelectric point: 6.32  Predict pI for various phosphorylation states
CST Pathways:  NF-kB Signaling  |  Toll-Like Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

TBK1

Protein Structure Not Found.
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Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
transcription, induced: K30‑ub, S172‑p, K401‑ub
transcription, inhibited: S527‑p
activity, inhibited: S172‑p
enzymatic activity, induced: K30‑ub, S172‑p, K401‑ub
protein degradation: S527‑p
ubiquitination: K30‑ub, K401‑ub, S527‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 0 K30 VFRGRHKKTGDLFAI
1 0 K30‑ub VFRGRHKkTGDLFAI
0 1 K38 TGDLFAIKVFNNISF
1 0 K69‑ub LNHKNIVkLFAIEEE
0 1 S151‑p VIGEDGQsVykLTDF
0 3 Y153‑p GEDGQsVykLTDFGA
1 0 K154 EDGQsVyKLTDFGAA
1 3 K154‑ub EDGQsVykLTDFGAA
12 49 S172‑p EDDEQFVsLyGTEEY
0 3 Y174‑p DEQFVsLyGTEEYLH
1 0 K236 RNKEVMYKIITGkPS
1 0 K241 MYKIITGKPSGAIsG
0 1 K241‑ub MYKIITGkPSGAIsG
0 1 S247‑p GkPSGAIsGVQKAEN
1 0 K251 GAIsGVQKAENGPID
0 1 T278‑p RGLQVLLtPVLANIL
0 1 K291‑ub ILEADQEkCWGFDQF
0 1 K344‑ub ELVYKQTkIISSNQE
0 2 Y354‑p SSNQELIyEGRRLVL
1 0 K372‑ub RLAQHFPkTTEENPI
0 1 K396‑ub TIGLIYEkISLPkVH
1 0 K401‑ub YEkISLPkVHPRYDL
0 1 S499 AAELGEISDIHtkLL
0 1 T503‑p GEISDIHtkLLRLSS
0 1 K504‑ub EISDIHtkLLRLSSs
0 1 S511‑p kLLRLSSsQGTIETS
1 0 S527‑p QDIDSRLsPGGSLAD
0 1 K584‑ub YNEEQIHkFDKQKLY
1 0 K646 DIEEEVSKYQEYTNE
0 1 K661‑ub LQETLPQkMFtASSG
0 1 T664‑p TLPQkMFtASSGIKH
1 0 K691 VEMTLGMKKLKEEME
1 17 S716‑p HILERFGsLTMDGGL
5483 : Phospho-TBK1/NAK (Ser172) (D52C2) XP(R) Rabbit mAb
13498 : Phospho-TBK1/NAK (Ser172) (D52C2) XP(R) Rabbit mAb (PE Conjugate)
  mouse

 
K30‑ac VFRGRHKkTGDLYAV
K30 VFRGRHKKTGDLYAV
K38‑ub TGDLYAVkVFNNISF
K69 LNHKNIVKLFAIEEE
S151 VIGEDGQSVYkLTDF
Y153 GEDGQSVYkLTDFGA
K154‑ac EDGQSVYkLTDFGAA
K154 EDGQSVYKLTDFGAA
S172‑p EDDEQFVsLYGTEEY
Y174 DEQFVsLYGTEEYLH
K236‑ac RNKEVMYkIITGkPS
K241‑ac MYkIITGkPSGAISG
K241 MYkIITGKPSGAISG
S247 GkPSGAISGVQkAEN
K251‑ac GAISGVQkAENGPID
T278 QGLQALLTPVLANIL
K291 ILEADQEKCWGFDQF
K344 ELVYKQTKIVSSNQE
Y354 SSNQELIYEGRRLVL
K372 RLAQHFPKTTEENPI
K396 TVGLRYEKISLPKIH
K401 YEKISLPKIHPRYDL
S499‑p AAELGEIsDIHTKLL
T503 GEIsDIHTKLLRLSS
K504 EIsDIHTKLLRLSSS
S511 KLLRLSSSQGTIESS
S527 QDISSRLSPGGLLAD
K584 YNEEQIHKFDKQKLY
K646‑ac DIEEEVSkYQDYTNE
K661 LQETLPQKMLAASGG
A664 TLPQKMLAASGGVKH
K691‑ac VEMTLGMkKLKEEME
S716‑p HILERFGsLTMDGGL
5483 : Phospho-TBK1/NAK (Ser172) (D52C2) XP(R) Rabbit mAb
13498 : Phospho-TBK1/NAK (Ser172) (D52C2) XP(R) Rabbit mAb (PE Conjugate)
  rat

 
K30 VFRGRHKKTGDLYAV
K30 VFRGRHKKTGDLYAV
K38 TGDLYAVKVFNNISF
K69 LNHKNIVKLFAIEEE
S151 VIGEDGQSVyKLTDF
Y153‑p GEDGQSVyKLTDFGA
K154 EDGQSVyKLTDFGAA
K154 EDGQSVyKLTDFGAA
S172 EDDEQFVSLYGTEEY
Y174 DEQFVSLYGTEEYLH
K236 RNKEVMYKIITGKPS
K241 MYKIITGKPSGAISG
K241 MYKIITGKPSGAISG
S247 GKPSGAISGVQKAEN
K251 GAISGVQKAENGPID
T278 QGLQALLTPVLANIL
K291 ILEADQEKCWGFDQF
K344 ELVYKQTKIISSNQE
Y354 SSNQELIYEGRRLVL
K372 RLAQHFPKTTEENPI
K396 SIGLRYEKISLPKIH
K401 YEKISLPKIHPRYDL
S499 AAELGEISDIHTKLL
T503 GEISDIHTKLLRLSS
K504 EISDIHTKLLRLSSS
S511 KLLRLSSSQGTIESS
S527 QDINSRLSPGGSLAD
K584 YNEEQIHKFDKQKLY
K646 DIEEEVSKYQDYTNE
K661 LQETLPQKMLAASSG
A664 TLPQKMLAASSGIKH
K691 VEMTLGMKKLKEEME
S716 HILERFGSLTMDGGL
5483 : Phospho-TBK1/NAK (Ser172) (D52C2) XP(R) Rabbit mAb
13498 : Phospho-TBK1/NAK (Ser172) (D52C2) XP(R) Rabbit mAb (PE Conjugate)
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