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Protein Page:
MST1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
MST1 a protein kinase of the STE20 family. Proteolytically activated by caspase during apoptosis. Activated by apoptotic signals as well as other stress conditions. Full activation requires both phosphorylation and caspase-mediated cleavage. Phosphorylation at serine 327 of Mst1, which is close to the caspase-3 recognition site, inhibits caspase-mediated cleavage. Note: This description may include information from UniProtKB.
Protein type: Autophagy; Protein kinase, STE; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; STE group; STE20 family; MST subfamily
Chromosomal Location of Human Ortholog: 20q11.2-q13.2
Cellular Component: cytoplasm; cytosol; nucleus; protein complex
Molecular Function: ATP binding; identical protein binding; magnesium ion binding; protein binding; protein dimerization activity; protein homodimerization activity; protein kinase activity; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity; transcription factor binding
Biological Process: apoptosis; cell morphogenesis; peptidyl-serine phosphorylation; positive regulation of apoptosis; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein binding; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein stabilization; signal transduction
Disease: T-cell Immunodeficiency, Recurrent Infections, And Autoimmunity With Or Without Cardiac Malformations
Reference #:  Q13043 (UniProtKB)
Alt. Names/Synonyms: dJ211D12.2 (serine/threonine kinase 4 (MST1, KRS2)); DKFZp686A2068; kinase responsive to stress 2; KRS2; Mammalian STE20-like protein kinase 1; mammalian sterile 20-like 1; MST-1; MST1; serine/threonine kinase 4; Serine/threonine-protein kinase 4; Serine/threonine-protein kinase Krs-2; STE20-like kinase MST1; STK4; yeast Ste20-like; YSK3
Gene Symbols: STK4
Molecular weight: 55,630 Da
Basal Isoelectric point: 4.97  Predict pI for various phosphorylation states
CST Pathways:  Hippo Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

MST1

Protein Structure Not Found.
Download PyMol Script
Download ChimeraX Script

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
apoptosis, altered: S82‑p, T120‑p, T387‑p
cell growth, induced: T120‑p
transcription, induced: T120‑p
enzymatic activity, induced: S82‑p, T183‑p, T387‑p, Y433‑p
enzymatic activity, inhibited: T120‑p
intracellular localization: S82‑p, T120‑p, T183‑p
molecular association, regulation: S82‑p
phosphorylation: T120‑p
protein stabilization: Y433‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 T3‑p _____MEtVQLRNPP
1 1 K35‑ac EVFDVLEkLGEGsyG
0 25 S40‑p LEkLGEGsyGsVyKA
0 16 Y41‑p EkLGEGsyGsVyKAI
0 5 S43‑p LGEGsyGsVyKAIHK
0 3 Y45‑p EGsyGsVyKAIHKET
1 0 S82‑p SIMQQCDsPHVVKYY
0 2 K119‑ub DIIRLRNktLTEDEI
3 0 T120‑p IIRLRNktLTEDEIA
0 1 S132‑p EIATILQsTLKGLEY
0 1 K164‑ub LNTEGHAkLADFGVA
1 8 T177‑p VAGQLTDtMAkRNtV
0 3 K180‑ub QLTDtMAkRNtVIGT
13 6 T183‑p DtMAkRNtVIGTPFW
0 1 K246‑ub NPPPTFRkPELWSDN
0 1 S265‑p VKQCLVKsPEQRATA
0 1 S288‑p VRSAKGVsILRDLIN
0 11 S307‑p VKLKRQEsQQREVDQ
0 62 S320‑p DQDDEENsEEDEMDs
2 1 S327‑p sEEDEMDsGtMVRAV
0 6 T329‑p EDEMDsGtMVRAVGD
1 27 T340‑p AVGDEMGtVRVASTM
0 1 T346 GtVRVASTMTDGANT
0 1 T360‑p TMIEHDDtLPSQLGt
0 1 T367‑p tLPSQLGtMVINAED
0 6 T380‑p EDEEEEGtMKRRDEt
3 9 T387‑p tMKRRDEtMQPAKPs
0 1 S394‑p tMQPAKPsFLEyFEQ
0 17 Y398‑p AKPsFLEyFEQKEKE
0 9 S410‑p EKENQINsFGKsVPG
0 4 S414‑p QINsFGKsVPGPLKN
0 7 P418 FGKsVPGPLKNssDW
0 2 S422‑p VPGPLKNssDWKIPQ
0 1 S423‑p PGPLKNssDWKIPQD
2 120 Y433‑p KIPQDGDyEFLKsWt
1 8 S438‑p GDyEFLKsWtVEDLQ
0 1 T440‑p yEFLKsWtVEDLQKR
3681 : Phospho-Mst1 (Thr183)/Mst2 (Thr180) Antibody
  MST1 iso2  
T3 _____METVQLRNPP
K35 EVFDVLEKLGEGSYG
S40 LEKLGEGSYGSVYKA
Y41 EKLGEGSYGSVYKAI
S43 LGEGSYGSVYKAIHK
Y45 EGSYGSVYKAIHKET
S82 SIMQQCDSPHVVKYY
K119 DIIRLRNKTLTEDEI
T120 IIRLRNKTLTEDEIA
S132 EIATILQSTLKGLEY
K164 LNTEGHAKLADFGVA
T177 VAGQLTDTMAKRNTV
K180 QLTDTMAKRNTVIGT
T183 DTMAKRNTVIGTPFW
K246 NPPPTFRKPELWSDN
S265 VKQCLVKSPEQRATA
S288 VRSAKGVSILRDLIN
S307 VKLKRQESQQREVDQ
S320 DQDDEENSEEDEMDS
S327 SEEDEMDSGTMVRAV
T329 EDEMDSGTMVRAVGD
T340 AVGDEMGTVRVASTM
T346 GTVRVASTMTDGANT
T360 TMIEHDDTLPSQLGT
T367 TLPSQLGTMVINAED
T380 EDEEEEGTMKRRDET
T387 TMKRRDETMQPAKPS
S394 TMQPAKPSFLEYFEQ
Y398 AKPSFLEYFEQKEKE
S410 EKENQINSFGKSVPG
S414 QINSFGKSVPGPLKN
P418 FGKSVPGPLKNSSDW
S422 VPGPLKNSSDWKIPQ
S423 PGPLKNSSDWKIPQD
Y433 KIPQDGDYEFKTSQE
S438 GDYEFKTSQEQQSGK
E440 YEFKTSQEQQSGKDI
  mouse

 
T3 _____METVQLRNPP
K35 EVFDVLEKLGEGSYG
S40 LEKLGEGSYGSVYKA
Y41 EKLGEGSYGSVYKAI
S43 LGEGSYGSVYKAIHK
Y45 EGSYGSVYKAIHKET
S82 SIMQQCDSPHVVKYY
K119 DIIRLRNKTLTEDEI
T120 IIRLRNKTLTEDEIA
S132 EIATILQSTLKGLEY
K164 LNTEGHAKLADFGVA
T177‑p VAGQLTDtMAKRNtV
K180 QLTDtMAKRNtVIGT
T183‑p DtMAKRNtVIGTPFW
K246 NPPPTFRKPELWSDN
S265 VKQCLVKSPEQRATA
S288‑p VKSAKGVsILRDLIN
A307 VKLKRQEAQQREVDQ
S320‑p DQDDEENsEEDEMDs
S327‑p sEEDEMDsGtMVRAA
T329‑p EDEMDsGtMVRAAGD
T340‑p AAGDEMGtVRVAStM
T346‑p GtVRVAStMSGGANT
T360 TMIEHGDTLPSQLGT
T367 TLPSQLGTMVINTED
T380‑p EDEEEEGtMKRRDEt
T387‑p tMKRRDEtMQPAKPS
S394 tMQPAKPSFLEYFEQ
Y398 AKPSFLEYFEQKEKE
S410‑p EKENQINsFGKNVSG
N414 QINsFGKNVSGsLKN
S418‑p FGKNVSGsLKNSSDW
S422 VSGsLKNSSDWKIPQ
S423 SGsLKNSSDWKIPQD
Y433‑p KIPQDGDyEFLKsWT
S438‑p GDyEFLKsWTVEDLQ
T440 yEFLKsWTVEDLQKR
3681 : Phospho-Mst1 (Thr183)/Mst2 (Thr180) Antibody
  rat

 
T3 _____METVQLRNPP
K35 EVFDVLEKLGEGsYG
S40‑p LEKLGEGsYGSVYKA
Y41 EKLGEGsYGSVYKAI
S43 LGEGsYGSVYKAIHK
Y45 EGsYGSVYKAIHKET
S82 SIMQQCDSPHVVKYY
K119 DIIRLRNKTLTEDEI
T120 IIRLRNKTLTEDEIA
S132 EIATILQSTLKGLEY
K164 LNTEGHAKLADFGVA
T177 VAGQLTDTMAKRNTV
K180 QLTDTMAKRNTVIGT
T183 DTMAKRNTVIGTPFW
K246 NPPPTFRKPEVWSDN
S265 VKQCLVKSPEQRATA
A288 VKSAKGAAILRDLIN
A307 VKLKRQEAQQRAVDQ
S320‑p DQDDDENsEEDEMDS
S327 sEEDEMDSGTMVRAA
T329 EDEMDSGTMVRAAGD
T340 AAGDDMGTVRVASTM
T346 GTVRVASTMSGGANT
T360 TMIEHGDTLPSQLGT
T367 TLPSQLGTMVINTED
T380 EDEEEEGTMKRRDET
T387 TMKRRDETMQPAKPS
S394 TMQPAKPSFLEYFEQ
Y398 AKPSFLEYFEQKEKE
S410‑p EKENQINsFGKNVSG
N414 QINsFGKNVSGsLKN
S418‑p FGKNVSGsLKNSSDW
S422 VSGsLKNSSDWKIPQ
S423 SGsLKNSSDWKIPQD
Y433 KIPQDGDYEFLKSWT
S438 GDYEFLKSWTVEDLQ
T440 YEFLKSWTVEDLQKR
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