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Protein Page:
ATG4A (human)

Overview
ATG4A Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family. 4 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Microtubule-binding; EC 3.4.22.-; Protease; Autophagy
Chromosomal Location of Human Ortholog: Xq22.3
Cellular Component: cytoplasm; cytosol
Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; protein binding
Biological Process: autophagic vacuole formation; C-terminal protein lipidation; mitochondrion degradation; protein delipidation; protein processing; protein targeting to membrane; proteolysis
Reference #:  Q8WYN0 (UniProtKB)
Alt. Names/Synonyms: APG4 autophagy 4 homolog A; APG4A; ATG4 autophagy related 4 homolog A (S. cerevisiae); ATG4A; AUT-like 2 cysteine endopeptidase; AUT-like 2, cysteine endopeptidase; AUTL2; autophagin 2; Autophagin-2; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; Cysteine protease ATG4A; hAPG4A
Gene Symbols: ATG4A
Molecular weight: 45,378 Da
Basal Isoelectric point: 4.93  Predict pI for various phosphorylation states
CST Pathways:  Autophagy Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ATG4A

Protein Structure Not Found.
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Modification Sites and Domains  

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S47‑p TEKSKLLsDISARLW
0 1 S100‑p RHLGRDWsWEKQKEQ
0 1 Y111‑p QKEQPKEyQRILQCF
  mouse

 
S47 TEKSKLLSDISARLW
N100 RHLGRDWNWERQKEQ
Y111 QKEQPKEYQRILQCF
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