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Protein Page:
Cot (human)

Overview
Cot an oncogenic Ser/Thr kinase of the STE group. Activates IkappaB kinases, thus inducing the nuclear translocation of NF-kappaB. Promotes the production of TNF-alpha and IL-2 during T lymphocyte activation. Interacts with NFkB-p105. Overexpressed and amplified in breast tumors. Viral insertions induce rat lymphomas and mouse mammary carcinomas. Isolated as a transforming factor in two cell lines. Mediates LPS activation of macrophages. 2 isoforms of the human protein are produced by alternative initiation. Note: This description may include information from UniProtKB.
Protein type: Oncoprotein; Protein kinase, STE; Kinase, protein; EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); STE group; STE-Unique family
Chromosomal Location of Human Ortholog: 10p11.23
Cellular Component: cytoplasm; cytosol
Molecular Function: MAP kinase kinase kinase activity; protein binding; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: apoptosis; protein amino acid phosphorylation; stress-activated MAPK cascade; T cell costimulation
Disease: Lung Cancer
Reference #:  P41279 (UniProtKB)
Alt. Names/Synonyms: c-COT; Cancer Osaka thyroid oncogene; COT; cot (cancer Osaka thyroid) oncogene; EST; ESTF; Ewing sarcoma transformant; FLJ10486; M3K8; MAP3K8; MEKK8; Mitogen-activated protein kinase kinase kinase 8; Proto-oncogene c-Cot; proto-oncogene serine/threoine protein kinase; Serine/threonine-protein kinase cot; TPL-2; TPL2; Tumor progression locus 2; tumor progression locus-2
Gene Symbols: MAP3K8
Molecular weight: 52,925 Da
Basal Isoelectric point: 5.54  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  B Cell Receptor Signaling  |  Growth And Differentiation Control by MAPKs  |  NF-kB Signaling  |  PI3K/Akt Signaling  |  SAPK/JNK Signaling Cascades
Select Structure to View Below

Cot

Protein Structure Not Found.
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Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
transcription, altered: S400‑p
enzymatic activity, induced: S62‑p, T290‑p
molecular association, regulation: T290‑p
phosphorylation: T290‑p
protein degradation: T290‑p

Modification Sites and Domains  

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
2 1 S62‑p QNDERSKsLLLSGQE
0 1 T80 LSSVRYGTVEDLLAF
0 1 S125‑p NGRYQIDsDVLLIPW
0 1 Y136‑p LIPWKLTyRNIGsDF
0 1 N138 PWKLTyRNIGsDFIP
0 26 S141‑p LTyRNIGsDFIPRGA
0 1 Y153‑p RGAFGKVyLAQDIKT
5 0 T290‑p FPKDLRGtEIYMSPE
0 1 S334‑p WVKRYPRsAYPSYLY
0 1 S368‑p MRELIEAsLERNPNH
3 1 S400‑p EDQPRCQsLDSALLE
1 0 S413 LERKRLLSRKELELP
1 1 S443 EMLKRQRSLYIDLGA
4491 : Phospho-Tpl2 (Ser400) Antibody
  mouse

 
S62‑p QNEERSEsLLRSGQE
T80 LSSVRYGTVEDLLAF
S125 NGRYQIDSDVLLVPW
Y136 LVPWKLTYRNIGsGF
N138 PWKLTYRNIGsGFVP
S141‑p LTYRNIGsGFVPRGA
Y153 RGAFGKVYLAQDMKT
T290‑p LPKDLRGtEIYMSPE
S334 WVKRYPRSAYPSYLY
A368 MRELIEAALERNPNH
S400‑p EDQPRCQsLDSALFE
S413‑p FERKRLLsRKELQLP
S443‑p EVLRRQRsLYIDLGA
4491 : Phospho-Tpl2 (Ser400) Antibody
  rat

 
S62 QNKEHSESLLRSGQE
T80‑p LSSVRYGtVEDLLAF
S125 NGRYQIDSDVLLVPW
Y136 LVPWKLTYRsIGsGF
S138‑p PWKLTYRsIGsGFVP
S141‑p LTYRsIGsGFVPRGA
Y153 RGAFGKVYLAQDMKT
T290 LPKDLRGTEIYMSPE
S334 WVKRYPRSAYPSYLY
A368 MRELIEAALERNPNH
S400‑p EDQPRCQsLDSALFD
S413 FDRKRLLSRKELELP
S443‑p EVLRRQRsLYIDLGA
4491 : Phospho-Tpl2 (Ser400) Antibody
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