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Protein Page:
MKK4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
MKK4 dual specificity kinase of the STE7 family that phosphorylates and activates JNK1 and -2 as well as p38 but not ERK1 or -2. Mediates cellular responses to various cellular stresses and inflammatory cytokines. Phosphorylation by Akt inhibits MKK4 and suppresses stress-activated signal transduction. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Protein kinase, STE; Kinase, protein; STE group; STE7 family
Chromosomal Location of Human Ortholog: 17p12
Cellular Component: cytoplasm; cytosol
Molecular Function: protein binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: JNK cascade; signal transduction
Reference #:  P45985 (UniProtKB)
Alt. Names/Synonyms: c-Jun N-terminal kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 4; JNK activating kinase 1; JNK-activated kinase 1; JNK-activating kinase 1; JNKK; JNKK1; MAP kinase kinase 4; MAP2K4; MAPK/ERK kinase 4; MAPKK 4; MAPKK4; MEK 4; MEK4; mitogen-activated protein kinase kinase 4; MKK4; MP2K4; PRKMK4; SAPK/ERK kinase 1; SEK1; SERK1
Gene Symbols: MAP2K4
Molecular weight: 44,288 Da
Basal Isoelectric point: 8.28  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  B Cell Receptor Signaling  |  ErbB/HER Signaling  |  GPCR Signaling to MAPKs  |  IL6 Signaling  |  Parkinson's Disease  |  Regulation of P38 MAPKs  |  SAPK/JNK Signaling Cascades  |  T Cell Receptor Signaling  |  TGF-ß Signaling  |  Toll-Like Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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MKK4

Protein Structure Not Found.
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Sites Implicated In
apoptosis, inhibited: S80‑p
transcription, induced: T261‑p
activity, inhibited: S80‑p
enzymatic activity, induced: S257‑p, T261‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 2 S5‑p ___MAAPsPsGGGGS
0 1 S7‑p _MAAPsPsGGGGSGG
0 1 S18‑p GSGGGSGsGTPGPVG
0 3 S26‑p GTPGPVGsPAPGHPA
0 1 K54 NFANPPFKStArFTL
0 4 T56‑p ANPPFKStArFTLNP
0 4 R58‑m1 PPFKStArFTLNPNP
0 4 R58‑m2 PPFKStArFTLNPNP
0 11 T78‑p PHIERLRtHsIESSG
8 96 S80‑p IERLRtHsIESSGKL
0 1 S84 RtHsIESSGKLKIsP
0 21 S90‑p SSGKLKIsPEQHWDF
0 1 T98 PEQHWDFTAEDLKDL
0 1 K103 DFTAEDLKDLGEIGR
0 3 K131‑ac SGQIMAVkRIRSTVD
1 0 K140‑ub IRSTVDEkEQkQLLM
1 0 K143‑ub TVDEkEQkQLLMDLD
0 1 Y191 SFDKFYKYVYSVLDD
0 1 Y193 DKFYKYVYSVLDDVI
0 2 K220 VKALNHLKENLKIIH
14 48 S257‑p ISGQLVDsIAKtRDA
25 3 T261‑p LVDsIAKtRDAGCRP
0 6 T391‑p ILDQMPAtPssPMYV
0 4 S393‑p DQMPAtPssPMYVD_
0 9 S394‑p QMPAtPssPMYVD__
0 1 Y397 AtPssPMYVD_____
9155 : Phospho-SEK1/MKK4 (Ser80) Antibody
4514 : Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb
9156 : Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody
9151 : Phospho-SEK1/MKK4 (Thr261) Antibody
9156 : Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody
  MKK4 iso2  
S5 ___MAAPSPSGGGGS
S7 _MAAPSPSGGGGSGG
S18 GSGGGSGSGTPGPVG
S26 GTPGPVGSPAPGHPA
K65 NFANPPFKSTARFTL
T67 ANPPFKSTARFTLNP
R69 PPFKSTARFTLNPNP
R69 PPFKSTARFTLNPNP
T89 PHIERLRTHSIESSG
S91 IERLRTHSIESSGKL
S95 RTHSIESSGKLKISP
S101 SSGKLKISPEQHWDF
T109 PEQHWDFTAEDLKDL
K114 DFTAEDLKDLGEIGR
K142 SGQIMAVKRIRSTVD
K151 IRSTVDEKEQKQLLM
K154 TVDEKEQKQLLMDLD
Y202 SFDKFYKYVYSVLDD
Y204 DKFYKYVYSVLDDVI
K231 VKALNHLKENLKIIH
S268 ISGQLVDSIAKTRDA
T272 LVDSIAKTRDAGCRP
T402 ILDQMPATPSSPMYV
S404 DQMPATPSSPMYVD_
S405 QMPATPSSPMYVD__
Y408 ATPSSPMYVD_____
  mouse

 
S5 ___MAAPSPSGGGGS
S7 _MAAPSPSGGGGSGG
G16 GGGSGGGGGTPGPIG
P24 GTPGPIGPPASGHPA
K52‑ub NFANPPVkStArFTL
T54‑p ANPPVkStArFTLNP
R56 PPVkStARFTLNPNT
R56‑m2 PPVkStArFTLNPNT
T76 PHIERLRTHsIESsG
S78‑p IERLRTHsIESsGKL
S82‑p RTHsIESsGKLKIsP
S88‑p SsGKLKIsPEQHWDF
T96‑p PEQHWDFtAEDLkDL
K101‑ac DFtAEDLkDLGEIGR
K129 SGQIMAVKRIRSTVD
K138 IRSTVDEKEQKQLLM
K141 TVDEKEQKQLLMDLD
Y189 SFDKFYKYVYSVLDD
Y191 DKFYKYVYSVLDDVI
K218‑ac VKALNHLkENLKIIH
S255‑p ISGQLVDsIAKtRDA
T259‑p LVDsIAKtRDAGCRP
T389‑p ILDQMPAtPssPMyV
S391‑p DQMPAtPssPMyVD_
S392‑p QMPAtPssPMyVD__
Y395‑p AtPssPMyVD_____
9155 : Phospho-SEK1/MKK4 (Ser80) Antibody
4514 : Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb
9156 : Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody
9151 : Phospho-SEK1/MKK4 (Thr261) Antibody
9156 : Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody
  rat

 
S5 ___MAAPSPSGGGGS
S7 _MAAPSPSGGGGSGG
G16 GGGSGGGGGTPGPIG
P24 GTPGPIGPPASGHPA
K52 NFANPPVKSTArFTL
T54 ANPPVKSTArFTLNP
R56‑m1 PPVKSTArFTLNPNT
R56 PPVKSTARFTLNPNT
T76 PHIERLRTHSIESSG
S78 IERLRTHSIESSGKL
S82 RTHSIESSGKLKIsP
S88‑p SSGKLKIsPEQHWDF
T96 PEQHWDFTAEDLKDL
K101 DFTAEDLKDLGEIGR
K129‑ac SGQIMAVkRIRSTVD
K138 IRSTVDEKEQKQLLM
K141 TVDEKEQKQLLMDLD
Y189‑p SFDKFYKyVySVLDD
Y191‑p DKFYKyVySVLDDVI
K218‑ac VKALNHLkENLKIIH
S255 ISGQLVDSIAKtRDA
T259‑p LVDSIAKtRDAGCRP
T389‑p ILDQMPAtPSSPMYV
S391 DQMPAtPSSPMYVD_
S392 QMPAtPSSPMYVD__
Y395 AtPSSPMYVD_____
4514 : Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb
9156 : Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody
9151 : Phospho-SEK1/MKK4 (Thr261) Antibody
9156 : Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody
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