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Protein Page:
Pyk2 (human)
rdtyret
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Pyk2 a nonreceptor tyrosine kinase of the Fak family. Predominantly expressed in the cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for G-protein-coupled receptors. Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. It plays an important role in cell motility such as spreading and migration. Two alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; Nuclear receptor co-regulator; TK group; Fak family
Chromosomal Location of Human Ortholog: 8p21.1
Cellular Component: axon; cell cortex; cell soma; cytoplasm; cytoskeleton; cytosol; dendrite; extrinsic to internal side of plasma membrane; focal adhesion; growth cone; lamellipodium; lipid raft; N-methyl-D-aspartate selective glutamate receptor complex; nucleoplasm; nucleus; perinuclear region of cytoplasm; postsynaptic density
Molecular Function: 3-phosphoinositide-dependent protein kinase binding; ATP binding; calmodulin-dependent protein kinase activity; N-methyl-D-aspartate selective glutamate receptor activity; non-membrane spanning protein tyrosine kinase activity; protein binding; protein complex binding; protein-tyrosine kinase activity; receptor binding; signal transducer activity
Biological Process: activation of JAK protein; adaptive immune response; angiogenesis; apoptosis; blood vessel endothelial cell migration; bone resorption; cell adhesion; cell surface receptor linked signal transduction; cellular defense response; cytoskeleton organization and biogenesis; elevation of cytosolic calcium ion concentration; epidermal growth factor receptor signaling pathway; focal adhesion formation; glial cell proliferation; innate immune response; integrin-mediated signaling pathway; ionotropic glutamate receptor signaling pathway; MAPKKK cascade; marginal zone B cell differentiation; negative regulation of apoptosis; negative regulation of bone mineralization; negative regulation of cell proliferation; negative regulation of myeloid cell differentiation; negative regulation of neuron apoptosis; negative regulation of potassium ion transport; neurite development; oocyte maturation; peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of angiogenesis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of JNK activity; positive regulation of JNK cascade; positive regulation of nitric oxide biosynthetic process; positive regulation of nitric-oxide synthase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein kinase activity; positive regulation of synaptic transmission, glutamatergic; positive regulation of translation; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein complex assembly; regulation of calcium-mediated signaling; regulation of cell adhesion; regulation of cell shape; regulation of cGMP biosynthetic process; regulation of inositol trisphosphate biosynthetic process; regulation of release of sequestered calcium ion into cytosol; response to calcium ion; response to cAMP; response to cocaine; response to drug; response to ethanol; response to glucose stimulus; response to hormone stimulus; response to hydrogen peroxide; response to hypoxia; response to lithium ion; response to mechanical stimulus; response to osmotic stress; response to stress; signal complex assembly; signal transduction; sprouting angiogenesis; stress fiber formation; tumor necrosis factor-mediated signaling pathway; vascular endothelial growth factor receptor signaling pathway
Reference #:  Q14289 (UniProtKB)
Alt. Names/Synonyms: CADTK; CAK beta; CAK-beta; CAKB; Calcium-dependent tyrosine kinase; Cell adhesion kinase beta; FADK 2; FADK2; FAK2; Focal adhesion kinase 2; PKB; Proline-rich tyrosine kinase 2; protein kinase B; protein tyrosine kinase 2 beta; Protein-tyrosine kinase 2-beta; PTK; PTK2B; PTK2B protein tyrosine kinase 2 beta; PYK2; RAFTK; Related adhesion focal tyrosine kinase
Gene Symbols: PTK2B
Molecular weight: 115,875 Da
Basal Isoelectric point: 5.91  Predict pI for various phosphorylation states
CST Pathways:  B Cell Receptor Signaling  |  GPCR Signaling to MAPKs  |  Growth And Differentiation Control by MAPKs  |  Tyrosine Kinases & Substrates
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Pyk2

Protein Structure Not Found.


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Sites Implicated In
cell cycle regulation: Y402‑p
cell motility, altered: Y402‑p
enzymatic activity, induced: Y402‑p
intracellular localization: Y402‑p
molecular association, regulation: Y402‑p, Y881‑p, Y906‑p
phosphorylation: Y402‑p

Modification Sites and Domains  
Click here to view other types of protein modifications

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S2‑p ______MsGVSEPLs
0 1 S9‑p sGVSEPLsRVKLGtL
0 11 T15‑p LsRVKLGtLRRPEGP
0 8 Y46 RILKVCFYSNSFNPG
0 1 S47 ILKVCFYSNSFNPGK
0 1 T150 SLKEDRTTLLYFYQQ
0 1 Y153 EDRTTLLYFYQQLRN
0 1 K188‑ub LELRRFFkDMPHNAL
0 2 K206‑ub SNFELLEkEVGLDLF
0 1 S307 AEFKQIRSIRCLPLE
0 1 S332‑p EGAPQALsIKTSSLA
0 2 Y351 MADLIDGYCRLQGEH
0 1 S361‑p LQGEHQGsLIIHPRK
0 46 S375‑p KDGEKRNsLPQIPML
0 1 M381 NsLPQIPMLNLEARR
0 1 S394‑p RRSHLSEsCsIEsDI
0 2 S396‑p SHLSEsCsIEsDIyA
0 2 S399‑p SEsCsIEsDIyAEIP
66 25 Y402‑p CsIEsDIyAEIPDEt
0 2 T409‑p yAEIPDEtLRRPGGP
0 4 Y418‑p RRPGGPQyGIAREDV
0 10 Y440‑p EGFFGEVyEGVYTNH
0 3 Y444 GEVyEGVYTNHKGEK
0 1 S473‑p DNKEKFMsEAVIMKN
0 3 S530‑p VLTLVLYsLQICKAM
0 1 S559‑p VRNILVAsPECVkLG
0 3 K564‑ub VAsPECVkLGDFGLs
0 3 K564‑ac VAsPECVkLGDFGLs
0 10 S571‑p kLGDFGLsRyIEDED
0 2 Y573‑p GDFGLsRyIEDEDyy
6 963 Y579‑p RyIEDEDyyKAsVTR
15 980 Y580‑p yIEDEDyyKAsVTRL
0 2 S583‑p DEDyyKAsVTRLPIK
0 2 Y683‑p VCSLSDVyQMEKDIA
0 2 Y699‑p EQERNARyRtPKILE
0 1 T701‑p ERNARyRtPKILEPT
0 1 S718‑p QEPPPKPsRPKyRPP
0 9 Y722‑p PKPsRPKyRPPPQTN
0 1 S746‑p VPEGLCAssPtLTsP
0 4 S747‑p PEGLCAssPtLTsPM
0 1 T749‑p GLCAssPtLTsPMEy
0 3 S752‑p AssPtLTsPMEyPsP
0 3 Y756‑p tLTsPMEyPsPVNsL
0 5 S758‑p TsPMEyPsPVNsLHt
0 4 S762‑p EyPsPVNsLHtPPLH
0 7 T765‑p sPVNsLHtPPLHRHN
1 8 S778‑p HNVFKRHsMREEDFI
0 39 Y819‑p QKQMVEDyQWLRQEE
0 1 S828‑p WLRQEEKsLDPMVyM
0 7 Y834‑p KsLDPMVyMNDKsPL
0 7 S839‑p MVyMNDKsPLtPEKE
0 22 T842‑p MNDKsPLtPEKEVGy
0 16 Y849‑p tPEKEVGyLEFtGPP
0 1 L850 PEKEVGyLEFtGPPQ
0 1 T853‑p EVGyLEFtGPPQkPP
0 3 K858‑ub EFtGPPQkPPRLGAQ
0 1 S866‑p PPRLGAQsIQPTANL
17 1 Y881‑p DRTDDLVyLNVMELV
1 0 Y906‑p CQLPPEGyVVVVkNV
0 3 K911‑ub EGyVVVVkNVGLTLR
0 1 S923‑p TLRKLIGsVDDLLPS
0 1 K944‑ub TEIEGTQkLLNKDLA
0 1 K956 DLAELINKMRLAQQN
0 1 K973‑ub TSLSEECkRQMLTAS
3291 : Phospho-Pyk2 (Tyr402) Antibody
  Pyk2 iso2  
S2 ______MSGVSEPLS
S9 SGVSEPLSRVKLGTL
T15 LSRVKLGTLRRPEGP
Y46 RILKVCFYSNSFNPG
S47 ILKVCFYSNSFNPGK
T150 SLKEDRTTLLYFYQQ
Y153 EDRTTLLYFYQQLRN
K188 LELRRFFKDMPHNAL
K206 SNFELLEKEVGLDLF
S307 AEFKQIRSIRCLPLE
S332 EGAPQALSIKTSSLA
Y351 MADLIDGYCRLQGEH
S361 LQGEHQGSLIIHPRK
S375 KDGEKRNSLPQIPML
M381 NSLPQIPMLNLEARR
S394 RRSHLSESCSIESDI
S396 SHLSESCSIESDIYA
S399 SESCSIESDIYAEIP
Y402 CSIESDIYAEIPDET
T409 YAEIPDETLRRPGGP
Y418 RRPGGPQYGIAREDV
Y440 EGFFGEVYEGVYTNH
Y444 GEVYEGVYTNHKGEK
S473 DNKEKFMSEAVIMKN
S530 VLTLVLYSLQICKAM
S559 VRNILVASPECVKLG
K564 VASPECVKLGDFGLS
K564 VASPECVKLGDFGLS
S571 KLGDFGLSRYIEDED
Y573 GDFGLSRYIEDEDYY
Y579 RYIEDEDYYKASVTR
Y580 YIEDEDYYKASVTRL
S583 DEDYYKASVTRLPIK
Y683 VCSLSDVYQMEKDIA
Y699 EQERNARYRTPKILE
T701 ERNARYRTPKILEPT
S718 QEPPPKPSRPKYRPP
Y722 PKPSRPKYRPPPQTN
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
Y777 QKQMVEDYQWLRQEE
S786 WLRQEEKSLDPMVYM
Y792 KSLDPMVYMNDKSPL
S797 MVYMNDKSPLTPEKE
T800 MNDKSPLTPEKEVGY
Y807 TPEKEVGYLEFTGPP
L808 PEKEVGYLEFTGPPQ
T811 EVGYLEFTGPPQKPP
K816 EFTGPPQKPPRLGAQ
S824 PPRLGAQSIQPTANL
Y839 DRTDDLVYLNVMELV
Y864 CQLPPEGYVVVVKNV
K869 EGYVVVVKNVGLTLR
S881 TLRKLIGSVDDLLPS
K902 TEIEGTQKLLNKDLA
K914 DLAELINKMRLAQQN
K931 TSLSEECKRQMLTAS
  mouse

 
S2 ______MSGVSEPLS
S9 SGVSEPLSRVKVGTL
T15 LSRVKVGTLRRPEGP
Y46 RILKVCFYSNSFNPG
S47 ILKVCFYSNSFNPGK
T150 SLKEDRTTLLYFYQQ
Y153 EDRTTLLYFYQQLRN
K188 LELRRFFKDMPHNAL
K206 SNFELLEKEVGLDLF
S307 AEFKQIKSIRCLPLE
S332 EGAPQSLSIKTSSLA
Y351 MADLIDGYCRLQGEH
S361 LQGEHKGSLIMHAKK
S375‑p KDGEKRNsLPQIPtL
T381‑p NsLPQIPtLNLEARR
S394 RRSHLSESCSIESDI
S396 SHLSESCSIESDIyA
S399 SESCSIESDIyAEIP
Y402‑p CSIESDIyAEIPDET
T409 yAEIPDETLRRPGGP
Y418 RRPGGPQYGVAREEV
Y440‑p EGFFGEVyEGVYTNH
Y444 GEVyEGVYTNHKGEK
S473 DNKEKFMSEAVIMKN
T530 VPTLVLYTLQICKAM
S559 VRNILVASPECVKLG
K564 VASPECVKLGDFGLs
K564 VASPECVKLGDFGLs
S571‑p KLGDFGLsRyIEDED
Y573‑p GDFGLsRyIEDEDyy
Y579‑p RyIEDEDyyKASVTR
Y580‑p yIEDEDyyKASVTRL
S583 DEDyyKASVTRLPIK
Y683 VCSLSDIYQMEKDIA
Y699 EQERNARYRPPKILE
P701 ERNARYRPPKILEPT
S718 QEPPPKPSRPKYRPP
Y722 PKPSRPKYRPPPQTN
S746 VPEGLCASsPTLTSP
S747‑p PEGLCASsPTLTSPM
T749 GLCASsPTLTSPMEy
S752 ASsPTLTSPMEyPsP
Y756‑p TLTSPMEyPsPVNSL
S758‑p TSPMEyPsPVNSLHt
S762 EyPsPVNSLHtPPLH
T765‑p sPVNSLHtPPLHRHN
S778‑p HNVFKRHsMREEDFI
S819 QKQMVEDSQWLRREE
C828 WLRREERCLDPMVYM
Y834 RCLDPMVYMNDKSPL
S839 MVYMNDKSPLtPEKE
T842‑p MNDKSPLtPEKEAGy
Y849‑p tPEKEAGytEFTGPP
T850‑p PEKEAGytEFTGPPQ
T853 EAGytEFTGPPQKPP
K858 EFTGPPQKPPRLGAQ
S866 PPRLGAQSIQPTANL
Y881‑p DRTDDLVyHNVMTLV
Y906 GQLPPEDYVVVVKNV
K911 EDYVVVVKNVGLNLR
S923 NLRKLIGSVDDLLPS
K944 TEIEGTQKLLNKDLA
K956‑ac DLAELINkMKLAQQN
K973 TSLSEDCKRQMLTAS
3291 : Phospho-Pyk2 (Tyr402) Antibody
  rat

 
S2 ______MSGVSEPLS
S9 SGVSEPLSRVKVGTL
T15 LSRVKVGTLRPPEGP
Y46‑p RILKVCFysNSFNPG
S47‑p ILKVCFysNSFNPGK
T150‑p SLKEDRTtLLyFYQQ
Y153‑p EDRTtLLyFYQQLRN
K188 LELRRFFKDMPHNAL
K206 SNFELLEKEVGLDLF
S307‑p AEFKQIRsIRCLPLE
S332 EGAPQSLSIKTSSLA
Y351‑p MADLIDGyCRLQGEH
S361 LQGEHKGSLIIHAKK
S375‑p KDGEKRNsLPQIPTL
T381 NsLPQIPTLNLESRR
S394 RRSHLSESCSIESDI
S396 SHLSESCSIESDIyA
S399 SESCSIESDIyAEIP
Y402‑p CSIESDIyAEIPDET
T409 yAEIPDETLRRPGGP
Y418‑p RRPGGPQyGVAREDV
Y440‑p EGFFGEVyEGVyTNH
Y444‑p GEVyEGVyTNHKGEK
S473 DNKEKFMSEAVIMKN
A530 VPTLVLYALQICKAM
S559 VRNILVASPECVKLG
K564 VASPECVKLGDFGLs
K564 VASPECVKLGDFGLs
S571‑p KLGDFGLsRYIEDED
Y573 GDFGLsRYIEDEDyy
Y579‑p RYIEDEDyyKASVTR
Y580‑p YIEDEDyyKASVTRL
S583 DEDyyKASVTRLPIK
Y683 VCSLSDIYQMERDIA
Y699 EQERNARYRPPKILE
P701 ERNARYRPPKILEPT
S718 QEPPPKPSRPKYKHP
Y722 PKPSRPKYKHPPQTN
S746 VPEGLCASSPTLTSP
S747 PEGLCASSPTLTSPM
T749 GLCASSPTLTSPMEY
S752 ASSPTLTSPMEYPSP
Y756 TLTSPMEYPSPVNSL
S758 TSPMEYPSPVNSLHT
S762 EYPSPVNSLHTPPLH
T765 SPVNSLHTPPLHRHN
S778 HNVFKRHSMREEDFI
S819 QKQMVEDSQWLRREE
C828 WLRREERCLDPMVyM
Y834‑p RCLDPMVyMNDKsPL
S839‑p MVyMNDKsPLTPEKE
T842 MNDKsPLTPEKEAGy
Y849‑p TPEKEAGyTEFTGPP
T850 PEKEAGyTEFTGPPQ
T853 EAGyTEFTGPPQKPP
K858 EFTGPPQKPPRLGAQ
S866 PPRLGAQSIQPTANL
Y881‑p DRTDDLVyHNVMTLV
Y906 SQLPPEEYVVVVKNV
K911 EEYVVVVKNVGLNLR
S923 NLRKLIGSVDDLLPS
K944 TEIEGTQKLLNKDLA
K956 DLAELINKMRLAQQN
K973 TSLSEDCKRQMLTAS
3291 : Phospho-Pyk2 (Tyr402) Antibody
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