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Protein Page:
PLK1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PLK1 a kinase of the PLK family. Contains a polo-box domain (PBD), a specific phosphoserine or phosphothreonine binding domain. Substrates include BRCA2, Myt1, NudC, Cdc25C, cyclin B1, Nlp and other mitotic proteins. Inhibited by ATR. Plays a role in regulation of cytokinesis and coordinating M-phase events. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.21; Other group; PLK family
Chromosomal Location of Human Ortholog: 16p12.2
Cellular Component: centrosome; cytoplasm; cytosol; kinetochore; microtubule cytoskeleton; midbody; nucleolus; nucleoplasm; nucleus; spindle; spindle microtubule; spindle midzone; spindle pole
Molecular Function: kinase activity; microtubule binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity
Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell proliferation; centrosome organization and biogenesis; cytokinesis; cytokinesis after mitosis; establishment of protein localization; G2/M transition DNA damage checkpoint; G2/M transition of mitotic cell cycle; microtubule bundle formation; mitosis; mitotic cell cycle spindle assembly checkpoint; mitotic nuclear envelope disassembly; mitotic sister chromatid segregation; negative regulation of apoptosis; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of proteolysis; positive regulation of ubiquitin-protein ligase activity; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein amino acid phosphorylation; protein destabilization; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of cell cycle; regulation of mitotic cell cycle; regulation of mitotic metaphase/anaphase transition; regulation of protein binding; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; sister chromatid cohesion
Reference #:  P53350 (UniProtKB)
Alt. Names/Synonyms: cell cycle regulated protein kinase; PLK; PLK-1; PLK1; polo (Drosophia)-like kinase; polo like kinase; Polo-like kinase 1; polo-like kinase 1 (Drosophila); Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13
Gene Symbols: PLK1
Molecular weight: 68,255 Da
Basal Isoelectric point: 9.09  Predict pI for various phosphorylation states
CST Pathways:  G2/M DNA Damage Checkpoint
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PLK1

Protein Structure Not Found.
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Download ChimeraX Script

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
apoptosis, inhibited: S137‑p
cell cycle regulation: S49‑p, S137‑p, T210‑p, T214‑p, S326‑p, S330‑p, S597‑p
cell growth, induced: S137‑p, T210‑p, T214‑p
enzymatic activity, induced: S49‑p, S137‑p, T210‑p, T214‑p, S326‑p
intracellular localization: T210‑p
molecular association, regulation: T210‑p, S330‑p, S597‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 3 S2‑p ______MsAAVtAGk
0 3 T6‑p __MsAAVtAGkLARA
0 2 K9‑ac sAAVtAGkLARAPAD
0 1 K9‑ub sAAVtAGkLARAPAD
0 1 K19‑ub RAPADPGkAGVPGVA
1 1 A34 APGAPAAAPPAkEIP
1 1 P35 PGAPAAAPPAkEIPE
0 3 K38‑ub PAAAPPAkEIPEVLV
1 0 S49‑p EVLVDPRsRRRYVRG
0 1 K61‑ub VRGRFLGkGGFAKCF
0 1 K91‑ub VPKSLLLkPHQREKM
0 1 S103‑p EKMSMEIsIHRSLAH
5 1 S137‑p LELCRRRsLLELHKR
0 1 K146‑ub LELHKRRkALTEPEA
1 0 Y166 QIVLGCQYLHRNRVI
0 2 K200‑ub GDFGLATkVEYDGER
28 65 T210‑p YDGERKKtLCGtPNy
1 20 T214‑p RKKtLCGtPNyIAPE
0 148 Y217‑p tLCGtPNyIAPEVLs
0 4 S224‑p yIAPEVLsKKGHSFE
0 2 K272‑ub KNEYSIPkHINPVAA
0 1 Y309‑p DEFFTSGyIPARLPI
1 2 S326‑p LTIPPRFsIAPssLD
2 0 S330‑p PRFsIAPssLDPsNR
0 3 S331‑p RFsIAPssLDPsNRk
1 2 S335‑p APssLDPsNRkPLtV
0 2 K338‑ub sLDPsNRkPLtVLNK
0 2 T341‑p PsNRkPLtVLNKGLE
1 1 N349 VLNKGLENPLPERPR
0 1 K358‑ub LPERPREkEEPVVRE
0 1 T366‑p EEPVVREtGEVVDCH
0 3 S375‑p EVVDCHLsDMLQQLH
1 2 S383‑p DMLQQLHsVNAsKPS
0 1 S387‑p QLHsVNAsKPSERGL
1 0 Y445 DSTRLILYNDGDsLQ
0 3 S450‑p ILYNDGDsLQYIERD
0 1 T459‑p QYIERDGtEsyLtVs
0 2 S461‑p IERDGtEsyLtVsSH
0 1 Y462‑p ERDGtEsyLtVsSHP
0 1 T464‑p DGtEsyLtVsSHPNS
0 1 S466‑p tEsyLtVsSHPNSLM
0 1 K474‑ub SHPNSLMkKITLLkY
0 1 K480‑ub MkKITLLkYFRNYMS
1 2 K492‑ub YMSEHLLkAGANItP
0 4 T498‑p LkAGANItPREGDEL
0 1 S565‑p DFRTYRLsLLEEYGC
0 1 K574‑ub LEEYGCCkELAsRLR
0 1 S578‑p GCCkELAsRLRYART
0 2 K589‑ub YARTMVDkLLSSRSA
2 0 S597‑p LLSSRSAsNRLKAS_
4354 : Phospho-PLK1 (Thr210) Antibody (ELISA-Specific)
5472 : Phospho-PLK1 (Thr210) Antibody
9062 : Phospho-PLK1 (Thr210) (D5H7) Rabbit mAb
  mouse

 
N2 ______MNAAAKAGK
K6 __MNAAAKAGKLARA
K9 NAAAKAGKLARAPAD
K9 NAAAKAGKLARAPAD
K19 RAPADLGKGGVPGDA
A34 VPGAPVAAPLAKEIP
P35 PGAPVAAPLAKEIPE
K38 PVAAPLAKEIPEVLV
S49 EVLVDPRSRRQYVRG
K61 VRGRFLGKGGFAKCF
K91 VPKSLLLKPHQKEKM
S103 EKMSMEISIHRSLAH
S137 LELCRRRSLLELHKR
K146 LELHKRRKALTEPEA
Y166‑p QIVLGCQyLHRNQVI
K200 GDFGLATKVEYEGER
T210‑p YEGERKKtLCGtPNY
T214‑p RKKtLCGtPNYIAPE
Y217 tLCGtPNYIAPEVLS
S224 YIAPEVLSKKGHSFE
K272 KNEYSIPKHINPVAA
Y309 DEFFTSGYIPARLPI
S326 LTIPPRFSIAPSSLD
S330 PRFSIAPSSLDPSSR
S331 RFSIAPSSLDPSSRK
S335 APSSLDPSSRKPLKV
K338 SLDPSSRKPLKVLNK
K341 PSSRKPLKVLNKGVE
N349 VLNKGVENPLPDRPR
K358 LPDRPREKEEPVVRE
T366 EEPVVRETNEAIECH
S375 EAIECHLSDLLQQLT
S383 DLLQQLTSVNASKPS
S387 QLTSVNASKPSERGL
Y445‑p DSTRLILyNDGDSLQ
S450 ILyNDGDSLQYIERD
T459 QYIERDGTESYLTVS
S461 IERDGTESYLTVSSH
Y462 ERDGTESYLTVSSHP
T464 DGTESYLTVSSHPNS
S466 TESYLTVSSHPNSLM
K474 SHPNSLMKKITLLNY
N480 MKKITLLNYFRNYMS
K492 YMSEHLLKAGANITP
T498 LKAGANITPREGDEL
S565 DFQTYRLSLLEEYGC
K574 LEEYGCCKELASRLR
S578 GCCKELASRLRYART
K589 YARTMVDKLLSSRSA
S597 LLSSRSASNRLKAS_
4354 : Phospho-PLK1 (Thr210) Antibody (ELISA-Specific)
5472 : Phospho-PLK1 (Thr210) Antibody
  rat

 
N2 ______MNAAAKAGK
K6 __MNAAAKAGKLARA
K9 NAAAKAGKLARAPAD
K9 NAAAKAGKLARAPAD
K19 RAPADLGKGGVPGDA
A34 APGAPGAAPLAKEIP
P35 PGAPGAAPLAKEIPE
K38 PGAAPLAKEIPEVLM
S49 EVLMDPRSRRQYVRG
K61 VRGRFLGKGGFAKCF
K91 VPKSLLLKPHQKEKM
S103 EKMSMEISIHRSLAH
S137 LELCRRRSLLELHKR
K146 LELHKRRKALTEPEA
Y166 QIVLGCQYLHRNQVI
K200 GDFGLATKVEYEGER
T210‑p YEGERKKtLCGTPNY
T214 RKKtLCGTPNYIAPE
Y217 tLCGTPNYIAPEVLS
S224 YIAPEVLSKKGHSFE
K272 KNEYSIPKHINPVAA
Y309 DEFFTSGYIPARLPI
S326 LTIPPRFSIAPSSLD
S330 PRFSIAPSSLDPSNR
S331 RFSIAPSSLDPSNRK
S335 APSSLDPSNRKPLTV
K338 SLDPSNRKPLTVLNK
T341 PSNRKPLTVLNKGVE
N349 VLNKGVENPLPDRPR
K358 LPDRPREKEEPVVRE
T366 EEPVVRETNEAIECH
S375 EAIECHLSDLLQQLT
S383 DLLQQLTSVNASKPS
S387 QLTSVNASKPSERGL
Y445 DSTRLILYNDGDSLQ
S450 ILYNDGDSLQYIERD
T459 QYIERDGTESYLTVS
S461 IERDGTESYLTVSSH
Y462 ERDGTESYLTVSSHP
T464 DGTESYLTVSSHPNS
S466 TESYLTVSSHPNSLM
K474 SHPNSLMKKITLLNY
N480 MKKITLLNYFRNYMS
K492 YMSEHLLKAGANITP
T498 LKAGANITPREGDEL
S565 DFRTYRLSLLEEYGC
K574 LEEYGCCKELASRLR
S578 GCCKELASRLRYART
K589 YARTMVDKLLSSRSA
C597 LLSSRSACNRLKAS_
4354 : Phospho-PLK1 (Thr210) Antibody (ELISA-Specific)
5472 : Phospho-PLK1 (Thr210) Antibody
  frog

 
- gap
- gap
K8 MAQVAGKKLTVAPEA
K8 MAQVAGKKLTVAPEA
- gap
S25‑p PPGIPGSssAVKEIP
S26‑p PGIPGSssAVKEIPE
K29 PGSssAVKEIPEILV
T40 EILVDPRTRRRYLRG
K52 LRGRFLGKGGFAKCY
K82 VPKTMLLKPHQKDKM
A94 DKMTMEIAIQRSLDH
S128 LELCRRRSLLELHKR
K137 LELHKRRKAVTEPEA
Y157 QTISGCQYLHSNRVI
K191 GDFGLATKVEYDGER
T201 YDGERKKTLCGTPNY
T205 RKKTLCGTPNYIAPE
Y208 TLCGTPNYIAPEVLG
G215 YIAPEVLGKKGHSFE
K263 KNEYSIPKHINPVAA
Y300 DEFFTSGYIPSRLPT
S317 LTVPPRFSIAPSTID
S321 PRFSIAPSTIDQsLR
T322 RFSIAPSTIDQsLRK
S326‑p APSTIDQsLRKPLTA
K329 TIDQsLRKPLTAINK
T332 QsLRKPLTAINKGQD
S340‑p AINKGQDsPLVEKQV
E352 KQVAPAKEEEMQQPE
T361 EMQQPEFTEPADCYL
S369 EPADCYLSEMLQQLT
C377 EMLQQLTCLNAVKPS
V381 QLTCLNAVKPSERAL
Y439 DSTRLIMYNDGDSLQ
S444 IMYNDGDSLQYIERN
T453 QYIERNNTESYLNVR
S455 IERNNTESYLNVRSY
Y456 ERNNTESYLNVRSYP
N458 NNTESYLNVRSYPTT
R460 TESYLNVRSYPTTLT
K468 SYPTTLTKKITLLKY
K474 TKKITLLKYFRNYMS
K486 YMSEHLLKAGANTTP
T492 LKAGANTTPREGDEL
S559 EFRTYKLSLIQEFGC
K568 IQEFGCCKELASRLR
S572 GCCKELASRLRYART
K583 YARTMVEKLQSSKSA
V591 LQSSKSAVAHVKASA
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