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Protein Page:
PKCH (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PKCH Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-p27Kip1 complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1- dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Interacts with FYN and RALA. Interacts with DGKQ. Most abundant in lung, less in heart and skin. Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-513 (activation loop of the kinase domain), Thr-656 (turn motif) and Ser-675 (hydrophobic region), need to be phosphorylated for its full activation. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, AGC; Kinase, protein; EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); AGC group; PKC family; Eta subfamily
Chromosomal Location of Human Ortholog: 14q23.1
Cellular Component: cytoplasm; cytosol; intercellular junction; plasma membrane
Molecular Function: ATP binding; calcium-independent protein kinase C activity; enzyme binding; metal ion binding; protein kinase C activity; Ral GTPase binding
Biological Process: activation of NF-kappaB transcription factor; peptidyl-serine phosphorylation; platelet activation; positive regulation of B cell receptor signaling pathway; positive regulation of keratinocyte differentiation; protein amino acid phosphorylation; signal transduction
Disease: Stroke, Ischemic
Reference #:  P24723 (UniProtKB)
Gene Symbols: PRKCH
Molecular weight: 77,828 Da
Basal Isoelectric point: 7.94  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  B Cell Receptor Signaling  |  ErbB/HER Signaling  |  GPCR Signaling to MAPKs  |  Growth And Differentiation Control by MAPKs  |  Inhibition of Apoptosis  |  Mitochondrial Control of Apoptosis  |  Phospholipase Signaling
Select Structure to View Below

PKCH

Protein Structure Not Found.
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Substrate Sequence Logo
Sequence Logo

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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 T25‑p EAVGLQPtRWsLRHs
2 17 S28‑p GLQPtRWsLRHsLFK
2 10 S32‑p tRWsLRHsLFKkGHQ
0 1 K35 sLRHsLFKkGHQLLD
0 2 K36‑ub LRHsLFKkGHQLLDP
0 5 Y94‑p FHETPLGyDHFVANC
0 4 T183‑p ATYLRQPtYCSHCRE
0 1 K234‑ub NINKVDSkIAEQRFG
0 2 S249‑p INIPHKFsIHNYKVP
0 2 T305 NAVELAKTLAGMGLQ
1 9 S317‑p GLQPGNIsPTskLVs
0 1 T319 QPGNIsPTskLVsRs
0 2 S320‑p PGNIsPTskLVsRsT
0 2 K321‑ub GNIsPTskLVsRsTL
0 1 S324‑p sPTskLVsRsTLRRQ
1 2 S326‑p TskLVsRsTLRRQGK
0 1 K337‑ub RQGKESSkEGNGIGV
0 2 S365‑p IRVLGKGsFGKVMLA
0 16 Y381 VKETGDLYAVKVLKK
0 1 K404‑ub VECTMTEkRILSLAR
0 2 R478 HDKGIIYRDLKLDNV
1 1 T513‑p CNGVTTAtFCGTPDY
0 1 S599‑p NPTMRLGsLtQGGEH
0 1 T601‑p TMRLGsLtQGGEHAI
1 13 T656‑p IKEEPVLtPIDEGHL
1 11 S675‑p QDEFRNFsyVSPELQ
0 2 Y676‑p DEFRNFsyVSPELQP
2060 : Phospho-PKC (pan) (zeta Thr410) (190D10) Rabbit mAb
5016 : Phospho-PKC (pan) (zeta Thr410) (190D10) Rabbit mAb (Biotinylated)
9379 : Phospho-PKC (pan) (gamma Thr514) Antibody
38938 : Phospho-PKC (pan) (gamma Thr514) (D6Y3D) Rabbit mAb
9371 : Phospho-PKC (pan) (betaII Ser660) Antibody
  mouse

 
T25‑p EAVGLQPtRWsLRHs
S28‑p GLQPtRWsLRHsLFk
S32‑p tRWsLRHsLFkKGHQ
K35‑ac sLRHsLFkKGHQLLD
K36 LRHsLFkKGHQLLDP
Y94 FHETPLGYDHFVANC
T183 ATYLRQPTYCSHCRE
K234 NINKVDAKIAEQRFG
N249 INIPHKFNVHNYKVP
T305‑p NAVELAKtLAGMGLQ
S317‑p GLQPGNIsPtsKLIS
T319‑p QPGNIsPtsKLISRs
S320‑p PGNIsPtsKLISRsT
K321 GNIsPtsKLISRsTL
S324 sPtsKLISRsTLRRQ
S326‑p tsKLISRsTLRRQGK
K337 RQGKEGSKEGNGIGV
S365 IRVLGKGSFGKVMLA
Y381 IKETGELYAVKVLKK
K404 VECTMTEKRILSLAR
R478‑m1 HEKGIIYrDLKLDNV
T513‑p CNGVTTAtFCGTPDY
S599 NPTMRLGSLTQGGEH
T601 TMRLGSLTQGGEHEI
T656‑p IKEEPVLtPIDEGHL
S675‑p QDEFRNFsYVSPELQ
Y676 DEFRNFsYVSPELQL
2060 : Phospho-PKC (pan) (zeta Thr410) (190D10) Rabbit mAb
5016 : Phospho-PKC (pan) (zeta Thr410) (190D10) Rabbit mAb (Biotinylated)
9379 : Phospho-PKC (pan) (gamma Thr514) Antibody
38938 : Phospho-PKC (pan) (gamma Thr514) (D6Y3D) Rabbit mAb
9371 : Phospho-PKC (pan) (betaII Ser660) Antibody
  rat

 
T25 EAVGLQPTRWSLRHS
S28 GLQPTRWSLRHSLFK
S32 TRWSLRHSLFKKGHQ
K35 SLRHSLFKKGHQLLD
K36 LRHSLFKKGHQLLDP
Y94 FHETPLGYDHFVANC
T183 ATYLRQPTYCSHCRE
K234 NINKVDAKIAEQRFG
N249 INIPHKFNVHNYKVP
T305 NAVELAKTLAGMGLQ
S317‑p GLQPGNIsPTSKLIS
T319 QPGNIsPTSKLISRS
S320 PGNIsPTSKLISRST
K321 GNIsPTSKLISRSTL
S324 sPTSKLISRSTLRRQ
S326 TSKLISRSTLRRQGK
K337 RQGKEGSKEGNGIGV
S365 IRVLGKGSFGKVMLA
Y381‑p IKETGELyAVKVLKK
K404 VECTMTEKRILSLAR
R478 HEKGIIYRDLKLDNV
T513 CNGVTTATFCGTPDY
S599 NPTMRLGSLTQGGEH
T601 TMRLGSLTQGGEHEI
T656‑p IKEEPVLtPIDEGHL
S675‑p QDEFRNFsYVSPELQ
Y676 DEFRNFsYVSPELQP
2060 : Phospho-PKC (pan) (zeta Thr410) (190D10) Rabbit mAb
5016 : Phospho-PKC (pan) (zeta Thr410) (190D10) Rabbit mAb (Biotinylated)
9379 : Phospho-PKC (pan) (gamma Thr514) Antibody
38938 : Phospho-PKC (pan) (gamma Thr514) (D6Y3D) Rabbit mAb
9371 : Phospho-PKC (pan) (betaII Ser660) Antibody
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