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Protein Page:
RNF20 (human)

RNF20 Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with p53/TP53 and WAC. Interacts with PAF1; the interaction mediates the association of the PAF1 and RNF20/40 complexes which is a prerequsite for recruitment of UBE2A/B. Belongs to the BRE1 family. Note: This description may include information from UniProtKB.
Protein type: EC 6.3.2.-; EC; Ligase; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 9q22
Cellular Component: nucleolus; nucleoplasm; nucleus; ubiquitin ligase complex
Molecular Function: histone binding; mRNA 3'-UTR binding; p53 binding; protein binding; transcription coactivator activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity
Biological Process: histone H2B ubiquitination; histone monoubiquitination; negative regulation of cell migration; positive regulation of transcription, DNA-dependent; protein polyubiquitination; regulation of mitotic cell cycle; regulation of transcription, DNA-dependent; ubiquitin-dependent protein catabolic process
Reference #:  Q5VTR2 (UniProtKB)
Alt. Names/Synonyms: BRE1; BRE1 E3 ubiquitin ligase homolog; BRE1-A; BRE1A; E3 ubiquitin-protein ligase BRE1A; FLJ11189; FLJ20382; hBRE1; homolog of S. cerevisiae BRE1; KIAA2779; MGC129667; MGC129668; RING finger protein 20; RNF20
Gene Symbols: RNF20
Molecular weight: 113,662 Da
Basal Isoelectric point: 5.73  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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