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Protein Page:
MKK3 (human)
rdtyret
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
MKK3 a dual-specificity protein kinase of the STE7 family. Activates p38 MAP kinase by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by cytokines and environmental stress in vivo. A deletion and two point mutants found in colon cancer cell lines. Three splice-variant isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, STE; Kinase, protein; EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family
Chromosomal Location of Human Ortholog: 17q11.2
Cellular Component: cytoplasm; cytosol; membrane; nucleoplasm
Molecular Function: ATP binding; MAP kinase kinase activity; protein binding; protein kinase binding; protein serine/threonine kinase activity; protein-tyrosine kinase activity
Biological Process: activation of MAPK activity; cardiac muscle contraction; inflammatory response; innate immune response; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; peptidyl-tyrosine phosphorylation; positive regulation of protein kinase activity; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of cytokine biosynthetic process; regulation of mitotic cell cycle; signal transduction; stress-activated MAPK cascade; stress-activated protein kinase signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway
Reference #:  P46734 (UniProtKB)
Alt. Names/Synonyms: Dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; MAP2K3; MAPK/ERK kinase 3; MAPKK 3; MAPKK3; MEK 3; MEK3; mitogen-activated protein kinase kinase 3; MKK3; MP2K3; PRKMK3
Gene Symbols: MAP2K3
Molecular weight: 39,318 Da
Basal Isoelectric point: 7.05  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  B Cell Receptor Signaling  |  ErbB/HER Signaling  |  GPCR Signaling to MAPKs  |  IL6 Signaling  |  Regulation of P38 MAPKs  |  TGF-ß Signaling  |  Toll-Like Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

MKK3

Protein Structure Not Found.


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Sites Implicated In
cell cycle regulation: T222‑p
transcription, induced: S218‑p, T222‑p
activity, induced: T222‑p
enzymatic activity, induced: S218‑p, T222‑p

Modification Sites and Domains  
Click here to view other types of protein modifications

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 6 S3‑p _____MEsPASSQPA
0 3 S15‑p QPASMPQsKGKSKRK
0 2 S28‑p RKKDLRIsCMsKPPA
0 2 S31‑p DLRIsCMsKPPAPNP
0 9 T39‑p KPPAPNPtPPRNLDS
0 6 K80‑ub GAYGVVEkVRHAQSG
0 1 K192‑ub SVIHRDVkPSNVLIN
0 1 K200 PSNVLINKEGHVKMC
0 2 S212‑p KMCDFGIsGyLVDsV
0 3 Y214‑p CDFGIsGyLVDsVAK
14 71 S218‑p IsGyLVDsVAKtMDA
4 3 T222‑p LVDsVAKtMDAGCKP
0 3 Y230‑p MDAGCKPyMAPERIN
0 4 K243‑ub INPELNQkGyNVKsD
0 1 Y245‑p PELNQkGyNVKsDVW
0 2 S249‑p QkGyNVKsDVWsLGI
0 1 S253‑p NVKsDVWsLGITMIE
0 1 K279‑ub GTPFQQLkQVVEEPS
0 1 K340‑ub TDIAAFVkEILGEDS
9231 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody
9236 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) (22A8) Rabbit mAb
12280 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb
  MKK3 iso2  
- gap
- gap
- gap
S2 ______MSKPPAPNP
T10 KPPAPNPTPPRNLDS
K51 GAYGVVEKVRHAQSG
K163 SVIHRDVKPSNVLIN
K171 PSNVLINKEGHVKMC
S183 KMCDFGISGYLVDSV
Y185 CDFGISGYLVDSVAK
S189 ISGYLVDSVAKTMDA
T193 LVDSVAKTMDAGCKP
Y201 MDAGCKPYMAPERIN
K214 INPELNQKGYNVKSD
Y216 PELNQKGYNVKSDVW
S220 QKGYNVKSDVWSLGI
S224 NVKSDVWSLGITMIE
K250 GTPFQQLKQVVEEPS
K311 TDIAAFVKEILGEDS
  MKK3 iso3  
- gap
- gap
S33 RKKDLRISCMSKPPA
S36 DLRISCMSKPPAPNP
T44 KPPAPNPTPPRNLDS
K85 GAYGVVEKVRHAQSG
K197 SVIHRDVKPSNVLIN
K205 PSNVLINKEGHVKMC
S217 KMCDFGISGYLVDSV
Y219 CDFGISGYLVDSVAK
S223 ISGYLVDSVAKTMDA
T227 LVDSVAKTMDAGCKP
Y235 MDAGCKPYMAPERIN
K248 INPELNQKGYNVKSD
Y250 PELNQKGYNVKSDVW
S254 QKGYNVKSDVWSLGI
S258 NVKSDVWSLGITMIE
K284 GTPFQQLKQVVEEPS
K345 TDIAAFVKEILGEDS
  mouse

 
S3 _____MESPAASPPA
T15 PPASLPQTKGKSKRK
S28‑p RKKDLRIsCVsKPPV
S31‑p DLRIsCVsKPPVSNP
T39 KPPVSNPTPPRNLDS
K80‑ub GAYGVVEkVRHAQSG
K192 SVIHRDVKPSNVLIN
K200‑ac PSNVLINkEGHVKMC
S212 KMCDFGISGYLVDsV
Y214 CDFGISGYLVDsVAK
S218‑p ISGYLVDsVAKTMDA
T222 LVDsVAKTMDAGCKP
Y230 MDAGCKPYMAPERIN
K243‑ub INPELNQkGYNVKSD
Y245 PELNQkGYNVKSDVW
S249 QkGYNVKSDVWSLGI
S253 NVKSDVWSLGITMIE
K279 GTPFQQLKQVVEEPS
K340 TDIAAFVKEILGEDS
9231 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody
9236 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) (22A8) Rabbit mAb
12280 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb
  rat

 
S75 ATVRSMESPAASPPA
T87 PPASLPQTKGKSKRK
S100 RKRDLRISCVSKPPV
S103 DLRISCVSKPPVSNP
T111 KPPVSNPTPPRNLDS
K152 GAYGVVEKVRHAQSG
K264 SVIHRDVKPSNVLIN
K272 PSNVLINKEGHVKMC
S284 KMCDFGISGYLVDsV
Y286 CDFGISGYLVDsVAK
S290‑p ISGYLVDsVAKTMDA
T294 LVDsVAKTMDAGCKP
Y302 MDAGCKPYMAPERIN
K315 INPELNQKGYNVKSD
Y317 PELNQKGYNVKSDVW
S321 QKGYNVKSDVWSLGI
S325 NVKSDVWSLGITMIE
K351 GTPFQQLKQVVEEPS
K412 TDIAAFVKEILGEDS
9231 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody
9236 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) (22A8) Rabbit mAb
12280 : Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb
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