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Protein Page:
LKB1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
LKB1 a S/T protein kinase of the CAMKL family. A tumor suppressor that helps control cell structure, polarity, apoptosis and energy homeostasis. Phosphorylates AGS3 (activator of G-protein signaling 3) GPR domains, regulating the interaction of GPR-containing proteins with G-proteins. A cytosolic protein complex comprised of LKB1, the pseudokinase STRAD, and the MO25 scaffold protein. Activates AMPK and several related protein kinases. AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations. The Lkb1/Strad/Mo25 complex can polarize single epithelial cells, leading to the formation of a brush border containing microvilli on the apical surface. Mutation in the corresponding gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands and feet. A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; Autophagy; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor; Protein kinase, CAMK; CAMK group; CAMKL family; LKB subfamily
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: cytoplasm; cytosol; membrane; mitochondrion; nucleoplasm; nucleus
Molecular Function: ATP binding; magnesium ion binding; p53 binding; protein binding; protein kinase activator activity; protein serine/threonine kinase activity
Biological Process: anoikis; cell cycle arrest; establishment of cell polarity; glucose homeostasis; negative regulation of cell growth; negative regulation of cell proliferation; positive regulation of autophagy; positive regulation of transforming growth factor beta receptor signaling pathway; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell growth; response to DNA damage stimulus; response to ionizing radiation; vasculature development
Disease: Pancreatic Cancer; Peutz-jeghers Syndrome; Testicular Germ Cell Tumor
Reference #:  Q15831 (UniProtKB)
Alt. Names/Synonyms: LKB1; PAR-4; PAR4; PJS; polarization-related protein LKB1; Renal carcinoma antigen NY-REN-19; serine/threonine kinase 11; Serine/threonine-protein kinase 11; Serine/threonine-protein kinase LKB1; STK11
Gene Symbols: STK11
Molecular weight: 48,636 Da
Basal Isoelectric point: 7.12  Predict pI for various phosphorylation states
CST Pathways:  AMPK Signaling  |  Insulin Receptor Signaling  |  mTOR Signaling  |  Translation: eIF4E and p70S6K  |  Warburg Effect
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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LKB1

Protein Structure Not Found.
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Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
apoptosis, altered: S307‑p
apoptosis, inhibited: T189‑p
cell growth, altered: S307‑p, S428‑p
enzymatic activity, induced: T185‑p, T336‑p, T363‑p, T402‑p, S428‑p
intracellular localization: S307‑p, S428‑p
molecular association, regulation: S307‑p, S428‑p
phosphorylation: S428‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
1 303 S31‑p TFIHRIDstEVIyQP
0 36 T32‑p FIHRIDstEVIyQPR
1 13 Y36‑p IDstEVIyQPRRKRA
1 0 K44‑ac QPRRKRAkLIGkYLM
1 0 K48‑ac KRAkLIGkYLMGDLL
1 0 Y60 DLLGEGSYGKVKEVL
2 0 K96‑ac PNGEANVkkEIQLLR
2 0 K97‑ac NGEANVkkEIQLLRR
1 0 Y156 PVCQAHGYFCQLIDG
1 0 Y166 QLIDGLEYLHSQGIV
1 0 K178‑sm GIVHKDIkPGNLLLt
1 0 T185‑p kPGNLLLtTGGtLKI
2 0 T189‑p LLLtTGGtLKISDLG
0 1 S240‑p GFKVDIWsAGVTLYN
3 0 Y261 PFEGDNIYKLFENIG
1 0 K296‑ac MLEYEPAkRFSIRQI
2 0 S307‑p IRQIRQHsWFRkKHP
1 0 K311‑ac RQHsWFRkKHPPAEA
1 0 S325 APVPIPPSPDTKDRW
1 0 S334‑p DTKDRWRsMtVVPYL
2 0 T336‑p KDRWRsMtVVPYLED
3 0 Y362 DIEDDIIYtQDFTVP
5 0 T363‑p IEDDIIYtQDFTVPG
1 0 - gap
0 1 S401‑p GTEAAQLstKsRAEG
1 0 T402‑p TEAAQLstKsRAEGR
0 1 S404‑p AAQLstKsRAEGRAP
1 0 K416‑ac RAPNPARkACSASSk
0 1 S421 ARkACSASSkIRRLs
1 0 K423‑ac kACSASSkIRRLsAC
25 5 S428‑p SSkIRRLsACkQQ__
1 0 K431‑ac IRRLsACkQQ_____
3054 : Phospho-LKB1 (Thr189) Antibody
3055 : Phospho-LKB1 (Ser334) Antibody
3482 : Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb
  LKB1 iso2  
S31 TFIHRIDSTEVIYQP
T32 FIHRIDSTEVIYQPR
Y36 IDSTEVIYQPRRKRA
K44 QPRRKRAKLIGKYLM
K48 KRAKLIGKYLMGDLL
Y60 DLLGEGSYGKVKEVL
K96 PNGEANVKKEIQLLR
K97 NGEANVKKEIQLLRR
Y156 PVCQAHGYFCQLIDG
Y166 QLIDGLEYLHSQGIV
K178 GIVHKDIKPGNLLLT
T185 KPGNLLLTTGGTLKI
T189 LLLTTGGTLKISDLG
S240 GFKVDIWSAGVTLYN
Y261 PFEGDNIYKLFENIG
K296 MLEYEPAKRFSIRQI
S307 IRQIRQHSWFRKKHP
K311 RQHSWFRKKHPPAEA
S325 APVPIPPSPDTKDRW
S334 DTKDRWRSMTVVPYL
T336 KDRWRSMTVVPYLED
Y362 DIEDDIIYTQDFTVP
T363 IEDDIIYTQDFTVPG
S399‑p DLSGEEAsRPAPQ__
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  mouse

 
S31‑p TFIHRIDstEVIyQP
T32‑p FIHRIDstEVIyQPR
Y36‑p IDstEVIyQPRRKRA
K44 QPRRKRAKLIGKYLM
K48 KRAKLIGKYLMGDLL
Y60‑p DLLGEGSyGKVKEVL
K96 PNGEANVKKEIQLLR
K97 NGEANVKKEIQLLRR
Y156‑p PVCQAHGyFRQLIDG
Y166‑p QLIDGLEyLHSQGIV
K178 GIVHKDIKPGNLLLT
T185 KPGNLLLTTNGTLKI
T189 LLLTTNGTLKISDLG
S240 GFKVDIWSAGVTLYN
Y261‑p PFEGDNIyKLFENIG
K296 MLEYEPAKRFSIRQI
S307 IRQIRQHSWFRKKHP
K311 RQHSWFRKKHPLAEA
S325‑p ALVPIPPsPDTKDRW
S334 DTKDRWRSMtVVPYL
T336‑p KDRWRSMtVVPYLED
Y365‑p DIEDGIIytQDFTVP
T366‑p IEDGIIytQDFTVPG
- gap
S403 NGTEPQLSSKVKPEG
S404 GTEPQLSSKVKPEGR
V406 EPQLSSKVKPEGRPG
K420 GTANPARKVCSsNKI
S424‑p PARKVCSsNKIRRLs
K426 RKVCSsNKIRRLsAC
S431‑p sNKIRRLsACKQQ__
K434 IRRLsACKQQ_____
3482 : Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb
  rat

 
S31‑p TFIHRIDsTEVIYQP
T32 FIHRIDsTEVIYQPR
Y36 IDsTEVIYQPRRKRA
K44 QPRRKRAKLIGKYLM
K48 KRAKLIGKYLMGDLL
Y60 DLLGEGSYGKVKEVL
K96 PNGEANVKKEIQLLR
K97 NGEANVKKEIQLLRR
Y156 PVCQAHGYFRQLIDG
Y166 QLIDGLEYLHSQGIV
K178 GIVHKDIKPGNLLLT
T185 KPGNLLLTTNGTLKI
T189 LLLTTNGTLKISDLG
S240 GFKVDIWSAGVTLYN
Y261 PFEGDNIYKLFENIG
K296 MLEYEPAKRFSIRQI
S307 IRQIRQHSWFRKKHP
K311 RQHSWFRKKHPLAEA
S325 ALVPIPPSPDTKDRW
S334 DTKDRWRSMTVVPYL
T336 KDRWRSMTVVPYLED
Y365 DIEDGIIYTQDFTVP
T366 IEDGIIYTQDFTVPG
- gap
S403 NGTEPQLSSKVKPEG
S404 GTEPQLSSKVKPEGR
V406 EPQLSSKVKPEGRPG
K420 GAANPARKVCSSNKI
S424 PARKVCSSNKIRRLs
K426 RKVCSSNKIRRLsAC
S431‑p SNKIRRLsACKQQ__
K434 IRRLsACKQQ_____
  pig

 
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
Y62 PVCQAHGYFCQLVDG
Y72 QLVDGLEYLHSQGIV
K84 GIVHKDIKPGNLLLT
T91 KPGNLLLTTGGTLKI
T95 LLLTTGGTLKISDLG
S146 GFKVDIWSAGVTLYN
Y167 PFEGDNIYKLFENIG
K202 MLEYEPAKRFSIQQI
S213 IQQIRQHSWFRKKHP
K217 RQHSWFRKKHPPAEE
S231 EPVPIPPSTDCKDRW
G240 DCKDRWRGMTVVPYL
T242 KDRWRGMTVVPYLED
Y270 DIEDDIIYTQDFTVP
T271 IEDDIIYTQDFTVPG
- gap
S309 GTESVQLSAKSRAER
A310 TESVQLSAKSRAERR
S312 SVQLSAKSRAERRAS
K327 ATSNPTRKACSASSK
S332 TRKACSASSKIRRLs
K334 KACSASSKIRRLsAC
S339‑p SSKIRRLsACKQQ__
K342 IRRLsACKQQ_____
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