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Protein Page:
ENPP1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
ENPP1 Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. Note: This description may include information from UniProtKB.
Protein type: Phosphodiesterase; Motility/polarity/chemotaxis; Membrane protein, integral; Cofactor and Vitamin Metabolism - riboflavin; EC 3.6.1.9; EC 3.1.4.1; Nucleotide Metabolism - purine; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Carbohydrate Metabolism - starch and sucrose
Chromosomal Location of Human Ortholog: 6q22-q23
Cellular Component: cell surface; extracellular space; integral to membrane; integral to plasma membrane; lysosomal membrane; plasma membrane
Molecular Function: 3'-phosphoadenosine 5'-phosphosulfate binding; ATP binding; calcium ion binding; insulin receptor binding; nucleoside-triphosphate diphosphatase activity; nucleotide diphosphatase activity; phosphodiesterase I activity; protein binding; protein homodimerization activity; zinc ion binding
Biological Process: 3'-phosphoadenosine 5'-phosphosulfate metabolic process; cellular phosphate ion homeostasis; cellular response to insulin stimulus; generation of precursor metabolites and energy; inorganic diphosphate transport; negative regulation of cell growth; negative regulation of fat cell differentiation; negative regulation of glucose import; negative regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; negative regulation of protein amino acid autophosphorylation; nucleoside triphosphate catabolic process; phosphate metabolic process; regulation of bone mineralization; riboflavin metabolic process; sequestering of triacylglycerol
Disease: Arterial Calcification, Generalized, Of Infancy, 1; Cole Disease; Diabetes Mellitus, Noninsulin-dependent; Hypophosphatemic Rickets, Autosomal Recessive, 2; Obesity
Reference #:  P22413 (UniProtKB)
Alt. Names/Synonyms: alkaline phosphodiesterase 1; Alkaline phosphodiesterase I; ARHR2; E-NPP 1; ectonucleotide pyrophosphatase/phosphodiesterase 1; Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; ENPP1; Ly-41 antigen; M6S1; Membrane component chromosome 6 surface marker 1; membrane component, chromosome 6, surface marker 1; NPP1; NPPase; NPPS; Nucleotide pyrophosphatase; PC-1; PC1; PCA1; PDNP1; Phosphodiesterase I/nucleotide pyrophosphatase 1; plasma-cell membrane glycoprotein 1; Plasma-cell membrane glycoprotein PC-1
Gene Symbols: ENPP1
Molecular weight: 104,924 Da
Basal Isoelectric point: 6.76  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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ENPP1

Protein Structure Not Found.
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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S11‑p DGCAGGGsRGGEGGR
0 4 P43 AAEAPGDPQAAASLL
0 1 K62‑ac VGEEPLEkAArARTA
0 1 R65‑m1 EPLEkAArARTAKDP
0 1 T74 RTAKDPNTYKVLSLV
0 1 Y75 TAKDPNTYKVLSLVL
0 1 T88 VLSVCVLTTILGCIF
0 1 T89 LSVCVLTTILGCIFG
0 1 K255 MRPVYPTKtFPNHYS
1 7 T256‑p RPVYPTKtFPNHYSI
0 1 K278 SHGIIDNKMyDPKMN
0 1 Y280‑p GIIDNKMyDPKMNAS
0 1 K295 FSLKSKEKFNPEWYK
  mouse

 
- gap
S25‑p GNGRELEsPAAASLL
K44 LGEEPLEKAERARPA
R47 EPLEKAERARPAKDP
T56 RPAKDPNTYKVLSLV
Y57 PAKDPNTYKVLSLVL
T70 VLSVCVLTTILGCIF
T71 LSVCVLTTILGCIFG
K237 MRPMYPTKtFPNHYS
T238‑p RPMYPTKtFPNHYSI
K260 SHGIIDNKMYDPKMN
Y262 GIIDNKMYDPKMNAS
K277 FSLKSKEKFNPLWYK
  rat

 
- gap
S25‑p GNGRELEsPAAASLL
K44 LGEEPLEKAERARTA
R47 EPLEKAERARTAKDP
T56‑p RTAKDPNtyKVLSLV
Y57‑p TAKDPNtyKVLSLVL
T70‑p VLSVCVLttILGCIF
T71‑p LSVCVLttILGCIFG
K237‑ac MRPVYPTktFPNHYS
T238‑p RPVYPTktFPNHYSI
K260‑ac SHGIIDNkMYDPKMN
Y262 GIIDNkMYDPKMNAS
K277‑ac FSLKSKEkFNPLWYK
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