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Protein Page:
GRK2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
GRK2 a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.15; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; AGC group; GRK family; BARK subfamily
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: cytoplasm; cytosol; membrane; plasma membrane
Molecular Function: alpha-2A adrenergic receptor binding; ATP binding; beta-adrenergic receptor kinase activity; Edg-2 lysophosphatidic acid receptor binding; G-protein coupled receptor kinase activity; protein binding; protein kinase activity
Biological Process: acetylcholine receptor signaling, muscarinic pathway; cardiac muscle contraction; desensitization of G-protein coupled receptor protein signaling pathway; entry of virus into host cell; heart development; negative regulation of striated muscle contraction; negative regulation of the force of heart contraction by chemical signal; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of catecholamine secretion; receptor internalization; tachykinin signaling pathway; viral genome replication
Reference #:  P25098 (UniProtKB)
Gene Symbols: ADRBK1
Molecular weight: 79,574 Da
Basal Isoelectric point: 6.89  Predict pI for various phosphorylation states
CST Pathways:  GPCR Signaling to MAPKs
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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GRK2

Protein Structure Not Found.
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Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
cell cycle regulation: S670‑p
cell growth, altered: S670‑p
activity, induced: S29‑p
molecular association, regulation: Y13‑p, Y86‑p, Y92‑p, S670‑p, S685‑p
protein degradation: Y13‑p, Y86‑p, Y92‑p, S670‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
5 0 Y13‑p AVLADVSyLMAMEKS
2 0 S29‑p ATPAARAsKKILLPE
0 1 T54‑p LEDRGEVtFEkIFSQ
0 1 K57‑ub RGEVtFEkIFSQkLG
0 4 K62‑ub FEkIFSQkLGYLLFR
5 0 Y86‑p ARPLVEFyEEIKKyE
5 0 Y92‑p FyEEIKKyEKLETEE
0 1 K126‑ub ACSHPFSkSATEHVQ
0 1 K170‑ub QKFIESDkFTRFCQW
0 1 S247‑p RIMLSLVstGDCPFI
0 1 T248‑p IMLSLVstGDCPFIV
0 1 T263‑p CMSYAFHtPDKLSFI
0 1 K319‑ub FVVYRDLkPANILLD
0 4 K344‑ub GLACDFSkkKPHASV
0 1 K345‑ub LACDFSkkKPHASVG
0 71 Y356‑p ASVGTHGyMAPEVLQ
0 1 S416‑p MAVELPDsFSPELRS
0 1 K448‑ub GRGAQEVkESPFFRS
0 4 S487‑p ADAFDIGsFDEEDTK
0 1 S501‑p KGIKLLDsDQELyRN
0 1 Y506‑p LDsDQELyRNFPLTI
0 1 K628‑ub LLKIRGGkQFILQCD
0 1 K644‑ac DPELVQWkKELRDAY
3 46 S670‑p KMKNKPRsPVVELsk
0 2 S676‑p RsPVVELskVPLVQR
0 2 K677‑ub sPVVELskVPLVQRG
2 9 S685‑p VPLVQRGsANGL___
  mouse

 
Y13‑p AVLADVSyLMAMEKS
S29 ATPAARASKKILLPE
T54 LEDRGEVTFEKIFSQ
K57 RGEVTFEKIFSQKLG
K62 FEKIFSQKLGYLLFR
Y86‑p AKPLVEFyEEIKKyE
Y92‑p FyEEIKKyEKLETEE
K126 ACSHPFSKNATEHVQ
K170 QKFIESDKFTRFCQW
S247 RIMLSLVSTGDCPFI
T248 IMLSLVSTGDCPFIV
T263 CMSYAFHTPDKLSFI
K319 FVVYRDLKPANILLD
K344‑ub GLACDFSkKRPHASV
K345 LACDFSkKRPHASVG
Y356‑p ASVGTHGyMAPEVLQ
S416 MAVELPDSFSPELRS
K448 GRGAQEVKESPFFRS
S487‑p ADAFDIGsFDEEDTK
S501 KGIKLLDSDQELYRN
Y506 LDSDQELYRNFPLTI
K628 LLKIRGGKQFVLQCD
K644 DPELVQWKKELRDAY
S670‑p KMKNKPRsPVVELsK
S676‑p RsPVVELsKVPLIQR
K677 sPVVELsKVPLIQRG
S685 VPLIQRGSANGL___
  rat

 
Y13 AVLADVSYLMAMEKS
S29‑p ATPAARAsKKILLPE
T54 LEDRGEVTFEKIFSQ
K57 RGEVTFEKIFSQKLG
K62 FEKIFSQKLGYLLFR
Y86 AKPLVEFYEEIEKYE
Y92 FYEEIEKYEKLETEE
K126 ACSHPFSKNATEHVQ
K170 HKFIESDKFTRFCQW
S247 RIMLSLVSTGDCPFI
T248 IMLSLVSTGDCPFIV
T263 CMSYAFHTPDKLSFI
K319 FVVYRDLKPANILLD
K344 GLACDFSKKKPHASV
K345 LACDFSKKKPHASVG
Y356 ASVGTHGYMAPEVLQ
S416 MAVELPDSFSPELRS
K448 GRGAQEIKESPFFRS
S487 ADAFDIGSFDEEDTK
S501 KGIKLLDSDQELYRN
Y506 LDSDQELYRNFPLTI
K628 LLKIRGGKQFVLQCD
K644 DPELVQWKKELRDAY
S670‑p KMKNKPRsPVVELSK
S676 RsPVVELSKVPLIQR
K677 sPVVELSKVPLIQRG
S685 VPLIQRGSANGL___
  cow

 
Y13‑p AVLADVSyLMAMEKS
S29 ATPAARASKKILLPE
T54 LEDRGEVTFEKIFSQ
K57 RGEVTFEKIFSQKLG
K62 FEKIFSQKLGYLLFR
Y86‑p AKPLVEFyEEIKKyE
Y92‑p FyEEIKKyEKLETEE
K126 ACSHPFSKSAIEHVQ
K170 QKFIESDKFTRFCQW
S247 RIMLSLVSTGDCPFI
T248 IMLSLVSTGDCPFIV
T263 CMSYAFHTPDKLSFI
K319 FVVYRDLKPANILLD
K344 GLACDFSKKKPHASV
K345 LACDFSKKKPHASVG
Y356 ASVGTHGYMAPEVLQ
S416 MAVELPDSFSPELRS
K448 GRGAQEVKESPFFRS
S487 ADAFDIGSFDEEDTK
S501 KGIKLLDSDQELYRN
Y506 LDSDQELYRNFPLTI
K628 LLKIRGGKQFVLQCD
K644 DPELVQWKKELRDAY
S670‑p KMKNKPRsPVVELSK
S676 RsPVVELSKVPLIQR
K677 sPVVELSKVPLIQRG
S685‑p VPLIQRGsANGL___
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