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Protein Page:
DAPK1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
DAPK1 a CAMK protein kinase of the Death-associated protein kinase (DAPK) family. Mediates autophagy and is a positive mediator of ER stress-induced and gamma-interferon induced programmed cell death. Catalytic activity is increased by ER stress. Inhibited by autophosphorylation of S308. DAPK -/- cells are protected from ER stress-induced cell death, and both apoptosis and autophagy are decreased. Colocalizes with the actin filament system. It is a tumor suppressor candidate, activating p53-dependent apoptosis. Expression is reduced in some cancers, and the promoter is frequently hypermethylated in invasive cancers. Increased expression and mislocalization is seen in epilepsy, where it may regulate neuronal death. A structurally unique serine-threonine kinase that carries eight ankyrin repeats and two putative P-loop consensus sites. Two isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.1; Autophagy; Apoptosis; Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; DAPK family
Chromosomal Location of Human Ortholog: 9q21.33
Cellular Component: actin cytoskeleton; cytoplasm; plasma membrane
Molecular Function: ATP binding; calmodulin binding; calmodulin-dependent protein kinase activity; GTP binding; identical protein binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; syntaxin-1 binding
Biological Process: apoptosis; induction of apoptosis via death domain receptors; negative regulation of translation; positive regulation of caspase activity; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of autophagy
Reference #:  P53355 (UniProtKB)
Alt. Names/Synonyms: DAP kinase 1; DAPK; DAPK1; Death-associated protein kinase 1; DKFZp781I035
Gene Symbols: DAPK1
Molecular weight: 160,046 Da
Basal Isoelectric point: 6.37  Predict pI for various phosphorylation states
CST Pathways:  Death Receptor Signaling  |  Growth And Differentiation Control by MAPKs
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

DAPK1

Protein Structure Not Found.
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Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
apoptosis, altered: S289‑p, S308‑p, S734‑p
apoptosis, induced: S734‑p
cell adhesion, altered: S308‑p
cell adhesion, inhibited: S734‑p
cell growth, altered: S308‑p
activity, inhibited: S289‑p
enzymatic activity, induced: S734‑p
enzymatic activity, inhibited: S308‑p
molecular association, regulation: S308‑p
protein degradation: S308‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 T15‑p NVDDYYDtGEELGSG
0 1 Y39‑p EKSTGLQyAAKFIKK
0 1 S57‑p KSSRRGVsREDIERE
0 1 T112‑p LAEKESLtEEEATEF
1 30 S289‑p QALSRKAsAVNMEkF
0 3 K295‑ac AsAVNMEkFkKFAAR
0 3 K297‑ac AVNMEkFkKFAARKK
7 3 S308‑p ARKKWKQsVRLISLC
0 6 S319‑p ISLCQRLsRsFLsRs
0 3 S321‑p LCQRLsRsFLsRsNM
0 2 S324‑p RLsRsFLsRsNMSVA
0 4 S326‑p sRsFLsRsNMSVARs
0 9 S333‑p sNMSVARsDDTLDEE
0 3 T336 SVARsDDTLDEEDSF
0 1 S368 QHLLGSLSNYDVNQP
0 1 Y455 ALHVAARYGHADVAQ
0 1 S469 QLLCSFGSNPNIQDK
1 0 Y490‑p HCAAWHGyySVAKAL
1 0 Y491‑p CAAWHGyySVAKALC
0 1 S606‑p ANCNLDIsNKYGRTP
0 1 T701 GHSGSGKTTLVESLK
0 1 S706 GKTTLVESLKCGLLR
0 1 K708 TTLVESLKCGLLRSF
0 1 S724‑p RRRRPRLssTNsSRF
0 1 S725‑p RRRPRLssTNsSRFP
0 1 S728‑p PRLssTNsSRFPPsP
2 7 S734‑p NsSRFPPsPLASKPT
0 3 S762‑p NVSVRSRsMMFEPGL
0 1 K875 EPIAFGGKLKNPLQV
0 1 K912 DKDTSLLKEIRNRFG
0 2 K939‑ub DAGASGSkDMKVLRN
0 1 K942 ASGSkDMKVLRNHLQ
0 1 Y1060‑p TPRALHHyRGRYTVE
0 1 K1108 VDVPALIKTDNLHRS
0 2 S1115 KTDNLHRSWADEEDE
0 1 T1266‑p RAQTLKEtsLtNTMG
0 1 S1267‑p AQTLKEtsLtNTMGG
0 1 T1269‑p TLKEtsLtNTMGGYK
0 3 - gap
  mouse

 
T15 NVDDYYDTGEELGSG
Y39 EKSTGLQYAAKFIKK
S57 KSSRRGVSREDIERE
T112 LAEKESLTEEEATEF
S289‑p QALSRKAsAVNMEKF
K295 AsAVNMEKFKKFAAR
K297 AVNMEKFKKFAARKK
S308‑p ARKKWKQsVRLISLC
S319‑p ISLCQRLsRsFLsRs
S321‑p LCQRLsRsFLsRsNM
S324‑p RLsRsFLsRsNMSVA
S326‑p sRsFLsRsNMSVARs
S333‑p sNMSVARsDDtLDEE
T336‑p SVARsDDtLDEEDSF
S368‑p QHLLGSLsSYDVNQP
Y455‑p ALHVAARyGHADVVQ
S469‑p QLLCSFGsNPDFQDK
Y490 HCAAWHGYYSVAKAL
Y491 CAAWHGYYSVAKALC
S606 ASCNLDISNKYGRTP
S701‑p GHSGSGKsTLVEsLk
S706‑p GKsTLVEsLkCGLLR
K708‑ub sTLVEsLkCGLLRSF
S724 RRRRPRLSSTNSTRF
S725 RRRPRLSSTNSTRFP
S728 PRLSSTNSTRFPPsP
S734‑p NSTRFPPsPLAAKPT
S762‑p NVSVRSRsMMFEPGL
K875‑ub EPIAFGGkLKNPLRV
K912‑ub DKDTSLLkEIRNRFG
K939‑ub DAGASGSkDIkVLRN
K942‑ub ASGSkDIkVLRNHLQ
Y1060 TPRALHHYRGRYTME
K1108‑ub VDIPALIkTDSLQRs
S1115‑p kTDSLQRsWADEEDE
S1266 RAQTLKESSLTNTMG
S1267 AQTLKESSLTNTMGG
T1269 TLKESSLTNTMGGYK
S1433‑p SVVSRRDsHAWTPLY
  rat

 
T15 NVDDYYDTGEELGSG
Y39 EKSTGLQYAAKFIKK
S57 KSSRRGVSREDIERE
T112 LAEKESLTEEEATEF
S289 QALSRKASAVNMEKF
K295 ASAVNMEKFKKFAAR
K297 AVNMEKFKKFAARKK
S308 ARKKWKQSVRLISLC
S319 ISLCQRLSRSFLSRS
S321 LCQRLSRSFLSRSNM
S324 RLSRSFLSRSNMSVA
S326 SRSFLSRSNMSVARS
S333 SNMSVARSDDTLDEE
T336 SVARSDDTLDEEDSF
S368 QHLLGSLSSYDVNQP
Y455 ALHVAARYGHADVVQ
S469 QLLCSFGSNPDFQDK
Y490 HCAAWHGYYSVARAL
Y491 CAAWHGYYSVARALC
S606 ASCNLDISNKYGRTP
S701 GHSGSGKSTLVESLK
S706 GKSTLVESLKCGLLR
K708 STLVESLKCGLLRSF
S724 RRRRPRLSSTNSTRF
S725 RRRPRLSSTNSTRFP
S728 PRLSSTNSTRFPPsP
S734‑p NSTRFPPsPLATKPT
S762 NVSVRSRSMMFEPGL
K875 EPIAFGGKLKNPLRV
K912 DKDTSLLKEIRNRFG
K939 DAGASGSKDIKVLRN
K942 ASGSKDIKVLRNHLQ
Y1060 TPRALHHYRGRYTME
K1108 VDIPALIKTDSLQRS
S1115 KTDSLQRSWADEEDE
S1266 RAQTLKESSLTNTMG
S1267 AQTLKESSLTNTMGG
T1269 TLKESSLTNTMGGYK
- gap
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