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Protein Page:
CDK4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
CDK4 a protein kinase of the CDK family that is important for cell cycle G1 phase progression. Its activity is restricted to the G1-S phase. Controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). Phosphorylates the retinoblastoma gene product (Rb). Point mutations found in somatic and familial melanoma. Amplified in sarcomas, glioma and lymphoma. Amplified, methylated or deleted in head and neck squamous cell carcinoma. Overexpression drives epithelial tumors in mice. Disruption makes mice resistant to cancer. Inhibitor: PD332991. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, CMGC; Kinase, protein; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; CMGC group; CDK family; CDK4 subfamily; CDK/CDK4 subfamily
Chromosomal Location of Human Ortholog: 12q14
Cellular Component: chromatin; cyclin-dependent protein kinase holoenzyme complex; cytosol; nuclear membrane; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm; tight junction; transcription factor complex
Molecular Function: ATP binding; cyclin binding; cyclin-dependent protein kinase activity; cyclin-dependent protein kinase regulator activity; protein binding; protein complex binding
Biological Process: cell division; circadian rhythm; G1/S transition of mitotic cell cycle; lens development in camera-type eye; organ regeneration; positive regulation of apoptosis; positive regulation of cell cycle; positive regulation of cell proliferation; positive regulation of cell size; positive regulation of fibroblast proliferation; positive regulation of translation; protein amino acid phosphorylation; regulation of gene expression; regulation of protein kinase activity; response to drug; response to hyperoxia; response to lead ion; response to testosterone stimulus; response to toxin; signal transduction
Disease: Melanoma, Cutaneous Malignant, Susceptibility To, 3
Reference #:  P11802 (UniProtKB)
Alt. Names/Synonyms: CDK4; cell division kinase 4; Cell division protein kinase 4; CMM3; Cyclin-dependent kinase 4; melanoma cutaneous malignant, 3; MGC14458; PSK-J3
Gene Symbols: CDK4
Molecular weight: 33,730 Da
Basal Isoelectric point: 6.52  Predict pI for various phosphorylation states
CST Pathways:  G1/S Checkpoint
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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CDK4

Protein Structure Not Found.
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Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
enzymatic activity, induced: T172‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 3 Y17‑p AEIGVGAyGTVYkAR
0 2 K22‑ub GAyGTVYkARDPHSG
0 1 K35 SGHFVALKSVRVPNG
0 1 K35‑ub SGHFVALkSVRVPNG
0 2 K106‑ub DLRTYLDkAPPPGLP
0 1 K118‑ub GLPAETIkDLMRQFL
0 1 K142‑ub CIVHRDLkPENILVT
0 1 K155‑ub VTSGGTVkLADFGLA
6 5 T172‑p YSYQMALtPVVVTLW
0 1 K211‑ub FAEMFRRkPLFCGNS
0 1 P250 SLPRGAFPPRGPRPV
0 1 K297‑ub LQHSYLHkDEGNPE_
0 17 G300 SYLHkDEGNPE____
  mouse

 
Y17 AEIGVGAYGTVYKAR
K22 GAYGTVYKARDPHSG
K35 SGHFVALKSVRVPNG
K35 SGHFVALKSVRVPNG
K106 DLRTYLDKAPPPGLP
K118 GLPVETIKDLMRQFL
K142 CIVHRDLKPENILVT
K155 VTSNGTVKLADFGLA
T172‑p YSYQMALtPVVVTLW
K211 FAEMFRRKPLFCGNS
A250 SLPRGAFAPRGPRPV
K297 LQHSYLHKEEsDAE_
S300‑p SYLHKEEsDAE____
  rat

 
Y17 AEIGVGAYGTVYKAR
K22 GAYGTVYKARDPHSG
K35‑ac SGHFVALkSVRVPNG
K35 SGHFVALKSVRVPNG
K106 DLRTYLDKAPPPGLP
K118 GLPVETIKDLMRQFL
K142 CIVHRDLKPENILVT
K155 VTSNGTVKLADFGLA
T172 YSYQMALTPVVVTLW
K211 FAEMFRRKPLFCGNS
S250‑p SLPRGAFsPRGPRPV
K297 LQHSYLHKEEsDPE_
S300‑p SYLHKEEsDPE____
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