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Protein Page:
TCF8 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
TCF8 Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. Interacts (via N-terminus) with SMARCA4/BRG1. Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas. Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family. Note: This description may include information from UniProtKB.
Protein type: C2H2-type zinc finger protein; DNA-binding; Motility/polarity/chemotaxis; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 10p11.2
Cellular Component: cytoplasm; nucleoplasm; nucleus
Molecular Function: protein binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity; zinc ion binding
Biological Process: cell proliferation; immune response; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of neuron differentiation; regulation of transcription from RNA polymerase II promoter
Disease: Corneal Dystrophy, Fuchs Endothelial, 6; Corneal Dystrophy, Posterior Polymorphous, 3
Reference #:  P37275 (UniProtKB)
Alt. Names/Synonyms: AREB6; BZP; delta-crystallin enhancer binding factor 1; DELTAEF1; FECD6; MGC133261; Negative regulator of IL2; NIL-2-A zinc finger protein; NIL2A; TCF-8; TCF8; Transcription factor 8; transcription factor 8 (represses interleukin 2 expression); ZEB1; ZFHEP; ZFHX1A; zinc finger E-box binding homeobox 1; Zinc finger E-box-binding homeobox 1; zinc finger homeodomain enhancer-binding protein
Gene Symbols: ZEB1
Molecular weight: 124,074 Da
Basal Isoelectric point: 4.87  Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics  |  Wnt/ß-Catenin Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

TCF8

Protein Structure Not Found.
Download PyMol Script
Download ChimeraX Script


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 8 S31‑p NTVVETNsDsDDEDK
0 9 S33‑p VVETNsDsDDEDKLH
0 2 T151‑p EEDQRQGtPEAsGHD
0 1 S155‑p RQGtPEAsGHDENGt
0 2 T162‑p sGHDENGtPDAFSQL
0 1 S184‑p RGYKRFTsLKEHIKY
0 5 S263‑p KEHLRIHsGEKPYEC
0 2 S282‑p KRFSHSGsYSSHISS
0 3 S309‑p PRTGLKTsQCssPsL
0 2 S312‑p GLKTsQCssPsLsAs
0 9 S313‑p LKTsQCssPsLsAsP
0 6 S315‑p TsQCssPsLsAsPGs
0 3 S317‑p QCssPsLsAsPGsPt
0 8 S319‑p ssPsLsAsPGsPtRP
0 28 S322‑p sLsAsPGsPtRPQIR
0 5 T324‑p sAsPGsPtRPQIRQK
0 1 S342‑p KPLQEQLsVNQIKTE
0 1 T443‑p LASKEQEtINAsPIQ
0 3 S447‑p EQEtINAsPIQQGGH
0 1 S455‑p PIQQGGHsVISAISL
0 1 K493‑sm QVVPQNLkKENPVAT
0 1 K504‑ub PVATNSCkSEKLPED
0 1 K504‑sm PVATNSCkSEKLPED
0 1 K507 TNSCkSEKLPEDLTV
0 1 K515 LPEDLTVKsEKDKsF
0 1 K515‑sm LPEDLTVksEKDKsF
0 2 S516‑p PEDLTVksEKDKsFE
0 2 S521‑p VksEKDKsFEGGVND
0 1 S529‑p FEGGVNDsTCLLCDD
0 4 S583‑p ATGDGNLsPsQPPLK
1 0 S585‑p GDGNLsPsQPPLKNL
0 4 S641‑p AGQISVQssEPssPE
0 3 S642‑p GQISVQssEPssPEP
0 2 S645‑p SVQssEPssPEPGKV
0 11 S646‑p VQssEPssPEPGKVN
0 15 S679‑p DSTVNLQsPLKMTNs
0 1 T684 LQsPLKMTNsPVLPV
0 2 N685 QsPLKMTNsPVLPVG
0 4 S686‑p sPLKMTNsPVLPVGS
0 2 S693 sPVLPVGSTTNGSRs
0 12 S700‑p STTNGSRsstPsPsP
0 4 S701‑p TTNGSRsstPsPsPL
0 8 T702‑p TNGSRsstPsPsPLN
0 4 P703 NGSRsstPsPsPLNL
0 16 S704‑p GSRsstPsPsPLNLS
0 4 S706‑p RsstPsPsPLNLSSS
0 1 S711 sPsPLNLSSSRNtQG
0 1 T716‑p NLSSSRNtQGYLYTA
0 1 S850‑p YTYSTTVsPAVQEPP
0 14 S887‑p NVEDQNDsDstPPKK
0 3 S889‑p EDQNDsDstPPKKKM
1 5 T890‑p DQNDsDstPPKKKMR
0 1 T927‑p LRHKYEHtGKRPHEC
0 2 S995‑p REAEERDstEQEEAG
0 1 T996‑p EAEERDstEQEEAGP
0 1 S1016 EHVGARASPsQGDsD
0 1 S1018‑p VGARASPsQGDsDER
0 2 S1022‑p ASPsQGDsDERESLT
0 3 S1036 TREEDEDSEKEEEEE
0 4 S1100‑p MKDDRAEsQASSLGQ
  TCF8 iso2  
S31 NTVVETNSDSDDEDK
S33 VVETNSDSDDEDKLH
T152 EEDQRQGTPEASGHD
S156 RQGTPEASGHDENGT
T163 SGHDENGTPDAFSQL
S185 RGYKRFTSLKEHIKY
S264 KEHLRIHSGEKPYEC
S283 KRFSHSGSYSSHISS
S310 PRTGLKTSQCSSPSL
S313 GLKTSQCSSPSLSAS
S314 LKTSQCSSPSLSASP
S316 TSQCSSPSLSASPGS
S318 QCSSPSLSASPGSPT
S320 SSPSLSASPGSPTRP
S323 SLSASPGSPTRPQIR
T325 SASPGSPTRPQIRQK
S343 KPLQEQLSVNQIKTE
T444 LASKEQETINASPIQ
S448 EQETINASPIQQGGH
S456 PIQQGGHSVISAISL
K494 QVVPQNLKKENPVAT
K505 PVATNSCKSEKLPED
K505 PVATNSCKSEKLPED
K508 TNSCKSEKLPEDLTV
K516 LPEDLTVKSEKDKSF
K516 LPEDLTVKSEKDKSF
S517 PEDLTVKSEKDKSFE
S522 VKSEKDKSFEGGVND
S530 FEGGVNDSTCLLCDD
S584 ATGDGNLSPSQPPLK
S586 GDGNLSPSQPPLKNL
S642 AGQISVQSSEPSSPE
S643 GQISVQSSEPSSPEP
S646 SVQSSEPSSPEPGKV
S647 VQSSEPSSPEPGKVN
S680 DSTVNLQSPLKMTNS
T685 LQSPLKMTNSPVLPV
N686 QSPLKMTNSPVLPVG
S687 SPLKMTNSPVLPVGS
S694 SPVLPVGSTTNGSRS
S701 STTNGSRSSTPSPSP
S702 TTNGSRSSTPSPSPL
T703 TNGSRSSTPSPSPLN
P704 NGSRSSTPSPSPLNL
S705 GSRSSTPSPSPLNLS
S707 RSSTPSPSPLNLSSS
S712 SPSPLNLSSSRNTQG
T717 NLSSSRNTQGYLYTA
S851 YTYSTTVSPAVQEPP
S888 NVEDQNDSDSTPPKK
S890 EDQNDSDSTPPKKKM
T891 DQNDSDSTPPKKKMR
T928 LRHKYEHTGKRPHEC
S996 REAEERDSTEQEEAG
T997 EAEERDSTEQEEAGP
S1017 EHVGARASPSQGDSD
S1019 VGARASPSQGDSDER
S1023 ASPSQGDSDERESLT
S1037 TREEDEDSEKEEEEE
S1101 MKDDRAESQASSLGQ
  mouse

 
S31‑p NTVVEANsDsDDEDK
S33‑p VVEANsDsDDEDKLH
T131 EEDQRQGTPEASSHD
S135 RQGTPEASSHDENGT
T142 SSHDENGTPDAFSQL
S164 RGYKRFTSLKEHIKY
S243 KEHLRIHSGEKPYEC
S262 KRFSHSGSYSSHISS
S289 PRSGLKTSQCSsPSL
S292 GLKTSQCSsPSLSTs
S293‑p LKTSQCSsPSLSTsP
S295 TSQCSsPSLSTsPGs
S297 QCSsPSLSTsPGsPt
S299‑p SsPSLSTsPGsPtRP
S302‑p SLSTsPGsPtRPQIR
T304‑p STsPGsPtRPQIRQK
S322 KPLQEPLSVNQIKTE
A423 LASKEQEAVSASPIQ
S427 EQEAVSASPIQQGGH
S435 PIQQGGHSVISAISL
K473 QVVPQNLKKEIPAPT
K484 PAPTNSCKSEKLPED
K484 PAPTNSCKSEKLPED
K487 TNSCKSEKLPEDLTV
K495 LPEDLTVKSETDKSF
K495 LPEDLTVKSETDKSF
S496 PEDLTVKSETDKSFE
S501 VKSETDKSFEGARDD
S509 FEGARDDSTCLLCED
S561 SAGNGDLSPSQPPLK
S563 GNGDLSPSQPPLKNL
S619‑p AGQIPGQsPDPPsPG
- gap
- gap
S624‑p GQsPDPPsPGTGSVN
S657‑p EDSTRGQsPVKIRss
R662 GQsPVKIRssPVLPV
S663‑p QsPVKIRssPVLPVG
S664‑p sPVKIRssPVLPVGs
S671‑p sPVLPVGsAMNGSRs
S678‑p sAMNGSRsCtssPsP
C679 AMNGSRsCtssPsPL
T680‑p MNGSRsCtssPsPLN
S681‑p NGSRsCtssPsPLNL
S682‑p GSRsCtssPsPLNLC
S684‑p RsCtssPsPLNLCSA
C689 sPsPLNLCSARNPQG
P694 NLCSARNPQGYSCVA
S828 YTYSATVSPAVQEPP
S865‑p TVEDQNDsDSTPPKK
S867 EDQNDsDSTPPKKKT
T868 DQNDsDSTPPKKKTR
T905 LRHKYEHTGKRPHEC
A973 RGAEDRDAMEQEDAG
M974 GAEDRDAMEQEDAGP
S998 EHVGARASPSQADSD
S1000 VGARASPSQADSDER
S1004 ASPSQADSDERESLT
S1018‑p TREEDEDsEKEEEEE
Q1086 TVEVGAAQQAGSLEQ
  rat

 
S31‑p NTVVEANsDsDDEDK
S33‑p VVEANsDsDDEDKLH
T131 EEDQRQGTPEASGHD
S135 RQGTPEASGHDDNGT
T142 SGHDDNGTPDAFSQL
S164 RGYKRFTSLKEHIKY
S243 KEHLRIHSGEKPYEC
S262 KRFSHSGSYSSHISS
S289 PRSGLKTSQCSSPSL
S292 GLKTSQCSSPSLSTS
S293 LKTSQCSSPSLSTSP
S295 TSQCSSPSLSTSPGS
S297 QCSSPSLSTSPGSPT
S299 SSPSLSTSPGSPTRP
S302 SLSTSPGSPTRPQIR
T304 STSPGSPTRPQIRQK
S322 KPLQEPLSVNQIKTE
A423 LASKEQEAVSASSIQ
S427 EQEAVSASSIQQGGH
S435 SIQQGGHSVISAISL
K473 QVVPQNLKKENPAPP
K484 PAPPKSCKSEkSPED
K484 PAPPKSCKSEkSPED
K487‑ac PKSCKSEkSPEDLTV
K495‑ac SPEDLTVkSEKDKSF
K495 SPEDLTVKSEKDKSF
S496 PEDLTVkSEKDKSFD
S501 VkSEKDKSFDGAADE
S509 FDGAADESTCLLCED
S561 SAGNGDLSPSQPPLK
S563 GNGDLSPSQPPLKNL
S619 AGQIPGQSLEPPsPG
- gap
- gap
S624‑p GQSLEPPsPGPGSGN
S657 EDSTRGQSPLKMtsS
T662‑p GQSPLKMtsSPVLPV
S663‑p QSPLKMtsSPVLPVG
S664 SPLKMtsSPVLPVGs
S671‑p SPVLPVGsAINGSRs
S678‑p sAINGSRsCTSsPSP
C679 AINGSRsCTSsPSPL
T680 INGSRsCTSsPSPLN
S681 NGSRsCTSsPSPLNL
S682‑p GSRsCTSsPSPLNLs
S684 RsCTSsPSPLNLsSA
S689‑p sPSPLNLsSARNPQG
P694 NLsSARNPQGYSCVS
S828 YTYSATVSPAMQEPP
S864 SVEDQNDSDCtPPKK
C866 EDQNDSDCtPPKKKT
T867‑p DQNDSDCtPPKKKTR
T904 LRHKYEHTGKRPHEC
A972 RGAEDRDAMEQEDTG
M973 GAEDRDAMEQEDTGP
S997‑p ELVGARAsPSQADsD
S999 VGARAsPSQADsDER
S1003‑p AsPSQADsDERESLT
S1017‑p TREEDEDsEKEEEEE
Q1084 AVEEEAAQQAGSFQQ
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