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Protein Page:
BRAF (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
BRAF a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.1; Kinase, protein; Oncoprotein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; RAF family
Chromosomal Location of Human Ortholog: 7q34
Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; plasma membrane
Molecular Function: calcium ion binding; identical protein binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; small GTPase binding
Biological Process: glucose transport; MAPKKK cascade; negative regulation of apoptosis; organ morphogenesis; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation
Disease: Cardiofaciocutaneous Syndrome 1; Leopard Syndrome 3; Lung Cancer; Noonan Syndrome 1; Noonan Syndrome 7
Reference #:  P15056 (UniProtKB)
Alt. Names/Synonyms: 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-RAF1; BRAF; BRAF1; FLJ95109; MGC126806; MGC138284; murine sarcoma viral (v-raf) oncogene homolog B1; p94; Proto-oncogene B-Raf; RAFB1; Serine/threonine-protein kinase B-raf; v-Raf murine sarcoma viral oncogene homolog B1
Gene Symbols: BRAF
Molecular weight: 84,437 Da
Basal Isoelectric point: 7.29  Predict pI for various phosphorylation states
CST Pathways:  ErbB/HER Signaling  |  ESC Pluripotency and Differentiation  |  GPCR Signaling to MAPKs  |  Growth And Differentiation Control by MAPKs  |  IL6 Signaling  |  Inhibition of Apoptosis  |  T Cell Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

BRAF

Protein Structure Not Found.
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Download ChimeraX Script


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Sites Implicated In
cell cycle regulation: S729‑p
cell differentiation, altered: T599‑p, S602‑p, S729‑p
cell growth, altered: S151‑p, S365‑p, T401‑p, S429‑p, T599‑p, S602‑p, S729‑p, S750‑p, T753‑p
cell growth, induced: S446‑p
cell growth, inhibited: S729‑p
transcription, altered: T599‑p, S602‑p
transcription, induced: S365‑p, S429‑p
enzymatic activity, induced: S446‑p, S579‑p, T599‑p, S602‑p, S729‑p
enzymatic activity, inhibited: S365‑p, T401‑p, S429‑p, T440‑p
molecular association, regulation: S151‑p, T401‑p, S446‑p, T599‑p, S602‑p, S729‑p, S750‑p, T753‑p
protein conformation: S446‑p, T599‑p, S602‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 0 - gap
1 0 S76‑p GGEHNPPsIYLEAYE
1 0 T119‑p SSSASMDtVTSSSSS
0 1 S147‑p NPTDVARsNPKsPQK
3 49 S151‑p VARsNPKsPQKPIVR
1 0 K154 sNPKsPQKPIVRVFL
0 9 T244‑p FVRKTFFtLAFCDFC
0 1 K253‑ac AFCDFCRkLLFQGFR
1 0 S314‑p LAETALTsGssPsAP
1 0 S316‑p ETALTsGssPsAPAS
1 0 S317‑p TALTsGssPsAPASD
1 1 S319‑p LTsGssPsAPASDSI
1 4 S333‑p IGPQILTsPsPSKsI
1 4 S335‑p PQILTsPsPSKsIPI
1 0 S337 ILTsPsPSKsIPIPQ
1 0 S339‑p TsPsPSKsIPIPQPF
2 33 S363‑p QFGQRDRsssAPNVH
1 9 S364‑p FGQRDRsssAPNVHI
8 50 S365‑p GQRDRsssAPNVHIN
2 6 T373‑p APNVHINtIEPVNID
1 1 S394‑p GFRGDGGsTtGLsAt
1 1 T396‑p RGDGGsTtGLsAtPP
1 2 S399‑p GGsTtGLsAtPPAsL
3 28 T401‑p sTtGLsAtPPAsLPG
0 1 S405‑p LsAtPPAsLPGSLTN
0 1 S409 PPAsLPGSLTNVKAL
0 1 K418‑ac TNVKALQksPGPQRE
2 38 S419‑p NVKALQksPGPQRER
1 8 S428‑p GPQRERKsssssEDR
7 33 S429‑p PQRERKsssssEDRN
1 6 S430‑p QRERKsssssEDRNR
1 3 S431‑p RERKsssssEDRNRM
1 5 S432‑p ERKsssssEDRNRMK
2 6 T440‑p EDRNRMKtLGRRDss
13 122 S446‑p KtLGRRDssDDWEIP
1 149 S447‑p tLGRRDssDDWEIPD
0 1 T458‑p EIPDGQItVGQRIGs
2 8 S465‑p tVGQRIGsGsFGtVY
1 0 S467‑p GQRIGsGsFGtVYKG
1 1 T470‑p IGsGsFGtVYKGKWH
1 0 K578‑ub SIIHRDLksNNIFLH
1 0 S579‑p IIHRDLksNNIFLHE
10 0 T599‑p IGDFGLAtVksRWsG
0 2 K601‑ub DFGLAtVksRWsGSH
10 0 S602‑p FGLAtVksRWsGSHQ
1 2 S605‑p AtVksRWsGSHQFEQ
2 1 S614‑p SHQFEQLsGSILWMA
1 0 S616 QFEQLsGSILWMAPE
1 0 S675‑p MVGRGYLsPDLSKVR
0 1 K687‑ub KVRSNCPkAMKRLMA
6 71 S729‑p PKIHRSAsEPsLNRA
0 3 S732‑p HRSAsEPsLNRAGFQ
0 1 Y746‑p QTEDFSLyACAsPKt
2 9 S750‑p FSLyACAsPKtPIQA
4 5 T753‑p yACAsPKtPIQAGGy
0 1 Y760‑p tPIQAGGyGAFPVH_
2696 : Phospho-B-Raf (Ser445) Antibody
  mouse

► Hide Isoforms
 
- gap
S93 GGEHNPPSIYLEAYE
- gap
N131 TPTDASRNNPKsPQk
S135‑p ASRNNPKsPQkPIVR
K138‑ub NNPKsPQkPIVRVFL
T228 FVRKTFFTLAFCDFC
K237 AFCDFCRKLLFQGFR
S298‑p FPETALPsGssSAPP
S300‑p ETALPsGssSAPPSD
S301‑p TALPsGssSAPPSDS
S302 ALPsGssSAPPSDST
S316‑p TGPQILTsPsPSKSI
S318‑p PQILTsPsPSKSIPI
S320 ILTsPsPSKSIPIPQ
S322 TsPsPSKSIPIPQPF
S346‑p QFGQRDRsSsAPNVH
S347 FGQRDRsSsAPNVHI
S348‑p GQRDRsSsAPNVHIN
T356 APNVHINTIEPVNID
S429 PGPVFRGSTTGLsAt
T431 PVFRGSTTGLsAtPP
S434‑p RGSTTGLsAtPPASL
T436‑p STTGLsAtPPASLPG
S440 LsAtPPASLPGSLTN
S444 PPASLPGSLTNVKAL
K453 TNVKALQKsPGPQRE
S454‑p NVKALQKsPGPQRER
S463‑p GPQRERKsssssSSE
S464‑p PQRERKsssssSSED
S465‑p QRERKsssssSSEDR
S466‑p RERKsssssSSEDRS
S467‑p ERKsssssSSEDRSR
T477 EDRSRMKTLGRRDss
S483‑p KTLGRRDssDDWEIP
S484‑p TLGRRDssDDWEIPD
T495 EIPDGQITVGQRIGs
S502‑p TVGQRIGsGSFGTVY
S504 GQRIGsGSFGTVYKG
T507 IGsGSFGTVYKGKWH
K615 SIIHRDLKSNNIFLH
S616 IIHRDLKSNNIFLHE
T636 IGDFGLATVKSRWsG
K638 DFGLATVKSRWsGSH
S639 FGLATVKSRWsGSHQ
S642‑p ATVKSRWsGSHQFEQ
S651‑p SHQFEQLsGsILWMA
S653‑p QFEQLsGsILWMAPE
S712‑p MVGRGYLsPDLSKVR
K724 KVRSNCPKAMKRLMA
S766‑p PKIHRSAsEPsLNRA
S769‑p HRSAsEPsLNRAGFQ
Y783 QTEDFSLYACAsPKt
S787‑p FSLYACAsPKtPIQA
T790‑p YACAsPKtPIQAGGY
Y797 tPIQAGGYGEFAAFK
2696 : Phospho-B-Raf (Ser445) Antibody
  BRAF iso3  
- gap
S10 GGEHNPPSIYLEAYE
T53 SSSASMDTVTSSSSS
N81 TPTDASRNNPKSPQK
S85 ASRNNPKSPQKPIVR
K88 NNPKSPQKPIVRVFL
T178 FVRKTFFTLAFCDFC
K187 AFCDFCRKLLFQGFR
S248 FPETALPSGSSSAPP
S250 ETALPSGSSSAPPSD
S251 TALPSGSSSAPPSDS
S252 ALPSGSSSAPPSDST
S266 TGPQILTSPSPSKSI
S268 PQILTSPSPSKSIPI
S270 ILTSPSPSKSIPIPQ
S272 TSPSPSKSIPIPQPF
S296‑p QFGQRDRsSsAPNVH
S297 FGQRDRsSsAPNVHI
S298‑p GQRDRsSsAPNVHIN
T306 APNVHINTIEPVNID
S327 GFRGDGGSTTGLSAt
T329 RGDGGSTTGLSAtPP
S332 GGSTTGLSAtPPASL
T334‑p STTGLSAtPPASLPG
S338 LSAtPPASLPGsLTN
S342‑p PPASLPGsLTNVKAL
K351 TNVKALQKSPGPQRE
S352 NVKALQKSPGPQRER
S361 GPQRERKSSSSSSSE
S362 PQRERKSSSSSSSED
S363 QRERKSSSSSSSEDR
S364 RERKSSSSSSSEDRS
S365 ERKSSSSSSSEDRSR
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  rat

 
- gap
S92 GGEHNPPSIYLEAYE
- gap
N130 TPTDVSRNNPKSPQK
S134 VSRNNPKSPQKPIVR
K137 NNPKSPQKPIVRVFL
T227 FVRKTFFTLAFCDFC
K236 AFCDFCRKLLFQGFR
S297 SAETTLPSGCSSAPP
C299 ETTLPSGCSSAPPSD
S300 TTLPSGCSSAPPSDS
S301 TLPSGCSSAPPSDSI
S315 IGPQILTSPSPSKSI
S317 PQILTSPSPSKSIPI
S319 ILTSPSPSKSIPIPQ
S321 TSPSPSKSIPIPQPF
S345‑p QFGQRDRsSsAPNVH
S346 FGQRDRsSsAPNVHI
S347‑p GQRDRsSsAPNVHIN
T355 APNVHINTIEPVNID
S428 PGPVFRGSTTGLSAT
T430 PVFRGSTTGLSATPP
S433 RGSTTGLSATPPASL
T435 STTGLSATPPASLPG
S439 LSATPPASLPGSLTN
S443 PPASLPGSLTNVKAL
K452 TNVKALQKSPGPQRE
S453 NVKALQKSPGPQRER
S462 GPQRERKSsSSSSST
S463‑p PQRERKSsSSSSSTE
S464 QRERKSsSSSSSTED
S465 RERKSsSSSSSTEDR
S466 ERKSsSSSSSTEDRS
T477 EDRSRMKTLGRRDss
S483‑p KTLGRRDssDDWEIP
S484‑p TLGRRDssDDWEIPD
T495 EIPDGQITVGQRIGS
S502 TVGQRIGSGSFGTVY
S504 GQRIGSGSFGTVYKG
T507 IGSGSFGTVYKGKWH
K615 SIIHRDLKSNNIFLH
S616 IIHRDLKSNNIFLHE
T636 IGDFGLATVKSRWSG
K638 DFGLATVKSRWSGSH
S639 FGLATVKSRWSGSHQ
S642 ATVKSRWSGSHQFEQ
S651 SHQFEQLSGSILWMA
S653 QFEQLSGSILWMAPE
S712 MVGRGYLSPDLSKVR
K724 KVRSNCPKAMKRLMA
S766‑p PKIHRSAsEPSLNRA
S769 HRSAsEPSLNRAGFQ
Y783 QTEDFSLYACASPKT
S787 FSLYACASPKTPIQA
T790 YACASPKTPIQAGGY
Y797 TPIQAGGYGGFPVH_
  chicken

 
S9‑p AALSSGSsAEGASLF
S76 GGEHNPPSIYLEAYE
T119 SSSASTDTVASSSSS
N147 NPTDMSRNNPKsPQK
S151‑p MSRNNPKsPQKPIVR
K154 NNPKsPQKPIVRVFL
T244 FVRKTFFTLAFCDFC
K253 AFCDFCRKLLFQGFR
S314 LGETTPASGSYPSVP
S316 ETTPASGSYPSVPPS
Y317 TTPASGSYPSVPPSD
S319 PASGSYPSVPPSDSV
S333‑p VGPPILPsPsPsKSI
S335‑p PPILPsPsPsKSIPI
S337‑p ILPsPsPsKSIPIPQ
S339 PsPsPsKSIPIPQPF
S363 QFGQRDRSSsAPNVH
S364 FGQRDRSSsAPNVHI
S365‑p GQRDRSSsAPNVHIN
T373 APNVHINTIEPVNID
S434 PGPVFRGSTAGLSAt
A436 PVFRGSTAGLSAtPP
S439 RGSTAGLSAtPPASL
T441‑p STAGLSAtPPASLPG
S445 LSAtPPASLPGSLTN
S449 PPASLPGSLTNVKAL
K458 TNVKALQKsPGPQRE
S459‑p NVKALQKsPGPQRER
S468 GPQRERKSSSSSEDR
S469 PQRERKSSSSSEDRN
S470 QRERKSSSSSEDRNR
S471 RERKSSSSSEDRNRM
S472 ERKSSSSSEDRNRMK
T480 EDRNRMKTLGRRDss
S486‑p KTLGRRDssDDWEIP
S487‑p TLGRRDssDDWEIPD
T498 EIPDGQITVGQRIGs
S505‑p TVGQRIGsGSFGTVY
S507 GQRIGsGSFGTVYKG
T510 IGsGSFGTVYKGKWH
K618 SIIHRDLKSNNIFLH
S619 IIHRDLKSNNIFLHE
T639 IGDFGLATVKSRWsG
K641 DFGLATVKSRWsGSH
S642 FGLATVKSRWsGSHQ
S645‑p ATVKSRWsGSHQFEQ
S654 SHQFEQLSGSILWMA
S656 QFEQLSGSILWMAPE
S715 MVGRGYLSPDLSKVR
K727 KVRSNCPKAMKRLMA
S769‑p PKIHRSAsEPSLNRA
S772 HRSAsEPSLNRAGFQ
Y786 QTEDFSLYACAsPKt
S790‑p FSLYACAsPKtPIQA
T793‑p YACAsPKtPIQAGGY
Y800 tPIQAGGYGGFPVH_
  quail

 
S9 AALSSGSSAEGASLF
S76 GGEHNPPSIYLEAYE
T119 SSSASTDTVASSSSS
N147 NPTDMSRNNPKSPQK
S151 MSRNNPKSPQKPIVR
K154 NNPKSPQKPIVRVFL
T244 FVRKTFFTLAFCDFC
K253 AFCDFCRKLLFQGFR
S314 LGETTPASGSYPSVP
S316 ETTPASGSYPSVPPS
Y317 TTPASGSYPSVPPSD
S319 PASGSYPSVPPSDSV
S333 VGPPILPSPSPSKSI
S335 PPILPSPSPSKSIPI
S337 ILPSPSPSKSIPIPQ
S339 PSPSPSKSIPIPQPF
S363 QFGQRDRSSSAPNVH
S364 FGQRDRSSSAPNVHI
S365 GQRDRSSSAPNVHIN
T373 APNVHINTIEPVNID
S434 PGPVFRGSTAGLSAT
A436 PVFRGSTAGLSATPP
S439 RGSTAGLSATPPASL
T441 STAGLSATPPASLPG
S445 LSATPPASLPGSLTN
S449 PPASLPGSLTNVKAL
K458 TNVKALQKSPGPQRE
S459 NVKALQKSPGPQRER
S468 GPQRERKSsSSSEDR
S469‑p PQRERKSsSSSEDRN
S470 QRERKSsSSSEDRNR
S471 RERKSsSSSEDRNRM
S472 ERKSsSSSEDRNRMK
T480 EDRNRMKTLGRRDsS
S486‑p KTLGRRDsSDDWEIP
S487 TLGRRDsSDDWEIPD
T498 EIPDGQITVGQRIGS
S505 TVGQRIGSGSFGTVY
S507 GQRIGSGSFGTVYKG
T510 IGSGSFGTVYKGKWH
K618 SIIHRDLKSNNIFLH
S619 IIHRDLKSNNIFLHE
T639 IGDFGLATVKSRWSG
K641 DFGLATVKSRWSGSH
S642 FGLATVKSRWSGSHQ
S645 ATVKSRWSGSHQFEQ
S654 SHQFEQLSGSILWMA
S656 QFEQLSGSILWMAPE
S715 MVGRGYLSPDLSKVR
K727 KVRSNCPKAMKRLMA
S769 PKIHRSASEPSLNRA
S772 HRSASEPSLNRAGFQ
Y786 QTEDFSLYACASPKT
S790 FSLYACASPKTPIQA
T793 YACASPKTPIQAGGY
Y800 TPIQAGGYGEFAAFK
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