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Protein Page:
Mena (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Mena Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes. Homotetramer. Interacts with APBB1IP, APBB1, PFN1 and ROBO4. Isoforms, containing the polyproline-rich regions with PPLP motifs, bind the WW domain of APBB1IP. Isoforms, containing the PPSY motif, bind, in vitro, to the WW2 and WW3 domains of NEDD4 and to the WW1 domain of YAP1. Binds the SH3 domain of BAIAP2-alpha but only after the autoinhibitory region of BAIAP2-alpha has been blocked by interaction with CDC42. Interacts, via the EVH1/WH1 domain, with the Pro-rich domains from VCL, ZYX and Listeria monocytogenes actA and with TES (via LIM domains). The TES LIM domain and the Pro-rich domains from VCL or ZYX compete for the same binding site. Interaction with ZYX is important for targeting ENAH to focal adhesions and enhances production of actin-rich structures at the apical surface of cells. Interacts, through the Pro-rich region, with the C-terminal SH3 domain of DNMPB. Binds GPHN. Interacts with FAT1 (via EVH1 domains). Heterotrimer with TES and ACTL7A. Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions. Belongs to the Ena/VASP family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 1q42.12
Cellular Component: cell junction; cytoplasm; cytoskeleton; cytosol; filopodium; focal adhesion; lamellipodium; plasma membrane; synapse
Molecular Function: actin binding; protein binding; SH3 domain binding; WW domain binding
Biological Process: axon guidance
Reference #:  Q8N8S7 (UniProtKB)
Alt. Names/Synonyms: ENA; enabled homolog (Drosophila); ENAH; MENA; NDPP1; Protein enabled homolog
Gene Symbols: ENAH
Molecular weight: 66,510 Da
Basal Isoelectric point: 6.51  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Mena

Protein Structure Not Found.
Download PyMol Script
Download ChimeraX Script


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 - gap
0 1 Y16‑p ARAAVMVyDDANKKW
0 1 K69 CAIPKGLKYNQATQT
0 2 K69‑ub CAIPKGLkYNQATQT
0 1 T116‑p EVLNSQEtGPtLPRQ
0 1 T119‑p NSQEtGPtLPRQNsQ
0 112 S125‑p PtLPRQNsQLPAQVQ
3 17 S265‑p WERERRIssAAAPAS
0 8 S266‑p ERERRIssAAAPASV
0 1 - gap
0 1 - gap
0 1 - gap
0 1 - gap
0 2 - gap
0 7 - gap
0 3 - gap
0 1 - gap
1 0 Q325 PLPPGPAQASVALPP
0 5 S405‑p GAKLRKVsRMEDtSF
0 1 T410‑p KVsRMEDtSFPsGGN
0 1 S414‑p MEDtSFPsGGNAIGV
0 1 S423‑p GNAIGVNsAssKTDT
0 1 S425‑p AIGVNsAssKTDTGR
0 1 S426‑p IGVNsAssKTDTGRG
0 6 R432 ssKTDTGRGNGPLPL
0 1 S442‑p GPLPLGGsGLMEEMS
0 1 K461‑ac RRRRIAEkGstIETE
0 1 S463‑p RRIAEkGstIETEQK
0 5 T464‑p RIAEkGstIETEQKE
0 2 S477‑p KEDKGEDsEPVtSKA
0 2 T481‑p GEDsEPVtSKAsstS
0 1 S482 EDsEPVtSKAsstSt
0 1 S485‑p EPVtSKAsstStPEP
0 1 S486‑p PVtSKAsstStPEPt
0 2 T487‑p VtSKAsstStPEPtR
0 1 S488 tSKAsstStPEPtRK
0 43 T489‑p SKAsstStPEPtRKP
0 1 T493‑p stStPEPtRKPWERt
0 10 T500‑p tRKPWERtNtMNGsK
0 51 T502‑p KPWERtNtMNGsKsP
0 25 S506‑p RtNtMNGsKsPVIsR
0 81 S508‑p NtMNGsKsPVIsRRD
0 9 S512‑p GsKsPVIsRRDsPRK
0 4 S516‑p PVIsRRDsPRKNQIV
0 2 S528‑p QIVFDNRsyDsLHRP
0 1 Y529‑p IVFDNRsyDsLHRPK
0 6 S531‑p FDNRsyDsLHRPKST
0 1 K574‑ub RKELTKLkEELIDAI
  Mena iso2  
- gap
Y16 ARAAVMVYDDANKKW
K69 CAIPKGLKYNQATQT
K69 CAIPKGLKYNQATQT
T116 EVLNSQETGPTLPRQ
T119 NSQETGPTLPRQNSQ
S125 PTLPRQNSQLPAQVQ
S265‑p WERERRIssAAAPAS
S266‑p ERERRIssAAAPASV
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
Q325 PLPPGPAQASVALPP
S405 GAKLRKVSRMEDTSF
T410 KVSRMEDTSFPSGGN
S414 MEDTSFPSGGNAIGV
S423 GNAIGVNSASSKTDT
S425 AIGVNSASSKTDTGR
S426 IGVNSASSKTDTGRG
R432 SSKTDTGRGNGPLPL
S442 GPLPLGGSGLMEEMS
K461 RRRRIAEKGSTIETE
S463 RRIAEKGSTIETEQK
T464 RIAEKGSTIETEQKE
S477 KEDKGEDSEPVTSKA
T481 GEDSEPVTSKASSTS
S482 EDSEPVTSKASSTSt
S485 EPVTSKASSTStPEP
S486 PVTSKASSTStPEPT
T487 VTSKASSTStPEPTR
S488 TSKASSTStPEPTRK
T489‑p SKASSTStPEPTRKP
T493 STStPEPTRKPWERt
T500‑p TRKPWERtNtMNGsK
T502‑p KPWERtNtMNGsKsP
S506‑p RtNtMNGsKsPVIsR
S508‑p NtMNGsKsPVIsRPK
S512‑p GsKsPVIsRPKSTPL
- gap
- gap
- gap
- gap
K553 RKELTKLKEELIDAI
  Mena iso3  
- gap
Y16 ARAAVMVYDDANKKW
K69 CAIPKGLKYNQATQT
K69 CAIPKGLKYNQATQT
T116 EVLNSQETGPTLPRQ
T119 NSQETGPTLPRQNSQ
S125 PTLPRQNSQLPAQVQ
S265 WERERRISSAGIVLG
S266 ERERRISSAGIVLGP
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
Q288 PLPPGPAQASVALPP
S368 GAKLRKVSRMEDTSF
T373 KVSRMEDTSFPSGGN
S377 MEDTSFPSGGNAIGV
S386 GNAIGVNSASSKTDT
S388 AIGVNSASSKTDTGR
S389 IGVNSASSKTDTGRG
R395 SSKTDTGRGNGPLPL
S405 GPLPLGGSGLMEEMS
K424 RRRRIAEKGSTIETE
S426 RRIAEKGSTIETEQK
T427 RIAEKGSTIETEQKE
S440 KEDKGEDSEPVTSKA
T444 GEDSEPVTSKASSTS
S445 EDSEPVTSKASSTST
S448 EPVTSKASSTSTPEP
S449 PVTSKASSTSTPEPT
T450 VTSKASSTSTPEPTR
S451 TSKASSTSTPEPTRK
T452 SKASSTSTPEPTRKP
T456 STSTPEPTRKPWERT
T463 TRKPWERTNTMNGSK
T465 KPWERTNTMNGSKSP
S469 RTNTMNGSKSPVISR
S471 NTMNGSKSPVISRPK
S475 GSKSPVISRPKSTPL
- gap
- gap
- gap
- gap
K516 RKELTKLKEELIDAI
  mouse

► Hide Isoforms
 
- gap
Y16 ARAAVMVYDDANKKW
K69 CAIPKGLKYNQATQT
K69‑ub CAIPKGLkYNQATQT
A116 EVLNSQEAAQSKVTA
T138 RCIFCGPTLPRQNsQ
S144‑p PTLPRQNsQLPAQVQ
S255‑p WERERRMsNAAPSSD
N256 ERERRMsNAAPSSDS
T315‑p PPTSTPPtPPLRHAA
T327‑p HAATRFAtsLGSAFH
S328‑p AATRFAtsLGSAFHP
S351‑p PRPLNKNsRPssPVN
S354‑p LNKNsRPssPVNTPS
S355‑p NKNsRPssPVNTPSS
S383‑p NFSPLPPsPPIMISs
S390‑p sPPIMISsPPGKATG
Y557 PLPSGPAYASALPPP
S637‑p GAKLRKVsRVEDGSF
G642 KVsRVEDGSFPGGGN
G646 VEDGSFPGGGNTGSV
L655 GNTGSVSLASSKADA
S657 TGSVSLASSKADAGr
S658 GSVSLASSKADAGrG
R664‑m1 SSKADAGrGNGPLPL
S674 GPLPLGGSGLMEEMS
K693 RRRRIAEKGStIETE
S695 RRIAEKGStIETEQK
T696‑p RIAEKGStIETEQKE
A709 KEDRNEDAEPITAKA
T713 NEDAEPITAKAPSts
A714 EDAEPITAKAPStst
P717 EPITAKAPStstPEP
S718 PITAKAPStstPEPT
T719‑p ITAKAPStstPEPTR
S720‑p TAKAPStstPEPTRK
T721‑p AKAPStstPEPTRKP
T725 StstPEPTRKPWERt
T732‑p TRKPWERtNtMNGsK
T734‑p KPWERtNtMNGsKsP
S738‑p RtNtMNGsKsPVIsR
S740‑p NtMNGsKsPVIsRPK
S744‑p GsKsPVIsRPKSTPS
- gap
- gap
- gap
- gap
K785 RKELAKLKEELIDAI
  Mena iso3  
- gap
Y16 ARAAVMVYDDANKKW
K69 CAIPKGLKYNQATQT
K69 CAIPKGLKYNQATQT
A116 EVLNSQEAGPTLPRQ
T119 NSQEAGPTLPRQNsQ
S125‑p PTLPRQNsQLPAQVQ
S236 WERERRMSNAAAPAS
N237 ERERRMSNAAAPASA
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
Y296‑p PLPSGPAyASALPPP
S376 GAKLRKVSRVEDGSF
G381 KVSRVEDGSFPGGGN
G385 VEDGSFPGGGNTGSV
L394 GNTGSVSLASSKADA
S396 TGSVSLASSKADAGR
S397 GSVSLASSKADAGRG
R403 SSKADAGRGNGPLPL
S413 GPLPLGGSGLMEEMS
K432 RRRRIAEKGSTIETE
S434 RRIAEKGSTIETEQK
T435 RIAEKGSTIETEQKE
A448 KEDRNEDAEPITAKA
T452 NEDAEPITAKAPSTS
A453 EDAEPITAKAPSTST
P456 EPITAKAPSTSTPEP
S457 PITAKAPSTSTPEPT
T458 ITAKAPSTSTPEPTR
S459 TAKAPSTSTPEPTRK
T460 AKAPSTSTPEPTRKP
T464 STSTPEPTRKPWERT
T471 TRKPWERTNTMNGSK
T473 KPWERTNTMNGSKSP
S477 RTNTMNGSKSPVISR
S479 NTMNGSKSPVISRPK
S483 GSKSPVISRPKSTPS
- gap
- gap
- gap
- gap
K524 RKELAKLKEELIDAI
  Mena iso4  
- gap
Y16 ARAAVMVYDDANKKW
K69 CAIPKGLKYNQATQT
K69 CAIPKGLKYNQATQT
A116 EVLNSQEAGPTLPRQ
T119 NSQEAGPTLPRQNsQ
S125‑p PTLPRQNsQLPAQVQ
S236‑p WERERRMsNAAPSSD
N237 ERERRMsNAAPSSDS
T296 PPTSTPPTPPLRHAA
T308 HAATRFATSLGSAFH
S309 AATRFATSLGSAFHP
S332 PRPLNKNSRPSSPVN
S335 LNKNSRPSSPVNTPS
S336 NKNSRPSSPVNTPSS
S364 NFSPLPPSPPIMISS
S371 SPPIMISSPPGKATG
Y538 PLPSGPAYASALPPP
S618 GAKLRKVSRVEDGSF
G623 KVSRVEDGSFPGGGN
G627 VEDGSFPGGGNTGSV
L636 GNTGSVSLASSKADA
S638 TGSVSLASSKADAGR
S639 GSVSLASSKADAGRG
R645 SSKADAGRGNGPLPL
S655 GPLPLGGSGLMEEMS
K674 RRRRIAEKGSTIETE
S676 RRIAEKGSTIETEQK
T677 RIAEKGSTIETEQKE
A690 KEDRNEDAEPITAKA
T694 NEDAEPITAKAPSTS
A695 EDAEPITAKAPSTST
P698 EPITAKAPSTSTPEP
S699 PITAKAPSTSTPEPT
T700 ITAKAPSTSTPEPTR
S701 TAKAPSTSTPEPTRK
T702 AKAPSTSTPEPTRKP
T706 STSTPEPTRKPWERT
T713 TRKPWERTNTMNGSK
T715 KPWERTNTMNGSKSP
S719 RTNTMNGSKSPVISR
S721 NTMNGSKSPVISRPK
S725 GSKSPVISRPKSTPS
- gap
- gap
- gap
- gap
K766 RKELAKLKEELIDAI
  rat

 
K12‑ac FVSYSIPkLRSSQSL
Y36 ARAAVMVYDDANKKW
K89‑ac CAIPKGLkYNQATQT
K89 CAIPKGLKYNQATQT
A136 EVLNSQEAGPTLPRQ
T139 NSQEAGPTLPRQNSQ
S145 PTLPRQNSQLPAQVQ
S258 WERERRMSNAGHVLG
N259 ERERRMSNAGHVLGP
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
Y281 PLPSGPAYASALPPP
S361 GAKLRKVSRVEDGSF
G366 KVSRVEDGSFPSGGN
S370 VEDGSFPSGGNTVSV
L379 GNTVSVNLASSKTDS
S381 TVSVNLASSKTDSGR
S382 VSVNLASSKTDSGRG
R388 SSKTDSGRGNGPLPL
S398 GPLPLGGSGLMEEMS
K417 RRRRIAEKGSTIETE
S419 RRIAEKGSTIETEQK
T420 RIAEKGSTIETEQKE
A433 KEDRSEDAEPVtsKA
T437‑p SEDAEPVtsKAASTS
S438‑p EDAEPVtsKAASTST
A441 EPVtsKAASTSTPEP
S442 PVtsKAASTSTPEPT
T443 VtsKAASTSTPEPTR
S444 tsKAASTSTPEPTRK
T445 sKAASTSTPEPTRKP
T449 STSTPEPTRKPWERT
T456 TRKPWERTNTMNGSK
T458 KPWERTNTMNGSKsP
S462 RTNTMNGSKsPVISR
S464‑p NTMNGSKsPVISRPK
S468 GSKsPVISRPKSTPS
- gap
- gap
- gap
- gap
K509 RKELAKLKEELIDAI
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