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Protein Page:
PU.1 (human)

Overview
PU.1 Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing. Binds DNA as a monomer. Interacts with CEBPD and NONO. Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Highly expressed in both FV-P and FV-A-induced erythro- leukemia cell lines that have undergone rearrangements of the Spi- 1 gene due to the insertion of SFFV. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: DNA-binding; Oncoprotein; Transcription factor
Chromosomal Location of Human Ortholog: 11p11.2
Cellular Component: nuclear chromatin
Molecular Function: NFAT protein binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity
Biological Process: negative regulation of gene expression, epigenetic; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of erythrocyte differentiation
Reference #:  P17947 (UniProtKB)
Alt. Names/Synonyms: 31 kDa transforming protein; 31 kDa-transforming protein; hematopoietic transcription factor PU.1; OF; PU.1; SFPI1; SPI-1; SPI-1 proto-oncogene; SPI-A; SPI1; spleen focus forming virus (SFFV) proviral integration oncogene spi1; Transcription factor PU.1
Gene Symbols: SPI1
Molecular weight: 31,083 Da
Basal Isoelectric point: 6.31  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PU.1

Protein Structure Not Found.
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Modification Sites and Domains  

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S17‑p FPLVPPPsEDLVPYD
1 0 S41 EYYPYLSSDGESHSD
0 1 S125 CLQYPSLSPAQPSSD
1 1 S130 SLSPAQPSSDEEEGE
1 1 S131 LSPAQPSSDEEEGER
1 2 S140‑p EEEGERQsPPLEVsD
6 3 S146‑p QsPPLEVsDGEADGL
  mouse

 
S17 FSLTAPPSDDLVTYD
S41‑p DYYSFVGsDGESHSD
S126‑p CFPYQTLsPAHQQss
S132‑p LsPAHQQssDEEEGE
S133‑p sPAHQQssDEEEGER
S142‑p EEEGERQsPPLEVsD
S148‑p QsPPLEVsDGEADGL
  rat

 
S17 FPLVAPPSDELVTYE
G41 DYYSLIGGDGDSHSD
S125 CFPYPPLSPAHQQSS
S131 LSPAHQQSSDEEEGE
S132 SPAHQQSSDEEEGER
S141 EEEGERQSPPLEVsD
S147‑p QSPPLEVsDGEADGL
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