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Protein Page:
Chk2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Chk2 a CAMK protein kinase of the Rad53 family. A cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. Phosphorylates and inhibits CDC25C, preventing entry into mitosis, p53, leading to cell cycle arrest in G1, and BRCA1, restoring survival after DNA damage. Twelve splice variant isoforms have been described for human Chk2. LOF mutants cause Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype also caused by p53 mutations. Familial mutations also associated with prostate and breast cancer, and mutations also seen in a variety of sporadic cancers and cell lines. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, CAMK; Tumor suppressor; Protein kinase, Ser/Thr (non-receptor); CAMK group; RAD53 family
Chromosomal Location of Human Ortholog: 22q12.1
Cellular Component: chromosome, telomeric region; Golgi apparatus; nucleoplasm; PML body
Molecular Function: ATP binding; identical protein binding; metal ion binding; protein binding; protein homodimerization activity; protein kinase binding; protein serine/threonine kinase activity; ubiquitin protein ligase binding
Biological Process: cell division; cellular protein catabolic process; DNA damage checkpoint; DNA damage induced protein phosphorylation; DNA damage response, signal transduction; DNA damage response, signal transduction resulting in induction of apoptosis; DNA repair; double-strand break repair; G2/M transition of mitotic cell cycle; peptidyl-serine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein stabilization; regulation of protein catabolic process; regulation of transcription, DNA-dependent; replicative cell aging; response to DNA damage stimulus; response to gamma radiation; transcription, DNA-dependent
Disease: Breast Cancer; Li-fraumeni Syndrome 2; Osteogenic Sarcoma; Prostate Cancer
Reference #:  O96017 (UniProtKB)
Alt. Names/Synonyms: Cds1; checkpoint-like protein CHK2; CHEK2; CHK2; CHK2 checkpoint homolog (S. pombe); HuCds1; LFS2; PP1425; protein kinase CHK2; RAD53; Serine/threonine-protein kinase Chk2
Gene Symbols: CHEK2
Molecular weight: 60,915 Da
Basal Isoelectric point: 5.65  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  G1/S Checkpoint  |  G2/M DNA Damage Checkpoint
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Chk2

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
apoptosis, induced: T68‑p, S379‑p, S456‑p
cell cycle regulation: S19‑p, S33‑p, S35‑p, T68‑p, S73‑p, S164‑p, T205‑p, S210‑p, T387‑p
cytoskeletal reorganization: T68‑p
enzymatic activity, induced: S19‑p, T26‑p, S28‑p, S33‑p, S35‑p, T68‑p, S73‑p, S140‑p, S379‑p, T383‑p, T387‑p, Y390‑p, S516‑p
enzymatic activity, inhibited: S164‑p, T205‑p, S210‑p
intracellular localization: T68‑p, S372‑p, T378‑p, S379‑p, T383‑p, T387‑p, T389‑p, Y390‑p
molecular association, regulation: S19‑p, S33‑p, S35‑p, T68‑p, S73‑p
phosphorylation: T26‑p, S28‑p, T68‑p, S73‑p, T389‑p
protein conformation: T68‑p, S140‑p, T383‑p, T387‑p
protein degradation: S456‑p
ubiquitination: S379‑p, T383‑p, S456‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S12‑p SDVEAQQsHGsSACs
0 1 S15‑p EAQQsHGsSACsQPH
11 1 S19‑p sHGsSACsQPHGsVt
0 1 S24‑p ACsQPHGsVtQsQGS
3 1 T26‑p sQPHGsVtQsQGSSs
3 0 S28‑p PHGsVtQsQGSSsQs
11 1 S33‑p tQsQGSSsQsQGIss
10 1 S35‑p sQGSSsQsQGIssss
0 1 S39‑p SsQsQGIsssststM
0 1 S40‑p sQsQGIsssststMP
0 1 S41‑p QsQGIsssststMPN
0 1 S42‑p sQGIsssststMPNS
0 1 T43‑p QGIsssststMPNSs
0 1 S44‑p GIsssststMPNSsQ
0 1 T45‑p IsssststMPNSsQs
0 1 S50‑p tstMPNSsQsSHsSS
0 1 S52‑p tMPNSsQsSHsSSGT
0 1 S55‑p NSsQsSHsSSGTLSs
1 0 S62‑p sSSGTLSsLEtVstQ
0 1 T65‑p GTLSsLEtVstQELY
0 1 S67‑p LSsLEtVstQELYsI
156 3 T68‑p SsLEtVstQELYsIP
1 1 S73‑p VstQELYsIPEDQEP
3 4 S120‑p YWFGRDKsCEYCFDE
1 0 S140‑p TDKYRTYsKKHFRIF
1 1 S164‑p IAYIEDHsGNGTFVN
1 1 T205‑p VFVFFDLtVDDQsVY
1 1 S210‑p DLtVDDQsVYPKALR
1 1 T225‑p DEYIMSKtLGSGACG
0 1 K235‑ac SGACGEVkLAFERKT
2 6 S260‑p KRKFAIGsAREADPA
1 1 S372‑p KITDFGHsKILGEts
2 2 T378‑p HsKILGEtsLMRtLC
5 7 S379‑p sKILGEtsLMRtLCG
8 3 T383‑p GEtsLMRtLCGtPty
17 3 T387‑p LMRtLCGtPtyLAPE
2 1 T389‑p RtLCGtPtyLAPEVL
1 1 Y390‑p tLCGtPtyLAPEVLV
4 2 T432‑p PPFSEHRtQVsLKDQ
1 2 S435‑p SEHRtQVsLKDQITS
1 0 S456‑p PEVWAEVsEKALDLV
14 3 S516‑p PQVLAQPstSRKRPR
1 0 T517‑p QVLAQPstSRKRPRE
0 2 A537 AETTKRPAVCAAVL_
2666 : Phospho-Chk2 (Ser19) Antibody
2665 : Phospho-Chk2 (Ser33/35) Antibody
2665 : Phospho-Chk2 (Ser33/35) Antibody
2197 : Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb
2661 : Phospho-Chk2 (Thr68) Antibody
12812 : Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb (PE Conjugate)
2669 : Phospho-Chk2 (Ser516) Antibody
  Chk2 iso9  
S12 SDVEAQQSHGSSACS
S15 EAQQSHGSSACSQPH
S19 SHGSSACSQPHGSVT
S24 ACSQPHGSVTQSQGS
T26 SQPHGSVTQSQGSSS
S28 PHGSVTQSQGSSSQS
S33 TQSQGSSSQSQGISS
S35 SQGSSSQSQGISSSS
S39 SSQSQGISSSSTSTM
S40 SQSQGISSSSTSTMP
S41 QSQGISSSSTSTMPN
S42 SQGISSSSTSTMPNS
T43 QGISSSSTSTMPNSS
S44 GISSSSTSTMPNSSQ
T45 ISSSSTSTMPNSSQS
S50 TSTMPNSSQSSHSSS
S52 TMPNSSQSSHSSSGT
S55 NSSQSSHSSSGTLSS
S62 SSSGTLSSLETVSTQ
T65 GTLSSLETVSTQELY
S67 LSSLETVSTQELYSI
T68 SSLETVSTQELYSIP
S73 VSTQELYSIPEDQEP
S163 YWFGRDKSCEYCFDE
S183 TDKYRTYSKKHFRIF
S207 IAYIEDHSGNGTFVN
T248 VFVFFDLTVDDQSVY
S253 DLTVDDQSVYPKALR
T268 DEYIMSKTLGSGACG
K278 SGACGEVKLAFERKT
S303 KRKFAIGSAREADPA
S415 KITDFGHSKILGETS
T421 HSKILGETSLMRTLC
S422 SKILGETSLMRTLCG
T426 GETSLMRTLCGTPTY
T430 LMRTLCGTPTYLAPE
T432 RTLCGTPTYLAPEVL
Y433 TLCGTPTYLAPEVLV
T475 PPFSEHRTQVSLKDQ
S478 SEHRTQVSLKDQITS
S499 PEVWAEVSEKALDLV
S559 PQVLAQPSTSRKRPR
T560 QVLAQPSTSRKRPRE
A580 AETTKRPAVCAAVL_
  Chk2 iso11  
S12 SDVEAQQSHGSSACS
S15 EAQQSHGSSACSQPH
S19 SHGSSACSQPHGSVT
S24 ACSQPHGSVTQSQGS
T26 SQPHGSVTQSQGSSS
S28 PHGSVTQSQGSSSQS
S33 TQSQGSSSQSQGISS
S35 SQGSSSQSQGISSSS
S39 SSQSQGISSSSTSTM
S40 SQSQGISSSSTSTMP
S41 QSQGISSSSTSTMPN
S42 SQGISSSSTSTMPNS
T43 QGISSSSTSTMPNSS
S44 GISSSSTSTMPNSSQ
T45 ISSSSTSTMPNSSQS
S50 TSTMPNSSQSSHSSS
S52 TMPNSSQSSHSSSGT
S55 NSSQSSHSSSGTLSS
S62 SSSGTLSSLETVSTQ
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
T65 GTLSSLETVSTQELY
Q69 SLETVSTQELYSIPE
L71 ETVSTQELYSIPEVL
Y72 TVSTQELYSIPEVLV
T114 PPFSEHRTQVSLKDQ
S117 SEHRTQVSLKDQITS
S138 PEVWAEVSEKALDLV
S198 PQVLAQPSTSRKRPR
T199 QVLAQPSTSRKRPRE
A219 AETTKRPAVCAAVL_
  Chk2 iso12  
S12 SDVEAQQSHGSSACS
S15 EAQQSHGSSACSQPH
S19 SHGSSACSQPHGSVT
S24 ACSQPHGSVTQSQGS
T26 SQPHGSVTQSQGSSS
S28 PHGSVTQSQGSSSQS
S33 TQSQGSSSQSQGISS
S35 SQGSSSQSQGISSSS
S39 SSQSQGISSSSTSTM
S40 SQSQGISSSSTSTMP
S41 QSQGISSSSTSTMPN
S42 SQGISSSSTSTMPNS
T43 QGISSSSTSTMPNSS
S44 GISSSSTSTMPNSSQ
T45 ISSSSTSTMPNSSQS
S50 TSTMPNSSQSSHSSS
S52 TMPNSSQSSHSSSGT
S55 NSSQSSHSSSGTLSS
S62 SSSGTLSSLETVSTQ
T65 GTLSSLETVSTQELY
S67 LSSLETVSTQELYSI
T68 SSLETVSTQELYSIP
S73 VSTQELYSIPEDQEP
S120 YWFGRDKSCEYCFDE
S140 TDKYRTYSKKHFRIF
S164 IAYIEDHSGNGTFVN
T205 VFVFFDLTVDDQSVY
S210 DLTVDDQSVYPKALR
T225 DEYIMSKTLGSGACG
K235 SGACGEVKLAFERKT
S260 KRKFAIGSAREADPA
S343 QITDFGHSKILGETS
T349 HSKILGETSLMRTLC
S350 SKILGETSLMRTLCG
T354 GETSLMRTLCGTPTY
T358 LMRTLCGTPTYLAPE
T360 RTLCGTPTYLAPEVL
Y361 TLCGTPTYLAPEVLV
T403 PPFSEHRTQVSLKDQ
S406 SEHRTQVSLKDQITS
S427 PEVWAEVSEKALDLV
S487 PQVLAQPSTSRKRPR
T488 QVLAQPSTSRKRPRE
A508 AETTKRPAVCAAVL_
  mouse

 
A15 HSSTSSKAHDSASCS
S18 TSSKAHDSASCSQSQ
S22 AHDSASCSQSQGGFS
G27 SCSQSQGGFSQPQGT
S29 SQSQGGFSQPQGTPS
P31 SQGGFSQPQGTPSQL
S36 SQPQGTPSQLHELSQ
S42 PSQLHELSQYQGSSS
S48 LSQYQGSSSSSTGTV
S49 SQYQGSSSSSTGTVP
S50 QYQGSSSSSTGTVPS
S51 YQGSSSSSTGTVPSS
T52 QGSSSSSTGTVPSSS
G53 GSSSSSTGTVPSSSQ
T54 SSSSSTGTVPSSSQS
S59 TGTVPSSSQSSHSSS
S61 TVPSSSQSSHSSSGT
S64 SSSQSSHSSSGTLSS
S71 SSSGTLSSLETVStQ
T74 GTLSSLETVStQELC
S76 LSSLETVStQELCSI
T77‑p SSLETVStQELCSIP
S82 VStQELCSIPEDQEP
S124 YWFGRDKSCEYCFDG
S144 TDKYRTYSKKHFRIF
S168 IVYIEDHSGNGTFVN
T209 VFVFFDLTVDDQSVY
S214 DLTVDDQSVYPKELR
T229 DEYIMSKTLGSGACG
K239 SGACGEVKMAFERKT
S264 KRRFALGSSREADTA
S376 KITDFGQSKILGETs
T382 QSKILGETsLMRTLC
S383‑p SKILGETsLMRTLCG
T387 GETsLMRTLCGTPTY
T391 LMRTLCGTPTYLAPE
T393 RTLCGTPTYLAPEVL
Y394 TLCGTPTYLAPEVLV
T436 PPFSEHKTQVSLKDQ
S439 SEHKTQVSLKDQITS
S460 PEVWTDVSEEALDLV
S520 PVAPAQTSSQKRPLE
- gap
S540‑p MPSTKRLsVCGAVL_
  rat

 
A14 HGSTSSKAHDSASCS
S17 TSSKAHDSASCSQSQ
S21 AHDSASCSQSQGGFS
G26 SCSQSQGGFSQSQGT
S28 SQSQGGFSQSQGTPS
S30 SQGGFSQSQGTPSQL
S35 SQSQGTPSQLHDLSQ
S41 PSQLHDLSQYQGASS
S47 LSQYQGASSSSTSTV
S48 SQYQGASSSSTSTVP
S49 QYQGASSSSTSTVPS
S50 YQGASSSSTSTVPSS
T51 QGASSSSTSTVPSSS
S52 GASSSSTSTVPSSSQ
T53 ASSSSTSTVPSSSQS
S58 TSTVPSSSQSSHSSS
S60 TVPSSSQSSHSSSGT
S63 SSSQSSHSSSGTLSS
S70 SSSGTLSSLETVSTQ
T73 GTLSSLETVSTQELC
S75 LSSLETVSTQELCSI
T76 SSLETVSTQELCSIP
S81 VSTQELCSIPEDQEP
S123 YWFGRDKSCEYCFDG
S143 TDKYRTYSKKHFRIF
S167 IVYLEDHSGNGTFVN
T208 VFVFFDLTVDDQSVY
S213 DLTVDDQSVYPKELR
T228 DEYIMSKTLGSGACG
K238 SGACGEVKMAFERKT
S263 KRRFALGSSREADTA
S375 KITDFGQSKILGETS
T381 QSKILGETSLMRTLC
S382 SKILGETSLMRTLCG
T386 GETSLMRTLCGTPTY
T390 LMRTLCGTPTYLAPE
T392 RTLCGTPTYLAPEVL
Y393 TLCGTPTYLAPEVLI
T435 PPFSEHKTQVSLKDQ
S438 SEHKTQVSLKDQITS
S459 PEVWTDVSEKALDLV
S519 PLAPAQTSGQKRPLE
- gap
A539 AESSKRLAVCKAVL_
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