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Protein Page:
ACTN1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
ACTN1 a cytoskeletal protein of the spectrin superfamily. An actin-binding protein with multiple roles in different cell types. A nonmuscle isoform structurally similar to the erythroid beta spectrin gene. Note: This description may include information from UniProtKB.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 14q24|14q22-q24
Cellular Component: actin filament; brush border; cell projection; cytoplasm; cytosol; extracellular region; extracellular space; fascia adherens; focal adhesion; intercellular junction; intracellular; plasma membrane; pseudopodium; ruffle; stress fiber; Z disc
Molecular Function: actin filament binding; calcium ion binding; double-stranded RNA binding; integrin binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; protein homodimerization activity; vinculin binding
Biological Process: actin crosslink formation; actin filament bundle formation; actin filament network formation; actin filament organization; focal adhesion formation; negative regulation of cell motility; platelet activation; platelet degranulation; platelet formation; regulation of apoptosis
Disease: Bleeding Disorder, Platelet-type, 15
Reference #:  P12814 (UniProtKB)
Alt. Names/Synonyms: actinin 1 smooth muscle; actinin, alpha 1; ACTN1; alpha-actinin 1; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; FLJ40884; FLJ54432; Non-muscle alpha-actinin-1
Gene Symbols: ACTN1
Molecular weight: 103,058 Da
Basal Isoelectric point: 5.25  Predict pI for various phosphorylation states
CST Pathways:  T Cell Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ACTN1

Protein Structure Not Found.
Download PyMol Script
Download ChimeraX Script


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Sites Implicated In
cell motility, altered: Y12‑p
cytoskeletal reorganization: Y12‑p
molecular association, regulation: Y12‑p
phosphorylation: Y12‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 Y4‑p ____MDHyDsQQtND
0 2 S6‑p __MDHyDsQQtNDyM
0 1 T9‑p DHyDsQQtNDyMQPE
4 3 Y12‑p DsQQtNDyMQPEEDW
0 3 K35‑ub AWEKQQRkTFTAWCN
0 4 T50‑p SHLRKAGtQIENIEE
0 1 K89 RGKMRVHKIsNVNkA
0 3 S91‑p KMRVHKIsNVNkALD
0 2 K95‑ac HKIsNVNkALDFIAs
0 3 S102‑p kALDFIAsKGVKLVS
0 22 S140‑p RFAIQDIsVEETSAK
0 375 Y193‑p HRPELIDyGkLRkDD
0 2 K195‑ac PELIDyGkLRkDDPL
0 1 K195 PELIDyGKLRkDDPL
0 1 K198‑ac IDyGkLRkDDPLTNL
0 1 K198 IDyGkLRKDDPLTNL
0 20 Y215‑p AFDVAEKyLDIPKML
0 2 T230‑p DAEDIVGtARPDEKA
0 3 Y241‑p DEKAIMTyVssFyHA
0 2 S243‑p KAIMTyVssFyHAFs
0 27 S244‑p AIMTyVssFyHAFsG
0 519 Y246‑p MTyVssFyHAFsGAQ
0 5 S250‑p ssFyHAFsGAQKAET
0 1 Y279‑p NEQLMEDyEKLAsDL
0 3 S284‑p EDyEKLAsDLLEWIR
0 48 K331‑ub KPPKVQEkCQLEINF
0 3 S362‑p PSEGRMVsDINNAWG
0 1 Y378‑p LEQVEKGyEEWLLNE
0 5 K398‑ac RLDHLAEkFRQkAsI
0 3 K398‑ub RLDHLAEkFRQkAsI
0 3 K402‑ub LAEkFRQkAsIHEAW
1 5 S404‑p EkFRQkAsIHEAWtD
0 2 T410‑p AsIHEAWtDGKEAML
0 2 K420 KEAMLRQKDyETATL
0 1 Y422‑p AMLRQKDyETATLSE
0 2 S442‑p KKHEAFEsDLAAHQD
0 1 Y466‑p QELNELDyyDSPsVN
0 1 Y467‑p ELNELDyyDSPsVNA
0 1 S471‑p LDyyDSPsVNARCQK
0 1 T490 WDNLGALTQkRREAL
0 2 K492‑ac NLGALTQkRREALER
0 1 Y511‑p LETIDQLyLEyAKRA
0 1 Y514‑p IDQLyLEyAKRAAPF
0 3 K516 QLyLEyAKRAAPFNN
0 1 K563 ATLPDADKERLAILG
0 1 Y582‑p VSKIVQTyHVNMAGT
0 2 K633‑ub QHNERLRkQFGAQAN
0 1 K649 IGPWIQTKMEEIGRI
0 1 S669‑p GTLEDQLsHLRQyEk
0 5 Y674‑p QLsHLRQyEksIVNy
0 2 K676‑ac sHLRQyEksIVNyKP
0 3 S677‑p HLRQyEksIVNyKPK
0 19 Y681‑p yEksIVNyKPKIDQL
0 2 Y708‑p FDNKHTNyTMEHIRV
0 2 T722‑p VGWEQLLtTIARTIN
0 2 T737‑p EVENQILtRDAKGIs
0 1 K741 QILtRDAKGIsQEQM
0 2 S744‑p tRDAKGIsQEQMNEF
0 1 - gap
0 1 S763‑p NHFDRDHsGtLGPEE
0 1 T765‑p FDRDHsGtLGPEEFK
0 2 S797‑p AEFARIMsIVDPNRL
0 1 T820‑p IDFMSREtADtDtAD
0 1 T823‑p MSREtADtDtADQVM
0 1 T825‑p REtADtDtADQVMAs
0 2 S832‑p tADQVMAsFKILAGD
0 4 Y842‑p ILAGDKNyItMDELR
0 1 T844‑p AGDKNyItMDELRRE
0 1 Y887‑p MSFSTALyGEsDL__
0 6 S890‑p STALyGEsDL_____
  ACTN1 iso2  
Y4 ____MDHYDSQQTND
S6 __MDHYDSQQTNDYM
T9 DHYDSQQTNDYMQPE
Y12 DSQQTNDYMQPEEDW
K35 AWEKQQRKTFTAWCN
T50 SHLRKAGTQIENIEE
K89 RGKMRVHKISNVNKA
S91 KMRVHKISNVNKALD
K95 HKISNVNKALDFIAS
S102 KALDFIASKGVKLVS
S140 RFAIQDISVEETSAK
Y193 HRPELIDYGKLRKDD
K195 PELIDYGKLRKDDPL
K195 PELIDYGKLRKDDPL
K198 IDYGKLRKDDPLTNL
K198 IDYGKLRKDDPLTNL
Y215 AFDVAEKYLDIPKML
T230 DAEDIVGTARPDEKA
Y241 DEKAIMTYVSSFYHA
S243 KAIMTYVSSFYHAFS
S244 AIMTYVSSFYHAFSG
Y246 MTYVSSFYHAFSGAQ
S250 SSFYHAFSGAQKAET
Y279 NEQLMEDYEKLASDL
S284 EDYEKLASDLLEWIR
K331 KPPKVQEKCQLEINF
S362 PSEGRMVSDINNAWG
Y378 LEQVEKGYEEWLLNE
K398 RLDHLAEKFRQKASI
K398 RLDHLAEKFRQKASI
K402 LAEKFRQKASIHEAW
S404 EKFRQKASIHEAWTD
T410 ASIHEAWTDGKEAML
K420 KEAMLRQKDYETATL
Y422 AMLRQKDYETATLSE
S442 KKHEAFESDLAAHQD
Y466 QELNELDYYDSPSVN
Y467 ELNELDYYDSPSVNA
S471 LDYYDSPSVNARCQK
T490 WDNLGALTQKRREAL
K492 NLGALTQKRREALER
Y511 LETIDQLYLEYAKRA
Y514 IDQLYLEYAKRAAPF
K516 QLYLEYAKRAAPFNN
K563 ATLPDADKERLAILG
Y582 VSKIVQTYHVNMAGT
K633 QHNERLRKQFGAQAN
K649 IGPWIQTKMEEIGRI
S669 GTLEDQLSHLRQYEK
Y674 QLSHLRQYEKSIVNY
K676 SHLRQYEKSIVNYKP
S677 HLRQYEKSIVNYKPK
Y681 YEKSIVNYKPKIDQL
Y708 FDNKHTNYTMEHIRV
T722 VGWEQLLTTIARTIN
T737 EVENQILTRDAKGIS
K741 QILTRDAKGISQEQM
S744 TRDAKGISQEQMNEF
K762 FNHFDRKKTGMMDTD
- gap
- gap
S792 AEFARIMSIVDPNRL
T815 IDFMSRETADTDTAD
T818 MSRETADTDTADQVM
T820 RETADTDTADQVMAS
S827 TADQVMASFKILAGD
Y837 ILAGDKNYITMDELR
T839 AGDKNYITMDELRRE
Y882 MSFSTALYGESDL__
S885 STALYGESDL_____
  mouse

► Hide Isoforms
 
Y4 ____MDHYDSQQTND
S6 __MDHYDSQQTNDYM
T9 DHYDSQQTNDYMQPE
Y12 DSQQTNDYMQPEEDW
K35‑ub AWEKQQRkTFTAWCN
T50 SHLRKAGTQIENIEE
K89 RGKMRVHKIsNVNKA
S91‑p KMRVHKIsNVNKALD
K95 HKIsNVNKALDFIAS
S102 KALDFIASKGVKLVS
S140‑p RFAIQDIsVEETSAK
Y193‑p HRPELIDyGkLRkDD
K195 PELIDyGKLRkDDPL
K195‑ub PELIDyGkLRkDDPL
K198 IDyGkLRKDDPLTNL
K198‑ub IDyGkLRkDDPLTNL
F215 AFDVAERFLDIPKML
T230 DAEDIVGTARPDEKA
Y241 DEKAIMTYVSsFyHA
S243 KAIMTYVSsFyHAFs
S244‑p AIMTYVSsFyHAFsG
Y246‑p MTYVSsFyHAFsGAQ
S250‑p SsFyHAFsGAQKAET
Y279 NEQLMEDYEKLAsDL
S284‑p EDYEKLAsDLLEWIR
K331‑ub KPPKVQEkCQLEINF
S362‑p PSEGRMVsDINNAWG
Y378 LEQAEKGYEEWLLNE
K398‑ac RLDHLAEkFRQkAsI
K398‑ub RLDHLAEkFRQkAsI
K402‑ub LAEkFRQkAsIHEAW
S404‑p EkFRQkAsIHEAWtD
T410‑p AsIHEAWtDGKEAML
K420‑ac KEAMLRQkDYETATL
Y422 AMLRQkDYETATLSE
S442 KKHEAFESDLAAHQD
Y466 QELNELDYYDSPSVN
Y467 ELNELDYYDSPSVNA
S471 LDYYDSPSVNARCQK
T490‑p WDNLGALtQkRREAL
K492‑ac NLGALtQkRREALER
Y511 LETIDQLYLEYAkRA
Y514 IDQLYLEYAkRAAPF
K516‑ac QLYLEYAkRAAPFNN
K563 ATLPDADKERLAILG
Y582 VSKIVQTYHVNMAGT
K633‑ub QHNERLRkQFGAQAN
K649‑ac IGPWIQTkMEEIGRI
S669 GTLEDQLSHLRQYEK
Y674 QLSHLRQYEKsIVNy
K676 SHLRQYEKsIVNyKP
S677‑p HLRQYEKsIVNyKPK
Y681‑p YEKsIVNyKPKIDQL
Y708 FDNKHTNYNMEHIRV
T722 VGWEQLLTTIARTIN
T737 EVENQILTRDAkGIS
K741‑ub QILTRDAkGISQEQM
S744 TRDAkGISQEQMNEF
- gap
S763 NHFDRDHSGTLGPEE
T765 FDRDHSGTLGPEEFK
S797 AEFARIMSIVDPNRL
T820 IDFMSRETADTDTAD
T823 MSRETADTDTADQVM
T825 RETADTDTADQVMAS
S832 TADQVMASFKILAGD
Y842 ILAGDKNYITEDELR
T844 AGDKNYITEDELRRE
Y887 MSFSTALYGEsDL__
S890‑p STALYGEsDL_____
  ACTN1 iso3  
Y4 ____MDHYDSQQTND
S6 __MDHYDSQQTNDYM
T9 DHYDSQQTNDYMQPE
Y12 DSQQTNDYMQPEEDW
K35 AWEKQQRKTFTAWCN
T50 SHLRKAGTQIENIEE
K89 RGKMRVHKISNVNKA
S91 KMRVHKISNVNKALD
K95 HKISNVNKALDFIAS
S102 KALDFIASKGVKLVS
S140 RFAIQDISVEETSAK
Y193 HRPELIDYGKLRKDD
K195 PELIDYGKLRKDDPL
K195 PELIDYGKLRKDDPL
K198 IDYGKLRKDDPLTNL
K198 IDYGKLRKDDPLTNL
F215 AFDVAERFLDIPKML
T230 DAEDIVGTARPDEKA
Y241 DEKAIMTYVSSFYHA
S243 KAIMTYVSSFYHAFS
S244 AIMTYVSSFYHAFSG
Y246 MTYVSSFYHAFSGAQ
S250 SSFYHAFSGAQKAET
Y279 NEQLMEDYEKLASDL
S284 EDYEKLASDLLEWIR
K331 KPPKVQEKCQLEINF
S362 PSEGRMVSDINNAWG
Y378 LEQAEKGYEEWLLNE
K398 RLDHLAEKFRQKASI
K398 RLDHLAEKFRQKASI
K402 LAEKFRQKASIHEAW
S404 EKFRQKASIHEAWTD
T410 ASIHEAWTDGKEAML
K420 KEAMLRQKDYETATL
Y422 AMLRQKDYETATLSE
S442 KKHEAFESDLAAHQD
Y466 QELNELDYYDSPSVN
Y467 ELNELDYYDSPSVNA
S471 LDYYDSPSVNARCQK
T490 WDNLGALTQKRREAL
K492 NLGALTQKRREALER
Y511 LETIDQLYLEYAKRA
Y514 IDQLYLEYAKRAAPF
K516 QLYLEYAKRAAPFNN
K563 ATLPDADKERLAILG
Y582 VSKIVQTYHVNMAGT
K633 QHNERLRKQFGAQAN
K649 IGPWIQTKMEEIGRI
S669 GTLEDQLSHLRQYEK
Y674 QLSHLRQYEKSIVNY
K676 SHLRQYEKSIVNYKP
S677 HLRQYEKSIVNYKPK
Y681 YEKSIVNYKPKIDQL
Y708 FDNKHTNYNMEHIRV
T722 VGWEQLLTTIARTIN
T737 EVENQILTRDAKGIS
K741 QILTRDAKGISQEQM
S744 TRDAKGISQEQMNEF
K762‑ub FNHFDRKkTGMMDTD
- gap
- gap
S792 AEFARIMSIVDPNRL
T815 IDFMSRETADTDTAD
T818 MSRETADTDTADQVM
T820 RETADTDTADQVMAS
S827 TADQVMASFKILAGD
Y837 ILAGDKNYITEDELR
T839 AGDKNYITEDELRRE
Y882 MSFSTALYGESDL__
S885 STALYGESDL_____
  rat

 
Y4 ____MDHYDSQQTND
S6 __MDHYDSQQTNDYM
T9 DHYDSQQTNDYMQPE
Y12 DSQQTNDYMQPEEDW
K35 AWEKQQRKTFTAWCN
T50‑p SHLRKAGtQIENIEE
K89‑ac RGKMRVHkISNVNKA
S91 KMRVHkISNVNKALD
K95 HkISNVNKALDFIAs
S102‑p KALDFIAsKGVKLVS
S140‑p RFAIQDIsVEETSAK
Y193 HRPELIDYGkLRKDD
K195‑ac PELIDYGkLRKDDPL
K195 PELIDYGKLRKDDPL
K198 IDYGkLRKDDPLTNL
K198 IDYGkLRKDDPLTNL
Y215 AFDVAERYLDIPKML
T230 DAEDIVGTARPDEKA
Y241 DEKAIMTYVSSFyHA
S243 KAIMTYVSSFyHAFS
S244 AIMTYVSSFyHAFSG
Y246‑p MTYVSSFyHAFSGAQ
S250 SSFyHAFSGAQKAET
Y279 NEQLMEDYEKLAsDL
S284‑p EDYEKLAsDLLEWIR
K331‑ub KPPKVQEkCQLEINF
S362 PSEGRMVSDINNAWG
Y378 LEQAEKGYEEWLLNE
K398‑ac RLDHLAEkFRQKAsI
K398 RLDHLAEKFRQKAsI
K402 LAEkFRQKAsIHEAW
S404‑p EkFRQKAsIHEAWTD
T410 AsIHEAWTDGKEAML
K420‑ac KEAMLRQkDYETATL
Y422 AMLRQkDYETATLSE
S442‑p KKHEAFEsDLAAHQD
Y466 QELNELDYYDSPSVN
Y467 ELNELDYYDSPSVNA
S471 LDYYDSPSVNARCQK
T490 WDNLGALTQKRREAL
K492 NLGALTQKRREALER
Y511 LETIDQLYLEYAKRA
Y514 IDQLYLEYAKRAAPF
K516 QLYLEYAKRAAPFNN
K563‑ac ATLPDADkERLAILG
Y582 VSKIVQTYHVNMAGT
K633 QHNERLRKQFGAQAN
K649 IGPWIQTKMEEIGRI
S669 GTLEDQLSHLRQYEk
Y674 QLSHLRQYEkSIVNY
K676‑ac SHLRQYEkSIVNYKP
S677 HLRQYEkSIVNYKPK
Y681 YEkSIVNYKPKIDQL
Y708 FDNKHTNYTMEHIRV
T722 VGWEQLLTTIARTIN
T737 EVENQILTRDAKGIS
K741 QILTRDAKGISQEQM
S744 TRDAKGISQEQMNEF
- gap
S763 NHFDRDHSGTLGPEE
T765 FDRDHSGTLGPEEFK
S797 AEFARIMSIVDPNRL
T820 IDFMSRETADTDTAD
T823 MSRETADTDTADQVM
T825 RETADTDTADQVMAS
S832 TADQVMASFKILAGD
Y842 ILAGDKNYITGDELR
T844 AGDKNYITGDELRRE
Y887 MSFSTALYGEsDL__
S890‑p STALYGEsDL_____
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