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Protein Page:
MELK (human)

MELK Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis. Monomer. Interacts with ZNF622 and PPP1R8. Up-regulated in many cancers cells. Up-regulated upon treatment with radiation or 5-fluorouracil (5-FU) in colorectal cancer cells, suggesting that it might be associated with increased resistance of colorectal cells against radiation and 5- FU. Down-regulated upon siomycin A, a thiazole antibiotic, treatment, leading to inhibit tumor growth in vivo. Expressed in placenta, kidney, thymus, testis, ovary and intestine. Activated by autophosphorylation of the T-loop at Thr-167 and Ser-171: in contrast to other members of the SNF1 subfamily, phosphorylation at Thr-167 is not mediated by STK11/LKB1 but via autophosphorylation instead. Inhibited by calcium-binding. Kinase activity is also regulated by reducing agents: dithiothreitol (DTT) or reduced glutathione are required for kinase activity in vitro; such dependence is however not due to the presence of disulfide bonds. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Note: This description may include information from UniProtKB.
Protein type: CAMK group; CAMKL family; EC; EC; Kinase, protein; MELK subfamily; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); RNA splicing
Chromosomal Location of Human Ortholog: 9p13.2
Cellular Component: cell cortex; membrane; nucleus; plasma membrane
Molecular Function: calcium ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein serine/threonine kinase activity
Biological Process: apoptosis; cell proliferation; G2/M transition of mitotic cell cycle; hemopoiesis; positive regulation of apoptosis; protein amino acid autophosphorylation
Reference #:  Q14680 (UniProtKB)
Alt. Names/Synonyms: hMELK; hPK38; KIAA0175; Maternal embryonic leucine zipper kinase; MELK; pEg3 kinase; Protein kinase PK38
Gene Symbols: MELK
Molecular weight: 74,642 Da
Basal Isoelectric point: 8.92  Predict pI for various phosphorylation states
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