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Protein Page:
SLC3A2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
SLC3A2 a transmembrane solute carrier protein that transports L-type amino acids and regulates intracellular calcium levels. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L- nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. Disulfide-linked heterodimer of a glycosylated heavy chain and a non-glycosylated light chain (SLC7A5, SLC7A6, SLCA7A7, SLC7A8, SLC7A10 or SLCA7A11). Colocalizes with cadherins. Interacts with FAM57A and ICAM1. Constitutively and specifically associates with beta-1 integrins (alpha-2/beta-1, alpha-3/beta-1, alpha-5/beta-1 and alpha-6/beta-1), but minimally with alpha-4/beta-1. Expression is induced in resting peripheral blood T- lymphocytes following PHA stimulation. Expression increases at the time of maximal DNA synthesi, in fibroblasts stimulated to divide. Expression and the uptake of leucine is stimulated in mononuclear, cytotrophoblast-like choriocarcinoma cells by combined treatment with PMA and calcium ionophore. Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid- trimester stage. Belongs to the SLC3A transporter family. 4 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Transporter; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: apical plasma membrane; cell surface; cytoplasm; integral to membrane; melanosome; membrane; nucleus; plasma membrane
Molecular Function: calcium:sodium antiporter activity; catalytic activity; cation binding; double-stranded RNA binding; neutral amino acid transmembrane transporter activity; protein binding
Biological Process: amino acid transport; calcium ion transport; carbohydrate metabolic process; cell growth; leukocyte migration; response to exogenous dsRNA; tryptophan transport
Reference #:  P08195 (UniProtKB)
Gene Symbols: SLC3A2
Molecular weight: 67,994 Da
Basal Isoelectric point: 4.89  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

SLC3A2

Protein Structure Not Found.
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Download ChimeraX Script


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Sites Implicated In
cell adhesion, altered: S406‑p, S408‑p, S410‑p, S527‑p, S531‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S9‑p ELQPPEAsIAVVSIP
0 13 S103‑p AEVTGTMsQDtEVDM
0 3 T106‑p TGTMsQDtEVDMkEV
0 1 K111‑ub QDtEVDMkEVELNEL
1 0 K111 QDtEVDMKEVELNEL
1 0 K122 LNELEPEKQPMNAAs
0 4 K122‑ub LNELEPEkQPMNAAs
0 2 S129‑p kQPMNAAsGAAMsLA
0 8 S134‑p AAsGAAMsLAGAEkN
0 12 K140‑ub MsLAGAEkNGLVkIk
0 5 K145‑ub AEkNGLVkIkVAEDE
0 97 K147‑ub kNGLVkIkVAEDEAE
0 7 K160‑ub AEAAAAAkFtGLskE
0 1 T162‑p AAAAAkFtGLskEEL
0 14 S165‑p AAkFtGLskEELLkV
0 2 K166‑ac AkFtGLskEELLkVA
0 77 K166‑ub AkFtGLskEELLkVA
0 1 K166‑sc AkFtGLskEELLkVA
0 4 K171‑ub LskEELLkVAGsPGW
0 2 S175‑p ELLkVAGsPGWVRtR
0 1 T181‑p GsPGWVRtRWALLLL
0 2 K245‑ub AGNLAGLkGRLDyLS
0 1 K245‑sc AGNLAGLkGRLDyLS
0 1 Y250‑p GLkGRLDyLSSLKVK
0 1 K255 LDyLSSLKVKGLVLG
0 1 K269 GPIHKNQKDDVAQTD
0 1 K269‑ub GPIHKNQkDDVAQTD
0 1 K269‑sc GPIHKNQkDDVAQTD
0 1 K287‑ub IDPNFGSkEDFDsLL
0 1 D291 FGSkEDFDsLLQsAk
0 1 S292‑p GSkEDFDsLLQsAkK
0 3 S296‑p DFDsLLQsAkKKSIR
0 1 K298‑ub DsLLQsAkKKSIRVI
0 1 K298‑sc DsLLQsAkKKSIRVI
0 1 T309‑p IRVILDLtPNyRGEN
0 1 Y312‑p ILDLtPNyRGENsWF
0 1 S317‑p PNyRGENsWFstQVD
0 1 S320‑p RGENsWFstQVDTVA
0 1 T321‑p GENsWFstQVDTVAT
0 1 K329‑ub QVDTVATkVKDALEF
0 1 K354‑ub VRDIENLkDASSFLA
0 4 K368‑ub AEWQNITkGFSEDRL
0 1 S402‑p NKDLLLTssyLsDsG
0 1 S403‑p KDLLLTssyLsDsGs
0 1 Y404‑p DLLLTssyLsDsGsT
1 1 S406‑p LLTssyLsDsGsTGE
1 0 S408‑p TssyLsDsGsTGEHT
1 0 S410‑p syLsDsGsTGEHTkS
0 1 K416‑ub GsTGEHTkSLVTQYL
0 1 T442‑p LSQARLLtsFLPAQL
0 1 S443‑p SQARLLtsFLPAQLL
0 1 S513‑p NMTVKGQsEDPGsLL
0 1 S518‑p GQsEDPGsLLsLFRR
0 1 S521‑p EDPGsLLsLFRRLsD
1 0 S527‑p LsLFRRLsDQRsKER
1 0 S531‑p RRLsDQRsKERSLLH
0 1 S598‑p AKADLLLsTQPGREE
0 3 S607‑p QPGREEGsPLELERL
0 3 K615‑ub PLELERLkLEPHEGL
  SLC3A2 iso2  
- gap
S2 ______MSQDTEVDM
T5 ___MSQDTEVDMKEV
K10 QDTEVDMKEVELNEL
K10 QDTEVDMKEVELNEL
K21 LNELEPEKQPMNAAS
K21 LNELEPEKQPMNAAS
S28 KQPMNAASGAAMSLA
S33 AASGAAMSLAGAEKN
K39 MSLAGAEKNGLVKIK
K44 AEKNGLVKIKVAEDE
K46 KNGLVKIKVAEDEAE
K59 AEAAAAAKFTGLSKE
T61 AAAAAKFTGLSKEEL
S64 AAKFTGLSKEELLKV
K65 AKFTGLSKEELLKVA
K65 AKFTGLSKEELLKVA
K65 AKFTGLSKEELLKVA
K70 LSKEELLKVAGSPGW
S74 ELLKVAGSPGWVRTR
T80 GSPGWVRTRWALLLL
K144 AGNLAGLKGRLDYLS
K144 AGNLAGLKGRLDYLS
Y149 GLKGRLDYLSSLKVK
K154 LDYLSSLKVKGLVLG
K168 GPIHKNQKDDVAQTD
K168 GPIHKNQKDDVAQTD
K168 GPIHKNQKDDVAQTD
K186 IDPNFGSKEDFDSLL
D190 FGSKEDFDSLLQSAK
S191 GSKEDFDSLLQSAKK
S195 DFDSLLQSAKKKSIR
K197 DSLLQSAKKKSIRVI
K197 DSLLQSAKKKSIRVI
T208 IRVILDLTPNYRGEN
Y211 ILDLTPNYRGENSWF
S216 PNYRGENSWFSTQVD
S219 RGENSWFSTQVDTVA
T220 GENSWFSTQVDTVAT
K228 QVDTVATKVKDALEF
K253 VRDIENLKDASSFLA
K267 AEWQNITKGFSEDRL
S301 NKDLLLTSSYLSDSG
S302 KDLLLTSSYLSDSGS
Y303 DLLLTSSYLSDSGST
S305 LLTSSYLSDSGSTGE
S307 TSSYLSDSGSTGEHT
S309 SYLSDSGSTGEHTKS
K315 GSTGEHTKSLVTQYL
T341 LSQARLLTSFLPAQL
S342 SQARLLTSFLPAQLL
S412 NMTVKGQSEDPGSLL
S417 GQSEDPGSLLSLFRR
S420 EDPGSLLSLFRRLSD
S426 LSLFRRLSDQRSKER
S430 RRLSDQRSKERSLLH
S497 AKADLLLSTQPGREE
S506 QPGREEGSPLELERL
K514 PLELERLKLEPHEGL
  SLC3A2 iso3  
S9 ELQPPEASIAVVSIP
S41 GDDSGTMSQDTEVDM
T44 SGTMSQDTEVDMkEV
K49‑ub QDTEVDMkEVELNEL
K49 QDTEVDMKEVELNEL
K60 LNELEPEKQPMNAAS
K60 LNELEPEKQPMNAAS
S67 KQPMNAASGAAMSLA
S72 AASGAAMSLAGAEKN
K78 MSLAGAEKNGLVKIK
K83 AEKNGLVKIKVAEDE
K85 KNGLVKIKVAEDEAE
K98 AEAAAAAKFTGLSKE
T100 AAAAAKFTGLSKEEL
S103 AAKFTGLSKEELLKV
K104 AKFTGLSKEELLKVA
K104 AKFTGLSKEELLKVA
K104 AKFTGLSKEELLKVA
K109 LSKEELLKVAGSPGW
S113 ELLKVAGSPGWVRTR
T119 GSPGWVRTRWALLLL
K183 AGNLAGLKGRLDYLS
K183 AGNLAGLKGRLDYLS
Y188 GLKGRLDYLSSLKVK
K193 LDYLSSLKVKGLVLG
K207 GPIHKNQKDDVAQTD
K207 GPIHKNQKDDVAQTD
K207 GPIHKNQKDDVAQTD
K225 IDPNFGSKEDFDSLL
D229 FGSKEDFDSLLQSAK
S230 GSKEDFDSLLQSAKK
S234 DFDSLLQSAKKKSIR
K236 DSLLQSAKKKSIRVI
K236 DSLLQSAKKKSIRVI
T247 IRVILDLTPNYRGEN
Y250 ILDLTPNYRGENSWF
S255 PNYRGENSWFSTQVD
S258 RGENSWFSTQVDTVA
T259 GENSWFSTQVDTVAT
K267 QVDTVATKVKDALEF
K292 VRDIENLKDASSFLA
K306 AEWQNITKGFSEDRL
S340 NKDLLLTSSYLSDSG
S341 KDLLLTSSYLSDSGS
Y342 DLLLTSSYLSDSGST
S344 LLTSSYLSDSGSTGE
S346 TSSYLSDSGSTGEHT
S348 SYLSDSGSTGEHTKS
K354 GSTGEHTKSLVTQYL
T380 LSQARLLTSFLPAQL
S381 SQARLLTSFLPAQLL
S451 NMTVKGQSEDPGSLL
S456 GQSEDPGSLLSLFRR
S459 EDPGSLLSLFRRLSD
S465 LSLFRRLSDQRSKER
S469 RRLSDQRSKERSLLH
S536 AKADLLLSTQPGREE
S545 QPGREEGSPLELERL
K553 PLELERLKLEPHEGL
  SLC3A2 iso4  
S9 ELQPPEASIAVVSIP
S134 AGITGTMSQDTEVDM
T137 TGTMSQDTEVDMKEV
K142 QDTEVDMKEVELNEL
K142 QDTEVDMKEVELNEL
K153 LNELEPEKQPMNAAS
K153 LNELEPEKQPMNAAS
S160 KQPMNAASGAAMSLA
S165 AASGAAMSLAGAEKN
K171 MSLAGAEKNGLVKIK
K176 AEKNGLVKIKVAEDE
K178 KNGLVKIKVAEDEAE
K191 AEAAAAAKFTGLSKE
T193 AAAAAKFTGLSKEEL
S196 AAKFTGLSKEELLKV
K197 AKFTGLSKEELLKVA
K197 AKFTGLSKEELLKVA
K197 AKFTGLSKEELLKVA
K202 LSKEELLKVAGSPGW
S206 ELLKVAGSPGWVRTR
T212 GSPGWVRTRWALLLL
K276 AGNLAGLKGRLDYLS
K276 AGNLAGLKGRLDYLS
Y281 GLKGRLDYLSSLKVK
K286 LDYLSSLKVKGLVLG
K300 GPIHKNQKDDVAQTD
K300 GPIHKNQKDDVAQTD
K300 GPIHKNQKDDVAQTD
K318 IDPNFGSKEDFDSLL
D322 FGSKEDFDSLLQSAK
S323 GSKEDFDSLLQSAKK
S327 DFDSLLQSAKKKSIR
K329 DSLLQSAKKKSIRVI
K329 DSLLQSAKKKSIRVI
T340 IRVILDLTPNYRGEN
Y343 ILDLTPNYRGENSWF
S348 PNYRGENSWFSTQVD
S351 RGENSWFSTQVDTVA
T352 GENSWFSTQVDTVAT
K360 QVDTVATKVKDALEF
K385 VRDIENLKDASSFLA
K399 AEWQNITKGFSEDRL
S433 NKDLLLTSSYLSDSG
S434 KDLLLTSSYLSDSGS
Y435 DLLLTSSYLSDSGST
S437 LLTSSYLSDSGSTGE
S439 TSSYLSDSGSTGEHT
S441 SYLSDSGSTGEHTKS
K447 GSTGEHTKSLVTQYL
T473 LSQARLLTSFLPAQL
S474 SQARLLTSFLPAQLL
S544 NMTVKGQSEDPGSLL
S549 GQSEDPGSLLSLFRR
S552 EDPGSLLSLFRRLSD
S558 LSLFRRLSDQRSKER
S562 RRLSDQRSKERSLLH
S629 AKADLLLSTQPGREE
S638 QPGREEGSPLELERL
K646 PLELERLKLEPHEGL
  mouse

 
- gap
S2‑p ______MsQDTEVDM
T5 ___MsQDTEVDMKDV
K10 QDTEVDMKDVELNEL
K10 QDTEVDMKDVELNEL
K21 LNELEPEKQPMNAAD
K21 LNELEPEKQPMNAAD
D28 KQPMNAADGAAAGEk
- gap
K35‑ub DGAAAGEkNGLVKIk
K40 GEkNGLVKIkVAEDE
K42‑ub kNGLVKIkVAEDETE
K53‑ub DETEAGVkFTGLskE
T55 TEAGVkFTGLskEEL
S58‑p GVkFTGLskEELLKV
K59 VkFTGLsKEELLKVA
K59‑ub VkFTGLskEELLKVA
K59 VkFTGLsKEELLKVA
K64 LskEELLKVAGSPGW
S68 ELLKVAGSPGWVRTR
T74 GSPGWVRTRWALLLL
K138 AGGIAGLKSHLEYLS
K138 AGGIAGLKSHLEYLS
Y143 GLKSHLEYLSTLKVK
K148 LEYLSTLKVKGLVLG
K162 GPIHKNQKDEINETD
K162 GPIHKNQKDEINETD
K162 GPIHKNQKDEINETD
Q180 INPTLGSQEDFKDLL
K184 LGSQEDFKDLLQSAK
D185 GSQEDFKDLLQSAKK
S189 DFKDLLQSAKKKSIH
K191 KDLLQSAKKKSIHII
K191 KDLLQSAKKKSIHII
T202 IHIILDLTPNYQGQN
Y205 ILDLTPNYQGQNAWF
A210 PNYQGQNAWFLPAQA
L213 QGQNAWFLPAQADIV
A215 QNAWFLPAQADIVAT
K223 QADIVATKMKEALSS
M248 FRDVGKLMNAPLYLA
K262‑ub AEWQNITkNLSEDRL
S296 TSDLLLTSSYLSNST
S297 SDLLLTSSYLSNSTF
Y298 DLLLTSSYLSNSTFT
S300 LLTSSYLSNSTFTGE
S302 TSSYLSNSTFTGERT
F304 SYLSNSTFTGERTES
E310 TFTGERTESLVTRFL
A336 VSQAGLLADFIPDHL
D337 SQAGLLADFIPDHLL
N406 NMTVKGQNEDPGSLL
S411 GQNEDPGSLLTQFRR
T414 EDPGSLLTQFRRLSD
S420 LTQFRRLSDLRGKER
G424 RRLSDLRGKERSLLH
S491 ASAKLLLSTDSARQS
T503 RQSREEDTSLKLENL
S511 SLKLENLSLNPYEGL
  rat

 
- gap
S2‑p ______MsQDtEVDM
T5‑p ___MsQDtEVDMkDV
K10 QDtEVDMKDVELNEL
K10‑ac QDtEVDMkDVELNEL
K21‑ac LNELEPEkQPMNAAD
K21 LNELEPEKQPMNAAD
D28 kQPMNAADGAAAGEK
- gap
K35 DGAAAGEKNGLVKIK
K40 GEKNGLVKIKVAEDE
K42 KNGLVKIKVAEDEAE
K53 DEAEAGVKFTGLskE
T55 AEAGVKFTGLskEEL
S58‑p GVKFTGLskEELLKV
K59‑ac VKFTGLskEELLKVA
K59 VKFTGLsKEELLKVA
K59 VKFTGLsKEELLKVA
K64 LskEELLKVAGSPGW
S68 ELLKVAGSPGWVRTR
T74 GSPGWVRTRWALLLL
K138 ARGIAGLKNHLEYLS
K138 ARGIAGLKNHLEYLS
Y143 GLKNHLEYLSTLkVK
K148‑ac LEYLSTLkVKGLVLG
K162‑ac GPIHKNQkDEVNETD
K162 GPIHKNQKDEVNETD
K162 GPIHKNQKDEVNETD
Q180 IDPDLGSQEDFkDLL
K184‑ac LGSQEDFkDLLQSAK
D185 GSQEDFkDLLQSAKK
S189 DFkDLLQSAKKKSIH
K191 kDLLQSAKKKSIHII
K191 kDLLQSAKKKSIHII
T202 IHIILDLTPNYKGQN
Y205 ILDLTPNYKGQNAWF
A210 PNYKGQNAWFLPPQA
L213 KGQNAWFLPPQADIV
P215 QNAWFLPPQADIVAT
K223 QADIVATKMKEALSS
A248 VRDVGKLANASLYLA
K262 AEWQNITKNFSEDRL
S296 TSDLLLTSSYLSQPV
S297 SDLLLTSSYLSQPVF
Y298 DLLLTSSYLSQPVFT
S300 LLTSSYLSQPVFTGE
P302 TSSYLSQPVFTGEHA
F304 SYLSQPVFTGEHAEL
E310 VFTGEHAELLVIKYL
T336 VSQAGLLTSFIPAQF
S337 SQAGLLTSFIPAQFL
N407 NMTVKGQNEDPGSLL
S412 GQNEDPGSLLTQFRR
T415 EDPGSLLTQFRRLSD
S421 LTQFRRLSDLRGKER
G425 RRLSDLRGKERSLLH
S492 ASANLLLSTDSTRLS
T504 RLSREEGTSLSLENL
S512 SLSLENLSLNPYEGL
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