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Protein Page:
LEF-1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
LEF-1 a transcription factor of the TCF/LEF family. Participates in the Wnt signaling pathway and regulates T-cell receptor alpha-C enhancer function. Activates transcription of target genes in the presence of CTNNB1 and EP300. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. Deletions in LEF1 have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Four human isoforms produced by alternative promoter usage and alternative splicing have been described. Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Note: This description may include information from UniProtKB.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 4q23-q25
Cellular Component: cytoplasm; nucleoplasm; nucleus; transcription factor complex
Molecular Function: beta-catenin binding; caspase inhibitor activity; chromatin binding; DNA bending activity; DNA binding; estrogen receptor activity; estrogen receptor binding; gamma-catenin binding; histone binding; histone deacetylase binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding
Biological Process: alpha-beta T cell differentiation; anatomical structure regression; B cell proliferation; BMP signaling pathway; dentate gyrus development; embryonic limb morphogenesis; epithelial to mesenchymal transition; eye pigmentation; forebrain neuroblast division; forebrain neuron differentiation; formation of radial glial scaffolds; hypothalamus development; kidney development; mammary gland development; muscle fiber development; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of cell-cell adhesion; negative regulation of DNA binding; negative regulation of interleukin-13 production; negative regulation of interleukin-4 production; negative regulation of interleukin-5 production; negative regulation of striated muscle development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neural crest cell migration; neutrophil differentiation; odontogenesis of dentine-containing teeth; organ regeneration; osteoblast differentiation; palate development; paraxial mesoderm formation; patterning of blood vessels; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of granulocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; radial glial cell differentiation in the forebrain; regulation of cell-cell adhesion; regulation of striated muscle development; response to lithium ion; sensory perception of taste; skin development; somitogenesis; sprouting angiogenesis; steroid hormone mediated signaling; T cell receptor V(D)J recombination; T-helper 1 cell differentiation; tongue development; transcription from RNA polymerase II promoter; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin
Reference #:  Q9UJU2 (UniProtKB)
Alt. Names/Synonyms: DKFZp586H0919; FLJ46390; LEF-1; LEF1; Lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha; TCF1-alpha; TCF1ALPHA
Gene Symbols: LEF1
Molecular weight: 44,201 Da
Basal Isoelectric point: 6.9  Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics  |  Wnt/ß-Catenin Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

LEF-1

Protein Structure Not Found.


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Sites Implicated In
transcription, induced: S42‑p, S61‑p, T155‑p, S166‑p
transcription, inhibited: T155‑p, S166‑p
molecular association, regulation: S42‑p, S61‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S5‑p ___MPQLsGGGGGGG
1 6 S42‑p EKIFAEIsHPEEEGD
1 0 S61‑p KSSLVNEsEIIPASN
0 1 S79‑p VARQAQTsQEPyHDk
0 7 Y83‑p AQTsQEPyHDkAREH
0 2 K86‑ac sQEPyHDkAREHPDD
0 3 K95‑ac REHPDDGkHPDGGLy
0 1 K95‑ub REHPDDGkHPDGGLy
0 4 Y102‑p kHPDGGLyNKGPSYS
1 0 Y114‑p SYSSYSGyIMMPNMN
1 4 S132‑p YMSNGSLsPPIPRTS
3 11 T155‑p SHAVHPLtPLItysD
0 1 T159‑p HPLtPLItysDEHFs
0 2 Y160‑p PLtPLItysDEHFsP
0 1 S161‑p LtPLItysDEHFsPG
2 1 S166‑p tysDEHFsPGSHPSH
0 2 T195‑p PPAPDIPtFyPLsPG
0 1 Y197‑p APDIPtFyPLsPGGV
1 20 S200‑p IPtFyPLsPGGVGQI
0 25 T208‑p PGGVGQItPPLGWQG
0 1 S238‑p SSLSVDTsMsRFSHH
0 1 S240‑p LSVDTsMsRFSHHMI
1 1 T265‑p IPHPAIVtPQVKQEH
1 0 K269 AIVtPQVKQEHPHTD
0 1 K283‑ub DSDLMHVkPQHEQRK
0 1 K295‑ub QRKEQEPkRPHIKkP
0 1 K301‑ub PkRPHIKkPLNAFML
0 4 K324‑ub VVAECTLkESAAINQ
0 9 K347‑ub LSREEQAkYYELARK
0 12 Y363‑p RQLHMQLyPGWSARD
0 5 K382‑ub KKKRKREkLQEsASG
0 2 S386‑p KREkLQEsASGtGPR
0 2 T390‑p LQEsASGtGPRMTAA
0 1 - gap
0 1 - gap
  LEF-1 iso6  
S5 ___MPQLSGGGGGGG
S42 EKIFAEISHPEEEGD
S61 KSSLVNESEIIPASN
S79 VARQAQTSQEPYHDK
Y83 AQTSQEPYHDKAREH
K86 SQEPYHDKAREHPDD
K95 REHPDDGKHPDGGLY
K95 REHPDDGKHPDGGLY
Y102 KHPDGGLYNKGPSYS
Y114 SYSSYSGYIMMPNMN
S132 YMSNGSLSPPIPRTS
T155 SHAVHPLTPLITYSD
T159 HPLTPLITYSDEHFS
Y160 PLTPLITYSDEHFSP
S161 LTPLITYSDEHFSPG
S166 TYSDEHFSPGSHPSH
T195 PPAPDIPTFYPLsPG
Y197 APDIPTFYPLsPGGV
S200‑p IPTFYPLsPGGVGQI
T208‑p PGGVGQItPPLGWFS
- gap
- gap
T237‑p IPHPAIVtPQVKQEH
K241 AIVtPQVKQEHPHTD
K255 DSDLMHVKPQHEQRK
K267 QRKEQEPKRPHIKKP
K273 PKRPHIKKPLNAFML
K296 VVAECTLKESAAINQ
K319 LSREEQAKYYELARK
Y335 RQLHMQLYPGWSARD
K354 KKKRKREKLQESASG
S358 KREKLQESASGGkRS
- gap
K363‑ac QESASGGkRSsFPTC
S366‑p ASGGkRSsFPTCKAK
  mouse

► Hide Isoforms
 
S5 ___MPQLSGGGGGGD
S40 EKIFAEISHPEEEGD
S59 KSSLVNESEIIPASN
S77 VVRQAPSSQEPYHDK
Y81 APSSQEPYHDKAREH
K84 SQEPYHDKAREHPDE
K93 REHPDEGKHPDGGLY
K93 REHPDEGKHPDGGLY
Y100 KHPDGGLYNKGPSYS
Y112 SYSSYSGYIMMPNMN
S130 YMSNGSLSPPIPRTS
T153 SHAVHPLTPLITYSD
T157 HPLTPLITYSDEHFS
Y158 PLTPLITYSDEHFSP
S159 LTPLITYSDEHFSPG
S164 TYSDEHFSPGSHPSH
T193 PPAPEIPTFYPLSPG
Y195 APEIPTFYPLSPGGV
S198 IPTFYPLSPGGVGQI
T206 PGGVGQITPPIGWQG
S236 SSLSGDTSMSRFSHH
S238 LSGDTSMSRFSHHMI
T263 IPHPAIVTPQVkQEH
K267‑sm AIVTPQVkQEHPHTD
K281 DSDLMHVKPQHEQRK
K293 QRKEQEPKRPHIKKP
K299 PKRPHIKKPLNAFML
K322 VVAECTLKESAAINQ
K345 LSREEQAKYYELARK
Y361 RQLHMQLYPGWSARD
K380 KKKRKREKLQESTSG
S384 KREKLQESTSGTGPR
T388 LQESTSGTGPRMTAA
- gap
- gap
  LEF-1 iso3  
S5 ___MPQLSGGGGGGD
S40 EKIFAEISHPEEEGD
S59 KSSLVNESEIIPASN
S77 VVRQAPSSQEPYHDK
Y81 APSSQEPYHDKAREH
K84 SQEPYHDKAREHPDE
K93 REHPDEGKHPDGGLY
K93 REHPDEGKHPDGGLY
Y100 KHPDGGLYNKGPSYS
Y112 SYSSYSGYIMMPNMN
S130 YMSNGSLSPPIPRTS
T153 SHAVHPLTPLITYSD
T157 HPLTPLITYSDEHFS
Y158 PLTPLITYSDEHFSP
S159 LTPLITYSDEHFSPG
S164 TYSDEHFSPGSHPSH
T193 PPAPEIPTFYPLSPG
Y195 APEIPTFYPLSPGGV
S198 IPTFYPLSPGGVGQI
T206 PGGVGQITPPIGWFS
- gap
- gap
T235 IPHPAIVTPQVKQEH
K239 AIVTPQVKQEHPHTD
K253 DSDLMHVKPQHEQRK
K265 QRKEQEPKRPHIKKP
K271 PKRPHIKKPLNAFML
K294 VVAECTLKESAAINQ
K317 LSREEQAKYYELARK
Y333 RQLHMQLYPGWSARD
K352 KKKRKREKLQEsTSG
S356‑p KREKLQEsTSGGKRS
- gap
K361 QEsTSGGKRSSFPTC
S364 TSGGKRSSFPTCKAK
  rat

 
S5 ___MPQLSGGGGGGD
S40 EKIFAEISHPEEEGD
S59 KSSLVNESEIIPASN
S77 VVGQTQSSQEPYHDk
Y81 TQSSQEPYHDkAREH
K84‑ac SQEPYHDkAREHPDD
K93‑ac REHPDDGkHPDGGLY
K93 REHPDDGKHPDGGLY
Y100 kHPDGGLYNKGPSYS
Y112 SYSSYSGYIMMPNMN
S130 YMSNGSLSPPIPRTS
T153 SHAVHPLTPLITYSD
T157 HPLTPLITYSDEHFS
Y158 PLTPLITYSDEHFSP
S159 LTPLITYSDEHFSPG
S164 TYSDEHFSPGSHPSH
T193 PPAPEMPTFYPLSPG
Y195 APEMPTFYPLSPGGV
S198 MPTFYPLSPGGVGQI
T206 PGGVGQITPPLGWQG
S236 SSLSGDTSMSRFSHH
S238 LSGDTSMSRFSHHMI
T263 IPHPAIVTPQVKQEH
K267 AIVTPQVKQEHPHTD
K281 DSDLMHVKPEHEQRK
K293 QRKEQEPKRPHIKKP
K299 PKRPHIKKPLNAFML
K322 VVAECTLKESAAINQ
K345 LSREEQAKYYELARK
Y361 RQLHMQLYPGWSARD
K380 KKKRKREKLQESTSG
S384 KREKLQESTSGTGPR
T388 LQESTSGTGPRMTAA
- gap
- gap
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