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Protein Page:
NKX2-5 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NKX2-5 Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. Interacts with HIPK1 and HIPK2, but not HIPK3. Interacts with the C-terminal zinc finger of GATA4 through its homeobox domain. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with FBLIM1. Expressed only in the heart. Belongs to the NK-2 homeobox family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 5q34
Cellular Component: cytoplasm; nucleus; transcription factor complex
Molecular Function: chromatin binding; DNA binding; protein binding; protein heterodimerization activity; protein homodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding
Biological Process: adult heart development; atrial cardiac muscle cell development; BMP signaling pathway; cardiac muscle cell differentiation; cardiac muscle cell proliferation; cardiac muscle contraction; cardiac muscle morphogensis; cell differentiation; embryonic heart tube development; heart looping; heart morphogenesis; hemopoiesis; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; pharyngeal system development; positive regulation of cardioblast differentiation; positive regulation of cell proliferation; positive regulation of heart contraction; positive regulation of neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of cardiac muscle cell proliferation; sarcomere organization; spleen development; thyroid gland development; transcription from RNA polymerase II promoter; vasculogenesis; ventricular cardiac muscle cell development; ventricular cardiac myofibril development; Wnt receptor signaling pathway through beta-catenin
Disease: Atrial Septal Defect 7 With Or Without Atrioventricular Conduction Defects; Conotruncal Heart Malformations; Hypoplastic Left Heart Syndrome 2; Hypothyroidism, Congenital, Nongoitrous, 5; Tetralogy Of Fallot; Ventricular Septal Defect 3
Reference #:  P52952 (UniProtKB)
Alt. Names/Synonyms: Cardiac-specific homeobox; cardiac-specific homeobox 1; CHNG5; CSX; CSX1; FLJ52202; FLJ97166; FLJ97195; FLJ97197; FLJ99536; Homeobox protein CSX; Homeobox protein NK-2 homolog E; Homeobox protein Nkx-2.5; NK2 transcription factor related, locus 5 (Drosophila); NKX2-5; NKX2.5; NKX25; NKX2E; NKX4-1; tinman paralog
Gene Symbols: NKX2-5
Molecular weight: 34,918 Da
Basal Isoelectric point: 9.46  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NKX2-5

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
1 0 K52 SCMLAAFKPEAYAGP
0 2 S78‑p AELGRAPsPAKCASA
0 1 - gap
0 1 - gap
1 1 S164‑p FKQQRYLsAPERDQL
0 1 T180‑p SVLKLTStQVKIWFQ
0 1 R225‑m2 IAVPVLVrDGKPCLG
  NKX2-5 iso2  
K52 SCMLAAFKPEAYAGP
S78 AELGRAPSPAKCASA
S139‑p PDFLQMLsEtCRWLP
T141‑p FLQMLsEtCRWLPVH
- gap
- gap
- gap
  mouse

 
K51‑sm SCMLAAFkPEAYSGP
S77‑p AEMGPAPsPPKCSPA
- gap
- gap
S163‑p FKQQRYLsAPERDQL
T179 SVLKLTSTQVKIWFQ
R221 IAVPVLVRDGKPCLG
  rat

 
K51 SCMLAAFKPDGYSGP
S77 AELGPAPSPPKCSPA
- gap
- gap
S163 FKQQRYLSPAERDQL
T179 SVLKLTSTQVKIWFQ
R221 IAVPVLVRDGKPCLG
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