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Protein Page:
MED12 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
MED12 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3. Ubiquitous. Belongs to the Mediator complex subunit 12 family. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: Xq13
Cellular Component: centrosome; membrane; nucleolus; nucleoplasm; nucleus; Srb-mediator complex; ubiquitin ligase complex
Molecular Function: beta-catenin binding; chromatin binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; protein C-terminus binding; protein domain specific binding; receptor activity; thyroid hormone receptor binding; transcription coactivator activity; transcription cofactor activity; vitamin D receptor binding
Biological Process: androgen receptor signaling pathway; embryonic neurocranium morphogenesis; endoderm development; gene expression; heart development; negative regulation of Wnt receptor signaling pathway; neural tube closure; oligodendrocyte development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein ubiquitination; Schwann cell development; spinal cord development; stem cell maintenance; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, planar cell polarity pathway
Disease: Lujan-fryns Syndrome; Ohdo Syndrome, X-linked; Opitz-kaveggia Syndrome
Reference #:  Q93074 (UniProtKB)
Alt. Names/Synonyms: Activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; CAGH45; FGS1; HOPA; human opposite paired; KIAA0192; MED12; Mediator complex subunit 12; Mediator of RNA polymerase II transcription subunit 12; mediator of RNA polymerase II transcription, subunit 12 homolog; OKS; OPA-containing protein; OPA1; Thyroid hormone receptor-associated protein complex 230 kDa component; thyroid hormone receptor-associated protein, 230 kDa subunit; TNRC11; TRAP230; trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit); Trinucleotide repeat-containing gene 11 protein
Gene Symbols: MED12
Molecular weight: 243,081 Da
Basal Isoelectric point: 6.63  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

MED12

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 K15 SYEHRPLKRPRLGPP
0 1 K68‑ub NVSFNPAkISSNFSS
0 2 K80‑ub FSSIIAEkLRCNTLP
0 1 P94 PDTGRRKPQVNQKDN
0 1 S137 AKKVPIFSKKEEVFG
0 1 Y166‑p LIKMTCAyYAAISET
0 1 T302‑p RRLAYFCtRRLALQL
0 1 K429‑ub REIEQQIkERGQAVE
0 1 K443‑ub EVRWSFDkCQEATAG
0 1 S471‑p DSHSFERsDFsNsLD
0 1 S474‑p SFERsDFsNsLDsLC
0 1 S476‑p ERsDFsNsLDsLCNR
0 2 S479‑p DFsNsLDsLCNRIFG
0 1 K527‑ub HRAMVVAkLLEKRQA
0 38 S635‑p APGPRPPsPFDDPAD
0 2 A641 PsPFDDPADDPEHKE
0 1 S665‑p EDPGLSEsMDIDPSS
0 2 S688‑p KPDFSLFsPTMPCEG
0 13 S698‑p MPCEGKGsPsPEKPD
0 12 S700‑p CEGKGsPsPEKPDVE
0 1 K762‑ub VVLFGVGkQRDDARH
0 1 K780‑ub KITKDILkVLNRkGT
0 1 K785‑ub ILkVLNRkGTAETDQ
0 2 K814‑ub LGGEDGQkRRRNRPE
0 1 K988 SCSHLKNKFGELFSD
0 1 K999‑ac LFSDFCSkVkNTIYC
0 1 K999 LFSDFCSKVkNTIYC
0 1 K1001‑ub SDFCSkVkNTIYCNV
0 1 K1194 PCQSDGNKPTVGIRS
0 9 S1258‑p EEEGGGGsGGRRQGG
0 2 S1269‑p RQGGRNIsVETASLD
0 1 K1298‑ub WVGERCLkSLCEDSN
0 1 K1414‑ub MPSSSKTkPVLSSLE
0 1 K1445‑ub SVQGHVLkAAGEELE
0 1 K1453‑ub AAGEELEkGQHLGSS
0 1 K1534‑ub QLMHEALkLRLNLVG
0 1 K1643‑ub RQLLPLPkQTRDVIT
0 1 K1664‑ub LIDTKGNkIAGFDSI
0 1 K1674‑ub GFDSIFKkEGLQVST
0 1 K1761‑ac PTLLEPEkKAPEPPK
0 1 K1761‑ub PTLLEPEkKAPEPPK
0 3 S1778‑p KPGAAPPstEERKKK
0 2 T1779‑p PGAAPPstEERKKKS
0 7 K1798‑ac KRSQPATkTEDYGMG
0 1 K1798‑ub KRSQPATkTEDYGMG
0 1 R1854 PLPAGGPRVDPYrPV
0 1 R1859‑m2 GPRVDPYrPVrLPMQ
0 1 R1862‑m2 VDPYrPVrLPMQKLP
0 1 R1899 SYKTSVYRQQQPAVP
0 11 R1899‑m2 SYKTSVYrQQQPAVP
0 1 R1910 PAVPQGQRLrQQLQQ
0 1 R1910 PAVPQGQRLrQQLQQ
0 3 R1912‑m2 VPQGQRLrQQLQQSQ
0 1 R1994‑m2 PTRHLQQrPSGYVHQ
0 1 R2015‑m2 HGLTSTQrFSHQTLQ
0 3 S2163‑p RQLQQQLsNTQPQPS
  MED12 iso2  
K15 SYEHRPLKRPRLGPP
K68 NVSFNPAKISSNFSS
K80 FSSIIAEKLRCNTLP
P94 PDTGRRKPQVNQKDN
S137 AKKVPIFSKKEEVFG
Y166 LIKMTCAYYAAISET
T302 RRLAYFCTRRLALQL
K429 REIEQQIKERGQAVE
K443 EVRWSFDKCQEATAG
S471 DSHSFERSDFSNSLD
S474 SFERSDFSNSLDSLC
S476 ERSDFSNSLDSLCNR
S479 DFSNSLDSLCNRIFG
K527 HRAMVVAKLLEKRQA
S635 APGPRPPSPFDDPAD
A641 PSPFDDPADDPEHKE
S665 EDPGLSESMDIDPSS
S688 KPDFSLFSPTMPCEG
S698 MPCEGKGSPSPEKPD
S700 CEGKGSPSPEKPDVE
K762 VVLFGVGKQRDDARH
K780 KITKDILKVLNRKGT
K785 ILKVLNRKGTAETDQ
K814 LGGEDGQKRRRNRPE
K988 SCSHLKNKFGELFSD
K999 LFSDFCSKVKNTIYC
K999 LFSDFCSKVKNTIYC
K1001 SDFCSKVKNTIYCNV
K1194 PCQSDGNKPTVGIRS
S1258 EEEGGGGSGGRRQGG
S1269 RQGGRNISVETASLD
K1298 WVGERCLKSLCEDSN
K1414 MPSSSKTKPVLSSLE
K1445 SVQGHVLKAAGEELE
K1453 AAGEELEKGQHLGSS
K1534 QLMHEALKLRLNLVG
K1643 RQLLPLPKQTRDVIT
K1664 LIDTKGNKIAGFDSI
K1674 GFDSIFKKEGLQVST
K1761 PTLLEPEKKAPEPPK
K1761 PTLLEPEKKAPEPPK
S1778 KPGAAPPSTEERKKK
T1779 PGAAPPSTEERKKKS
K1798 KRSQPATKTEDYGMG
K1798 KRSQPATKTEDYGMG
R1854 PLPAGGPRVDPYRPV
R1859 GPRVDPYRPVRLPMQ
R1862 VDPYRPVRLPMQKLP
R1899 SYKTSVYRQQQPAVP
R1899 SYKTSVYRQQQPAVP
R1910 PAVPQGQRLRQQLQA
R1910 PAVPQGQRLRQQLQA
R1912 VPQGQRLRQQLQAKI
R1997 PTRHLQQRPSGYVHQ
R2018 HGLTSTQRFSHQTLQ
S2166 RQLQQQLSNTQPQPS
  mouse

 
K15 SYEHRPLKRLRLGPP
K68 NVNFNPAKISSNFSS
K80‑ub FSSIIAEkLRCNTLS
S94‑p SDTGRRKsLMNQKDN
S137‑p AKKVPIFsKKEEVFG
Y166 LIKMTCAYYAAMSET
T303 RRLAYFCTRRLALQL
K430 REIEQQIKERGQAVE
K444 EVRWSFDKCQEATAG
S472 DSHSFERSDFSNSLD
S475 SFERSDFSNSLDSLC
S477 ERSDFSNSLDSLCNR
S480 DFSNSLDSLCNRIFG
K528 HRAMVVAKLLEKRQA
S636‑p APGPRPPsPFDDPtD
T642‑p PsPFDDPtDDPERKE
S666 EDPGLSESMDIDPSS
S689 KPDFSLFSPTMPCEG
S699‑p MPCEGKGsPsPEKPD
S701‑p CEGKGsPsPEKPDVE
K763 VVLFGVGKQRDDARH
K781 KITKDILKVLNRKGT
K786 ILKVLNRKGTAETDQ
K815‑ub LGGEDGQkRRRNRPE
K989‑ub SCSHLKSkFGELFSD
K1000 LFSDFCSKVKNTIYC
K1000‑ub LFSDFCSkVKNTIYC
K1002 SDFCSkVKNTIYCNV
K1195‑ub PCQSDGNkPTVGIRS
S1259 EEEGGGGSSGRRQGG
S1270 RQGGRNISVETASLD
K1299 WVGERCLKSLCEDSN
K1415 MPSSSKTKPVLSSLE
K1446 SVQGHVLKAAGEELE
K1454 AAGEELEKGQHLGSS
K1535 QLMHEALKLRLNLVG
K1644 HQLLPLPKQNRDVIT
K1665 LIDTKGNKIAGFDSI
K1675 GFDSIFKKEGLQVST
K1763 PALLEPEKKAPEPPK
K1763 PALLEPEKKAPEPPK
S1780 KPGAAPPSTEERKKK
T1781 PGAAPPSTEERKKKS
K1800 KRSQPATKNEDYGMG
K1800 KRSQPATKNEDYGMG
R1856‑m2 PLPAGGPrVDPYrPV
R1861‑m2 GPrVDPYrPVrLPMQ
R1864‑m2 VDPYrPVrLPMQKLP
R1901‑m1 SYKTSVYrQQQPTVP
R1901‑m2 SYKTSVYrQQQPTVP
R1912‑m1 PTVPQGQrLrQQLQA
R1912‑m2 PTVPQGQrLrQQLQA
R1914‑m2 VPQGQrLrQQLQAKI
R1999 PTRHLQQRPSGYVHQ
R2020 HGLTSTQRFSHQTLQ
S2176 RQLQQQLSNTQPQPS
  rat

 
K15‑ac SYEHRPLkRLRLGPP
K68 NVNFNPAKISSNFSS
K80 FSSIIAEKLRCNTLS
S94 SDTGRRKSLMNQKDN
S137 AKKVPIFSKKEEVFG
Y166 LIKMTCAYYAAMSET
T303 RRLAYFCTRRLALQL
K430 REIEQQIKERGQAVE
K444 EVRWSFDKCQEATAG
S472 DSHSFERSDFSNSLD
S475 SFERSDFSNSLDSLC
S477 ERSDFSNSLDSLCNR
S480 DFSNSLDSLCNRIFG
K528 HRAMVVAKLLEKRQA
S636‑p TPGPRPPsPFDDPAD
A642 PsPFDDPADDPERKE
S666 EDPGLSESMDIDPSS
S689 KPDFSLFSPTMPCEG
S699 MPCEGKGSPSPEKPD
S701 CEGKGSPSPEKPDVE
K763 VVLFGVGKQRDDARH
K781 KITKDILKVLNRKGT
K786 ILKVLNRKGTAETDQ
K815 LGGEDGQKRRRNRPE
K989 SCSHLKSKFGELFSD
K1000 LFSDFCSKVKNTIYC
K1000 LFSDFCSKVKNTIYC
K1002 SDFCSKVKNTIYCNV
K1195 PCQSDGNKPTVGIRS
S1259 EEEGGGGSSGRRQGG
S1270 RQGGRNISVETASLD
K1299 WVGERCLKSLCEDSN
K1415 MPSSSKAKPVLSSLE
K1446 SVQGHVLKAAGEELE
K1454 AAGEELEKGQHLGSS
K1535 QLMHEALKLRLNLVG
K1644 HQLLPLPKQNRDVIT
K1665 LIDTKGNKIAGFDSI
K1675 GFDSIFKKEGLQVST
K1763 PALLEPEKKAPEPPK
K1763 PALLEPEKKAPEPPK
S1780 KPGTAPPSTEERKKK
T1781 PGTAPPSTEERKKKS
K1800 KRSQPATKSEDYGMG
K1800 KRSQPATKSEDYGMG
R1856 PLPAGGPRVDPYRPV
R1861 GPRVDPYRPVRLPMQ
R1864 VDPYRPVRLPMQKLP
R1901 SYKTSVYRQQQPTVP
R1901 SYKTSVYRQQQPTVP
R1912 PTVPQGQRLRQQLQQ
R1912 PTVPQGQRLRQQLQQ
R1914 VPQGQRLRQQLQQSQ
R1996 PTRHLQQRPSGYVHQ
R2017 HGLTSTQRFSHQTLQ
S2173 RQLQQQLSNTQPQPS
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