Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteprivacy & cookiesCuration ProcessContact
logos LINCs Logo Mt Sinai Logo NIH Logo NCI Logo
Protein Page:
ATG4B (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
ATG4B Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family. 5 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Autophagy; EC 3.4.22.-; Ubiquitin conjugating system; Protease
Chromosomal Location of Human Ortholog: 2q37.3
Cellular Component: cytoplasm; cytosol
Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; endopeptidase activity; protein binding
Biological Process: autophagic vacuole formation; autophagy; C-terminal protein lipidation; macroautophagy; mitochondrion degradation; protein delipidation; protein processing; protein targeting to membrane; proteolysis
Reference #:  Q9Y4P1 (UniProtKB)
Alt. Names/Synonyms: APG4 autophagy 4 homolog B; APG4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); ATG4B; AUT-like 1 cysteine endopeptidase; AUTL1; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; Cysteine protease ATG4B; hAPG4B; KIAA0943; MGC1353
Gene Symbols: ATG4B
Molecular weight: 44,294 Da
Basal Isoelectric point: 4.91  Predict pI for various phosphorylation states
CST Pathways:  Autophagy Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ATG4B

Protein Structure Not Found.
Download PyMol Script
Download ChimeraX Script


STRING  |  cBioPortal  |  Wikipedia  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Scansite  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Sites Implicated In
autophagy, induced: S383‑p, S392‑p
enzymatic activity, induced: S383‑p, S392‑p
molecular association, regulation: S383‑p, S392‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 T7 _MDAATLTyDTLRFA
0 2 Y8‑p MDAATLTyDTLRFAE
0 2 S34 WILGRKYSIFTEKDE
0 2 S44‑p TEKDEILsDVASRLW
0 1 Y122‑p FIDRKDSyySIHQIA
0 1 Y123‑p IDRKDSyySIHQIAQ
0 1 K153‑ub NTVAQVLkKLAVFDT
0 1 S316‑p SIAELDPsIAVGFFC
1 33 S383‑p RLERFFDsEDEDFEI
1 8 S392‑p DEDFEILsL______
  mouse

 
T7‑p _MDAATLtYDTLRFA
Y8 MDAATLtYDTLRFAE
S34‑p WILGRKYsIFTEKDE
S44 TEKDEILSDVASRLW
Y122 FLDRKDSYYSIHQIA
Y123 LDRKDSYYSIHQIAQ
K153 NTVAQVLKKLAVFDT
S316 GIGELDPSIAVGFFC
S383‑p RLERFFDsEDEDFEI
S392‑p DEDFEILsL______
  rat

 
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S147 GIGELDPSIAVGFFC
S214‑p RLERFFDsEDEDFEI
S223‑p DEDFEILsL______
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.