Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A. Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1. Belongs to the helicase family. RecQ subfamily. Note: This description may include information from UniProtKB.
Protein type: EC 188.8.131.52; DNA repair, damage; DNA-binding; Cell cycle regulation; Nucleolus; Helicase
Molecular Function: 3'-5' DNA helicase activity; 3'-5' exonuclease activity; ATP binding; ATP-dependent 3'-5' DNA helicase activity; ATP-dependent DNA helicase activity; ATPase activity; bubble DNA binding; chromatin binding; DNA binding; DNA helicase activity; exonuclease activity; four-way junction helicase activity; G-quadruplex DNA binding; helicase activity; magnesium ion binding; manganese ion binding; protein binding; protein complex binding; protein homodimerization activity; Y-form DNA binding
Biological Process: aging; base-excision repair; brain development; cell aging; cellular protein metabolic process; cellular response to starvation; DNA duplex unwinding; DNA metabolic process; DNA repair; DNA replication; DNA synthesis during DNA repair; double-strand break repair; double-strand break repair via homologous recombination; double-strand break repair via synthesis-dependent strand annealing; multicellular organismal aging; nucleolus to nucleoplasm transport; positive regulation of hydrolase activity; post-translational protein modification; protein sumoylation; regulation of apoptosis; regulation of growth rate; replication fork processing; replicative cell aging; response to DNA damage stimulus; response to oxidative stress; response to UV-C; telomere maintenance
LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.