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Protein Page:
CHFR (human)

Overview
CHFR E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. Interacts with HDAC1 and HDAC2. Interacts with PML (with sumoylated form of PML). Ubiquitous. Belongs to the CHFR family. 5 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 12q24.33
Cellular Component: nucleus; PML body
Molecular Function: nucleotide binding; protein binding; ubiquitin-protein ligase activity
Biological Process: mitotic cell cycle checkpoint; modification-dependent protein catabolic process; protein polyubiquitination; ubiquitin-dependent protein catabolic process
Reference #:  Q96EP1 (UniProtKB)
Alt. Names/Synonyms: checkpoint with forkhead and ring finger domains; Checkpoint with forkhead and RING finger domains protein; CHFR; E3 ubiquitin-protein ligase CHFR; FLJ10796; FLJ33629; RING finger protein 196; RNF116; RNF196
Gene Symbols: CHFR
Molecular weight: 73,386 Da
Basal Isoelectric point: 5.71  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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CHFR

Protein Structure Not Found.
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